Article Per Year (5 Year)
p-Index From 2021 - 2026
1.235
P-Index
This Author published in this journals
All Journal Journal of Mathematical and Fundamental Sciences Indonesian Journal of Biotechnology Science and Technology Indonesia Buletin Ilmiah Sarjana Teknik Elektro
Rizal Azis
Universitas Indonesia
Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Electrical & Electronics Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 7 Documents
Search

 1 
The Potential of Ethanol Extract of Pasak Bumi Roots (Eurycoma longifolia Jack) as an Anti-Prostate Cancer In Vitro Against PC-3 Cells Kania, Nia; Rahman, Eka Yudha; Priyandoko, Didik; Sabrina, Adilah Hafizha Nur; Widowati, Wahyu; Azis, Rizal; Annaba, Aziz; Hadiprasetyo, Dhanar Septyawan; Alexandro, Garry
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.452-466

Abstract

The prevalence of prostate cancer cases in men is expected to continue increase. In 2040, it is estimated that there will be 2.293.818 new cases and a 1.05% increase in the death rate due to prostate cancer. Eurycoma longifolia Jack roots extract (ELE) has potential as an alternative treatment. This study aims to analyze ELE potential as an anti-prostate cancer agent through in silico assay and in vitro assays on the prostate cancer cell line (PC-3). ELE compounds were docked against Casp-3, Casp-8, HAX-1, p27, and PTEN. In vitro assays on PC-3 cells were used, namely cell viability (WST-8), ROS levels; cell cycle; and cell apoptosis (flow cytometry), PC-3 cell senescence (μ-Galactosidase staining), Casp-3; Casp-8; HAX-1; p27; and PTEN gene expression (qRT-PCR). All proteins target were successfully docked with ELE compounds and presented binding interactions. ELE is known to reduce viability, intracellular ROS levels, live cells, necrosis, and reduce HAX-1 gene expression, and inhibit the cell cycle G0/G1 phase. ELE can also increase inhibition, senescence, late and early apoptosis, and Casp-3, Casp-8, p27, and PTEN gene expression. ELE 100 μg/mL is the most effective concentration. ELE has potential as an anti-prostate cancer agent through apoptosis, cell cycle, and antioxidant pathways
Conditioned Medium of IGF1-Induced Human Wharton’s Jelly Mesenchymal Stem Cells Effects on Sox9 Gene Expression and Level of COL2 and IL1β in Osteoarthritic Chondrocytes Widowati, Wahyu; Kusuma, Hanna Sari Widya; Azis, Rizal; Eltania, The Fransiska; Nindya, Faradhina Salfa
Journal of Mathematical and Fundamental Sciences Vol. 56 No. 3 (2024)
Publisher : Directorate for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2024.56.3.1

Abstract

Wharton’s jelly mesenchymal stem cells (WJMSCs) hold promises for treating osteoarthritis (OA) due to their great capacity for regeneration and their accessibility. However, obstacles like poor cell survival and differentiation prevent them from being used in transplants. Thus, this study examined WJMSCs’ Conditioned Medium (CM) (WJMSCs-CM) for OA treatment. In order to determine how well IL1β-induced chondrocyte cells (IL1β-CHON002) heal OA-damaged cells, they were treated with both IGF1-induced and uninduced WJMSCs-CM. Key parameters measured were IL1β, Sox9 and COL2. The STRING database was explored to evaluate the proteins contained in WJMSCs-CM and to see their interactions and CM potential in OA exposure. The STRING database indicates WJMSCs-CM to possess the ability to manage OA because of the growth factors they contain, which raise the expression of cartilage marker genes. The results indicated that 15% WJMSCs-CM with 150 ng/mL IGF1 had the highest Sox9 expression, significant reduction in pro-inflammatory mediators, and an increase in COL2 levels. Additionally, 14 days of treatment resulted in better outcomes compared to 7 days. CM from WJMSCs treated with IGF1 with a concentration of 15% exhibited the highest level for each parameter. Both durations showed difference across parameters, highlighting the potential for OA therapy.
Potential of Secretome Hydrogel for Wound Healing in LPS- and Scratch-Induced BJ Cells as an Inflammation Model Widowati, Wahyu; Rahmat, Deni; Faried, Ahmad; Nainggolan, Ita Margaretha; Priyandoko, Didik; Wargasetia, Teresa Liliana; Sugiaman, Vinna Kurniawati; Triharsiwi, Dwi Nur; Qlintang, Sandy; Murti, Harry; Azis, Rizal; Jeffrey, Jeffrey
Science and Technology Indonesia Vol. 10 No. 4 (2025): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.4.1242-1254

Abstract

Wound healing often requires specialized interventions to accelerate recovery due to prolonged inflammation and limited regenerative factors. Umbilical Cord Mesenchymal Stem Cells secretome (UCMSCs) comprises various cytokines and growth factors that can promote wound healing. This study aims to analyze the potential of a secretome-based hydrogel as a wound-healing agent using BJ fibroblast cells induced by lipopolysaccharide (LPS) and scratch injury as an inflammation model. The secretome hydrogel was formulated using Carbopol, Hydroxypropyl Methylcellulose (HPMC), Hydroxyethyl Cellulose (HEC), and secretome. Cytotoxicity was conducted using the WST-8 assay, while cell migration was evaluated through a scratch assay. Tumor Necrosis Factor-???? (TNF-????), Nuclear Factor kappa-B (NF-????B), and Interleukin-8 (IL-8) gene expression were analyzed via qRT-PCR. Additionally, malondialdehyde (MDA) levels were measured for oxidative stress assessment, whereas Connective Tissue Growth Factor (CTGF) and Transforming Growth Factor-????1 (TGF-????1) levels were quantified using ELISA and colorimetric assays. The secretome hydrogel exhibited no cytotoxic effects on BJ fibroblast cells and significantly enhanced cell migration. Moreover, it reduced the TNF-????, IL-8, and NF-????B expression, indicating anti-inflammatory activity. The hydrogel also decreased MDA levels while increasing TGF-β1 and CTGF expression, suggesting antioxidant properties and enhanced tissue regeneration in the inflammatory model. The secretome-based hydrogel presents a promising therapeutic approach for promoting chronic wound healing by modulating inflammation, reducing oxidative stress, enhancing tissue regeneration, and stimulating fibroblast migration.
Anti‐inflammatory properties of conditioned medium from human Wharton’s jelly mesenchymal stem cells Azis, Rizal; Widowati, Wahyu; Gunanegara, Rimonta Febby; Kusuma, Hanna Sari Widya; Triharsiwi, Dwi Nur; Hadiprasetyo, Dhanar Septyawan; Gondokesumo, Marisca Evalina; Mars, Nicholas
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.107035

Abstract

Acute respiratory distress syndrome (ARDS) is a critical respiratory dysfunction triggered by intense in‐ flammation, microvascular damage, and increased epithelial and pulmonary vascular permeability. Human Wharton’s jelly mesenchymal stem cells (hWJMSCs) possess regenerative and anti‐inflammatory activities through the cytokines, chemokines, and growth factor secretion. The development of anti‐inflammatory agents derived from hWJMSCs has become one of the therapeutic solutions. Instead of direct cell use of hWJMSCs, their conditioned medium (CM) provides a cell‐free approach that delivers bioactive factors while minimizing the risks associated with stem cell transplantation. This study aims to measure the levels of vascular endothelial growth factor‐α (VEGF‐α), epidermal growth factor‐β (EGF‐β), interleukin‐10 (IL‐10), and hepatocyte growth factor (HGF) in CM‐hWJMSCs under non‐starvation and starvation conditions (24, 48 and 72 hours) using ELISA. The anti‐inflammatory potential of these factors was then analyzed through molecular docking with pro‐inflammatory cytokines. VEGF‐α, EGF‐β, IL‐10 and HGF levels were measured across all conditions. VEGF‐α ranged from 2590.37 to 3613.92 ng/mg protein; EGF‐β 347.01–504.43 ng/mg; IL‐10 302.59–729.28 pg/mg; and HGF 1747.20–2903.52 ng/mg. The molecular docking revealed strong binding between VEGF‐α, EGF‐β, IL‐10 and HGF with pro‐inflammatory cytokines, namely IL‐1β, IL‐6 and TNF‐α. VEGF‐α had the strongest bond with TNF‐α (–1162.3 kJ/mol), while EGF‐β formed the most hydrophobic and hydrogen interactions. The findings suggest that CM‐hWJMSCs, enriched with anti‐inflammatory and regenerative cytokines, may serve as a promising candidate for modulating the inflammatory pathways involved in ARDS pathogenesis. Longer starvation increased the secretion of VEGF‐α, EGF‐β, IL‐10 and HGF. These factors are known to promote angiogenesis, regulate immune responses, and protect against epithelial injury, thereby supporting the anti‐inflammatory and regenerative potential of hWJMSCs‐CM for ARDS therapy.
Grid-Aligned Patchification for Deep Learning-Based Macrophage Detection in Unstained Brightfield Haemocytometer Images Mohammad Ikhsan; Zino Ramdani Suharto; Rizal Azis; Basari Basari
Buletin Ilmiah Sarjana Teknik Elektro Vol. 8 No. 3 (2026): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/biste.v8i3.16005

Abstract

Manual cell counting from haemocytometer images is slow, subjective, and operator-dependent, especially in unstained brightfield microscopy where cell boundaries and viability-related morphology are difficult to distinguish. Although prior cell detection models have mainly been evaluated on stained or fluorescence images, systematic comparisons between fine-tuned detectors and zero-shot cell segmentation models remain limited for unstained brightfield haemocytometer images. This study presents a controlled 2×2 factorial benchmark of patchification and augmentation across five detection approaches, with variance-decomposition analysis and comparison of fine-tuned versus zero-shot deployment modes. Using 24 unstained brightfield RAW 264.7 macrophage images with 6,307 polygon-level annotations, including 28.8% dead cells, we evaluated four preprocessing scenarios under six-fold stratified cross-validation. Faster R-CNN, Mask R-CNN, and YOLOv11n-Seg were fine-tuned within each fold, whereas Cellpose and StarDist were applied zero-shot. Grid-aligned patchification improved bounding-box mAP50 by 2.6–8.4× across all fine-tuned architectures (paired Wilcoxon p = 0.016, Cohen’s d > 3). A 2×2 ANOVA attributed 99.2–99.4% of explained variance to patchification, while augmentation and interaction effects each contributed less than 0.1%, suggesting that performance gains were driven mainly by scale rescaling rather than sample count. On patchified data, fine-tuned models converged to 85.5–86.4% mAP50. YOLOv11n-Seg achieved the highest mAP50-95 of 51.1%, with 6× faster inference and 17× fewer parameters. In contrast, zero-shot Cellpose and StarDist reached only 45.3–51.2% class-agnostic F1@0.5. These findings show that structure-aware patchification is critical for reliable cell detection in this modality.
The Potential of Pasak Bumi (Eurycoma longifolia Jack) Root Extract Nanoparticles as Anti-Prostate Cancer Agent against Cell Cycle Regulation, Apoptosis, and Senescence based on In Vitro Tests Eka Yudha Rahman; Nia Kania; Didik Priyandoko; Adilah Hafizha Nur Sabrina; Wahyu Widowati; Rizal Azis; Aziz Annaba; Dhanar Septyawan Hadiprasetyo; Garry Alexandro
Journal of Mathematical and Fundamental Sciences Vol. 57 No. 3 (2026)
Publisher : Directorate for Research and Innovation (DRI) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2026.57.3.5

Abstract

Prostate cancer poses a major health challenge worldwide, with increasing incidence rates and limited current treatment modalities, which highlights the urgent need for innovative therapeutic alternatives. Utilizing nanoparticles derived from plant extracts is emerging as a promising solution, offering a superior drug delivery system. Eurycoma longifolia Jack roots, or Pasak Bumi, which are widely used in ethnobotany, are known to contain the compound eurycomanone, which exhibits pro-apoptotic activity against malignant cells. This investigation sought to assess the efficacy of Pasak Bumi root-derived nanoparticles (PBN) in inhibiting the proliferation of prostate carcinoma cells (PC-3), through an in vitro approach. A nanoparticle sample was obtained through the addition of chitosan and 0.4% Na-TPP to Pasak Bumi root extract. Flow cytometric analysis was employed to evaluate cell cycle distribution, while genes expression of Casp-3, Casp-8, and HAX-1 were quantified via qRT-PCR. The senescence cell detection test was performed using associated-β-galactosidase staining. PBN treatment supressed PC-3 cell proliferation through G0/G1 phase arrest and downregulation of HAX-1 genes expression, while promoting apoptosis via upregulation of Casp-3 and Casp-8 transcripts. PBN also increased the percentage of PC-3 senescence cells. This study demonstrates the potential of PBN to inhibit prostate cancer cells.
Mangosteen Peel Extract (Garcinia mangostana L.) as a Potential Complementary of Leukemia Therapy: In Vitro and In Silico Studies Marisca Evalina Gondokesumo; Arina Novilla; Sijani Prahastuti; Fadhilah Haifa Zahiroh; Hanna Sari Widya Kusuma; Wahyu Widowati; Rizal Azis; Dhanar Septyawan Hadiprasetyo; Aris Muhammad Nurjamil; Wahyu Surakusumah; Khoerotul Nur Fadhilah Adha
Journal of Mathematical and Fundamental Sciences Vol. 57 No. 2 (2025)
Publisher : Directorate for Research and Innovation (DRI) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2025.57.2.3

Abstract

Leukemia is caused by abnormal white blood cell proliferation that leads to disruption of conventional blood cell functions. Mangosteen (Garcinia mangostana L.) contains phytochemical compounds with strong anticancer properties, according to various cancer studies. This study investigated the potential of mangosteen peel extract (MPE) as an anti-leukemia agent through molecular docking of LC-MS-identified compounds against FLT-1 and AKT proteins, followed by in vitro evaluation on HL-60 cells, namely cytotoxic (WST-8 assay), ROS levels and senescence (flow cytometry), and then FLT1 and AKT gene expression (qRT-PCR). LC-MS identified α-mangostin, β-mangostin, γ-mangostin, mangostinone, and epicatechin as the main compounds. Molecular docking revealed strong binding affinities from −8.5 to −9.9 kcal/mol against AKT, and from −9.0 to −9.9 kcal/mol against FLT-1. MPE (500 µg/mL) decreased cell viability and increased inhibition of HL-60 cells. Intracellular ROS levels decreased significantly at 0.6, 1.2, and 2.4 µg/mL MPE. MPE induced cell senescence especially at 1.2 and 2.4 µg/mL. Gene expression analysis revealed downregulation of AKT at 1.2 and 2.4 µg/mL and FLT1 at 2.4 µg/mL. These findings suggest that MPE may exert multifactorial anti-leukemic mechanisms, including apoptosis, ROS modulation, senescence induction, and regulation of AKT and FLT1 expression.
Co-Authors Adilah Hafizha Nur Sabrina Ahmad Faried Alexandro, Garry Annaba, Aziz Arina Novilla Aris Muhammad Nurjamil Aziz Annaba Basari Basari Dhanar Septyawan Hadiprasetyo Dhanar Septyawan Hadiprasetyo Didik Priyandoko Didik Priyandoko Eka Yudha Rahman Eka Yudha Rahman Eltania, The Fransiska Fadhilah Haifa Zahiroh Garry Alexandro Gunanegara, Rimonta Febby Hadiprasetyo, Dhanar Septyawan Hanna Sari Widya Kusuma Hanna Sari Widya Kusuma, Hanna Sari Widya Harry Murti Jeffrey ., Jeffrey Khoerotul Nur Fadhilah Adha Marisca Evalina Gondokesumo Marisca Evalina Gondokesumo, Marisca Evalina Mars, Nicholas Mohammad Ikhsan Nainggolan, Ita Margaretha Nia Kania Nia Kania Nindya, Faradhina Salfa Qlintang, Sandy RAHMAT, DENI Sabrina, Adilah Hafizha Nur Sijani Prahastuti Teresa Liliana Wargasetia Triharsiwi, Dwi Nur Vinna Kurniawati Sugiaman, Vinna Kurniawati Wahyu Surakusumah Wahyu Widowati Wahyu Widowati WAHYU WIDOWATI Zino Ramdani Suharto