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Potensi Ekstrak Etanol Daun Suren (Toona Sinensis) Sebagai Insektisida Nabati dalam Pengendalian Hama Ulat Api (Setothosea Asigna V. Eecke) Harmileni, Harmileni
Agroprimatech Vol. 6 No. 1 (2022): Agroprimatech
Publisher : Prodi Agroteknologi Fakultas Agro Teknologi Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Salah satu hama yang terdapat pada perkebunan kelapa sawit adalah hama ulat api (Setothosea asigna v. Eecke), merupakan hama yang dapat menyebabkan kerusakan berat serta sangat merugikan perkebunan kelapa sawit. Untuk daerah tertentu, ulat api sudah menjadi endemik sehingga sangat sulit dikendalikan. Penelitian ini bertujuan untuk mengetahui potensi pemberian ekstrak daun suren sebagai insektisida nabati dalam mengendalikan hama ulat api (Setothosea asigna V. Eecke). Daun suren diekstrak menggunakan pelarut etanol 96% dengan cara maserasi, yang selanjutnya dilakukan penguapan menggunakan rotary evaporator sehingga didapatkan ekstrak pekat. Variasi konsentrasi ekstrak daun suren yang digunakan pada penelitian ini adalah 5%, 10%, 15%, 20%, 25% dan 30%. Metode yang dilakukan adalah metode penyemprotan langsung ekstrak daun suren ke ulat api yang ada pada bibit kelapa sawit dengan menggunakan Deltametrin sebagai pembanding. Hasil penelitian menunjukkan bahwa ekstrak daun suren dapat mengendalikan hama ulat api dengan rataan tercepat waktu kematian seluruh ulat api adalah 22 jam 3 menit pada konsentrasi 30%. Dari hasil penelitian ini menunjukkan bahwa ekstrak daun suren mempunyai aktivitas yang sangat baik dalam mengendalikan hama ulat api pada tanaman kelapa sawit. Kata kunci: Daun suren; hama ulat api (Setothosea asigna V. Eecke.); insektisida nabati
Hypoglycemic Activity and Safety Assessment of Pediococcus acidilactici Strain DNH16 in Experimental Type 2 Diabetes Mellitus Rats Induced with Streptozotocin Fachrial, Edy; Lina, Juliana; Harmileni, Harmileni; Anggraini, Sari; Sihotang, Widya Yanti
The Indonesian Biomedical Journal Vol 16, No 1 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i1.2781

Abstract

BACKGROUND: Type 2 diabetes mellitus (T2DM) cases tend to rise throughout the year in all parts of the world. The α-glucosidase inhibitors are frequently used to both prevent and treat T2DM. This α-glucosidase inhibitor activity is seen in some probiotic species, and a certain strain of Pediococcus acidilactici exhibits promising characteristics as an α-glucosidase inhibitor. This study was conducted to assess the hypoglycemic activity and safety of P. acidilactici strain DNH16 (PADNH16) in experimental rats with T2DM induced by streptozotocin.METHODS: The experiment was conducted in vivo using Wistar rats. Acarbose was employed as a positive control, and Lactobacillus casei was used as a comparative. For 24 days, post-prandial blood glucose levels were assessed every 3 days, followed by serum biochemical levels measurement of liver, kidney, and lipid profiles. The pancreas was histopathologically examined utilizing the Hematoxyline-Eosin staining procedure.RESULTS: Administration of PADNH16 to T2DM rats lowered post-prandial blood glucose levels and gave hypoglycemic benefits comparable to acarbose and commercial probiotics. PADNH16 dosing did not affect serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), urea, or creatinine levels, showing that PADNH16 was not hazardous to liver or kidney function. The lipid profile assessment revealed that the values, including high-density lipoprotein (HDL), low-density lipoprotein (LDL), and triglycerides, were comparable to the control group. The pancreatic histopathology revealed that injection of PADNH16 caused no alterations to pancreatic β cells.CONCLUSION: P. acidilactici isolate DNH16 has a hypoglycemic effect on T2DM rats, but does not affect liver function enzymes, kidneys, lipid profiles and does not provide significant changes in pancreatic β cells.KEYWORDS: diabetes mellitus, inhibitor α-glucosidase, Pediococcus acidilactici strain DNH16
Potensi Ekstrak Etanol Daun Suren (Toona Sinensis) Sebagai Insektisida Nabati dalam Pengendalian Hama Ulat Api (Setothosea Asigna V. Eecke) Harmileni, Harmileni
Agroprimatech Vol. 6 No. 1 (2022): Agroprimatech
Publisher : Prodi Agroteknologi Fakultas Agro Teknologi Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/agroprimatech.v6i1.2820

Abstract

Salah satu hama yang terdapat pada perkebunan kelapa sawit adalah hama ulat api (Setothosea asigna v. Eecke), merupakan hama yang dapat menyebabkan kerusakan berat serta sangat merugikan perkebunan kelapa sawit. Untuk daerah tertentu, ulat api sudah menjadi endemik sehingga sangat sulit dikendalikan. Penelitian ini bertujuan untuk mengetahui potensi pemberian ekstrak daun suren sebagai insektisida nabati dalam mengendalikan hama ulat api (Setothosea asigna V. Eecke). Daun suren diekstrak menggunakan pelarut etanol 96% dengan cara maserasi, yang selanjutnya dilakukan penguapan menggunakan rotary evaporator sehingga didapatkan ekstrak pekat. Variasi konsentrasi ekstrak daun suren yang digunakan pada penelitian ini adalah 5%, 10%, 15%, 20%, 25% dan 30%. Metode yang dilakukan adalah metode penyemprotan langsung ekstrak daun suren ke ulat api yang ada pada bibit kelapa sawit dengan menggunakan Deltametrin sebagai pembanding. Hasil penelitian menunjukkan bahwa ekstrak daun suren dapat mengendalikan hama ulat api dengan rataan tercepat waktu kematian seluruh ulat api adalah 22 jam 3 menit pada konsentrasi 30%. Dari hasil penelitian ini menunjukkan bahwa ekstrak daun suren mempunyai aktivitas yang sangat baik dalam mengendalikan hama ulat api pada tanaman kelapa sawit. Kata kunci: Daun suren; hama ulat api (Setothosea asigna V. Eecke.); insektisida nabati
IMPLEMENTASI INTERNET OF THINGS (IoT) DALAM SISTEM PENGONTROLAN SUHU OTOMATIS BERBASIS MIKROKONTROLER PADA ALAT PROOFER UNTUK PENGEMBANG ADONAN ROTI DI UMKM PIZZA OEI OEI AND PATISSERIE Nelson Silitonga; Dian Kurnia; Elvri Melliaty Sitinjak; Sari Farah Dina; Abdillah; Golfrid Gultom; Harmileni; Rycce Sylviana Pratikha; Yanto; Muhammad Iqbal Harapan Muslim Siregar
Journal of Golden Generation Abdimas Vol. 1 No. 2 (2025): Desember : Journal of Golden Generation Abdimas
Publisher : PT. Lembaga Penerbit Penelitian Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65244/jgga.v1i2.398

Abstract

Implementation of Internet of Things (IoT) technology and microcontrollers as an automatic temperature control system on tools for proofing bread dough at the Oei Oei Pizza and Patisserie Micro, Small, and Medium Enterprise (MSMEs). The primary issue faced by partners was temperature instability during the dough fermentation process, leading to inconsistent product quality. The implementation method included problem identification, tool design and construction, implementation at partner locations, and mentoring and evaluation. The results of the activity showed that the tool can help IoT-based systems maintain stable fermentation temperatures, improve dough quality consistency, and increase production efficiency. This initiative also has a positive impact on increasing partners' capacity to utilize appropriate technology.
Inhibisi Enzim α-Glukosidase oleh Bacillus Megaterium ITU 9 Isolat Sumber Air Panas Sumatera Utara Finna Piska; Chrismis Novalinda Ginting; Harmileni Harmileni; Edy Fachrial
Journal of Pharmascience Vol. 11 No. 1 (2024): Jurnal Pharmascience
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v11i1.17045

Abstract

Diabetes adalah penyakit multifaktor yang insidensinya meningkat dengan pesat di negara berkembang. Diabetes mellitus (DM) tipe 2 adalah jenis diabetes yang paling umum ditemui, dimana penyakit ini erat kaitannya dengan kemajuan industri dan teknologi sehingga mempengaruhi gaya hidup seseorang. Penanggulangan penyakit ini dapat dilakukan dengan menghambat aktivitas α-glukosidase. Beberapa mikroorganisme terutama dari sumber air panas dapat dimanfaatkan sebagai penghasil senyawa inhibitor α-glukosidase. Pada penelitian sebelumnya isolat Bacillus megaterium ITU 9 merupakan isolat yang diisolasi dari sumber air panas. Pada penelitian ini diuji kemampuan isolat Bacillus megaterium ITU 9 sebagai penghasil inhibitor α-glukosidase dengan metode sumur. Daya persen inhibisi supernatan B. megaterium ITU 9 terhadap kerja enzim α-glukosidase adalah sebesar 46,67%, sedangkan daya persen inhibisi acarbose yaitu 70%. Dalam hal ini B. megaterium ITU 9 termofilik memiliki daya inhibisi terhadap enzim α-glukosidase. Kata Kunci: Bakteri Termofilik, Inhibitor, Akarbose, Metode Sumur, Persen Inhibisi Diabetes is a multifactorial disease whose incidence is increasing rapidly in developing countries. Diabetes mellitus (DM) type 2 is the most common type of diabetes, where this disease is closely related to industrial and technological advances, thus affecting a person's lifestyle. This disease can be controlled by inhibiting α-glucosidase activity. Several microorganisms, especially from hot springs, can be used to produce α-glucosidase inhibitor compounds. In previous research, the Bacillus megaterium ITU 9 isolate was an isolate isolated from hot springs. In this study, the ability of the Bacillus megaterium ITU 9 isolate to produce α-glucosidase inhibitors was tested using the well method. The percent inhibition power of B. megaterium ITU 9 supernatant on the action of the α-glucosidase enzyme was 46.67%, while the percent inhibition power of acarbose was 70%. In this case, the thermophilic B. megaterium ITU 9 has inhibitory power against the α-glucosidase enzyme.
IMPLEMENTASI INTERNET OF THINGS (IoT) DALAM SISTEM PENGONTROLAN SUHU OTOMATIS BERBASIS MIKROKONTROLER PADA ALAT PROOFER UNTUK PENGEMBANG ADONAN ROTI DI UMKM PIZZA OEI OEI AND PATISSERIE Nelson Silitonga; Dian Kurnia; Elvri Melliaty Sitinjak; Sari Farah Dina; Abdillah; Golfrid Gultom; Harmileni; Rycce Sylviana Pratikha; Yanto; Muhammad Iqbal Harapan Muslim Siregar
Journal of Golden Generation Abdimas Vol. 1 No. 2 (2025): Desember : Journal of Golden Generation Abdimas
Publisher : PT. Lembaga Penerbit Penelitian Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65244/jgga.v1i2.398

Abstract

Implementation of Internet of Things (IoT) technology and microcontrollers as an automatic temperature control system on tools for proofing bread dough at the Oei Oei Pizza and Patisserie Micro, Small, and Medium Enterprise (MSMEs). The primary issue faced by partners was temperature instability during the dough fermentation process, leading to inconsistent product quality. The implementation method included problem identification, tool design and construction, implementation at partner locations, and mentoring and evaluation. The results of the activity showed that the tool can help IoT-based systems maintain stable fermentation temperatures, improve dough quality consistency, and increase production efficiency. This initiative also has a positive impact on increasing partners' capacity to utilize appropriate technology.
Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia Edy Fachrial; Sari Anggraini; Harmileni; Titania T Nugroho; Saryono
Biodiversitas Journal of Biological Diversity Vol. 20 No. 12 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201205

Abstract

Abstract. Fachrial E, Anggraini S, Harmileni, Nugroho TT, Saryono. 2019. Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia. Biodiversitas 20: 3493-3498. The application of enzymes industrially is increasing every year and thermophilic microbes are a promising source of these enzymes for industrial use due to their temperature stability. The aim of this study, therefore, was to isolate, characterize and identify the enzyme producer-thermophilic bacteria from the Penen Hot Spring in Deli Serdang, North Sumatra, Indonesia. In the experimental setup, carbohydrase activities including inulinase and amylase were determined by the formation of clear zone around the colonies after soaking with Lugol on the TSA medium supplemented with 1% inulin powder and 1% starch. Similarly, the protease activities were determined by the formation of clear zone around the colonies on Skim Milk Agar Medium. Then, of the 11 isolates, only one known as UTMP 12 showed maximum enzyme activity. The isolate was then characterized based on morphology and biochemistry and found to be Bacillus subtilis strain JCM 1465 (accession number NR_113265, homolog 99.72%). Furthermore, this is the first study on carbohydrase and protease activities of Bacillus subtilis strain JCM 1465 and the result shows that the thermophilic bacteria are needed in the production of carbohydrase and protease.
Molecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia Edy Fachrial; Raden Roro Jenny Satyo Putri; I Nyoman Ehrich Lister; Sari Anggraini; Harmileni HARMILENI; Titania T. Nugroho; Saryono SARYONO
Biodiversitas Journal of Biological Diversity Vol. 21 No. 10 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d211035

Abstract

Abstract. Fachrial E, Putri RRJS, Lister INE, Anggraini S, Harmileni, Nugroho TT, Saryono. 2020. Molecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia. Biodiversitas 21: 4719-4725. The industrial need for stable microbial enzymes tends to increase every year. The aim of this study was to isolate and identify the protease and cellulase producing thermophilic bacteria isolated from the geothermal spring of Lau Sidebuk Debuk, North Sumatra, Indonesia. The protease activity was determined based on the formation of halo zone on Skim Milk Agar, while cellulase was confirmed using CMC plate agar method. Nine bacterial isolates were successfully obtained from the sediments and water, and both activities were observed in only one isolate, known as UTMSA14. These isolates were characterized biochemically and morphologically. The molecular identification of UTMSA14 was done by 16S rRNA sequencing based on BLAST and phylogenetic analysis using MEGA X. Phylogenetic analysis showed that UTMSA14 clustered together with Bacillus tequilensis strain 10b (accession number NR_104919.1), which ascertained the isolate as Bacillus tequilensis strain 10b (accession number NR_104919.1). This is the first report of a thermophilic bacteria isolated from Lau Sidebuk Debuk hot springs, with the ability to produce cellulase and protease. further research is needed to purify and characterize the resulting enzymes, with a potential for industrial applications.
Isolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from Dolok Tinggi Raja Natural Hot Springs, North Sumatra, Indonesia Edy Fachrial; VISENSIUS KRISDIANILO; Harmileni HARMILENI; I Nyoman Ehrich Lister; Titania T Nugroho; Saryono SARYONO
Biodiversitas Journal of Biological Diversity Vol. 22 No. 4 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220416

Abstract

Abstract. Fachrial E, Krisdianilo V, Harmileni, Lister INE, Nugroho TT, Saryono. 2021. Isolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from Dolok Tinggi Raja Natural Hot Springs, North Sumatra, Indonesia. Biodiversitas 22: 1725-1732. Proteases are complex enzymes that can be applied in both commercial products and physiological fields. This enzyme accelerates the breakdown of peptide bonds in polypeptides and proteins using hydrolysis reactions, transforming them into simpler bonds. In recent years, the demand for protease enzymes in Indonesia is still dependent on imported products, and there has also been an increase in their use. This is because there are many advantages in terms of efficiency, environmental friendliness, and its ability to catalyze a reaction without by-products. Proteases dominate the enzyme market with a total of 70% sales. Furthermore, they are manufactured from thermophilic bacteria as a solution to the problem of enzyme use in industries, where enzymes are easily damaged at high temperatures. The thermophilic protease enzyme can be found in thermophilic microorganisms living in extreme temperatures such as hot springs. There are lots of mountainous areas in North Sumatra Province, Indonesia with many hot springs. For example, the Dolok Tinggi Raja Natural Hot Springs of Simalungun District which has a water temperature of 50oC with a pH of about 6, the potential of which still needs to be explored. This study aims to isolate and determine the activity and molecular structure of thermophilic bacteria producing protease in Dolok Tinggi Raja Natural Hot Springs. Protease activity was determined based on the formation of clear zones in Skim Milk Agar medium. In addition, a total of 20 isolates were successfully obtained from sediment and water. Each was tested biochemically and morphologically, where 19 isolates had the activity of proteases, with the highest in isolate UTMTR VK S9 of 23.67 U/mL. Molecular identification was performed by the amplification of 16SrRNA gene based on BLAST and phylogenic analysis with MEGA X. Phylogenic results showed that isolate UTMTR VK S9 possessed similarities to Bacillus paralicheniformis strain A30103. Meanwhile, more research is required to purify enzymes for application in the industrial world.
Molecular identification of thermophilic bacteria with antimicrobial activity isolated from hot springs in North Sumatra, Indonesia CHRISMIS NOVALINDA GINTING; FINNA PISKA; HARMILENI HARMILENI; EDY FACHRIAL
Biodiversitas Journal of Biological Diversity Vol. 24 No. 2 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240210

Abstract

Abstract. Ginting CN, Piska F, Harmileni, Fachrial E. 2023. Molecular identification of thermophilic bacteria with antimicrobial activity isolated from hot springs in North Sumatra, Indonesia. Biodiversitas 24: 752-758. Microorganisms such as thermophilic bacteria are promising sources of antimicrobial compounds due to their abundance, which has yet to be widely investigated. Therefore, this study aims to identify thermophilic bacteria isolated from natural hot springs, Sidebuk Debuk, in North Sumatra, Indonesia. The bacteria were isolated from sediments and hot springs water. Isolated 10 bacteria were characterized and identified based on their morphological and biochemical properties, which included gelatinase activity, motility, citrate utilization, triple sugar iron agar test, catalase test, cell shape, color, and Gram staining. Antimicrobial activity was tested using the disc diffusion method against Escherichia coli and Staphylococcus aureus. The most potential isolates were identified molecularly by amplification of 16S rRNA genes using universal primers. The sequences result were trimmed and assembled using BioEdit and submitted to BLAST to determine the homology of the bacteria. The isolate ITU9 was identified as Bacillus megaterium and exhibited the best antimicrobial activity with an inhibition zone of 9.5 mm against S. aureus during 72 h of incubation. This research showed that the thermophilic B. megaterium strain ITU9 was successfully isolated from Sidebuk Debuk hot springs and had stable antimicrobial activity for 72 h against E. coli and S. aureus.
Co-Authors Abdillah Abdillah Agus Kembaren Ahmad Nasir Pulungan Aloysius Aloysius Aloysius, Aloysius Anggita Kasih Fianti Situmorang Anggraini, Sari Anjurniza Ulfa Ary Anggara Wibowo Benny Rio Fernandez Chiuman, Linda CHRISMIS NOVALINDA GINTING Chrismis Novalinda Ginting Dian Kurnia Dian Kurnia EDY FACHRIAL EDY FACHRIAL Edy Fachrial Elvri Melliaty Sitinjak FINNA PISKA Finna Piska Ginting, Chrismis Novalinda Gultom, Golfrid Herlinawati Herlinawati I Nyoman Ehrich Lister I Nyoman Ehrich Lister Junifa Layla Sihombing Lina, Juliana Marfitania, Taranipa Meutia Mirnandaulia Muhammad Iqbal Harapan Muslim Siregar Nelson Silitonga Nelson Silitonga Raden Roro Jenny Satyo Putri Rahayu Rahayu Rama Riana Sitinjak Rika Silvany Rycce Sylviana Pratikha Rycce Sylviana Pratikha Saragih, Gimelliya SARI ANGGRAINI Sari Anggraini Sari Anggraini Sari Farah Dina Sari Farah Dina Saryono Saryono SARYONO Saryono SARYONO SARYONO SARYONO Sitinjak, Elvri Melliaty Situmorang, Anggita Kasih Fianti Supriyadi Tengku Rachmi Hidayani Tengku Rachmi Hidayani, Tengku Rachmi Titania T Nugroho Titania T Nugroho TITANIA T. NUGROHO Titania T. Nugroho Ulfa, Anjurniza VISENSIUS KRISDIANILO Widya Yanti Sihotang Yanto YANTO Yunianto Yunianto