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PREVALENSI MALARIA PADA DAERAH ENDEMIS ORANG RIMBA PROVINSI JAMBI MENGGUNAKAN PEMERIKSAAN MIKROSKOPIS Ade Suryaman; Chairil Anwar; Dwi Handayani; Sulfa Esi Warni; Irsan Saleh; Dalillah Dalillah; Gita Dwi Prasasty
Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health) Vol 4 No 1 (2020): Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health)
Publisher : Poltekkes Kemenkes Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35910/jbkm.v4i1.265

Abstract

Background: Malaria remain a world health problem with the highest number of case are in tropic and subtropic country such as Indonesia. Orang Rimba is a native tribe living secluded deep in the Taman Nasional Bukit Duabelas Forest and in the northern area were living in the Taman Nasional Bukit Tigapuluh. For demography, tteh were unregistered due to the difficulties of the survey, since they were living nomaden. Lack of transportation, living deep in the forest, low education level, bad living environment and low social economic level affect the risk of Malaria case number. This research amied to identifiy the prevalence of Malaria on endemic location of orang rimba in jambi province using microscopic examination. Methods: This was a descriptive observational study. The population in this study were all communities of Anak Rimba. A total of 94 Anak Rimba were recruited for the study.Malaria status were checked using gold standar which is Microscopical examination of the blood smear. Results: The results showed that 4,95% of the respondents were positive Malaria with plasmodium vivax (3,96%) and 0,99% special falciparum Conclusion: There were high prevalence of Malaria on Anak Rimba at Sungaiterap Desa Jelutih, Kec. Batin XXIV, Kabupaten Batangahari, Provinsi Jambi
KEPADATAN VEKTOR DAN STATUS RESISTENSI LARVA Aedes aegypti DI DESA PANCUR PUNGAH KECAMATAN MUARA DUA KABUPATEN OKUS TAHUN 2019 Dwi Handayani; Fadjar Siddiq Hidayatullah; Chairil Anwar; Sulfa Esi Warni; Dalilah Dalilah; Lasbudi P Ambarita; Gita Dwi Prasasty
JURNAL KEDOKTERAN DAN KESEHATAN Vol 7, No 2 (2020)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.267 KB) | DOI: 10.32539/JKK.V7I2.9710

Abstract

Keberhasilan pengendalian penyakit demam berdarah dengue tergantung dari status kerentanan vektor terhadap insektisida yang digunakan. Sampai saat ini dikenal dua vektor DBD yaitu Aedes aegypti sebagai vektor utama dan Aedes albopictus sebagai vektor sekunder. Tujuan penelitian ini untuk mengetahui kepadatan vektor Aedes aegypti dan status resistensinya terhadap larvasida Temephos di Desa Pancur Pungah Kabupaten Muara Dua Kabupaten Ogan Komering Ulu Selatan. Penelitian ini merupakan penelitian deskriptif observasional. Sampel pada penelitian ini adalah 100 buah rumah, tempat penampungan air, dan larva yang ditemukan di dalam tempat penampungan air. Tempat penampungan air dan larva didalamnya diidentifikasi kemudian dilakukan penghitungan dan uji larvasida dilakukan dengan menggunakan Temephos 0,02 ppm sebanyak 5 kali pengulangan. Hasil penelitian didapatkan sebanyak 389 tempat penampungan air dan 55 diantaranya terdapat larva. Hasil perhitungan menunjukkan HI: 35, CI: 14,1 dan BI: 55 dan uji larvasida menunjukkan semua larva yang dipaparkan terhadap Temephos 0,02 ppm mengalami kematian. Dari perhitungan tersebut didapatkan kepadatan vektor berada pada intensitas sedang dan status kerentanannya masih sensitive atau susceptible terhadap Temephos. Hasil penelitian ini diharapkan dapat digunakan sebagai bahan evaluasi bagi kebijakan pengendalian vektor DBD setempat.
Deteksi resistensi insektisida sintetik piretroid pada Aedes aegypti (l.) Strain palembang menggunakan teknik polymerase chain reaction Ahmad Giffari; Humairo Fatimi; Chairil Anwar
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 5 No 2 (2013): Jurnal Aspirator Volume 5 Nomor 2 2013
Publisher : Loka Litbang Kesehatan Pangandaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.037 KB)

Abstract

Aedes aegypti is a vector of several pathogens including dengue fever/dengue hemorrhagic fever virus. Five hundred thousand dengue haemorrhagic fever new cases occur every year throughout the world. Vector control is an effective way to break the transmission; unfortunately constant insecticide ultimately caused resistance. Insecticides resistance in Ae.aegypti was first discovered on trichloroetane diphenyl dichloro (DDT), followed by temephos and synthetic pyrethroid. Three detection ways according to WHO procedure are bioassay, biochemistry and molecular. The biochemical detection that conducted previously in Palembang were turned out negative, nevertheless incidence rate has not yet decreased. Molecular detection is needed to determine the mechanisms of insecticide resistance. Molecular detection can detect gene mutations in the metabolic enzyme and target site insecticides, such as the voltage gated sodium channel (VGSC). The purpose of research was to identify the Val1016Ile and Val1016Gly point mutation in the VGSC gene of Ae.aegypti in Palembang. Population were all 3rd and 4th instar larvae of Ae.aegypti derived from breeding eggs obtained from villages of Bukit Kecil, Ilir timur I and Sukarami sub distric. Identification took place in BBLK Palembang while molecular test took place both in BBLK Palembang and Clinical Microbiology Department of Muhammad Hoesin Hospital Palembang. Results showed that there has been Val1016Ile point mutation and there is no Val1016Gly point mutation of voltage gated sodium channel gene. It can be concluded that there has been Val1016Ile point mutation in the voltage gated sodium channel gene of Ae.aegypti as the marker of synthetic pyrethroid insecticides resistance in Palembang
Papaya Leaf (Carica papaya L.) Active Fraction Effective as Bioinsectiside Against Anopheles species (Diptera: Culicidae) Larva Invitro Study Chairil Anwar; Febriyanto Febriyanto; Dalilah Dalilah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 1 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i1.15

Abstract

ABSTRACT Malaria is one of the spread-disease that become a global public health problem included Indonesia. One of the controlling approach of vector-borne disease is by avoiding direct contact of human and mosquitoes. Natural insecticide is an alternative method that can be used. Carica papaya L. is one of the natural insecticide because contains of alkaloid, flavonoid and saponin. This research was aimed to determine the activity of active fraction. was a quasi-experiment research with post test only group design. This research was conducted in April – June 2017. Extraction and fractination was performed in laboratory of MIPA Biologi of Sriwijaya University. The experiment was performed in laboratory of Entomologi Baturaja. The sample of the research was Anopheles sp. larva instar III and IV. Larvacide test used 6 different concentrations which was 0,125%, 0,25%, 0,5%, 1,0% and 2,0% with aquadest as a control. Anova test and post hoc analysis with SPSS 22 software. The result of efficacy test to Anopheles sp. larva showed that N-hexan was the fraction with highest larvacide ability that kill 95% larva at 2% concentration level. Thin layer chromatography test showed a compound of Terpenoid. Activity test of active fraction N-heksan of papaya leaf with the highest mortality rate 92,5% and the lowest 46,25%. LC50 fraction N-heksan of papaya leaf was high toxicity at 24 hours of observation. Anova test showed there was significant influence and difference of various concentration of active fraction of papaya leaf to the mortality of Anopheles sp. larva with p value < α (0,00<0,05). Key words : Anopheles sp. larva, N-hexan fraction of papaya leaf, larvacide
The Relationship Between Soil Transmitted Helminthes (STH) Infection and Nutritional Status in Students of State Elementary School Number (SDN) 200 Palembang Indonesia Chairil Anwar; Saraswati Annisa; Dalilah Dalilah; Novrikasari Novrikasari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 2 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i2.39

Abstract

Infection of Soil Transmitted Helminthes (STH) is caused by intestinal nematodes where as in its life cycle, soil is needed as the media for the eggs or larvae to mature into effective forms, most commonly happen to children of school age. This infection is still one of the main problems in public health, including Indonesia. STH infection are widely distributed in tropical and subtropical areas. Lack of personal hygiene, poor environmental sanitation and low socioeconomic status are some factors that plays role in increasing the occurrence of the infection. This infection is also one of the causes responsible for malnutrition in children by decreasing appetite and food intake thus ensued adverse consequences such as declining growth pace, impairment of physical health, and weakening cognitive function. This study was conducted to analyze the association of STH infection with nutritional status of SDN 200 students in Kertapati District, Palembang. This study was an analytic observational research with a cross sectional research design. Samples consist of 107 students chosen using proportional stratified random sampling technique. Data was collected by direct interview using questionnaires, measuring body weight and height to obtain nutritional status which then classified using CDC 2000 growth curve while fecal contamination was examined using Kato Katz and modified Harada Mori methode in the Laboratory of Parasitology Medical Faculty of Universitas Sriwijaya. Data then analyzed using Chi-square test. From 107 students, 27.1% infection of STH was found on 29 students with 6 students (20.7%) infected by A. lumbricoides and 23 students (79.3%) infected by T. trichiura. Proportion of malnutrition status was found at 43.9%. Statistical test showed a significant association between STH infection and nutritional status (p=0.036; OR=3.167; Cl 95%: 1.163-15.237). There was a significant association between STH infection and nutritional status in students of SDN 200 Kelurahan Kemas Rindo Kertapati District Palembang City.
The Efficacy of Red Ginger Fraction (Zingiber officinale Roscoe var. rubrum) as Insecticidal Aedes aegypti Chairil Anwar; KM Yahya Syukur; Dalilah Dalilah; Salni Salni; Novrikasari Novrikasari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 2 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i2.40

Abstract

Aedes aegypti is a vector of Dengue Hemoragic Fever. The use of synthetic chemical insecticide is the most common way to control Ae. aegypti. However, since it can cause resistency if it is used in a wide scale with high frequency and continously, it requires an alternative way. Red ginger (Zingiber officinale Roscoe var. rubrum) is one of natural insecticide which is safe to be used to control Ae. aegypti. This research aims was to determine the activity of active fraction of red ginger to mortality of Ae. aegypti. It was a quasi-experiment research with post test only control design. Twenty Ae. aegypti Liverpool strain were the sample of this research with 5 concentration for each and 4 times repetition. The result showed that concentration 0.5%, 1.0%, 1.5%, 2.0% and 2.5% causing mortality percentage of 32.5%, 33.8%, 51.3%, 58.8% and 65.0% respectively. One way Anova test showed p-value < alpha ± (0.00 < 0.05). It was concluded that there was a significant difference of various concentration of active fraction n-heksan of red ginger extract to the mortality of Ae. aegypti. The lethal concentration (LC50) was 2.409%. It was suggested to continue this research with reppellant or bio-assay test method. Key words: Toxicity, extract, fraction, Aedes aegypti, Insecticidal
Association of Knowledge, Attitude and Practice of Cleaning Service with the Amount of Mosquito Larvae in Water Container At Bhayangkara Hospital, Palembang, Indonesia Chairil Anwar; Putri Arini; Husnil Farouk; Novrikasari Novrikasari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 2 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i2.50

Abstract

Bacgkround: Efforts in the eradication of mosquito breeding have been done, but have not shown maximum results due to lack of community participation caused by several factors, such as lack of knowledge, attitude and practice that are considered to play important role in reducing the incidence of vector-borne diseases transmitted by mosquitoes. This study aimed was to determine the relationship of knowledge, attitude and practice of cleaning services with the amount of mosquito larvae at water container around Bhayangkara Hospital Palembang. Populations in this study were 25 cleaning services in Bhayangkara Hospital Palembang. Methods: Samples in this study were all populations taken by using total sampling technique. Data included the number and types of water containers, the number and species of mosquito larvae found in the water containers, and data of knowledge, attitude and practice of cleaning services. The results were analysed using Kruskal-Wallis statistic test. Results: There were 75 water containers, 63 water containers were indoors, one of them contains larvae, and 12 water containers were outdoors, the type of two outdoors water containers that contained larvae were plastic buckets. Total larvae that found were 16 larvae of Aedes aegypti. Based on Kruskal-Wallis statistic test, there was no average difference between the number of mosquito larvae based on knowledge with p>alpha± (0.072>0.05), and there was an average difference between the number of mosquito larvae based on attitudewith p<alpha± (0.006>0.05) and practice with p<alpha± (0.019<0.05). Conclusion: There was a negative association between attitude and practice against the number of mosquito larvae, but not with knowledge.
The Influence of Knowledge, Attitudes and Behavior of Cleaning Officers with the Number of Mosquito Larvae in the Water Reservoir Punti Kayu Palembang City Aldi Alfian; Chairil Anwar; Iche Andriyani Liberty
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 3 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i3.217

Abstract

Background Mosquito was one of the animals that can transmit deadly diseases.Information about water reservoirs as their habitat as well as human knowledge,attitudes and behavior factors are considered important to help eradicate mosquito.This study aims to determine the effect of knowledge, attitudes and behavior ofcleaners on the number of mosquito larvae in water reservoirs in Palembang.Methods This research was mixed method research (quantitative and qualitative) inthe field and laboratory. The research samples were all mosquito larvae andcontainers found. The research respondents were all cleaning services. Data werecollected by interviewing and filling in questionnaires, observing water reservoirsand identifying the types of mosquito larvae in microscope. Results A total of 40containers were found in Punti Kayu Nature Park. There were 8 containers that wereindoors (20%) and 32 containers were outdoors (80%) with 17 containers containedlarvae. Of all the containers that were positive for larvae, 6 were filled with clearwater and 11 were filled with turbid water. The number of larvae found were 358larvae outdoors and 82 larvae indoor with a total of 440 mosquito larvae with thegenus Culex spp. and Aedes albopictus species. The index of mosquito larvae wereobtained FLI (Free Larva index)=66,67%, HI (House Index)=33,33%, CI (ContainerIndex)=16,67%, BI (Breteau Index)=33.33% from the 3 buildings examined. Fromthese results, it was obtained DF (density figure) or density level of 5. There were 40containers that could be habitats for Culex spp. and Aedes albopictus. ConclusionThe knowledge, attitude and behavior of the cleaning services were good. Thenumber of mosquito larvae could be influenced by the frequency of cleaning and thelack of knowledge of the cleaning services.
Relationship of Food Leftover with Covid-19 Inpatients Recovery Speed in Palembang Indonesia Devi Eryanti; Chairil Anwar; Yuanita Windusari; Ramzi Amin; Ahmad Ghiffari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 12 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i4.377

Abstract

Background : Hospital nutrition services support the patient's recovery process, which the activity is feeding. One indicator of quality in food service is food leftover. The high food waste can affect the patient's health. The high food waste will pose a risk of malnutrition in hospitalized patients. The study aimed to analyze the food leftovers in COVID-19 inpatients at the Palembang referral hospital.Methods : This cross-sectional quantitative descriptive study was with a sample of 112 patients selected by purposive sampling in February-May 2021. Data were taken using the Comstock form and then translated in the form of percent leftover food. According to the Minister of Health, the minimum leftover food is less than 25 percent.Results : The results showed that more than 25 percent of the leftover food were 51 respondents; Thirty-three respondents (54.1%) have fully recovered and 28 respondents (45.9%) still in improvement. On the other hand, the leftovers food of less than 25 percent were 51 respondents; Forty respondents (78.4%) have fully cured, and 11 people (21.6%) still in treatments. The analysis of Chi-square test results shows a p-value of 0.013, showing a significant relationship between food waste and the recovery of COVID-19 patients.Conclusion : The food waste of COVID-19 patients in referral hospitals was still relatively high. It is necessary to modify the diet menu to prevent leftovers and speed up the patient's recovery.
Contamination of Soil Transmitted Helminths (STH) Eggs in Raw Vegetables at Street Food Stalls and Restaurant in Lorok Pakjo Village, Palembang Leo Medianto Faziqin; Dalilah; Dwi Handayani; Chairil Anwar; Susilawati
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 6 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i6.397

Abstract

Background: Infection Soil Transmitted Helminths (STH) is a disease that is a problem in the world, especially in areas with a tropical climate like Indonesia. This infection can be transmitted through consumption of raw vegetables contaminated with STH. This study aims to determine whether there is contamination of STH eggs in raw vegetables at street food stalls and restaurants in Lorok Pakjo Village, Palembang. Methods: This research is a descriptive observational study. Samples in the form of fresh vegetables were collected from 18 street stalls and 14 restaurants in Lorok Pakjo Village, Palembang. Samples were examined using the sedimentation method and observed using a microscope. Results: The results obtained 80 samples consisting of 30 cabbage, 18 basil, 30 cucumber, 1 lettuce, and 1 eggplant. Of the 80 samples, there were 20 (25%) positive samples contaminated with STH eggs consisting of 8 (10%) cabbage samples, 8 (10%) basil samples, 4 (5%) cucumber samples, and no STH contamination was found in lettuce. and eggplant. Of the 20 positive samples of STH contamination, 12 samples came from street vendors consisting of 5 (10.6%) cabbage samples, 6 (12.8%) basil samples, and 1 (2.1%) cucumber samples. While the other 8 samples came from restaurants consisting of 3 (9.1%) cabbage samples, 2 (6%) basil samples, and 3 (9.1%) cucumber samples. Found only eggs of Ascaris lumbricoides infertile decorticate in all samples consisting of 9 eggs on cabbage, 16 eggs on basil, and five eggs on cucumber. Conclusion: There is contamination of eggs Soil Transmitted Helminths in raw vegetables in street food stalls and restaurants in Lorok Pakjo Village, Palembang.