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All Journal Meditory : The Journal of Medical Laboratory Jurnal Analis Kesehatan Jurnal Riset Kesehatan Poltekkes Depkes Bandung JMLS: Journal of Medical Laboratory and Science Jurnal Kesehatan Siliwangi Jurnal Pengabdian Masyarakat Kesehatan Indonesia Jurnal Media Analis Kesehatan Jurnal Info Kesehatan
Noviar, Ganjar
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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THE EFFECT OF TEMPERATURE AND STORAGE DURATION OF WASHED RED CELLS ON THE NUMBER OF ERYTHOCYTES Andina, Chika Alivia; Noviar, Ganjar; Marliana, Nina; Hayati, Eem
Meditory : The Journal of Medical Laboratory Vol 12, No 2 (2024): Meditory, Volume 12 No. 2 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i2.3428

Abstract

Background: Blood services in Indonesia, including processing and storing donor blood, aim to ensure blood quality remains optimal. Washed Red Cell blood components are used for patients with severe allergies due to repeated transfusions, have a limited shelf life and are susceptible to secondary infections. Aims: Blood services in Indonesia, including processing and storing donor blood, aim to ensure blood quality remains optimal. Washed Red Cell blood components are used for patients with severe allergies due to repeated transfusions, have a limited shelf life and are susceptible to secondary infections. Methods: This research design uses a quasi-experiment with serial time measurements. Samples were taken from one bag of WRC donor blood from the Bandung City PMI Blood Transfusion Unit. Specimens were stored at room temperature (20-25°C) and refrigerator temperature (4-6°C) for 0, 3, 6, and 9 hours, and the number of erythrocytes was measured using a Haematology Analyzer. The results of the study showed that the average number of erythrocytes stored at room temperature was 7.54×10⁶/µL at the 0 hour examination, 6.37×10⁶/µL at the 3 hour examination, 5.40×10⁶/µL at the 6 hour examination. and at the 9 hour examination 4.56×10⁶/µL. Meanwhile, the average number of erythrocytes stored at refrigerator temperature was at the 0 hour examination 7.65×10⁶/µL, at the 3 hour examination 7.43×10⁶/µL, at the 6 hour examination 7.25×10⁶/µL, and at 9 hour examination 7.02×10⁶/µL. The results of the General Linear Model (GLM) statistical test show a sig value = 0.000 0.05. Conclusions: temperature and storage time have a significant influence on the number of erythrocytes.
THE EFFECT OF EDTA BLOOD STORAGE TIME AND ROOM TEMPERATURE ON THE EXAMINATION OF ERYTHROCYTE SEDIMENTATION RATE Nabila, Lazulpa; Durachim, Adang; Hayati, Eem; Noviar, Ganjar
Meditory : The Journal of Medical Laboratory Vol 12, No 2 (2024): Meditory, Volume 12 No. 2 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i2.3517

Abstract

Background: Erythrocyte Sedimentation Rate (ESR) is an examination that aims to determine the speed of erythrocytes settling in the blood measured for 1 hour. The examination of ESR performed within 2 hours at most. However, the examination is often not performed immediately due to several reasons that cause delays for several hours. The room temperature during the examination affects the examination. The temperature in West Java has the highest average reaching 32°C. Aims: To determine an effect of EDTA blood storage time and room temperature on the examination of ESR. Method: This research is included in the type of quasi eksperimental research by involving variations in blood storage time immediately, 4 hours, and 5 hours and room temperature during examination is room temperature 20-25oC and temperature 29-32oC. Result: The result of this study showed that there was a significant effect of the variable EDTA blood storage time and room temperature on the examination of ESR which is indicated by the value of Sig. 0,00 (Sig. 0,05). This is because EDTA blood stored more than 2 hours after blood collection will affect the shape of the cells and the temperature can affect the settling speed. Conclusion: Based on the results of statistical tests, it can be concluded that there is an effect of EDTA blood storage time and room temperature on the examination of ESR
Persistence of Antibody Response Against SARS-CoV-2 After Vaccination Bekti, Heri Setiyo; Habibah, Nur; Dharmawati, I Gusti Agung Ayu; Merdekawati, Fusvita; Noviar, Ganjar
JURNAL INFO KESEHATAN Vol 21 No 4 (2023): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol21.Iss4.1066

Abstract

SARS-CoV-2 is the causative agent of the disease known as COVID-19. COVID-19 is spreading very fast around the world. One of the immune responses that play a role in against SARS-CoV-2 infection is the production of antibodies, which is 3 weeks after infection. Where within 3 weeks after infection, antibodies will be produced against RBD and the S1 and S2 domains in glycoprotein S and nucleocapsid protein N. The ability of an antibody to inhibit viral infection is determined by its level or titer. This study aims to determine the description of antibody levels against SARS-CoV-2 after vaccination. This type of research is descriptive research. Measurement of antibody levels for SRBD SARS-CoV-2 was carried out using the CLIA method using the MAGLUMI tool. Of the 30 respondents, 23 people had received the third vaccine. The results of this study showed that the average level of SRBD antibodies against SARS-CoV-2 in respondents with 2 doses of vaccine (1.063,786 BAU/mL) was higher than in respondents with 3 doses of vaccine (535.651 BAU/mL). Vaccine intervals of more than 6 months (908.338 BAU/mL) have higher antibody levels than respondents with vaccine intervals of 1-6 months (228.006 BAU/mL). The conclusion of this study is the highest antibody titers are produced >6 months after vaccination, antibody titers are still detectable after 12 months of vaccination, and for further research, it can be measured antibody levels against SARS-CoV-2 from people who have got vaccination for a duration of 2 years or more.
Pengaruh Suhu dan Lama Simpan Washed Red Cell Terhadap Nilai Fragilitas Osmotik Metode Spektrofotometri A, Nisa; Nurhayati, Betty; Noviar, Ganjar; Durachim, Adang
Jurnal Media Analis Kesehatan Vol 16 No 2 (2025): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v16i2.1682

Abstract

Washed Red Cell adalah produk darah yang diperoleh dari proses pencucian Packed Red Cell, di mana zat aditifnya dihilangkan, sehingga lebih mudah mengalami hemolisis. Hemolisis saat penyimpanan bisa dipicu oleh faktor suhu dan lama penyimpanan. Penelitian ini ditujukan guna mengkaji pengaruh suhu serta lama penyimpanan terhadap tingkat fragilitas osmotik pada Washed Red Cell menggunakan metode spektrofotometri. Penelitian ini menggunakan rancangan kuasi-eksperimen, dengan satu unit kantong Washed Red Cell bergolongan darah O Rh+ yang ditempatkan pada kondisi suhu lemari pendingin dan suhu lingkungan selama 0, 4, 7, serta 10 jam. Hasil telaah deskriptif menunjukkan adanya kenaikan tingkat fragilitas osmotik pada seluruh kelompok perlakuan. Uji Friedman menunjukkan bahwa suhu penyimpanan refrigerator dengan ruang dan durasi penyimpanan (0, 4, 7, dan 10 jam) berpengaruh signifikan pada nilai fragilitas osmotik, dengan nilai signifikansi kurang dari 0,001 (P<0,05). Uji Wilcoxon juga mengonfirmasi adanya pengaruh lama penyimpanan, di mana sampel yang disimpan segera berbeda signifikan dengan yang ditunda selama 4, 7, dan 10 jam, dengan nilai signifikansi tidak melebihi 0,001 (P<0,05). Selain itu, uji Spearman menunjukkan hubungan yang kuat antara suhu penyimpanan dan lama penyimpanan dengan nilai fragilitas osmotik, dengan nilai signifikansi kurang dari 0,001 (P<0,05). Hubungan ini bersifat positif, ini berarti makin tingginya suhu dan makin lamanya waktu penyimpanan, maka nilai fragilitas osmotik pun akan semakin meningkat.
Co-Authors A, Nisa Andina, Chika Alivia Arbie, Arbie Dewi Nurhayati Durachim, Adang Eka Setiawan, Regina Hamidah, Hani Hayati, Eem Heri Setiyo Bekti HUTAPEA, BETARIA ANGGELINA I Gusti Agung Ayu Dharmawati Ihsan Nurhadi, Muhammad Kurniawan , Entuy Lestari, Felia Marliana , Nina Merdekawati, Fusvita Nabila, Lazulpa Nina Marliana, Nina Nur Habibah Nur Ramadhani, Riski Nurhayati, Betty Rahayu, Ira Gustira Rahmat , Mamat Ramadhani, Riski Nur Riyani, Ani Rohayati Rosya, Reni Apriani Suhartini, Entin Suhartini, Rina Titin Hartini Wardhani, Azkya Zhahira Wiryanti, Wiwin