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All Journal Biology, Medicine, & Natural Product Chemistry Meditory : The Journal of Medical Laboratory Jurnal Peduli Masyarakat Muhammadiyah Medical Journal Hang Tuah Medical Journal Jurnal Pengabdian Masyarakat Medika Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Folia Medica Indonesiana
Widhidewi, Ni Wayan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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ANTIBACTERIAL ACTIVITY SCREENING OF BACTERIAL ISOLATES ASSOCIATED WITH SEAWEEDS Caulerpa lentillifera AR, Sunthio Barran Cia; Indraningrat, Anak Agung Gede; Widhidewi, Ni Wayan; Wijaya, Made Dharmesti; Antara, Kadek Lila; Choe, Daegeun
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.4051

Abstract

Background: Caulerpa lentillifera is a species of green algae that has been cultivated widely in Indonesia for its nutritional contents and health benefit. This seaweed builds a strong association with bacterial community which play an important role in synthesizing various secondary metabolites including antibacterial activity. Aims:The research aim to explore the symbiotic relationship between C. lentillifera and its associated bacterial community, focusing on the potential of these bacteria to produce antibacterial metabolites.Methods:Bacterial cultivation was performed on three different media namely nutrient agar, plate count agar, and zobell marine agar. The obtained bacterial isolates were identified morphologically and subsequently were screened for their antibacterial activity based on perpendicular cross streak method against Staphylococcus aureus ATCC 25923, Streptococcus mutans FNCC 0405, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 700603. Results: 20 out of 28 bacterial isolates inhibited against at least one of the bacterial targets. Bacterial isolate encoded as NAR9 showed as the most promising bacterial isolates with length inhibition of 19 mm against S. mutans FNCC 0405. Amplification of 16S rRNA gene fragment of the NAR9 bacterial isolate followed by Sanger sequencing showed that the isolate NAR9 has been phylogenetically related to Marinobacter hydrocarbonoclasticus. Conclusion: Overall, this research provides a preliminary result of the untapped potential of bacteria associated with C. lentillifera as the source of antibacterial activity. 
Identification of Pathogenic Bacteria in Food Samples from Cafeterias of a University in Denpasar, Indonesia , Anak Agung Ayu Lila Paramasatiari; , Putu Indah Budiapsari; Suryanditha, Putu Arya; Widhidewi, Ni Wayan
Folia Medica Indonesiana Vol. 58, No. 4
Publisher : Folia Medica Indonesiana

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Highlights: • Eschericia colli was the most contaminant bacteria among food samples from Warmadewa Cafeterias • Further suggestions to increase the hygiene in food processing must be offered. Abstract : Foodborne diseases still remain a problem especially in public areas. This study aimed to identify pathogenic bacteria in foods sold at the cafeterias of Universitas Warmadewa, Denpasar, Indonesia. The presence of pathogenic bacteria in the food samples was identified using the brain heart infusion (BHI) agar for the growth of Escherichia coli and Streptococcus aureus, selenite for Shigella and Salmonella, and alkaline peptone for Vibrio cholerae, then bacterial culture was conducted to identify the species. The results showed that Escherichia coli were found in two food samples, kangkong and jinggo rice, with the colony counts of 50-118. Escherichia coli was the most commonly found contaminant in food samples from the Warmadewa Cafeterias. Further suggestions must be offered to increase hygiene in the food processing by advising the stalls' owners to serve good foods and conducting regular inspections to assess the food quality.
Co-Authors , Anak Agung Ayu Lila Paramasatiari , Putu Indah Budiapsari Apsari, Putu Indah Budi AR, Sunthio Barran Cia ATWN, Anak Agung Sagung budiapsari, putu indah Choe, Daegeun ELMY SANIATHI, NI KADEK I Nengah Kapti Indraningra, Anak Agung Gede Indraningrat, Anak Agung Gede Kadek Lila Antara Made Dharmesti Wijaya Natania, Evellyne Paramasatiari, Anak Agung Ayu Lila Pramana, Made Surya Primatanti, Putu Asih PRIYANKA HERMAWAN, PUTU Purnama, Ni Kadek Ari Putu Arya Suryanditha Santoso, Putu Nia Calista Sayu Widiawati, Sayu Setiabudy, Marta Suryawan, Kadek Wijaya, I Dewa Gede Harry Wijaya, Made Indra Wijaya, Putu Austin Widyasari