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Journal : International Journal of Renewable Energy Development

Comparative Study on the Various Hydrolysis and Fermentation Methods of Chlorella vulgaris Biomass for the Production of Bioethanol Megawati, Megawati; Bahlawan, Zuhriyan Ash Shiddieqy; Damayanti, Astrilia; Putri, Radenrara Dewi Artanti; Triwibowo, Bayu; Prasetiawan, Haniif
International Journal of Renewable Energy Development Vol 11, No 2 (2022): May 2022
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.2022.41696

Abstract

One of the microalgae that can be potentially used to produce bioethanol is Chlorella vulgaris, as it is rich in carbohydrates. However, the carbohydrates in C. vulgaris cannot be converted directly into ethanol. This study aimed to investigate the chemical and enzymatic hydrolysis of C. vulgaris, which is subsequently followed by fermentation. The catalysts used in the chemical hydrolysis were hydrochloric acid, sodium hydroxide, and potassium hydroxide, while the enzymes used were the mixture of alpha-amylase + glucoamylase, alpha-amylase + cellulase, and alpha-amylase + glucoamylase + cellulase. The hydrolysate obtained from chemical hydrolysis was fermented through Separate Hydrolysis Fermentation (SHF), while the one from enzymatic hydrolysis was fermented through Simultaneous Saccharification and Fermentation (SSF), in which both processes used S. cerevisiae. After undergoing five hours of enzymatic hydrolysis (using alpha-amylase + glucoamylase), the maximum glucose concentration obtained was 9.24 ± 0.240 g/L or yield of 81.39%.  At the same time and conditions of the substrate on chemical hydrolysis, glucose concentration was obtained up to 9.23 + 0.218 g/L with a yield of 73.39% using 1 M hydrochloric acid. These results indicate that chemical hydrolysis is less effective compared to enzymatic hydrolysis. Furthermore, after 48 hours of fermentation, the ethanol produced from SHF and SSF fermentation methods were 4.42 and 4.67 g/L, respectively, implying that producing bioethanol using the SSF is more effective than the SHF method.
Co-Authors Abdurrafi, Faishol Achmad Wikandaru Akhmad Sutrisno Alfiansyah Aji Pratama Amrullah, Fauzan Anggun Septiamurti Anggun Septiamurti Astrilia Damayanti Astrilia Damayanti Ayu Nur Permadhini Bayu Triwibowo Bayu Triwibowo Catur Rini Widyastuti, Catur Rini Daffa Nur Muhammad Damayanti, Astrilia - Destantri, Fitri Wahyuningtyas Dewi, Yasinda Verrel Dinara Shinka Dhoni Hartanto Diana, Savira Rahma Dillah, Shohib Qomad Dwi Prasojo Dyta Silvia Mayasari Fahreza Pracenda Felicitia Fandika, Putri Maharani Adinda Fauzan Amrullah Handoko, Pratama Senapati Bagus Hanif Ardhiansyah Haniif Prasetiawan Harianingsih, Harianingsih Hutomo , Gayuh Aditya Hutomo, Gayuh Aditya Ichwan, Rizky Imani, Nadya Alfa Cahaya Imanullah, Kautsar Taqi Irene Nindita Pradnya Jannah, Reni Ainun Kakalia Putri Auralita Karenina Hakim Khoridatus Sulwa Kristi, Silvia Yolanda Maharani Kusumaningrum Maulida Zakia Mayasari, Dyta Silvia Megawati Megawati - Megawati Megawati Megawati Megawati Megawati Megawati Megawati Megawati Muna, Mochammad Choiril Nabil, Nisrina Hasna’ Ninda Kurniadi Nurul Khusna Permanadewi, Indrasukma Pradiza, Zefanya Pradnya, Irene Nindita Pramitha, Deva Aurelya Prasetyo, Ridwan Anung Prasojo, Dwi Pratama, Alfiansyah Aji Pratama, Muhammad Herdi Pratiwi, Kharisma Ardhani Nur Prima Astuti Handayani Rachmadi, Moch Faizal Radenrara Dewi Artanti Putri Radenrara Dewi Artanti Putri Radenrara Dewi Artanti Putri, Radenrara Dewi Artanti Ratna Dewi Kusumaningtyas Ria Wulansarie, Ria S., Syifa Azzahra Putri Sadan, Serlia Fitri Salsabila, Luvena Septiamurti , Anggun Serlia Fitri Sadan Suwandi, Luluk Arvi Cahyaning Syahputra, Adhika Bintang Waliyuddin Sammadikun Wara Dyah Pita Rengga Wati, Afifah Sagita Fitria Zain, Faatin Nisriinaa Zefanya Pradiza