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Juli Melia
Laboratorium Reproduksi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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SYNCHRONIZATION OF GnRH AND PGF2 ON THE REPRODUCTIVE PERFORMANCE IN ACEH CATTLE Melia, Juli; Desita, Ella; Khalifah, Hadid; Justika, Wulan; Armansyah, Teuku; Hasan, Muhammad; Rosmaidar, Rosmaidar; Sayuti, Arman; Aliza, Dwinna; Wahyuni, Sri
Jurnal Kedokteran Hewan Vol 18, No 4 (2024): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i4.41860

Abstract

This study aims to determine the effect of administering gonadotropin-releasing hormone (GnRH) accompanied by prostaglandin F2 alpha (PGF2) on the reproductive performance of female Aceh cattle. In this study, eight female Aceh cattle were used, divided into two groups, group I (K1, n= 3) which received estrus synchronization treatment with PGF2 and group II (K2, n= 5) which received estrus synchronization treatment with PGF2 (25 mg/cow) and gonadotropin-releasing hormone (GnRH, 100 g/cow). GnRH injection in K2 was performed 48 hours after PGF2 injection. The estrus displays measured included onset, duration, and intensity of estrus. Blood collection for estrogen examination was taken from day 1 to day 5 after PGF2 injection, while progesterone examination was taken on days 7, 14, and 21 after artificial insemination. Ovarian response and pregnancy examinations were performed using transrectal ultrasonography (USG). Data on estrus appearance and steroid concentration were analyzed using the Mann-Whitney U test, and differences in pregnancy percentage were analyzed using the chi-square test. In contrast, the number of follicles, dominant follicle diameter, and corpus luteum were analyzed using a split-plot test. Oestrus intensity, oestrus onset, oestrus duration, pregnancy percentage, estrogen and progesterone concentrations, ovarian response in class II follicles, class III follicles, and CL size in K1 vs K2 showed no significant differences (P0.05). The effect of time on ovarian response in class III follicles and CL size showed a significant difference (P0.05). In contrast, the interaction between hormone administration and day of treatment on ovarian response in class II follicles, class III follicles, and CL size showed no significant difference (P0.05). Based on the research results, it was concluded that administration of GnRH accompanied by PGF2 tends to increase the reproductive performance of Aceh cattle.
ASSESSING ELECTRICAL RESISTANCE OF VAGINAL MUCUS (ERVM) SCORES IN ESTRUS PHASE OF ACEH COWS AND THEIR ASSOCIATION WITH ESTROGEN AND PROGESTERONE LEVELS Thasmi, Cut Nila; Husnurrizal, Husnurrizal; Melia, Juli; Agung, Nabilah Putroe; Riady, Ginta; Yusuf, M.; Rosmaidar, Rosmaidar; Sayuti, Arman; Hasan, M.; Nazaruddin, Nazaruddin; Hafizuddin, Hafizuddin; Nurliana, Nurliana
Jurnal Kedokteran Hewan Vol 19, No 2 (2025): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i2.44940

Abstract

This study aimed to determine the correlation between ERVM scores and the levels of estrogen and progesterone in Aceh cows during estrus.This study utilized 8 clinically healthy Aceh cows aged 3-5 years. ERVM scores were determined using a DraminskiTMestrus detector probe insertedinto the vagina on the first day of estrus. Estrogen and progesterone concentrations were measured in blood samples collected on the first day of estrususing the enzyme-linked immunosorbent assay (ELISA) method. Data on the correlation between ERVM scores and the levels of estrogen andprogesterone concentrations were analyzed using the Pearson correlation test. The results showed that the average ERVM score during the estrusphase was 192.9216.94 , and the average concentrations of estrogen and progesterone were 103.1344.86 pg/mL and 0.960.60 ng/mL,respectively. Correlation analysis revealed that coefficient correlation (r) values between EVRM and estrogen was -0.607 and with progesterone was-0.149. In conclusion, the ERVM score in Aceh cattle indicates moderate estrus, and the ERVM score obtained in this study is negatively correlatedwith estrogen and progesterone concentrations.
HISTOPATHOLOGICAL OVERVIEW OF NEW ZEALAND WHITE PSEUDO-PREGNANT RABBIT UTERUS AFTER OVARY TRANSPLANTATION WITH VARYING DURATION OF OVARIES INSIDE THE UTERUS Nazaruddin, Nazaruddin; Fadillah, Nurul Aini; Melia, Juli; Thasmi, Cut Nila; Roslizawaty, Roslizawaty; Sayuti, Arman; Daud, Razali; Adam, Mulyadi; Syafruddin, Syafruddin
Jurnal Kedokteran Hewan Vol 17, No 4 (2023): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i4.34694

Abstract

This study aims to understand the histopathological changes in the pseudo-pregnant rabbit uterus concerning the duration of ovaries inside the uterus during ovarian transplantation. The study used nine New Zealand White (NZW) pseudo-pregnant female rabbits aged 3-5 years, weighed 1.5-2.9 kg. After a 30-day adaptation period, all rabbits were divided into three treatment groups: K1 (n=3) underwent ovarian transplantation for 3 days, K2 (n=3) for 5 days, and K3 (n=3) for 7 days. Pseudo-pregnant was induced using PMSG and hCG. The rabbits were intramuscularly injected with 100 IU of PMSG, followed by intravenous injection of 75 IU of hCG three days later. Ovary transplantation was performed on the 8th day (day 0 being the day of hCG injection). Uterus was collected after the transplantation procedure according to the respective treatment groups. The results showed that in K1, K2 and K3, there was hyperemia in the endometrial epithelium and infiltration of inflammatory cells. Hyperemia and necrosis were more severe in K1 compared to K2 and K3. In K3, the histopathological appearance of the endometrium tended to be normal, with less damage. In conclusion, the histopathological findings of hyperemia and necrosis in the uterus undergoing ovarian transplantation lasted for 3 days compared to 5 and 7 days.
THE EFFECT OF SUPEROVULATION INDUCTION USING FSH ON INCREASING FSH AND LH CONCENTRATIONS IN NEW ZEALAND WHITE RABBITS Wahyuni, Sri; Fathurrahman, Fadhil; Adam, Mulyadi; Syafruddin, Syafruddin; Roslizawaty, Roslizawaty; Hasan, Muhammad; Melia, Juli; Dasrul, Dasrul; Isa, Muhammad; Rosmaidar, Rosmaidar
Jurnal Kedokteran Hewan Vol 18, No 1 (2024): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i1.37513

Abstract

This study aimed to determine the concentrations of Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) in New Zealand White (NZW) rabbits after superovulation induction using FSH. Six female NZW rabbits and one male NZW rabbit were used in this study. Criteria for female NZW rabbits used include having previously given birth, not being pregnant, and having a body weight of 2-3 kg. The rabbits were divided into two treatment groups; the first group was injected with physiological NaCl (NZW1, n= 3), while the second group was injected with FSH (NZW2, n= 3). Injections of physiological NaCl or FSH were administered five times at 12-hour intervals and first injection was given at 20:00 p.m. In NZW1, each injection was given physiological NaCl with volumes of 0.2:0.2+0.2;0.4+0.4 mL, respectively whereas in NZW2, each injection was given FSH with doses of 4:4+4:8+8 mg, respectively. Twelve hours after the last FSH injection, NZW2 rabbits were injected with 100 IU hCG and mated with male rabbit, while NZW1 rabbits were mated without hCG administration. On days 1, 3, and 5 after mating, blood samples were collected for FSH and LH hormone examination using the Enzyme-Linked Immunosorbent Assay (ELISA) method. The FSH concentrations on days 1, 3, and 5 between NZW1 vs NZW2 were 34.781.46 vs 39.753.18; 41.434.90 vs 41.425.14; and 44.471.51 vs 30.130.19 mIU/mL, respectively (P0.05). The LH concentrations on days 1, 3, and 5 between NZW1 vs NZW2 were 33.784.01 vs 40.1812.36; 29.342.24 vs 31.3110.13; and 42.0911.38 vs 30.578.87 mIU/mL, respectively (P0.05). It is concluded that FSH administration does not affect the concentrations of FSH and LH in NZW rabbits. The FSH concentration five days after mating tends to fluctuate, while the LH concentration tends to decrease as the days increased.
DETERMINATION OF OVULATION TIME IN GAYO MARES BASED ON IMAGE OF PREOVULATORY FOLLICLE GROWTH Melia, Juli; Amrozi, A; Supriatna, Iman; Agil, Muhammad
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.26766

Abstract

This study aimed to obtain the ultrasound image of preovulatory follicle growth in gayo mares to predict the ovulation time. This study used three Gayo mares and one gayo stallion for teaser aged 5-10 years and weighed 200-250 kg. Preovulatory follicle growth was observed using transrectal ultrasonography which was performed when the dominant follicle size was 3.5 cm. Observations were made for 3 consecutive estrous periods on gayo mares at relatively the same time. The absence of previous follicular appearance and the presence of hypoechoic area in the same ovary indicate that ovulation has occurred. The result revealed that the diameter of preovulatory follicle of gayo mares was 4.020.32 to 5.050.04 cm. There was no significant follicle growth in the last 48 hours before ovulation and the preovulatory follicle shape altered from spherical to pearshape. Many small follicles (2 cm in diameter) were observed in peripheral area of the ovary indicated the recruitment of follicles which form the first follicular wave. To conclude, the alteration in the size and pearshape of preovulatory follicle and the recruitment of small follicles can be used as a marker to determine the time of ovulation on gayo mares.
PREVALENCE OF MARES GRANULOSA THECA CELL TUMOR (GTCT) IN INDONESIA Amrozi, Amrozi; Melia, Juli; Parwati, Luci; Tumbelaka, Ligaya I.T.A.
Jurnal Kedokteran Hewan Vol 13, No 4 (2019): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i4.12797

Abstract

This study was conducted to determine the prevalence rate of mares GTCT in Indonesia. Ultrasound examination of ovaries were performed on mares in Java and Madura Island of Indonesia. Thirteen mares had been affected by GTCT from 2913 number of examinations. The prevalence rate of GTCT during year 2006 and 2007 were 4.71% and 5.56%, respectively followed by a decrease in prevalence rate of GTCT during 2008-2014. The reproductive cycle in a mare with GTCT had returned to normal 6 months after ovariectomy.
THE EFFECT OF MALACCA LEAVES (Phyllantus emblica) ETHANOLIC EXTRACT ON Plasmodium falciparum GROWTH IN VITRO Asmilia, Nuzul; TR, T Armansyah; Aliza, Dwinna; Melia, Juli; Rahmi, Erdiansyah; Daulay, Lingga Surya Maret
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i4.10215

Abstract

The aim of this research was to find out in vitro antiplasmodium activity of Malacca leaves (Phyllantus emblica) ethanolic extract against Plasmodium falciparum growth. In this study, Plasmodium culture contained 5% parasitemia in ring stage was cultured using candle jar method and antiplasmodial activity test was carried out using microculture. The treatments were divided into 7 groups with four repetitions. K1 as negative control group was given Roswell Park Memorial Institute (RPMI), while K2 as positive control group was given artesdiaquine. Groups K3, K4, K5, K6, and K7 group was added with 100 g/mL, 75 g/mL, 50 g/mL, 25 g/mL, and 5 g/mL of Malacca leaves ethanolic extract, respectively. Antiplasmodial activity was determined by inhibition concentration of 50% parasite growth (IC50). The data were analyzed using ANOVA and followed by Duncan test. The average of parasitemia level in group K1, K2, K3, K4, K5, K6, and K7 were 55.2515.62, 8.502.52, 8.503.00, 9.250.95, 9.002.70, 9.792.06, and 10.752.22, respectively. The average of inhibition percentage in group K1, K2; K3; K4; K5; K6; and K7 were 0.000.00%, 84.624.55%; 84.625.43%; 83.261.73%; 83.714,90%; 82.353,73%; and 80.546.83%, respectively (P0.01). The results showed that the administration of malacca leaves ethanolic extract significantly affect (P0.01) the inhibition of Plasmodium growth as compared to group K1 (negative control). Probit analysis reveals the IC50 value was 3.889 g/mL. In conclusion, all doses of malacca leaves ethanolic extract used in this study was able to inhibit Plasmodium falciparum growth with IC50 value was 3.889 g/mL.
THE IMAGE OF EMBRYO AND FETAL OF ETAWA CROSSBRED GOATS USING ULTRASONOGRAPHY AT DIFFERENT GESTATIONAL AGES Melia, Juli; Fauziah, Fauziah; Hamdan, Hamdan; Panjaitan, Budianto; Syafruddin, Syafruddin; Armansyah, Teuku; Siregar, Tongku Nizwan
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i2.3768

Abstract

This study aimed to estimate the gestational age of Etawa crossbred goats based on ultrasound images of embryo and fetal usingtranscutaneous ultrasonography. Etawa crossbred does (n= 5) were synchronized using double PGF2 injection with 10 days interval and matednaturally. Early pregnancy was diagnosed on 20th day after mating base on isoechogenic image of the embryo (2.650.75 mm) andhypoechogenic appearance of gestational sac. On 30th day of the first trimester, the embryo size was 8.310.42 mm. The embryo reached10.440.51 mm on 35th day. On 60th day, the existence of the fetal were clearly appeared along with isoechogenic of umbilical cord,hyperechogenic os cranium (25.50.1 mm), and body length (51.831.59 mm). On 75th day, the diameter of the os cranium was 33.030.20 mm,body length of 1101.73 mm, and bones structures appeared hyperechogenic for os thoracalis and os vertebrae. The placenta was isoechogenoicwith diameter of 23.30.2 mm. On 90th day, the placenta dominated the uterus (22.671.70 mm) and reached its maximum diameter on 145th day(33.732.91 mm). In conclusion, early pregnancy detection of Etawa crossbred goats using transcutaneous ultrasonography could be performedon 20th day after mating
CORRELATION BETWEEN PROGESTERONE HORMONE CONCENTRATION AND NUMBER OF FETUSES OF ETAWA CROSSBRED GOAT IN DIFFERENT GESTATION PERIOD Syafruddin, Syafruddin; Rizal, Khairul; Rosmaidar, Rosmaidar; Melia, Juli; roslizawaty, roslizawaty; Zuhrawati, Zuhrawati; Rahmi, Erdiansyah; Abrar, Mahdi
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.8687

Abstract

This study aimed to investigate the correlation between progesterone hormone concentration and number of fetus as well as the difference in progesterone hormone concentration from different gestation periods in Etawah crossbred (EC). Five EC goats used in this study were injected with 2 mL of 5.5 mg prostaglandin F2 (PGF2, Capriglandin) intramuscularly, with an interval of 10 days. Goats that showed symptoms of estrus were mated naturally with male goats. Blood samples were taken on the 7th, 14th, 21st, and 75th days of gestation. Progesterone hormone concentration was determined using enzyme linked immunosorbent assay (ELISA). The number of fetus was determined at 35th gestational day using ultrasonography (USG). Four out of 5 goats were found to have 1 fetus and 1 goat had 2 fetuses. The progesterone concentration on the 7th, 14th, 21st, and 75th days of gestation in goats with single fetus were 5.21, 7.78, 11.97, and 18.78 ng/mL, respectively, while in goat with two fetuses were 8.44, 14.53, 16.81, and 22.73 ng/mL, respectively. The correlation (r) between progesterone hormone concentration and number of fetus on the 7th, 14th, 21st, and 75th days of gestation were 0.442, 0.854, 0.592, and 0.757, respectively. It is concluded that progesterone concentrations are highly correlated to the number of fetus in each gestation period in EC goats.
ACEH CATTLE FOLLICLE DYNAMIC UNDER ENVIRONMENTAL HEAT STRESS Armansyah, Teuku; Siregar, Tongku Nizwan; Aliza, Dwinna; Melia, Juli; Meutia, Nellita; Panjaitan, Budianto; Hafizuddin, Hafizuddin; Adam, Mulyadi; Abrar, Mahdi
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.6377

Abstract

The objective of this research is to know Aceh cattle follicle dynamic under environmental heat stress condition. This research was conducted on December 2012 until August 2013. Animals used in this research were 20 Aceh cattle aged 5-8 years old, weighing of 150-250 kg, and had at least 2 regular cycles. All cattle used were clinically in good body condition score. The cattle were divided into two groups of 10, used in two different period of times (December-January and July-August) to examine the effect of environmental heat to their follicle dynamic. Each group is separated into two different keeping management; one group was kept in pens while the others in the pasture, 5 cattle in each management group. Research timing was based on information acquired from Indrapuri Agency for Meteorology, Climatology, and Geophysics (BMKG) which predicted that extreme weather would last from July to August while December to January would be relatively normal in Aceh region. All cattles estrous cycle were synchronized by 5 mg/mL of PGF2 intramuscularly (Lutalyse, Pharmacia Upjohn Company, Pfizer Inc.). Ultrasonography examination was performed to monitor ovary follicles growth and dynamic during one cycle. Days during ovulation marked by standing heat was regarded as Day 0 of estrous cycle. Follicular dynamic examination during estrous cycle on December-January and also on July-August, both penned or pastured cattle showed the follicle waves that was 3 follicle growth waves. The size of follicle growth on first wave (1st DF) on cattle kept in pen on July-August and December-January were relatively similar. The size of follicle growth on first wave for cattle kept in pasture on July-August and December-January were relatively similar. To conclude, Aceh cattles follicle dynamic is not change during environment heat stress condition and is not affected by different cattle-keeping management.
Co-Authors Ade Ocktaviani, Ade Agik Suprayogi Amiruddin Amiruddin Amrozi Amrozi, A Andi Novita Arman Sayuti Bondan Achmadi, Bondan budianto panjaitan Cut Nila Thasmi, Cut Nila Dasrul Dasrul Daulay, Lingga Surya Maret Desita, Ella Dwinna Aliza Erdiansyah Rahmi Fadillah, Nurul Aini Fathurrahman, Fadhil Fauziah Fauziah Ginta Riady Hafizuddin Hafizuddin Hamdan h Hamdan Hamdan Hamdani Budiman Husnur rizal Husnurrizal, Husnurrizal Iman Supriatna Ira Khubairoh Marpaung, Ira Khubairoh Justika, Wulan Khalifah, Hadid M. Hasan M. Yusuf Mahdi Abrar maikhar gita eldora Muhammad Agil Muhammad Hasan Muhammad Isa Mulyadi Adam, Mulyadi Muslim Akmal N. Nazaruddin Nabilah Putroe Agung Nellita Meutia, Nellita NURLIANA NURLIANA Nuzul Asmilia Parwati, Luci Razali Daud Razali R Reni Ayunanda, Reni rina aulia barus Rizal, Khairul Roslizawaty r Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar, Rosmaidar Sri Wahyuni Syafruddin s Syafruddin Syafruddin T. Armansyah Teuku Reza Ferasyi Tongku Nizwan Siregar tongku nizwar siregar TR, T Armansyah Yudha Fahrimal yusmadi y Zuhrawati Zuhrawati, Zuhrawati