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All Journal E-Jurnal Medika Udayana Biotropika Biology, Medicine, & Natural Product Chemistry Jurnal Penelitian Pendidikan IPA (JPPIPA) Meditory : The Journal of Medical Laboratory Warmadewa Minesterium Medical Journal Community Services Journal (CSJ) Journal of Clinical Microbiology and Infectious Diseases
Indraningrat, Anak Agung Gede
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences

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ANTIBACTERIAL ACTIVITY SCREENING OF BACTERIAL ISOLATES ASSOCIATED WITH SEAWEEDS Caulerpa lentillifera AR, Sunthio Barran Cia; Indraningrat, Anak Agung Gede; Widhidewi, Ni Wayan; Wijaya, Made Dharmesti; Antara, Kadek Lila; Choe, Daegeun
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.4051

Abstract

Background: Caulerpa lentillifera is a species of green algae that has been cultivated widely in Indonesia for its nutritional contents and health benefit. This seaweed builds a strong association with bacterial community which play an important role in synthesizing various secondary metabolites including antibacterial activity. Aims:The research aim to explore the symbiotic relationship between C. lentillifera and its associated bacterial community, focusing on the potential of these bacteria to produce antibacterial metabolites.Methods:Bacterial cultivation was performed on three different media namely nutrient agar, plate count agar, and zobell marine agar. The obtained bacterial isolates were identified morphologically and subsequently were screened for their antibacterial activity based on perpendicular cross streak method against Staphylococcus aureus ATCC 25923, Streptococcus mutans FNCC 0405, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 700603. Results: 20 out of 28 bacterial isolates inhibited against at least one of the bacterial targets. Bacterial isolate encoded as NAR9 showed as the most promising bacterial isolates with length inhibition of 19 mm against S. mutans FNCC 0405. Amplification of 16S rRNA gene fragment of the NAR9 bacterial isolate followed by Sanger sequencing showed that the isolate NAR9 has been phylogenetically related to Marinobacter hydrocarbonoclasticus. Conclusion: Overall, this research provides a preliminary result of the untapped potential of bacteria associated with C. lentillifera as the source of antibacterial activity. 
BACILLUS SP RM3 ISOLATED FROM MANGROVE FOREST HAS POTENTIAL AS ANTIBACTERIAL ACTIVITY AND CYTOTOXICITY Maheswari, Ida Ayu Kartika; Indraningrat, Anak Agung Gede; Paramasatiari, Anak Agung Ayu Lila; Choe, Daegeun
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.4073

Abstract

Background: Antibacterial resistance is a critical health concern, driving the search for new compounds from natural sources such as mangrove-associated bacteria, which produce diverse secondary metabolites.Objective:  This research focused on studying the bioactivity of the extract of Bacillus sp. RM3, previously isolated from mangrove forests through an extraction method using ethyl acetate solvent.Methods: The Bacillus sp. RM3 isolate was fermented in ISP-2 media and extracted with ethyl acetate. The resulting crude extract was tested for antibacterial activity using the Kirby-Bauer method against Staphylococcus aureus ATCC 25923, Streptococcus mutans FNCC 0405 (Gram-positive), Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 700603 (Gram-negative). Cytotoxic effects were assessed via the Brine Shrimp Lethality Assay (BSLT).Results: The ethyl acetate extract displayed moderate antibacterial activity, evidenced by inhibition zone diameters between 5 to 10 mm. Triplicate measurements revealed the following average inhibition zone diameters: E. coli (9.65±1.62 mm), K. pneumoniae (7.88±1.92 mm), S. aureus (7.77±1.26 mm), and S. mutans (7.03±0.99 mm). Furthermore, the BSLT assay revealed an LC50 value of 504.586 ppm, which indicates the extract was toxic (LC50 1000 ppm).Conclusions: Overall, these results indicate the potential of Bacillus sp. RM3 isolate as a producer of antibacterial and cytotoxic compounds 
Antibacterial Activity of Pseudomonas Aeruginosa ISP1RL3 Against Multidrug Resistance Bacteria Indraningrat, Anak Agung Gede; Purnami, Pande Putu Christine Putri; Aryastuti, Anak Agung Sri Agung; Wijaya, Made Dharmesti
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.5643

Abstract

Seaweed-associated bacteria have a pivotal role to synthesize arrays of secondary metabolites. This study described a bacterial isolate encoded as ISP1RL3 that was isolated from seaweed Eucheuma cottonii. Ethyl acetate extracts of ISP1RL3 was screened against non-multidrug bacteria (Staphylococcus aureus ATCC 25923, Streptococcus mutans FNCC 0405, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603) and multi-drug resistance bacteria (Methicilin-resistance S. aureus (MRSA), E. coli ESBL, K. pneumoniae ESBL, dan A.baumanii ESBL). Our result showed that ISP1RL3 displayed rod structure, Gram negative and identified as Pseudomonas aeruginosa. The crude extract displayed strong antibacterial activity against all bacterial test with the range zone of inhibition of 10 mm – 18 mm. The GC-MS analysis detected the presence of 13 antibacterial compounds with four dominant moleculer were o-Xylene, Ethylbenzene, p-Xylene and Benzene, 1,3-dimethyl. Overall, this finding highlights the potency of seaweed-associated bacteria to synthesize active compounds against multidrug resistance bacteria.
Antibacterial Activity Screening Of Bacterial Isolates Associated With Seaweed Eucheuma cottonii From Coastal Area In Buleleng, Bali Purnami, Pande Putu Christine Putri; Indraningrat, Anak Agung Gede; Darmayasa, Ida Bagus Gede
Biotropika: Journal of Tropical Biology Vol. 10 No. 2 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.02.07

Abstract

Seaweeds like other marine organisms develop a strong association with prokaryotic communities, especially bacteria. Many of these associated bacteria play an important role in synthesizing secondary metabolites which are beneficial for their host, especially against predation or infection. In Indonesia, Eucheuma cottonii is one of the seaweed species that has been cultivated widely.  However, only limited study has been reported on bacterial isolates and their antibacterial activity from E. cottonii.  Bacterial isolates from E. cottonii were cultivated in six agar media namely Zobell Marine Agar, Nutrient Agar, Plate Count Agar, ISP-1, ISP-2, and Starch-M protein agar. Cultivation of bacterial isolates yielded 23 isolates with nine bacterial isolates were classified as Gram-positive bacteria and 14 isolates were grouped among Gram-negative bacteria. All the bacterial isolates were screened for their antibacterial activity against six bacterial indicator strains namely Staphylococcus aureus ATCC 25923, Streptococcus mutans FNCC 0405, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 700603 using perpendicular streak and agar block method. Six out of 23 bacterial isolates displayed antibacterial activity against at least one of the bacterial indicator strains. The bacterial isolate ISP1RL4 showed the highest antibacterial activity with average inhibition of  > 20 mm against all bacterial indicators. Overall, our result indicated the potential of bacterial isolates associated with E. cottonii as an antibacterial producer.
Co-Authors Adhiputra, I Ketut Agus Indra Anak Agung Sri Agung Aryastuti AR, Sunthio Barran Cia Arta, Ni Kadek Dewi Asri Tiara Choe, Daegeun Fridayanthi, Putu Utari Ida Ayu Agung Idawati Ida Bagus Gede Darmayasa Kadek Karang Agustina Kadek Lila Antara Kirtanayasa, I Gede Yoga Ayuning Made Dharmesti Wijaya Maheswari, Ida Ayu Kartika Natania, Evellyne Ni Nyoman Sri Budayanti Paramasatiari, Anak Agung Ayu Lila Prihantara, Komang Gede Yudha Purnami, Pande Putu Christine Putri Putri Herawati, Putri Putu Arya Suryanditha Setiabudy, Marta Udiyani, Desak Putu Citra Widhidewi, Ni Wayan Widowati, I Gusti Ayu Rai - Yanti, Ni Komang Semara