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Journal : Bionature

Antibiotic Sensitivity Testing of Bacteria Isolated from Patients Suspected of Urinary Tract Infection: Uji Sensitivitas Antibiotik terhadap Bakteri yang Diisolasi dari Pasien Diduga Infeksi Saluran Kemih Erpi Nurdin; Suanbani, Dawika; Mukhtasyam Zuchrullah
Bionature Vol. 25 No. 1 (2024): April-Juni 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i1.1883

Abstract

Urinary tract infections (UTIs) is a condition wherein inflammation occurs, caused by the proliferation of microorganisms in the human urinary tract. Antibiotics are the main choice in treating UTIs, the aim of giving antibiotics is to treat acute infections and prevent urosepsis. However, deviant use of antibiotics will reduce the sensitivity of antibiotics to bacteria. The aim of this study was to determine the sensitivity of bacteria to antibiotics from urine specimens of patients with suspected urinary tract infections (UTIs). The examination begins with the determination of the bacterial species and continues with the sensitivity test of the Kirby-Bauer diffusion method, looking at the barrier zone of each antibiotic. The observation results showed that there were 6 types of bacteria found from the urine culture results of patients with suspected urinary tract infections, with the most types of bacteria being gram positive S. aureus in 13 cases (40%), gram negative E. coli in 6 cases (18%), K. oxytoca and E. aerogenes were 5 cases (15%). According to the results of the antibiotic sensitivity test, each one shows the percentage of sensitive antibiotics. Ciprofloxacin is 100% against E. coli, K. oxytoca, P. mirabilis, 60% against E. aerogenes and 46% against S. aureus. Gentamicin is 100% against E. coli, K. oxytoca, P. mirabilis. Ampicillin is 83% against E. coli, 60% against S. epidermidis. Amoxicillin is 66% against E. coli. In conclusion, based on test results, Ciprofloxacin has better sensitivity, followed by gentamicin, ampicillin and amoxicillin. Keywords: antibiotics, sensitivity testing, urinary tract infection
Observation of Candida spp. in the urine of Female Teens : Observation of Candida spp. in the urine of Female Teens Nurdin, Erpi; Mukhtasyam Zuchrullah; Nofita Sari Djurumudi
Bionature Vol. 25 No. 2 (2024): Oktober-Desember 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i2.4529

Abstract

Candida albicans is an opportunistic pathogenic fungus that most often causes urinary tract infections. The presence of C. albicans and NCA species in urine is known as candiduria, which can cause urinary tract infections. The presence of C. albicans in urine is caused because the female urethra is shorter than that of men. Research purposes to determine the frequency distribution and presentation of the presence of Candida spp. in the urine of female adolescents at the Hidayatullah Islamic Boarding School in Ternate City. Method this study uses a descriptive research type. This study describes the presence or absence of Candida spp. in the urine of female adolescents at the Hidayatullah Islamic Boarding School in Ternate City. Research results show obtained 40 urine samples of female adolescents examined, it was found that 14 samples (35%) were positive for C. albicans and 26 samples (65%) were negative for C. albicans. Results of this study concluded that 35% of the urine of female adolescents at the Hidayatullah Islamic Boarding School in Ternate City was infected with C. albicans. Keywords: Candida albicans, candiduria, urinary tract infection
Pewarnaan Alternatif dengan Menggunakan Filtrat Kulit Kenari pada Uji Mikroskopik Jamur Candida Albicans dan Aspergillus Niger Erpi Nurdin
Bionature Vol. 23 No. 1 (2022): April 2022
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Abstract

Kenari merupakan tanaman asli Indonesia yang banyak tumbuh didaerah Indonesia bagian timur, seperti Sulawesi Utara, Maluku dan pulau Seram.Kulit kenari mengandung senyawa fenolik yang merupakan antioksida alami.Senyawa fenolik dipakai sebagai zat pengawet karena fenol dapat menangkapradikal bebas. Warna yang di kandung oleh kulit kenari memungkinkan sebagaialternatif pada pewarnaan uji mikroskopik jamur. Penelitian ini bertujuan Untukmelihat jamur pada pewarnaan alternatif kulit kenari dengan menggunakan ujimikroskopik. Metode yang digunakan dalam penelitian ini yaitu deskriptifdengan pendekatan eksperimen. Hasil penelitian diidentifikasi sebanyak 3sampel (100%) Candida albicans dan 3 sampel (100%) Aspergillus niger padafiltrat dengan perlakuan pemanasan, begitu pula 3 sampel (100%) Candidaalbicans dan 3 sampel (100%) Aspergillus niger pada filtrat dengan perlakukantanpa pemanasan. Pengamatan mikroskopik jamur Candida albicans danAspergillus niger, tampak lebih jelas dan terwarnai pada penggunaan filtratpewarnaan aternatif filtrat kulit kenari dengan proses pemasan. Kesimpulan :Pewarnaan alternatif dengan filtrat kulit kenari dapat di gunakan dalammewarnai jamur Candida albicans dan Aspergillus niger. Filtrat denganpemanasan lebih efektif pada uji mikroskopik jamur.
Studi Tuberkulosis Kontak Serumah pada Masyarakat Pesisir di Wilayah Kerja Puskesmas Siko Kota Ternate Erpi Nurdin; Irma B. Hi. Lewa; Mukhtasyam Zuchrullah
Bionature Vol. 23 No. 2 (2022): Oktober 2022
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Abstract

Pulmonary tuberculosis is still the main cause of death in the world, accounting for 2 million people die every year. WHO states that around 1.9 billion people, one third of the world's population, have been infected with tuberculosis germs, every second one person is infected with tuberculosis in the world. Pulmonary tuberculosis is transmitted through droplet spread with the source of infection being patients who are coughing with household contacts as a high risk group for transmission. To find out the results of the examination and see the percentage of positive or negative acid-fast bacilli (AFB) in a sample of clients who have a history of household contact with patients with pulmonary tuberculosis. This study uses a with correlation approach research method with an observational approach. The sampling technique is purposive sampling, namely sampling based on the considerations of the researcher. The examination method used was the Zielh Nelseen method of 30 sputum samples examined. From 30 respondents, 4 respondents were found positive for microscopic acid-fast bacilli (AFB). 2 positive family members, have the characteristics of dense housing (not eligible), humidity and ventilation do not meet the requirements, but the type of floor still meets the requirements. Based on the research that has been carried out, it is concluded that 4 (13%) respondents were identified as positive for scanty with the presence of tuberculosis in 3 places of tuberculosis in household contacts, with factors that influence the occurrence of tuberculosis in household contacts. There is no significant relationship between the results of AFB examination and gender (p value = 0.407> 0.05). There is no significant relationship between the results of AFB examination and age (p value = 0.272> 0.05).