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All Journal Jurnal SOLMA Jurnal Analis Kesehatan Jurnal Mitra Kesehatan Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Pharmaqueous Jurnal Laboratorium Khatulistiwa
Nugroho, Yusuf Eko
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Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Other

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The Development of A Mini Autoclave for Bubur Ceria MPASI Products as an Alternative to Prevent Growth Retardation Faizal, Imam Agus; Nugroho, Yusuf Eko; Yunadi, Frisca Dewi
Jurnal SOLMA Vol. 12 No. 3 (2023)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v12i3.12810

Abstract

Background: Prinsip hygiene dan sanitasi makanan merupakan bentuk upaya pencegahan penyakit pada manusia. Mitra pengabdian ini yaitu “Bubur Bayi Sehat Ceria” yang sudah banyak berkontribusi dalam pencegahan stunting sebagai MPASI. Tujuan Pemberdayaan Masyarakat Pemula (PMP) pengabdian ini yaitu menginovasi pengemasan bubur dari cup plastic ke autoclavable pouch agar bubur lebih awet dan tahan lama dan menerapan teknologi berbasis mini autoklaf saat bubur dikemas agar lebih higienis dan steril terhadap patogen. Metode: Pelaksananaan PMP dimulai survei awal dan akad kerjasama mitra dengan pihak mitra kerjasama dengan Metode Focus Group Discussion (FGD). Sosialisasi materi dan Pelatihan di Audotorium dan Laboratatorium Mikrobiologi Universitas Al-Irsyad Cilacap. Evaluasi pelaksanaan dan keberlanjutan kegiatan PMP. Hasil: Terlaksana sosialisasi materi dan pelatihan pengemasan bubur ceria dengan teknologi autoclavable pouch. Sebanyak 30% setuju dan 70% memilih setuju sekali terkait penilaian kemasan dan leaflet baru bubur ceria. Kesimpulan: Pengemasan bubur dengan autoclavable pouch menjadikan bubur lebih higienis steril, dan tahan lama.
In Vitro Anti-Biofilm Activity of Nutmeg (Myristica fragrans) Leaf Infusion Extract Wijayanti, Faulia Triana; Nugroho, Yusuf Eko; Pangesti, Ira
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2097

Abstract

Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium commonly associated with severe infections, particularly in immunocompromised individuals. One of its major virulence factors is biofilm formation, which contributes to increased antimicrobial resistance. This study aimed to evaluate the inhibitory activity of nutmeg (Myristica fragrans) leaf infusion extract against P. aeruginosa biofilm formation in vitro. The experiment was conducted using a 96-well microtiter plate assay, and biofilm intensity was quantified at 595 nm following crystal violet staining. The results demonstrated that the nutmeg leaf infusion extract significantly inhibited biofilm formation, with varying levels of effectiveness depending on incubation time. These findings indicate the potential of nutmeg leaf infusion extract as an antibiofilm agent against P. aeruginosa.
Sensitivity of Torch Ginger and Basil Extracts Against Candida sp. from Vaginal Discharge Samples Arelliza, Fatma Sheilla; Nugroho, Yusuf Eko; Faizal, Imam Agus
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2100

Abstract

Pathological vaginal discharge is a common infection among women, often caused by the fungal species Candida albicans. The increasing resistance to synthetic antifungal agents such as ketoconazole has prompted the need for alternative herbal therapies. This study aimed to identify the presence of Candida albicans in vaginal swab samples from female D4 TLM UNAIC students an to assess the antifungal activity of combined extracts of kecombrang flower (Etlingera elatior) and basil leaves (Ocimum basilicum) prepared using the infusion method. An experimental design was used with the well diffusion method to measure inhibition zones at extract concentration of 50%, 70%, and 90%. The results showed that 31% of the 48 samples tested positive for Candida albicans. Macroscopically, the colonies appeared white to cream, convex, and had a characteristic yeast odor, while microscopic examination revealed blastospores. The positive control group using ketoconazole exhibited strong antifungal activity with an inhibition zone diameter of 23 mm. In contrast, the combined infusion extracts at all tested concentration (50%, 70%, and 90%) showed no inhibition zones (0 mm), indicating an absence of antifungal activity. The conclusions of this research are the infusion extraction method was ineffective in isolating active antifungal compounds from the tested plants under the conditions of this study. 
CORRELATION OF HBA1C LEVELS OF PROLANIS PATIENTS WITH DIABETES MELLITUS TO MICROALBUMINURIA LEVELS AT KESUGIHAN I HEALTH CENTER Nugrahaeni, Dian Rahma; Faizal, Imam Agus; Nugroho, Yusuf Eko
Jurnal Mitra Kesehatan Vol. 8 No. 1 (2025): Jurnal Mitra Kesehatan
Publisher : STIKes Mitra Keluarga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47522/jmk.v8i1.422

Abstract

Introduction : Chronic hyperglycemia and disorders of carbohydrate, fat, and protein metabolism are signs of a metabolic disease known as diabetes mellitus. The disease is caused by abnormalities in the secretion of insulin or the work of insulin. Higher HbA1C levels indicate uncontrolled diabetes mellitus, and in high-risk patients, long-term complications that can be fatal. The presence of albumin in the urine of people with diabetes mellitus causes a problem known as diabetic nephropathy. In a damaged glomerular condition, kidney leakage can occur which allows proteins, especially albumin, to seep into the urine. The purpose of this study was to find out whether there was a relationship between HbA1C levels of prolanis diabetic mellitus patients and microalbuminuria levels at the Kesugihan I Health Center. Method: This study used a cross-sectional description design, with a research sample of results from the HbA1C and microalbuminuria examination of prolanis patients at the Kesugihan I Health Center from January to March 2025, meeting the inclusion and exclusion criteria of 60 samples. Result: The Spearman correlation test for this study found a correlation value of -0.163 between HbA1C and microalbuminuria levels, with a p-value of 0.214. Conclusion: The conclusion of this study is that there is no correlation between HbA1C levels of prolanis patients with diabetes mellitus and microalbuminuria levels at the Kesugihan I Health Center.
Analisis Profil Protein dan Total Protein Tempe Mendoan Asal Cilacap Dengan Berbagai Durasi Fermentasi Menggunakan SDS-PAGE Putri, Reza Amelia; Farabi, Meka Faizal; Nugroho, Yusuf Eko
Jurnal Analis Kesehatan Vol. 14 No. 2 (2025): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v14i2.5169

Abstract

Di Indonesia tanaman kedelai (Glycine max (L) Merill) banyak dibudidayakan, tetapi bukan tanaman asli Indonesia. Salah satu produk olahan kedelai yang gemar dikonsumsi oleh masyarakat adalah tempe, merupakan makanan tradisional dari Indonesia. Penelitian ini bertujuan untuk mengetahui total protein dan profil protein tempe mendoan dengan durasi fermentasi 24, 48, dan 72 jam, guna mengetahui waktu fermentasi terbaik dalam menghasilkan kandungan protein yang tinggi. Analisis profil protein dan total protein dilakukan secara deskriptif yang ditabulasi dalam bentuk tabel dan dihitung rata-rata menggunakan JASP serta Microsoft Excel untuk mengetahui nilai absorbansi kurva baku. Hasil penelitian menunjukkan bahwa total protein tertinggi pada fermentasi 48 jam sebesar 2,65 μg/μL, diikuti oleh fermentasi 72 jam (2,32 μg/μL) dan terendah pada fermentasi 24 jam (1,48 μg/μL). Hasil SDS-PAGE menunjukkan bahwa semua sampel memiliki pita protein minor dengan berat molekul 74 kDa, 70 kDa, 48 kDa, 40 kDa, 31 kDa, 26 kDa, dan 10 kDa. Pada fermentasi 48 jam terdapat pita protein mayor 40 kDa, mengindikasikan bahwa pita yang lebih tebal (Mayor) memiliki konsentrasi protein yang lebih tinggi. Hasil penelitian menunjukkan bahwa fermentasi 48 jam merupakan waktu yang optimal.
Co-Authors Agustin, Afrida Chesarani Arelliza, Fatma Sheilla Dewi, Dini Puspo Dini Puspodewi Farabi, Meka Faizal Haryanti, Anik Mubarok, Akhmad Nugrahaeni, Dian Rahma Nurwahidah, Andi Tenri Pancawati, Yulina Pangesti, Ira Priyanto, Anggih Putri, Reza Amelia Ramadhan, Fahri Rusana Suhada, Wahyu Aji Utami, Tri Fitri Yana Wijayanti, Faulia Triana yunadi, frisca dewi