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All Journal Teknotan: Jurnal Industri Teknologi Pertanian AGRITEKNO, Jurnal Teknologi Pertanian Jurnal TAMBORA Jurnal Biosense Journal of Community Development Fruitset Sains : Jurnal Pertanian Agroteknologi Asia Pacific Journal of Sustainable Agriculture, Food and Energy (APJSAFE) Tabela Jurnal Pertanian Berkelanjutan Jurnal Javanica: Jurnal Terapan Agribisnis Meta: Journal of Science and Technological Education Agrimas : Jurnal Pengabdian Masyarakat Bidang Pertanian
Nuraisyah, Anni
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Meta: Journal of Science and Technological Education

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Colony Growth and Morphological Characterization of Sugarcane Root Bacteria and Sugarcane Field Exploration Bacteria in Fermented Liquid Irawan, Triono Bambang; Kusuma, Indra Satria; Nuraisyah, Anni; Soelaksini, Liliek Dwi
META: Journal of Science and Technological Education Vol. 3 No. 1 (2024)
Publisher : Aspirasi Masyarakat Intelektual Islam Nusantara

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Abstract

Rhizosphere bacteria play crucial roles in sugarcane fertility by providing nutrients, protecting against pathogens, and producing growth hormones. Exploring these bacteria can enhance sugarcane productivity through biofertilizers, biostimulants, and bioprotectants, reducing the need for costly inorganic fertilizers. This exploration is vital for optimizing sugarcane cultivation. The aims of the study were to determine the growth of bacterial colonies over 1, 2, and 3 weeks and to identify the morphology of sugarcane root bacteria and sugarcane exploratory bacteria growing in fermented liquid. The results showed: (i) Growth in the number of sugarcane root bacterial colonies was 7.7 × 10⁷ CFU/ml in week 1, increasing to 28.8 × 10⁷ CFU/ml in week 2, and further to 58 × 10⁷ CFU/ml in week 3 in fermented liquid. The growth of bacterial colonies exploratory of sugarcane land was 3.4 × 10⁷ CFU/ml in week 1, increasing to 19.7 × 10⁷ CFU/ml in week 2, and further to 62 × 10⁷ CFU/ml in week 3 in fermented liquid; (ii) Morphology of sugarcane root bacterial colonies from week 1 to week 3 included colony colors: yellow, pink, blue, milky white, blue center; colony sizes: pinpoint, medium, large; colony shapes: circular, irregular; elevation: convex, flat; surface textures: smooth, mucoid; edges: regular, irregular. Bacterial cell shape was bacilli and Gram-positive; and (iii) The morphology of bacterial colonies from sugarcane field exploration from week 1 to week 3 included colony colors: yellow, pink, blue, milky white, blue center; colony sizes: small, medium, large, pinpoint; colony shapes: circular, irregular; elevation: convex, flat, umbonate, raised; surface textures: smooth, mucoid; edges: regular, irregular.
Colony Growth Rate and Morphological Characterization of Bovine Rumen Bacteria in Fermented Liquid Irawan, Triono Bambang; Kusuma, Indra Satria; Soelaksini, Liliek Dwi; Nuraisyah, Anni
META: Journal of Science and Technological Education Vol. 3 No. 2 (2024)
Publisher : Aspirasi Masyarakat Intelektual Islam Nusantara

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Bovine rumen bacteria consist of diverse microorganisms essential for processing manure, compost, and liquid organic fertilizer, enhancing soil fertility and nutrient availability while suppressing pests and pathogens. These bacteria include cellulose-digesting species such as Bacteroides succinogenes, Ruminococcus flavefaciens, and Ruminococcus albus, which produce cellulase enzymes for organic matter decomposition. Morphologically, cellulolytic bacteria are categorized into coccus forms (Ruminococcus flavefaciens and Ruminococcus albus) and rod forms (Bacteroides succinogenes and Butyrivibrio fibrisolvens), with Gram-positive and Gram-negative cell walls. This study examined the growth and morphology of bovine rumen bacteria in fermented liquid over three weeks. Colony counts increased from 4.75 × 10⁶ CFU/mL in week 1 to 2.92 × 10⁷ CFU/mL in week 2 and 6.2 × 10⁸ CFU/mL in week 3. Colony morphologies included colors (pink, yellow, blue center), sizes (small, medium, large), shapes (circular, irregular), elevations (raised, convex, unbonated), surfaces (smooth, mucoid), and edges (regular, irregular). Predominant bacterial cell shapes were bacillus, with Gram-positive staining. These findings underscore the potential of bovine rumen bacteria in organic waste fermentation and nutrient cycling.
Co-Authors Abdul Madjid Abdurrahman Salim Afgani, Chairul Anam Ais, Mamluatul Anggraeni, Dwi Ayu Annisa Lutfi Alwi Bambang Irawan, Triono Diana, Luluk E Dwi Soelaksini, Liliek Eko Priyantono Fadilah, Fitriyatul Fatimah, Titien Harsantyo, Himawan Daffa Ika Lia Novenda Irawan, Triono Bambang Isworo, Rhestu Isworo, Rhestu Isworo Juliansyah, Alfian Komarudin, Nurul Amri Kusuma, Indra Satria Kusuma, Satria Indra Liliek Dwi Soelaksini M. Amien Rais Mikhratunnisa, Mikhratunnisa Nisa Budi Arifiana Nurfiana, Yuni Pramudianto, Pascal R Prayogo, M. Mikail R P Rodiahwati, Wawat Rudi Wardana Satria Indra Kusuma Setyo Andi Nugroho Silsilatilma’wa, Silsilatilma’wa Soelaksini, Liliek Dwi Sofyan Hadi Triono Bambang Irawan Wardati, Irma Zulisma, Elok D