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Penentuan Kadar Fenolik Total dan Uji Antioksidan Kombinasi Ekstrak Daun Kecibeling (Strobilanthes crispa (L.) Blume) dan Daun Pepaya (Carica papaya L.) Melindra Mulia; Dedek Fitriani
MASALIQ Vol 5 No 5 (2025): SEPTEMBER
Publisher : Lembaga Yasin AlSys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/masaliq.v5i5.7241

Abstract

The limited number of studies on the synergistic antioxidant potential of combined extracts from Kecibeling leaves (Strobilanthes crispa (L.) Blume) and Papaya leaves (Carica papaya L.) forms the basis of this research, despite its significant implications for the development of safe natural antioxidant products. This study aims to determine the total phenolic content and evaluate the antioxidant activity of various combination ratios of Kecibeling and Papaya leaf extracts. A quantitative experimental approach was employed, using maceration extraction with 96% ethanol as the solvent. Data were obtained through the Folin–Ciocalteu assay to determine total phenolic content and the DPPH method to assess antioxidant activity, analyzed via spectrophotometry and linear regression to calculate IC₅₀ values. The results show that the 75:25 ratio (Kecibeling:Papaya) yielded the highest total phenolic content at 71.35 mg GAE/g and exhibited the strongest antioxidant activity with an IC₅₀ value of 62.02 µg/mL, categorized as a strong antioxidant. These findings support the theory that combining phenolic compounds from different plant sources can enhance their biological activity. Thus, the combination of Kecibeling and Papaya leaf extracts proved superior to single extracts in terms of antioxidant efficacy. The implications of this study include contributions to the literature on phytochemical synergy and practical recommendations for the herbal and functional food industries in formulating natural ingredient combinations.
Uji Kualitatif Fitokimia Ekstrak Daun Alpukat (Persea americana Mill.) dan Daun Belimbing Wuluh (Averrhoa bilimbi L.) serta Kombinasinya Azizah Lailatul Nadhifah; Melindra Mulia
MASALIQ Vol 5 No 5 (2025): SEPTEMBER
Publisher : Lembaga Yasin AlSys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/masaliq.v5i5.7301

Abstract

Plants are an important source of natural medicinal compounds, particularly through secondary metabolites with biological activity. Avocado leaves (Persea americana) and belimbing wuluh leaves (Averrhoa bilimbi) are known to contain such compounds and hold potential as natural medicines. This study aims to identify the secondary metabolite content in combined extracts of avocado and belimbing wuluh leaves through phytochemical testing. The method used was qualitative phytochemical analysis to detect the presence of active compounds in each extract and in their combination. The results show that avocado leaf extract contains flavonoids, phenols, alkaloids, saponins, and steroids, while belimbing wuluh leaf extract contains phenols, flavonoids, alkaloids, and steroids but lacks saponins. After combining the two extracts, the test results indicated the presence of flavonoids and phenols. These findings suggest that the combined extracts retain key bioactive compounds with potential pharmacological effects. The study concludes that the combination of avocado and belimbing wuluh leaf extracts contains secondary metabolites that may be further developed as natural medicinal ingredients. The implications open opportunities for future research to examine the pharmacological activity and therapeutic potential of this plant extract combination.