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All Journal Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) JTSL (Jurnal Tanah dan Sumberdaya Lahan) Jurnal Sains & Teknologi Lingkungan Jurnal Biologi Tropis Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Makara Journal of Science Prosiding Seminar Nasional Biologi BIODIVERS - BIOTROP Scientific Magazine Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
ROSITA, Risa
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Social Sciences

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Effectivity of Signal Grass (Brachiaria decumbens) Enrich with Microorganism to Absorb Heavy Metals (Pb) Rosita, Risa
BIODIVERS - BIOTROP Science Magazine Vol. 1 No. 2 (2022): BIODIVERS (BIOTROP Science Magazine) - Save Biodiversity for Sustainable Well-b
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56060/bdv.2022.1.2.1978

Abstract

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Hand Soap Based on Moringa Leaf to Reinforce Agro-Eco-Edu-Tourism in Generating Creative Younger Generation Sugiyanto, Wardani; Yulvianah; Rosita, Risa; Darwati, Aan
BIODIVERS - BIOTROP Science Magazine Vol. 2 No. 1 (2023): BIODIVERS (BIOTROP Science Magazine) : Agro-Eco-Edu-Tourism in Managing Tropica
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56060/bdv.2023.2.1.1995

Abstract

Teaching Factory is a learning model that can be adopted in the Agro-Eco-Edu-Tourism (AEET) program. The learning model products in Teaching Factory are soap and hand sanitizer. The study's results reported that hand washing soap with the addition of Moringa leaf extract could inhibit the growth of Staphylococcus aureus and Escherichia coli bacteria that cause diarrheal disease. Diarrheal disease is still a public health problem in developing countries such as Indonesia because of its high morbidity and mortality. Diarrhea occurs due to the spreading S. aureus and E. coli bacteria, most often transmitted from hand to hand. Washing hands using soap and hand sanitizer is an activity that aims to remove dirt on hands.
Keanekaragaman Fungi Mikoriza Arbuskula (FMA) dari Rizosfer Tanaman Hias: Karakterisasi Morfologi dan Seleksi Hazra, Fahrizal; Rosita, Risa; Fadhilla Br Siregar, Rizky Tri
COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat Vol. 4 No. 11 (2025): COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/comserva.v4i11.3008

Abstract

Salah satu tantangan dalam budidaya tanaman hias adalah optimalisasi pertumbuhan tanpa ketergantungan berlebihan pada pupuk dan pestisida kimia. Oleh karena itu, pemanfaatan FMA sebagai biofertilizer alami dapat menjadi solusi yang berkelanjutan untuk meningkatkan kualitas dan ketahanan tanaman hias. Eksplorasi keanekaragaman FMA dari rhizosfer tanaman hias menjadi penting untuk mengidentifikasi spesies-spesies yang berpotensi dalam meningkatkan pertumbuhan tanaman secara alami. Karakterisasi morfologi spora FMA dapat memberikan informasi mengenai spesies yang ada dan potensinya dalam mendukung ekosistem tanah. Penelitian ini bertujuan untuk mengeksplorasi dan mengidentifikasi morfologi FMA yang diisolasi dari rhizosfer berbagai jenis tanaman hias, guna memberikan wawasan lebih lanjut mengenai keanekaragaman dan peran ekologi fungi ini. Tahapan penelitian meliputi koleksi tanaman hias, sampling tanah, isolasi Fungi Mikoriza Arbuskula (FMA), pengelompokkan dan identifikasi morfologi spora Fungi Mikoriza Arbuskula (FMA), dan seleksi FMA pada kultur spora. Hasil penelitian menyatakan bahwa Kepadatan spora tanaman Soka (Ixora coccineal L.) memiliki kepadatan spora yang paling tinggi senilai 36 spora. Hal ini diketahui bahwa tanaman soka memiliki kondisi akar yang menguntungkan untuk kolonisasi mikoriza. Hasil identifikasi total spora Glomus sp. lebih banyak di temukan karena memiliki tingkat adaptasi yang lebih tinggi terhadap lingkungan dibandingkan genus lainnya. diperoleh data bahwa spora FMA pada kode D1, D2, D5, D6, D7, dan D8 berhasil mengkolonisasi perakaran tanaman inang sorghum yang ditandai dengan terbentuknya hifa. Kolonisasi FMA dimulai ketika spora berkecambah dan menghasilkan hifa eksternal yang tumbuh menuju akar tanaman inang. Glomus sp. merupakan jenis FMA yang memiliki kolonisasi FMA yang tinggi karena memiliki kemampuan kolonisasi yang cepat dibandingkan jenis FMA lain. Pada kultur spora kode D3 dan D4, tidak terjadi kolonisasi dan tanaman menjadi mati. Hal ini disebabkan oleh spora yang kurang adaptif. Akibatnya, tanaman sorgum tidak mendapat manfaat dari simbiosis mikoriza.
REHABILITASI TANAH PASCA TAMBANG BATU BARA MENGGUNAKAN RUMPUT BEDE (Brachiaria decumbens) MELALUI PEMANFAATAN MIKORIZA DAN Azospirillum Rosita, Risa; Eris, Deden Dewantara; Faulina, Sarah Asih; Suryani, Dewi
Prosiding Seminar Nasional Biologi Vol. 12 (2024)
Publisher : Prosiding Seminar Nasional Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Rehabilitasi lahan pasca tambang batu bara dapat dilakukan dengan memadukan pembenahan lahan, pemilihan jenis tanaman yang tepat dengan kriteria cepat tumbuh dan tahan terpapar matahari, cepat terdekomposisi, serta memiliki sistem perakaran baik dan dapat bersimbiosis dengan mikroorganisme tertentu. Penelitian ini bertujun untuk mempelajari pengaruh pemberian FMA dan Azospirillum secara tunggal maupun gabungan dalam meningkatkan pertumbuhan rumput Bede (Brachiaria decumbens) pada tanah pasca tambang batu bara. Mikoriza dan Azospirillum yang digunakan diharapkan dapat meningkatkan pertumbuhan rumput Bede yang ditanam pada tanah revegetasi dan overburden pasca tambang batubara. Perlakuan T1P11 (gabungan NPK 50% + dolomit 50% + Mikoriza 10 g + Azospirillum 5ml) pada tanah revegetasi T1P11=10.23 g) dan overburden (T2P11=4.41 g) mampu menaikan berat basah rumput dibandingkan kontrol dan perlakuan lainnya. Pada tanah revegetasi gabungan perlakuan tersebut (T1P11) menaikan pH masam tanah dari 4.8 menjadi mendekati normal 5.5 serta menaikan nilai KTK tanah dari 14 menjadi 19 dalam waktu 1 bulan setelah aplikasi. Sementara pada overburden (T2P11) pH masam tanah naik dari 4.3 menjadi 4.5 dan nilai KTK dari 12 menjadi 16 setelah 1 bulan aplikasi. Uji molekuler dari spora tunggal Glomus etunicatum membuktikan bahwa inokulum mikoriza yang digunakan dalam penelitian terjaga kemurniannya sedangkan Azospirillum yang digunakan diisolasi dari perakaran rumput Bede. Penggunaan mikoriza dan Azospirillum dapat mengurangi 50% penggunaan pupuk NPK dan dolomit dalam meningkatkan pertumbuhan rumput B.decumbens. Rehabilitasi lahan pasca tambang perlu dilakukan untuk memulihkan kembali lahan yang sudah terdegradasi.
THE DIVERSITY AND CHARACTERISATION OF CELLULOLYTIC BACTERIA ISOLATED FROM VARIOUS CONDITIONS OF EMPTY FRUIT BUNCHES IN OIL PALM PLANTATION Agam Pamungkas, Edo; Madusari, Sylvia; Putri, Halida Adistya; Rosita, Risa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10238

Abstract

The utilisation of oil palm empty fruit bunches (EFB) remains underexplored. Harnessing cellulolytic microorganisms for the production of cellulase enzymes offers sustainable approach to addressing waste management challenges while aligning with the principles of the Sustainable Development Goals (SDGs) and address waste management challenges. This study aims to isolate, characterize, identify, and test the potential cellulase activity of cellulolytic bacteria from EFB taken from three different locations: PO code from organic fertilizer plantations (POU1, POU2, POU3), PL code from oil palm plantations (PLU1, PLU2, PLU3), and PK code from Sulung mills (PKU1, PKU2, PKU3). This study used three isolated cultures in its testing. The research process includes sample preparation, bacterial isolation, gram staining, catalase test, hypersensitivity test, DNA amplification, bioinformatics analysis and cellulase activity analysis. The results of the bacterial isolation obtained 28 colonies. The results of the characterisation were all 3 non-pathogenic bacterial isolates, with a positive catalase test. The result of staining Gram-negative with bacilli-shaped bacteria. The amplification results obtained a band size of 1500 bp. The results of the identification obtained the species Aeromonas enteropelogenes, Nitrosomonas stercoris, and Methylobacillus caricis. The results of phylogenetic analysis showed low homology. Cellulase activity of six positive isolates with medium ability isolates code POU3 (1.3), PLU2 (1.0), PLU3 (1.0); low isolates POU1 (0.2), POU2 (0.2), PLU (0.8) and 3 negative isolates no enzyme activity PKU1 (-1), PKU2 (-1), and PKU3 (-1).
Direct Pellet PCR: Rapid Extraction-Free Method for Identification of Cellulolytic Bacteria from Empty Fruit Bunch Pamungkas, Edo Agam; Madusari, Sylvia; Putri, Halida Adistya; Rosita, Risa
Jurnal Biologi Tropis Vol. 26 No. 1 (2026): Januari-Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i1.11225

Abstract

Oil Palm (Elaeis guineensis Jacq.) is a major industrial crop producing large amounts of empty fruit bunches (EFB), which necessitate rapid molecular methods to support microbial biodiversity studies and biotechnology applications. This study evaluated the effectiveness of Direct Pellet PCR as a rapid, extraction-free method for the molecular identification of cellulolytic bacteria isolated from EFB. Bacterial cell pellets were directly used as PCR templates to amplify the 16S rRNA gene without prior DNA extraction. The method consistently produced clear and specific amplicons of approximately 1500 bp for all isolates, indicating reliable PCR amplification. Sequence analysis showed similarity values ranging from 68.82% to 80.43% compared with reference sequences in the NCBI database, suggesting low similarity to currently available references. These findings demonstrate that Direct Pellet PCR is an efficient and cost-effective approach for rapid DNA amplification and preliminary molecular screening of cellulolytic bacteria from lignocellulosic substrates.
PERAN FUNGI MIKORIZA ARBUSKULA (FMA) DALAM MENINGKATKAN PERTUMBUHAN RUMPUT BEDE (Brachiaria decumbens Stapf.) PADA TANAH TERCEMAR TIMBAL (Pb) Hazra, Fahrizal; Rosita, Risa; Tamimi, Thariq Final
JTSL (Jurnal Tanah dan Sumberdaya Lahan) Vol. 13 No. 1 (2026)
Publisher : Departemen Tanah, Fakultas Bio-industri Pertanian dan Kehutanan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtsl.2026.013.1.16

Abstract

Lead (Pb) contamination in soil has a deleterious effect on agricultural land quality. Remediation using Arbuscular Mycorrhizal Fungi (AMF) and signal grass (Brachiaria decumbens Stapf.) has been identified as an effective strategy. This study aims to (1) evaluate the effectiveness of AMF on sorghum growth, (2) compare the performance of single and combined AMF applications, and (3) assess the effect of AMF on the growth of signal grass. The experiment was conducted at the SEAMEO BIOTROP Laboratory using a completely randomized factorial design with three factors: (1) Pb concentration (0, 50, 100 ppm); (2) AMF application (no AMF, Glomus fasciculatum (GF), and a combination of Glomus fasciculatum + Gigaspora margarita (GF+GM)); and (3) soil conditions (sterile and non-sterile). The results showed that AMF propagation enhanced sorghum growth, with the combination treatment (GF+GM4) performing best, while the single treatment (GF5) produced the highest spore density. Single AMF propagation (GF) was more effective for spore multiplication, whereas the multi-genus combination supported greater plant growth. The GF+GM treatment also produced optimal signal grass growth, with the highest plant height at P2F3T2 (29.33 cm) and the highest number of leaves at P2F1T1 (4.06 leaves). The mechanism of Pb accumulation in roots, its translocation to the canopy, and the role of AMF in restricting Pb ion transport while enhancing nutrient uptake demonstrate that integrating phytoremediation and bioremediation effectively reduces Pb toxicity.
Co-Authors Agam Pamungkas, Edo Apriana, Edwin Aslan Aslan, Aslan Ayu Astari, Risya Bayuaji, David Yudhoyono Cahyono Agus Darwati, Aan Dewantara Eris, Deden Dewi Suryani Dewi Wulandari Dewi, Amelia Cantrika Eris, Deden Dewantara Fadhilla Br Siregar, Rizky Tri Fahrizal Hazra Fikri Maulana, Ahdiar Fitra, Allegra Aura Halimah, Rohima Novianti Irdika Mansur Jannah, Alifia Wardhatul Jekti Prihatin Pamungkas, Edo Agam Purnajaya, Agus Putri, Halida Adistya Rahmadani, Ghina Radhiyya Rohman, Abdu Salma, Fahriyah Ulimatus Sarah Asih Faulina, Sarah Asih Sri Widayanti Sugiyanto, Wardani Suryadi, Jennifer Claudia Sylvia Madusari Tamimi, Thariq Final Wachju Subchan Yulvianah Zulhamsyah Imran