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Journal : REKAYASA

Analisis Environmental-DNA (E-DNA) Untuk Estimasi Jumlah Total Bakteri Pada Air Kolam dengan Sistem Recirculation Aquaculture System (RAS Kurniawan, Andi; Amin, Abd. Aziz; Guntur, Guntur; Susanti, Yogita Ayu Dwi; Wiryawan, Adam; Pramudia, Zulkisam; Okuda, Hideki; Yanuar, Adi Tiya
Rekayasa Vol 15, No 3: Desember 2022
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/rekayasa.v15i3.16844

Abstract

Catfish is an aquaculture commodity that has great potential to be developed in various regions in Indonesia. The success of catfish cultivation is primarily determined by creating a suitable environment for catfish. One way to create a suitable environment for catfish cultivation is the Recirculation Aquaculture System (RAS). In applying RAS, the presence of organic matter in the pond is controlled so the pond water can be reused. The presence of organic matter in pond water is determined mainly by the activity of bacteria in the water. These bacteria are actively involved in the pond's residual organic matter degradation process. Therefore, the total number of bacteria in the water can be one of the essential parameters in the RAS. The method widely used to calculate the abundance of bacteria is counting the number of bacteria based on bacterial culture method. However, the bacteria that can be cultured are only a small part of the bacteria in the water, so the abundance value obtained does not reflect the actual bacterial population. Therefore, it is necessary to analyze the abundance of bacteria using a method closer to the actual abundance value, such as the environmental DNA (eDNA) analysis. This study aims to analyze the abundance of bacteria in water in catfish aquaculture with RAS using the eDNA method. In addition, this study also analyzes water quality data (temperature, pH, Dissolved Oxygen). This study's results indicate that the water temperature value in catfish ponds ranges from 28.0 to 29.0 °C, the average pH value is 7.7, and the dissolved oxygen is between 5.7 - 6.2 mg/L. The water quality analysis results indicate that RAS can maintain optimum conditions in the catfish cultivation process. This study also showed that the total microbial abundance value at the beginning of cultivation was 1.68 × 107 cells/ml, and on day 30, it was 3.6 × 106 cells/ml. The dynamic of bacterial densities in this study may indicate that this system can maintain the stability of the microbial community. 
Aplikasi Tandon-Filter untuk meningkatkan Kualitas dan Kuantitas Garam dalam Teknologi Greenhouse Salt Tunnel di Pantai Selatan dan Utara Jawa Timur Amin, Abd. Aziz; Salamah, Lutfi Nimatus; Zamzami, Ilham Misbakudin Al; Susanti, Yogita Ayu Dwi; Yanuar, Adi Tiya; Kurniawan, Andi
Rekayasa Vol 17, No 1: April, 2024
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/rekayasa.v17i1.24877

Abstract

Salt is an essential commodity that concerns the livelihood of society and cannot be substituted by other commodities. East Java is the largest salt-producing center in Indonesia. The results of community salt production often still have NaCl content 95%. To achieve salt production results with NaCl quality 96%, research, and technology development are needed to eliminate impurities and improve the quality of the salt produced. One of the technologies being developed is the utilization of reservoirs and raw water filters. This research aims to analyze the application of reservoir and filter technology in improving the quality and quantity of salt production on the South and North Coasts of East Java using greenhouse salt tunnels. The results of this study indicate that the dynamics of environmental parameters and the quality of raw water on the South and North Coasts of East Java affect the results of the salt production process. The quality and quantity tests of salt with reservoir and filter application on the South Coast of East Java show NaCl quality of 98.13%, water content of 5.44%, and whiteness presentation value of salt 80.5% with production quantity of 7 kg/m². In trials on the North Coast of East Java, the salt quality was 98.25%, water content was 5.37%, and the whiteness percentage value of salt was 79%, with a salt production quantity of 7.8%. This research indicates that reservoir and filter technology can improve the quality and quantity of salt according to the characteristics of the South Coast and North Coast of East Java.