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Amelioration of Seminal Plasma Testosterone Concentration in Gembrong Goats after In Vivo Administration of PGF2α Hafizuddin, Hafizuddin; Husnurrizal, Husnurrizal; Siregar, Tongku Nizwan; Eriani, Kartini; Wahyuni, Sri; Ahsan, Muhammad Maulana; Sutriana, Amalia; Anwar, Anwar; Aliza, Dwinna
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.256-261

Abstract

The semen quality of Gembrong goats is lower than other goats and may be related to the low concentration of testosterone hormone. Implementation of reproductive technology using prostaglandin F2α (PGF2α) hormone is beneficial to increase the testosterone hormone in Gembrong goats. This study aimed to determine the effect of PGF2α injection on increasing testosterone levels in Gembrong goats. Male Gembrong goats (n=4), aged 2.5-4 years with similar body condition scores (BCS=3) were used in this study. Goats were divided into two treatment groups (n=2). Goats in group 1 (G1) were injected intramuscularly with 1 ml PGF2α (75 μg), while those in group 2 (G2) were injected with 1 ml physiological NaCl. Semen collection was carried out 30 minutes after treatment using an artificial vagina. Testosterone levels were measured using the enzyme-linked immunosorbent assay (ELISA). The collected data was tabulated and analyzed descriptively. The results showed that the average testosterone concentration of G1 was higher than G2 with respective concentrations of 6.41 ± 0.70 and 2.81 ± 1.75 ng/ml. It was concluded that administration of PGF2α in vivo could increase testosterone concentration in Gembrong goats.
ASSESSING ELECTRICAL RESISTANCE OF VAGINAL MUCUS (ERVM) SCORES IN ESTRUS PHASE OF ACEH COWS AND THEIR ASSOCIATION WITH ESTROGEN AND PROGESTERONE LEVELS Thasmi, Cut Nila; Husnurrizal, Husnurrizal; Melia, Juli; Agung, Nabilah Putroe; Riady, Ginta; Yusuf, M.; Rosmaidar, Rosmaidar; Sayuti, Arman; Hasan, M.; Nazaruddin, Nazaruddin; Hafizuddin, Hafizuddin; Nurliana, Nurliana
Jurnal Kedokteran Hewan Vol 19, No 2 (2025): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i2.44940

Abstract

This study aimed to determine the correlation between ERVM scores and the levels of estrogen and progesterone in Aceh cows during estrus.This study utilized 8 clinically healthy Aceh cows aged 3-5 years. ERVM scores were determined using a DraminskiTMestrus detector probe insertedinto the vagina on the first day of estrus. Estrogen and progesterone concentrations were measured in blood samples collected on the first day of estrususing the enzyme-linked immunosorbent assay (ELISA) method. Data on the correlation between ERVM scores and the levels of estrogen andprogesterone concentrations were analyzed using the Pearson correlation test. The results showed that the average ERVM score during the estrusphase was 192.9216.94 , and the average concentrations of estrogen and progesterone were 103.1344.86 pg/mL and 0.960.60 ng/mL,respectively. Correlation analysis revealed that coefficient correlation (r) values between EVRM and estrogen was -0.607 and with progesterone was-0.149. In conclusion, the ERVM score in Aceh cattle indicates moderate estrus, and the ERVM score obtained in this study is negatively correlatedwith estrogen and progesterone concentrations.
PGF2 supplementation as a strategy to improve post-thawing motility of spermatozoa in Waringin sheep Husnurrizal, Husnurrizal; Azim, Muhammad; Thasmi, Cut Nila; Hafizuddin, Hafizuddin
The International Journal of Tropical Veterinary and Biomedical Research Vol 10, No 1 (2025): Vol 10 (1) May 2025
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v10i1.45784

Abstract

Background and Aim: This study aimed to evaluate the effect of prostaglandin F2 (PGF2) supplementation in semen extenders on the post-thaw sperm motility of Waringin rams.Materials and Methods: Semen was collected from Waringin rams, diluted with Andromed extender, and divided into three treatment groups: control (K0) without PGF2, P1 with 37.5 g PGF2, and P2 with 75 g PGF2.Results : The results showed that post-thaw sperm motility in P1 (49.33%) and P2 (54.00%) was significantly higher than in the control group K0 (25.33%) (P 0.05), although no significant difference was observed between P1 and P2 (P 0.05). Additionally, pre-freezing sperm motility also increased in P1 (75.00%) and P2 (82.33%) compared to K0 (43.67%).Conclusion: It is concluded that PGF2 supplementation in semen extenders can improve the post-thaw motility of Waringin ram spermatozoa, with a 75 g dosage yielding the most optimal effect.