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Formulation of Edible Film Preparation from Musk Orange Essential Oil (Citrus Microcarpa Bunge) in Inhibiting the Growth of Streptococcus mutans Bacteria and Candida Albicans sp Fungus Anggraini, Chorry Dwi; Mayasari, Ulfayani
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.17369

Abstract

This study aims to describe the formulation of edible film preparations from musk orange essential oil (Citrus Microcarpa Bunge) in inhibiting the growth of Streptococcus mutans and Candida Albicans sp. Fungus. The data collection technique uses laboratory-based experimental testing. Musk lime plants were taxonomically identified at the Herbarium Medanase (MEDA) laboratory, USU Campus, Medan, then processed using the steam distillation method to produce essential oils. The essential oil activity test uses concentrations (1%, 2%, 3%, 4% and 5%) to test its antibacterial and antifungal activity. Musk orange essential oil made in the form of an edible film was then tested for its physical properties as well as antibacterial and antifungal activity against the fungi S. mutans and C. albicans. The yield of essential oils was 0.93539gr/ml from 15kg of 8.5ml sample. The test results of the edible film preparation of the essential oil of musk orange have a distinctive aroma of fresh menthol and musk orange, orange color, smooth texture, sweet taste. Testing the ability of edible film preparations from essential oils has the highest inhibition zone results at a concentration of 4%, 5% which has natural antimicrobial substances. The yield of essential oils was 0.93539gr/ml from 15kg of 8.5ml sample. Essential oil activity tests produced the highest inhibition zones in bacteria and fungi at concentrations of 4% and 5%. The test results of the edible film preparation of the essential oil of musk orange have a distinctive aroma of fresh menthol and musk orange, orange color, smooth texture, sweet taste. Testing the ability of edible film preparations from essential oils has the highest inhibition zone results at a concentration of 4%, 5% which has natural antimicrobial substances.
Isolation and Identification of Biosurfactantproducing Bacteria from the Aerobic Pond of Oil Palm Liquid Waste at PT. Aek Loba Plantation Andini, Diah Ayu; Rasyidah, Rasyidah; Mayasari, Ulfayani
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7811

Abstract

Biosurfactants are natural surfactants derived from bacteria which can degrade oil content in soil or water. Most of these biosurfactant-producing bacteria are often  found in areas polluted by oil and grease, such as aerobic ponds of palm oil liquid waste. The purpose of this research was to isolate and identify biosurfactant-producing bacteria from aerobic ponds of palm oil wastewater at PT. Aek Loba Plantation. This research was conducted using descriptive and identification methods through emulsification tests, morphological characterization, gram staining, biochemical tests, and PCR (Polymerase Chain Reaction) methods for bacteria that had the highest emulsification index values. Based on the results of the emulsification test, it was found that 8 biosurfactant-producing bacterial isolates consisted of 6 bacterial genera and 1 bacterial species that had different emulsification indexes, namely 1 Citrobacter genus isolate (30%), 1 Enterobacter genus bacterial isolate (42.2%) , 2 isolates from the genus Eschericia (35.9% and 36.5%), 1 isolate from the genus Pseudomonas (32%), 1 isolate from the genus Micrococcus (37.5%), 1 isolate from the genus Bacillus, and 1 species of bacteria Klebsiella variicola (42.8%) which is a biosurfactant producing bacteria that has the highest emulsification index value.