Ratna Sholatia Harahap
Department Of Animal Husbandry, Faculty Of Animal Science, Universitas Jambi.

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Journal : Buletin Peternakan

Association of Cytochrome P450 2A6 (CYP2A6) Gene Polymorphisms with Fatty Acid Traits in Indonesian Native Sheep Gilang Kurnia Abdillah; Kasita Listyarini; Ratna Sholatia Harahap; Asep Gunawan; Cece Sumantri
Buletin Peternakan Vol 45, No 3 (2021): BULETIN PETERNAKAN VOL. 45 (3) AUGUST 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i3.65604

Abstract

A member of the cytochrome P450 superfamily enzyme that play a role in microsomal fatty acids oxidation process encoded by cytochrome P450 2A6 (CYP2A6). This study aimed to examined the CYP2A6 gene polymorphisms and their association on fatty acid traits in Indonesian native sheep. A total of 299 rams aged 10-12 months with body weights between 20-30 kg used to identify the CYP2A6 gene polymorphism are consisted of 36 samples of barbados cross sheep (BCS), 35 samples of compass agrinak sheep (CAS), 20 samples of javanese fat tailed sheep (JFT), 36 javanese thin tailed sheep (JTT), 20 samples of garut sheep (GS), 45 samples of garut composite sheep (GCS), meanwhile for the fatty acid analysis was carried out by using 107 of loin samples from the total sheep. Identification of the CYP2A6 gene polymorphisms were performed using PCR-RFLP (Polymorphism Chain Reaction-Restriction Fragment Length Polymorphism) with the BSmAI restriction enzymes. The amplification product was 286 bp. Polymorphism were found in JFT, JTT, GCS and JS with GG and GT genotypes, while BCS, CAS, and GS were monomorphic with TT genotype. The CYP2A6 BSmAI polymorphism was in Hardy-Weinberg Equilibrium for JFT, JTT and JS, while BCS, CAS, GS, GCS, and combined was deviated based on chi square. A SNP g.49170107 G>T of the CYP2A6 gene polymorphism was significantly associated (P <0.05) with only erucic acid (C22:1n9). The GT genotypes had a higher value than GG genotypes The CYP2A6 gene could be used as a selection marker to improve fatty acid traits in Indonesian native sheep.
Polymorphisms KIF12 GENE|BfaI and its Association on Carcass Characteristics and Lamb Meat Quality Raudah Putri Ekawati; Ratna Sholatia Harahap; Kasita Listyarini; Cece Sumantri; Asep Gunawan
Buletin Peternakan Vol 46, No 4 (2022): BULETIN PETERNAKAN VOL. 46 (4) NOVEMBER 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i4.77029

Abstract

One of gene which involves on mediation an antioxidant cascade in beta cells as an intracellular target of excess fat intake is Kinesin Family 12 (KIF12). This study aims to identify the diversity of SNP g. 9617965 C>T KIF12 and association with carcass characteristic and meat quality. The total sample used were 102 ram DNA samples consisted of 10 barbados cross sheep (BCS), 52 javanese thin tail (JTT), 15 jonggol sheep (JS), 15 garut sheep (GS), and 10 compass agrinak sheep (CAS). Identification of the diversity of KIF12|BfaI was conducted using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. The results showed that the amplification product was 696 bp. The diversity of the KIF12 gene was found three genotypes namely CC, CT, and TT. The allele frequency in all types of sheep was in Hardy-Weinberg equilibrium. A SNP of the KIF12 was associated (P<0.05) with characteristic of carcass, including length of carcass. The association of KIF12 with meat quality was significant (P<0.05) too associated with pH and water holding capasity (WHC). It could be concluded that KIF12 gene is a potential candidate gene for carcass characteristic and meat quality traits in sheep
Association and Polymorphism of the APOA5 Gene Related to Carcass Characteristics and Lamb Quality in Sheep Novita Nur Anggraeni; Kasita Listyarini; Ratna Sholatia Harahap; Cece Sumantri; Asep Gunawan
Buletin Peternakan Vol 47, No 2 (2023): BULETIN PETERNAKAN VOL. 47 (2) MAY 2023
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v47i2.81956

Abstract

The apolipoprotein A-V (APOA5) gene is a candidate gene that plays an important role in carcass characteristics and lamb quality. This study aimed to association and polymorphism of the APOA5 gene with carcass characteristics and lamb quality in sheep. The breed of sheep were used 100 sheep consisting of Thin Tailed Sheep (TTS) (n=55), Compass Agrinac Sheep (CAS) (n=10), Garut Sheep (GS) (n=18), Garut Composite Sheep (GCS) (n=8), and Barbados cross sheep (BCS) (n=9). Identification of APOA5 gene polymorphism was performed using PCR-RFLP method and association study was performed using GLM analysis. The results showed that the APOA5 (g.26929941 C>T) gene polymorphism was polymorphic in TTS, CAS, GS, and GCS, while BCS was monomorphic. The APOA5 gene has three genotypes (CC, CT, and TT). Association showed that the APOA5 was a significantly (P<0.05) associated with carcass percentage and carcass length. The polymorphism of the APOA5 (g.26929941 C>T) was also significant (P<0.05) associated with lamb quality of the pH dan tenderness. The TT genotype showed greater carcass length and pH than the CC and CT genotypes. The CT genotype showed a greater percentage of carcass and tenderness than the CC and TT genotypes. The APOA5 gene helps improve sheep carcass characteristics and lamb quality.
Sex-Based Correlation and Regression between Body Weight and Morphometric Traits of Jawarandu Goats Harahap, Ratna Sholatia; Daulay, Winni Liani; Wibowo, Sarwo Edy; Gushairiyanto, Gushairiyanto; Wiyanto, Eko; Rosadi, Bayu; Ediyanto, Helmi; Depison, Depison
Buletin Peternakan Vol 50, No 1 (2026): BULETIN PETERNAKAN VOL. 50 (1) February 2026
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v50i1.114440

Abstract

The aims of this study was to explore the relationship between body weight and morphometric characteristics in Jawarandu goats raised in Tanjung Jabung Barat, Provincy Jambi. A total of 40 Jawarandu goats (24 males and 16 females) aged 12-24 months were utilized in this study. The traits recorded in this study included body weight and morphometric characteristic. These morphometric variables were later utilized in correlation, regression, and principal component analysis (PCA) to characterize the multivariate structure of body conformation in both male and female Jawarandu goats. Analysis statistics for all morphometric traits were calculated using IBM SPSS Statistics, Minitab, and RStudio. The result showed that the male Jawarandu goats exhibited superior morphometric performance (P<0.05) compared to females in several parameters, including horn length, muzzle circumference, head length, chest depth, rump height, leg circumference, and tail length. The PCA analysis results showed that the first two principal components were able to explain 61.6% of the total variation in the data, with Dimension 1 (51.4%) as the dominant component, showing a clear gap between male and female individuals. The correlation between body weight and body length in males was very strongly correlated (r = 0.817), whereas in females, the correlation was moderate (r = 0.550). The regression models varied among the combined population, males, and females, indicating sex-based differences in growth patterns and body proportions. This approach is particularly valuable in breeding and livestock management programs, especially in field conditions where direct weighing is often impractical.