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Dua Spesies Cacing Soil Transmitted Helminths pada Sayuran Selada (Lactuca sativa) Yang Dijual di Warung Makan pada Kecamatan Seberang Ulu II Palembang Prameswarie, Thia; Chairil, Ahmad Ghiffari; Prameswari, Meta
Sriwijaya Journal of Medicine Vol. 2 No. 3 (2019): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (980.074 KB)

Abstract

Soil-Transmitted Helminths (STH) is a round intestinal worm whose life cycle requires soil. Ascaris lumbricoides, Trichuris trichiura, hookworm sp and the prevalence in Indonesia currently range from 20-86%. Transmission of disease usually food-borne and un-hygienic behaviour. The purpose of this study was to identify the contamination of Soil-Transmitted Helminths (STH) eggs in lettuce (Lactuca sativa). This research has been conducted in Seberang Ulu II Palembang District in 2018. The research method was descriptive cross-sectional with laboratory tests using 0.2% NaOH and then examined under a microscope in June 2018 until January 2019. The sample in this study were 372 lettuce leaves from 31 food stalls, each of the food stalls is taken 12 lettuce leaves. The results were 9 eggs Ascaris lumbricoides (29%), 2 Hookworm sp eggs (6%), but not the Trichuris trichiura eggs. It conclued that there are two species, Ascaris lumbricoides and hookworms sp., of egg worms on lettuce fresh vegetables (Lactuca sativa). The distribution of STH egg positivity is classified as medium in the food stall.
PENGARUH BEDA TEMPAT PELETAKKAN BANGKAI DENGAN PERTUMBUHAN LARVA LALAT PADA TIKUS Rattus norvegicus Switha, Elfinchia Tiara; Anwar, Chairil; Dalilah, Dalilah; Ghiffari, Ahmad
Syifa'Medika Vol 10, No 1 (2019): Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan
Publisher : Faculty of Medicine

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/sm.v10i1.1743

Abstract

Lalat merupakan serangga yang sering digunakan pada bidang entomologi forensik sebagai indikator penentu lama waktu kematian makhluk hidup (post mortem interval) karena merupakan serangga yang pertama kali datang pada proses dekomposisi bangkai. Tujuan penelitian adalah untuk mengetahui pengaruh berbagai tempat meletakkan bangkai terhadap pertumbuhan larva lalat pada bangkai tikus Rattus norvegicus. Sampel menggunakan 28 ekor tikus R.norvegicus yang kemudian diletakkan masing-masing di ruang terbuka, di dalam kotak kayu, di dalam tas yang dimasukkan ke dalam kotak kayu, dan di dalam kotak kayu yang ditanam di tanah. Larva yang muncul kemudian diambil dan diperiksa secara mikroskopis dengan menggunakan kunci identifikasi. Pengambilan data dilakukan selama 6 hari berturut-turut. Spesies yang banyak ditemukan adalah Chrysomya megacephala, muncul sejak hari pertama setelah kematian pada bangkai tikus.  Pertumbuhan panjang larva lalat dianalisis untuk mengetahui hubungan dengan lama kematian. Disimpulkan terdapat pengaruh tempat peletakkan bangkai terhadap pertumbuhan larva lalat pada bangkai tikus Rattus norvegicus, dan disarankan untuk melakukan perbandingan hasil serupa dengan sampel korban manusia yang ditemukan belatung.
The Duration of Reading the Qur'an with the Stress Levels in Medical Faculty Students in Palembang Ghiffari, Ahmad; Muhammad, Syahrul; Ababil, M. Avif
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 21, No 1: January 2021
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v21i1.8488

Abstract

The level of stress in each education programs differ between medical students and other scholars. Students should able to cope to normalize their stress. The study aims to determine the relationship between the duration of reading the Qur'an with the level of stress on students. The study design was a cross-sectional study, with a simple random sampling. The collecting data technique was using the Depression Anxiety Stress Scale (DASS) questionnaires and duration questionnaires followed by the chi-square analysis. The respondents are 90 medical students of Universitas Muhammadiyah Palembang. The results showed that the duration of reading the Qur'an among students is poor, and the moderate duration is correlated with a good stress level (p=0,002). Concluded that there is a relation to the duration of reading The Qur'an with the level of stress in medical students.
Performance of CO1 and ITS2 nested PCR in molecular identification of ordinary scabies (Sarcoptes scabiei var. hominis) Gita Dwi Prasasty; Miftahurrizqiyah Miftahurrizqiyah; Chairil Anwar; Dwi Handayani; Dalilah Dalilah; Ahmad Ghiffari; Inda Astri Aryani; Nunuk Dyah Retno Lastuti; Afiat Berbudi
Indonesian Journal of Biotechnology Vol 26, No 4 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.64472

Abstract

Scabies is a global disease with a high prevalence, causing morbidity and even mortality, especially in poor and developing countries. However, it is often misdiagnosed due to varied and unspecified lesions. The gold standard technique for diagnosis is a microscopic examination, which requires experienced experts in finding mites, mainly in ordinary scabies. CO1 and ITS2 genes have been widely used in molecular identification to detect Sarcoptes scabiei and its variants. This study aimed to determine and compare the sensitivity and specificity of CO1 and ITS2 S. scabiei genes to the microscopic examination of scabies skin scrapings. The skin scrapings of 52 subjects with scabies diagnosed by anamnesis, physical examination, and dermoscopic examination were examined under a microscope and analyzed by nested PCR. The diagnostic test result showed that the sensitivity of nested PCR of both CO1 and ITS2 genes to micro‐ scope examination was 100%. However, the specificity of both CO1 and ITS2 nested PCR was poor (24% and 0%). Hence, CO1 and ITS2 nested PCR could be more suitable for screening ordinary scabies in humans than the microscopic examination.
The Duration of Reading the Qur'an with the Stress Levels in Medical Faculty Students in Palembang Ahmad Ghiffari; Syahrul Muhammad; M. Avif Ababil
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 21, No 1 (2021): January
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v21i1.8488

Abstract

The level of stress in each education programs differ between medical students and other scholars. Students should able to cope to normalize their stress. The study aims to determine the relationship between the duration of reading the Qur'an with the level of stress on students. The study design was a cross-sectional study, with a simple random sampling. The collecting data technique was using the Depression Anxiety Stress Scale (DASS) questionnaires and duration questionnaires followed by the chi-square analysis. The respondents are 90 medical students of Universitas Muhammadiyah Palembang. The results showed that the duration of reading the Qur'an among students is poor, and the moderate duration is correlated with a good stress level (p=0,002). Concluded that there is a relation to the duration of reading The Qur'an with the level of stress in medical students.
PERILAKU SANTRIWAN YANG BARU MONDOK DI PESANTREN K-K KECAMATAN SAKO TERHADAP KEJADIAN PENYAKIT KULIT SKABIES Ahmad Ghiffari; Lucille Annisa Suardin; Raden Pamudji; Zadi Oktariansyah
SPIRAKEL Vol 12 No 2 (2020)
Publisher : Balai Penelitian dan Pengembangan Kesehatan Baturaja

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/spirakel.v12i2.2704

Abstract

Sekitar 300 juta kasus skabies per tahunnya dilaporkan di seluruh dunia dan di Indonesia 3,9-9% penduduk. Faktor-faktor yang berhubungan dengan risiko infestasi tungau antara lain higienitas pribadi yang buruk, kontak dengan penderita, kelembapan, kepadatan hunian yang tinggi dan pengetahuan yang rendah. Faktor risiko tersebut tinggi pada santri penghuni asrama pesantren. Kegiatan penelitian bertujuan untuk menentukan faktor risiko dan hubungannya penyakit kulit kudis pada Pondok Pesantren K-K di Kecamatan Sako Kota Palembang. Penelitian dilakukan melalui metode observasi dengan menggunakan pendekatan desain potong lintang pada 114 santri dengan prosedur pemeriksaan klinis (cardinal sign) gejala skabies dan mikroskopis serta kuesioner. Hasil menunjukkan bahwa subjek penelitian yang terkena skabies sebanyak 37% didominasi oleh jenis kelamin laki-laki (53%), berada pada tingkat tsanawiyah (63%) kelas VII (40%), memiliki tingkat pengetahuan sedang (49%), tingkat sikap sedang (48%), tingkat perilaku baik (52%). Analisis bivariat dan logistik regresi multivariat menunjukkan hubungan antara kejadian skabies dengan pengetahuan, sikap, perilaku, dengan pengaruh paling besar pada perilaku (OR 8,24). Disarankan untuk tidak menggunakan barang pribadi secara bersama. Penyuluhan terutama pada santri yang baru masuk asrama harus lebih digalakkan dengan melibatkan pihak puskesmas untuk sosialisasi penyakit terutama bagi santriwan kelas VII tsanawiyah.
The Dominant Factors of High Scabies Incidence In Indralaya Islamic Boarding School Students South Sumatera, Indonesia Salvari Gusti Ayu Dwi Putri; Ahmad Ghiffari; Chairil Anwar; Rico Januar Sitorus
Majalah Kedokteran Sriwijaya Vol 51, No 4 (2019): Majalah Kedokteran Sriwijaya
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/mks.v51i4.10237

Abstract

Scabies is a skin disease caused by infestation towards Sarcoptes scabiei, the condition locally known as "kudis", occurring easily in moist and densely populated vicinities. Kudis was classified into one of the top 10 most prevalent diseases of Ogan Ilir District in 2014. This research was conducted to analyse the relation between air quality and personal hygiene towards the incidences of scabies among students of boarding school Al Ittifaqiah, Indralaya. This research was an epidemiological method using cross-sectional analytic approach, conducted in March 2016. The population were all students of boarding school (1.859 individuals) and the samples selected were 200 respondents with proportional stratified random sampling. The measuring instruments were cardinal scabies physical diagnostic and questionnaire of demographic interviewing, and air quality observation. Obtained results were then analysed with Chi-Square, Logistic Regression Statistical Tests, and correspondence analysis. The result of scabies prevalence was 74.0%. The statistical test has shown that all free variables present relation towards the occurrence of scabies with the p-value < 0.05 except for the variables of temperature and humidity. Logistic Regression testing result yielded the free variable bearing the most dominant influence towards scabies incidence, which was the residential density (OR: 8.117; 95% CI: 3.176-20.745), ventilation (6.712; 1.323-34.066) and age (3.246; 1.555-6.775). The significant relationship of residential density, ventilation, and age towards the occurrence of scabies should be further investigated in order to prepare for elimination programs. 
Activity of Cytochrome P450 Monooxygenase (CYPs) Metabolic Enzymes as Markers of Insecticide Resistance in Anopheles vagus Muara Enim Mosquitoes, Indonesia Dalilah; Muhaimin Ramdja; Dwi Handayani; Lasbudi P Ambarita; R. Irpan Pahlepi; Ahmad Ghiffari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 4 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i4.222

Abstract

The use of synthetic pyrethroids in insecticide-treated bed nets (LLINs) and spray form (IRS) has been carried out since 2012 in Muara Emil Village and since 2016 in all villages in Tanjung Agung District, Muara Enim Regency. Biochemical resistance can occur to the enzymes that play a role in detoxifying the insecticide. This study aims to identify whether there is an increase in the levels of the enzyme Cytochrome P450 monooxygenase (CYPs) through the enzyme biochemical test on the malaria vector mosquito Anopheles vagus originating from Pagar Dewa Village and Muara Emil Village, Muara Enim Regency. The sample of female Anopheles mosquitoes was not full. Blood was taken at night using the resting collection method. Mosquitoes that have been identified as Anopheles vagus species are used to check CYPs enzyme levels using the ELISA method. The results of the biochemical test showed that enzyme levels increased from the Muara emil and Pagar Dewa village mosquitoes with a percentage of 71% and 61.53% (with cut off point OD> 0.165). The value of CYPs enzyme levels for mosquitoes from Muara Emil village was higher than that of the mosquitoes from Pagar Dewa Village (mean 0.005027 ± 0.007). The increased activity of the CYPs enzyme plays a role in the detoxification of synthetic pyrethroid insecticides that can cause resistance. The high levels of enzymes from the villages of Muara Emil and Desa Farah Dewa are in line with intensive insecticide exposure in eradicating malaria vectors in the highest malaria transmission areas in Muara Enim Regency.
Philogenetic Analysis of S. Scabiei Var. Hominis, Var. Cuniculi, and Var. Marmoota Gita Dwi Prasasty; Miftahurrizqiyah; Dalilah; Dwi Handayani; Tia Sabrina; Ahmad Ghiffari; Inda Astri Aryani
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 10 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i10.352

Abstract

Introduction: Based on data from the 2017 Global Burden of Disease, it is known that Indonesia is the country with the highest number of scabies incidences out of 195 countries in the world. In addition to humans, scabies is also an infectious disease in mammals with a prevalence of 300 million infestations every year, even in animals the mortality rate from scabies is very high. Until now, it is still unclear whether there is a cross-infestation between human and animal scabies. Morphologically, S. scabiei between variants cannot be distinguished, while research on genetic variation is still not much done. Therefore, it is necessary to conduct studies to enrich genetic information and phylogenetic analysis. Methods: The samples tested were positive PCR results for ITS 2 and CO 1 genes, each on five amplicons, namely three S. scabiei var. hominis, one var. cuniculi, and one var. marmoota. Phylogenetics were arranged based on the closeness of the base arrangement in variants and geographically available in NCBI. Results: Of the five sample isolates studied, the ITS 2 gene amplicon is located in the 417bp band and the phylogenetic analysis of its sequencing has two branches that are unable to distinguish var. hominis and var. animalia. The CO1 gene has electrophoretic results at 317 bp as well as phylogenetic analysis results that specifically divide branches between var. hominis and var. animalia. Conclusion: S. scabiei var. hominis and var. marmoota, and var. cuniculi are distinct species and are host monospecific.
Relationship of Food Leftover with Covid-19 Inpatients Recovery Speed in Palembang Indonesia Devi Eryanti; Chairil Anwar; Yuanita Windusari; Ramzi Amin; Ahmad Ghiffari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 12 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i4.377

Abstract

Background : Hospital nutrition services support the patient's recovery process, which the activity is feeding. One indicator of quality in food service is food leftover. The high food waste can affect the patient's health. The high food waste will pose a risk of malnutrition in hospitalized patients. The study aimed to analyze the food leftovers in COVID-19 inpatients at the Palembang referral hospital.Methods : This cross-sectional quantitative descriptive study was with a sample of 112 patients selected by purposive sampling in February-May 2021. Data were taken using the Comstock form and then translated in the form of percent leftover food. According to the Minister of Health, the minimum leftover food is less than 25 percent.Results : The results showed that more than 25 percent of the leftover food were 51 respondents; Thirty-three respondents (54.1%) have fully recovered and 28 respondents (45.9%) still in improvement. On the other hand, the leftovers food of less than 25 percent were 51 respondents; Forty respondents (78.4%) have fully cured, and 11 people (21.6%) still in treatments. The analysis of Chi-square test results shows a p-value of 0.013, showing a significant relationship between food waste and the recovery of COVID-19 patients.Conclusion : The food waste of COVID-19 patients in referral hospitals was still relatively high. It is necessary to modify the diet menu to prevent leftovers and speed up the patient's recovery.
Co-Authors Ababil, M. Avif Adelia Yulantasya Adelia, Karlita Afiat Berbudi Alpasyah, M. Mico Amira Azza Nabila Andelina, Rizky Anggina, Dientyah Nur Anggina, Dientyah Nur Anisa Suardin, Lucille Antoni Antoni Anwar, Chairil Apri Wahyudi Apriko Apriko Ardi Artanto Ardi Artanto Ardina, Puri Pratami Artanto, Ardi Asmalia, Resy Asysyauki, Akhmad Hamdi Atika Safitri Armo Azhara, Alya Badri, Putri Rizki Amalia Bagus Setiawan, Rifqi Budi Utama Budi Utama, Budi Chairani, Liza Chairil Anwar Chairil Anwar Chairil Anwar Chairil Anwar Chairil Anwar Chairil Anwar Charil Anwar Dalilah Dalilah Debby Andhika Putri Defi Mai Rani Delfi Riana, Siska Deris Stiawan Devi Eryanti Dewi Yuniasih Didik Agus Prawira Dientya Nur Anggina Dina Mulyana Dwi Handayani Dwi Handayani Dwitania, Tasya Julia Edi Surya Negara Eni Nuraini Faradila, Faradila Fatihah, Nabila Nurul fatimah Fatimah Fatinah Fairuz Qonitah Febri Putri Lintang Sari Fika Nurani Siti Aisyah Galuh Ismayanti Gita Dwi Prasasty Gumar Herudiansyah Gusmiatun Gusmiatun Haerawati Idris Hafidz, Ridho Hakim, Aibi Hamzah Hasyim Haryani Haryanto, Didid Helwan Kasra Helwan Kasra Heni Nurdita Herudiansyah, Gumar Hilda Zulkifli I.E Habibi, Nur Ikbar, Iffat Nabila Inda Astri Aryani Indah Purnama Sari Indriyani Indriyani Irzal, Feirully Iskandar Z. A Ismail Ismail Ivanka, Agnes Jamhari Jamhari Jhon Riswanda Julia Liona Putria, Sinta Kasra, Helwan Keti Sonia Khoirotun Niswa Kurnia, Aisyah Fadhilah Kurniatullah, Ridho Lasbudi P Ambarita Latius Hermawan Liza Chairani Liza Chairani Lucille Anisa Suardin Lucille Annisa Suardin M. Abidinsyah M. Avif Ababil Maka Suci, Tassya Mayasari, Ni Made Elva Meilinda Meilinda Melisa Nopa Belia Meta Prameswari Miftahurrizqiyah Miftahurrizqiyah Miftahurrizqiyah Miranti Dwi Hartanti Mitayani Purwoko, Mitayani Mohammad Zulkarnain Muchsiri, Mukhtarudin Muhaimin Ramdja MUHAMMAD FAHMI Muhammad Ihsan Muhammad Qurhanul Rizqie Muhammad Satria Alfit Muhammad Wahyu Hidayat, Muhammad Wahyu Mukhtarudin, Kuntafie Al Haq Mynanda, Galuh Pratama Noviyanti Noviyanti, Noviyanti Nunuk Dyah Retno Lastuti Nur Alam Fajar Nurani, Diva Nurfauza, Aqila Meisya Putri Mulya Nurul Qamariah, Nurul Oktariza, Rury Tiara Otchi Putri Wijaya Pamudji, Raden Pamudji, Raden Permana, Adhi Prameswari, Meta Prameswari, Meta Prameswarie, Thia Prameswarie, Thia Prasasti, Gita Dwi Prayoga, Randi Putri Erlyn R. Irpan Pahlepi Raffi Ondri, Muhammad Ramayanti, Indri Ramzi Amin, Ramzi Ratika Febriani Resy Asmalia Reza Al Fath Reza Reza Rico Januar Sitorus Rico Januar Sitorus Rico Januar Sitorus Risdiansyah Risdiansyah Rizma Adlia Syakurah Rizma Adlia Syakurah Ronald Parich Riat Pratama Rosita, Yanti Rusdi, Fadhillah Putri Safitri, Rizka Nabila Salvari Gusti Ayu Dwi Putri Sandi, Louwen Saputra, Amja Sepria Sofyan, Dea Septari, Nanda Shalshabilla, Alysha Titania Silvana, Rista Silvana, Rista Silvia Rahmi Sinta Nabila Siska Fitriani Siti Nurjannah Soni Parengga Sri Wiyani Suarni, Ertati Sulton Nawawi Switha, Elfinchia Tiara Syahrul Muhammad Syahrul Muhammad Tanzila, Raden Ayu Tasya Aulia Dita Thia Prameswarie Thia Prameswarie Tia Fhadillah, Rafika Tia Sabrina Toha, Sidik Nur Tusadiah, Halimah Widiya Yolanda Wieke Anggraini Wildan, Sahrul Wilma Hannie Daniel Wulandari Saputri, Wulandari Yanti, Darma Yogi Saputra, Yogi Yolanda Olivia Yuanita Windusari Yurni Fadhillah Zadi Oktariansyah Zalika, Putri