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All Journal PENA SAINS Science and Technology Indonesia Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) Jurnal Kimia Terapan Indonesia Fullerene Journal of Chemistry Stannum : Jurnal Sains dan Terapan Kimia Jurnal Sains Indonesia AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Bangun Desa : Jurnal Pengabdian Masyarakat
Muhammad Abdurrahman Munir
Universitas Alma Alta
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Comparison of real time PCR and conventional PCR by identifying genomic DNA of bovine and porcine Muhammad Abdurrahman Munir; Ahlam Inayatullah
Jurnal Kimia Terapan Indonesia Vol 23, No 2 (2021): Jurnal Kimia Terapan Indonesia
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/inajac.v23i2.491

Abstract

Bovine and porcine are poultry meat that consumed worldwide particularly in Southeast Asia.Both of them are prone to food counterfeit owing to several factors such as price, appetite and Halal status. Sensitive and selective analytical methods are required to control meat products that distributed to markets. This paper studied the sensitivity between real – time and conventional PCR or known as qPCR and cPCR, respectively. Bovine and porcine were samples used to verify the sensitivity of them. Nevertheless, those instruments did not show a specific difference during DNA analysis of bovine and porcine. In conventional PCR, two pairs of DNA primers targeted cytochrome b (Cyt b) was analyzed, resulting of 120 and 131 amplicons, respectively. While qPCR applied to analyze porcine and bovine DNA. The detection limit of qPCR after porcine and bovine analysis were at 0.004 and 0.007 µg/µL, respectively. Results demonstrated the qPCR was reliable for verifying porcine and bovine DNA compared to conventional PCR. Furthermore, the study concluded that the developed assay can be easily employed for the identification of porcine and bovine tissue in food products in low resource areas.
Biogenic Amines Detection by Chromatography and Sensor Methods: A Comparative Review Muhammad Abdurrahman Munir; Khairiah Haji Badri; Lee Yook Heng
Science and Technology Indonesia Vol. 5 No. 4 (2020): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3295.18 KB) | DOI: 10.26554/sti.2020.5.4.90-110

Abstract

Biogenic amines (BA) are chemistry compounds shaped by amino acids decarboxylation and exist in protein food and beverages. They are labelled toxic if consumed and some countries prohibit to consume them in high level especially histamine. Two major methods have been used and developed well such as chromatography methods and sensors methods. The common method applied for chromatography namely liquid chromatography (LC) and gas chromatography (GC) while for sensor methods such as optical, chemical and bio sensor. These methods have advantages and disadvantages. For chromatography methods, derivatization methods are required in order to improve their sensitivity and selectivity, nevertheless these methods are very expensive and time-consuming. During derivatization step, it needs more time and bear the risk of an only partial detection due to an incomplete derivatization. Furthermore, sensor methods exist to solve these issues, while they do not require derivatization step, generate a direct signal that can be interpreted by anyone, very fast and simple, yet they have disadvantages in several aspects such as sensitivity, accuracy and selectivity compared to chromatography methods. This review is based on studies about biogenic amines detection from the last decades until now and related to food and beverage samples. Although biogenic amines commonly found in protein-food for decades, new approaches and technical possibilities still required in order to increase the sensitivity, selectivity and accuracy of analytical methods to tackle the complexity by their matrices. The rationale of this study is also to provide data about the comparison of the analytical techniques between conventional and sensor methods. Furthermore, the various approaches of biogenic amines determination and the most applied analytical methods have been reviewed.
Fish Analysis Containing Biogenic Amines Using Gas Chromatography Equipped With Flame Ionization And Mass Spectrometer Detectors Muhammad Abdurrahman Munir; Ahlam Inayatullah; Hamid Alkhair Badrul
Science and Technology Indonesia Vol. 6 No. 1 (2021): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1073.894 KB) | DOI: 10.26554/sti.2021.6.1.1-7

Abstract

Biogenic amines generally can be found in fish due to amines in fish undergoing a degradation process. According to the United States Food and Drug Administration (FDA), biogenic amines in fish and fish products can cause harm to consumers if consumed more than 50 µg/mL. Thus, it is important to analyze them. Five biogenic amines such as heptylamine, histamine, tyramine, cadaverine and spermidine were extracted using soaking method with methanol 50% (v/v), afterward they were detected in fish and fish products using gas chromatography – flame ionisation detector (GC-FID) and the biogenic amines structures were confirmed using mass spectrometry (MS). The detection limits (DLs) were range at 1.20 – 2.90 µg/mL. Histamine was detected in fish and fish products such as sardine (Sardinella gibosa) and mackerel (Scomberomorus guttatus) at concentration of 5.96 and 2.69 µg/mL, respectively, whereas cadaverine was found in sardine (Sardinella gibosa) at concentration of 4.96 µg/mL. Histamine concentrations in this study were detected below 50 µg/mL which is below the permissible threshold associated with scombroid poisoing.
DETERMINATION OF ASCORBIC ACID CONCENTRATION IN MYRTACEAE USING THE IODOMETRIC TITRATION METHOD Nur Syafitri; Muhammad Abdurrahman Munir; Veriani Aprilia; Emelda Emelda
JURNAL PENA SAINS Vol 10, No 1 (2023): Jurnal Pena Sains
Publisher : Program Studi Pendidikan IPA, Fakultas Ilmu Pendidikan, Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/jps.v10i1.19161

Abstract

Vitamins are essential compounds in food even though the content contained in these foods is small, and this is because vitamins have a vital role in the human body. Vitamin C or ascorbic acid is a secondary antioxidant compound that captures free radical compounds. Vitamin C content is easily found in vegetables and fruits. One of the fruits that contain vitamin C is guava. This study aims to determine and analyze vitamin C concentrations in several guava fruit types with the iodometric titration method. This type of research is a quantitative-qualitative experimental laboratory. The samples used in this study were guava in Myrtaceae family consisting of red guava (Psidium guajava L.), crystal guava (Psidium guajava L.), and red water guava (Syzygium aqueum). The study began with making 0.05 N iodine, iodine formation, then the 3% amylum indicators. The average sample content of red guava, crystal guava, and red water guava was 2.42 mg/100 grams. To conclude, the ascorbic acid concentration of red guava water is higher at 157 mg/100 g compared to the other two guavas, which are 80 mg/100 g and 87 mg/100 g.
Penguatan Literasi Membaca, Menulis Dan Minat Belajar Matematika Anak Sekolah Dasar Di Dusun Santan Tahun 2022 Alfiana Deviana; Rachmad Bagas Yahya supriyono; Audia N Rumbaremata; Chairunisa Ta Marsaoly; Cahya Retna Ningtyas; Fera Erika Febranti; Ika Amalia Putri; Joni Yoga Pratama; Nailus Syafa’ah; Nunung Ismawati; Nur Hidayati; Yahya Supriyono; Ristia Laura Amanda; Siti Miftahus Sa’adah; Yuanita Sholihah; Muhammad Abdurrahman Munir
Bangun Desa: Jurnal Pengabdian Masyarakat Vol 1, No 2 (2022)
Publisher : Universitas Alma Ata

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (506.644 KB) | DOI: 10.21927/jbd.2022.1(2).51-54

Abstract

Penelitian ini bertujuan untuk meningkatkan kualitas belajar literasi membaca, menulis dan minat belajar matematika. Bimbingan belajar yang dilakukan di Dusun Santan merupakan kegiatan yang di laksanakan untuk meningkatkan kualitas sumber daya manusia sehingga anak peserta didik di Dusun Santan memahami cara membaca, menulis dan minat belajar matematika yang dilakukan di pendopo Dusun Santan. Dengan begitu kemampuan anak peserta didik di Dusun Santan akan semakin lancar dalam membaca, menulis dan minat dalam belajar matematika.Kata Kunci: Penguatan, literasi, santan
DETERMINATION OF VITAMIN C IN SPINACH (Amaranthus sp.) USING TITRATION METHOD Allefeti Sandrika; Muhammad Abdurrahman Munir; Veriani Aprilia; Emelda Emelda
JURNAL PENA SAINS Vol 10, No 2 (2023): Jurnal Pena Sains
Publisher : Program Studi Pendidikan IPA, Fakultas Ilmu Pendidikan, Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/jps.v10i2.19172

Abstract

Vitamin C is a compound that is included in the group of water-soluble vitamins. The human body really needs vitamin C intake because it can act as an antioxidant which is closely related to collagen formation and iron absorption. Vitamin C is an organic compound that the human body needs in small amounts to maintain the body metabolic functions. Vitamin C is also one of the most important vitamins in increasing the body immunity This compound is very easy to find in fruits and vegetables, one of which is spinach. Spinach is a vegetable which generally contain vitamin C with an average of 158.54 mg/100 g. The research aims to analyze vitamin C levels in spinach using a titration method or also known as iodometric titration. This research method is included in an experimental study. The sample used in this research was obtained from Kotagede Market, Yogyakarta. The titration results for each spinach will be analyzed using Microsoft Excel, where the results have shown that the levels of vitamin C contained in snapper spinach (18.95 mg/100 g), picked spinach (46.39 mg/100 g), thorn spinach (14.52 mg /100 g) and red spinach (15.42 mg/100 g). Therefore, it can be concluded that this titration method can be used to accurately measure the vitamin C content in spinach. The iodometric titration method was chosen to analyze vitamin C levels because this application is very simple when compared to other methods. Ascorbic acid or vitamin C is known as a strong reducing agent and can simply be titrated using iodine solution.
Aplikasi Metode Titrasi Iodometri Untuk Determinasi Kadar Vitamin C Pada Jambu (Myrtaceae Family Muhammad Abdurrahman Munir; Nur Syafitri; Veriani Aprilia; Emelda Emelda
Jurnal Kimia Fullerene Vol 8 No 2 (2023): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v8i2.492

Abstract

Vitamin merupakan senyawa penting di dalam makanan walaupun kandungan yang terdapat di dalam makanan tersebut sedikit, hal ini disebabkan vitamin memiliki peranan vital di dalam tubuh manusia. Vitamin dapat dikelompokan dalam 2 golongan yaitu vitamin yang larut di dalam lemak yaitu A, D, E, F dan K, sedangkan untuk vitamin yang larut dalam air diantaranya adalah vitamin C dan vitamin B. Vitamin C merupakan senyawa antioksidan sekunder yang bekerja dengan cara menangkap senyawa radikal bebas. Kandungan vitamin C mudah ditemukan di dalam sayur-sayuran dan buah-buahan. Salah satu buah-buahan yang mengandungi vitamin C adalah buah jambu (Myrtaceae family). Penelitian ini bertujuan untuk mengetahui dan menganalisis kadar vitamin C yang terkandung dalam beberapa jenis buah jambu (Myrtaceae family). Metode yang digunakan adalah metode titrasi iodometri. Jenis penelitian ini yaitu kuantitatif-kualitatif eksperimental laboratorium. Sampel yang digunakan dalam penelitian ini yaitu jambu biji merah (Psidium guajava L.), jambu kristal (Psidium guajava, L.), dan jambu air merah (Syzygium aqueum). Penelitian diawali pembuatan iodium 0,05 N, pembakuan iodium, kemudian pembuatan indikator amilum 3%. Selanjutnya penetapan kadar vitamin C pada sampel jambu menggunakan metode titrasi iodometri. Rata-rata kadar sampel jambu biji merah, jambu kristal, dan jambu air merah 2,42 mg/100 gram.
Review: A Modest Approach of Electrochemical Sensor to Determine Biogenic Amines in Food and Beverages Muhammad Abdurrahman Munir; Badri, Khairiah Haji; Heng, Lee Yook; Inayatullah, Ahlam; Badrul, Hamid Alkhair
Jurnal Sains Indonesia Vol 1 No 3 (2020): Volume 1, Nomor 3, 2020 (November)
Publisher : PUSAT SAINS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59897/jsi.v1i3.25

Abstract

Biogenic amines are chemical compounds that can be found in protein food. The analysis of biogenic amines in food samples is very important because the toxicity released by them is very adverse if consumed by human. Several methods have been applied and developed in order to detect biogenic amines such as liquid chromatography (LC) and gas chromatography (GC). Nevertheless, both of them are time consuming and using many chemical compounds. Thus, electrochemical sensors become the best solution in order to solve the issues from chromatography methods. Electrochemical sensors are very simple, easy to use and cheap. This paper reviews about various techniques of electrochemical sensor such as chemical sensors, biosensors and optical sensors that have been used for biogenic amines detection.
Analysis of total phenolic content and antioxidant activity of mahogany seed infusion (Swietenia mahagoni (L.) Jacq.) Pratiwi, Sindi; Emelda, Emelda; Kusumawardani, Nurul; Munir, Muhammad Abdurrahman; Azizah, Abibatul; Pothitirat, Werayut
Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) VOLUME 12 ISSUE 4, 2024
Publisher : Alma Ata University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21927/ijnd.2024.12(4).262-272

Abstract

Background: The mahogany plant (Swietenia mahagoni) is a plant used in medicine. Mahogany seeds are also efficacious as antiseptics, antioxidants, and antimicrobials. This plant contains flavonoids, saponins, tannins, essential oils, alkaloids, and anthraquinones. The higher the phenolic content in a plant, the higher the antioxidant activity. According to previous studies, it was stated that the methanol extract of mahogany seeds has good antioxidant properties, and the phenolic compounds contained in mahogany seeds can be the main contributors to antioxidant activity.Objective: This study aims to analyze the total phenolic content, measure the inhibition concentration 50% (IC50) and determine the antioxidant activity of mahogany (Swietenia mahagoni) seed infusion.Method: This type of research is experimental which begins with extraction using the infusion method at a temperature of 900C for 15 minutes followed by measuring the total phenolic content by reacting the infusion preparation and the Gallic acid respectively with the Follin-ciocalteu reagent. The antioxidant activity test was carried out using the DPPH (,-diphenyl--picrylhydrazyl) free radical scavenging method. Each absorbance was measured using UV-Vis Spectrophotometry. The phenolic test was measured by making a linear regression between concentration and absorbance, followed by calculating the total phenolic content. Meanwhile, the absorbance obtained for the antioxidant test was calculated by calculating the percent inhibition. The IC50 is determined by calculating the linear regression of the relationship between concentration and percent inhibition.Result: The results of the phenolic content were 2.531 ± 0.029 mg GAE/g, the IC50 value for gallic acid was 5.54 ppm, and for mahogany seed extract, the IC50 value was 47.04 ppm, which is a very strong antioxidant.Conclusion: This study concluded that the phenolic content of mahogany seed infusion extract contained phenolic compounds equivalent to 2.531 ± 0,029 mg GAE/g of gallic acid and that mahogany seed extract had very strong antioxidant activity.
Analysis Of Caffeine In Packaged Drinks Using Thin Layer Chromatography (TLC) and Spectrophotometry Method Muhammad Abdurrahman Munir; Emelda Emelda; Annisa Fatmawati; Nurhidayanti Nurhidayanti
Stannum : Jurnal Sains dan Terapan Kimia Vol 6 No 1 (2024): April 2024
Publisher : Department of Chemistry - Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jstk.v6i1.4564

Abstract

Caffeine is a natural alkaloid compound found in plants in the leaves, seeds and fruit. The decision of the Food and Drug Supervisory Agency regarding provisions for supervision of supplements in Chapter VIII Article 18 paragraph two states that caffeine levels must not exceed 150 mg/day. The aim of this research was to find out caffeine level in energy beverages. This research method applied the UV-Vis spectrophotometry method and the TLC method. The maximum wavelength obtained in this study was 272.55 nm and the linear results and correlation coefficient obtained were obtained. The linearity value obtained at y= 0.0381x + 0.3285 with the correlation equation R2= 0.9389. Caffeine levels obtained from “brand A” energy drink powder samples for first replicate at 49.59 mg/g, second replicate was 48.78 mg/g, third replicate was 47.15 mg/g, and “brand B” sample for first replicate was 31.5625 mg/g, second replicate at 32.375 mg/g. The caffeine levels obtained in the “brand C” sample from first replicate at 59.43 mg/g, second replicate was 60.28 mg/g, and third replicate was 60.09 mg/g. The results of the standard Rf value for caffeine standard were 0.7, the “brand A” energy drink powder sample was 0.75, the “brand B” sample was 0.75 and the “brand C” was 0.75. The conclusion from this research was that the maximum wavelength was 272.55 nm, which was slightly different from the theoretical maximum wavelength of pure caffeine, namely 273 nm. The caffeine levels obtained for samples of energy drink powder from brands A and B met the requirements because it was <50 mg/package and brand C did not meet the requirements because it was >50mg/package. The Rf value results are satisfactory because they were at range 0.2-0.8.
Co-Authors Aldilla Sari Utami Alfiana Deviana Allefeti Sandrika Annisa Fatmawati Audia N Rumbaremata Azizah, Abibatul Badri, Khairiah Haji Badrul, Ahlam Inayatullah Badrul, Hamid Alkhair Cahya Retna Ningtyas Chairunisa Ta Marsaoly Emelda Emelda Emelda Emelda emelda, emelda Emelda, Emelda Fera Erika Febranti Hamid Alkhair Badrul Ika Amalia Putri Inayatullah, Ahlam Ira Gusti Riani Joni Yoga Pratama Khairiah Haji Badri Kusumawardani, Nurul Lee Yook Heng Lee Yook Heng, Lee Yook Nailus Syafa’ah Nia Boru Ritonga Nunung Ismawati Nur Hidayati Nur Syafitri Nurhidayanti Nurhidayanti Pothitirat, Werayut Pratiwi, Sindi Rachmad bagas yahya supriyono Ristia Laura Amanda Siti Miftahus Sa’adah Veriani Aprilia Veriani Aprilia Yahya Supriyono Yuanita Sholihah