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All Journal FIAT JUSTISIA: Jurnal Ilmu Hukum Jurnal El-Riyasah : Jurnal Kepemimpinan dan Administrasi Al-Ijtima`i: International Journal of Government and Social Science Jurnal Ilmiah Mahasiswa Ekonomi Pembangunan Gender Equality: Internasional Journal of Child and Gender Studies Cyberspace: Jurnal Pendidikan Teknologi Informasi Journal of Public Administration and Local Governance El-Usrah: Jurnal Hukum Keluarga Jurnal Trias Politika Jurnal Geuthee Jurnal Ilmiah Ilmu Administrasi Tax Literacy and Leadership Agility: Indigenous Peoples Awareness in Paying Taxes BISNIS & BIROKRASI: Jurnal Ilmu Administrasi dan Organisasi Kenanga : Journal of Biological sciences and Applied Biology jurnal administrasi politik dan sosial Journal of Governance and Social Policy
Muazzinah Muazzinah
Universitas Islam Negeri Ar Raniry, Aceh, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Social Sciences Other

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Formulasi Minuman Probiotik Kombinasi Sari Buah Mangga (Mangifera indica L.) dan Nanas (Ananas comosus L.) dengan Penambahan Susu Skim dan Bakteri Asam Laktat Fadliah, Lia; Kasrawati, Kasrawati; Riska Vatica, Jeny; Muazzinah, Muazzinah; Azzuhra, Nurul
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 6 No. 1 (2026): April 2026 (Article In Press)
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v6i1.9799

Abstract

Digestive tract disorders such as diarrhea, gastritis, constipation, and Irritable Bowel Syndrome (IBS) are common health problems among the Indonesian population. Probiotics are known to play an important role in maintaining the balance of intestinal microbiota, inhibiting the growth of pathogenic bacteria, and enhancing immune responses. Mango (Mangifera indica L.) and pineapple (Ananas comosus L.) contain vitamins, dietary fiber, antioxidants, and natural prebiotic compounds that support the growth of lactic acid bacteria (LAB). The addition of skim milk serves as an additional nutrient source for LAB to optimize the fermentation process. This study aimed to formulate a probiotic beverage based on a combination of mango and pineapple juices with the addition of skim milk and lactic acid bacteria, as well as to evaluate the total LAB count, lactic acid content, and vitamin C content. This research employed a laboratory experimental method using a post-test only group design. The treatments consisted of skim milk concentrations of 1%, 2%, and 3%, with the addition of 10% LAB starter culture. The evaluation included LAB enumeration using the Total Plate Count method, determination of lactic acid content by titration with 0.1 N NaOH, and vitamin C analysis using the iodimetric titration method. The results showed that the formulation containing 1% skim milk produced the highest LAB count, reaching 1.8 × 10¹⁰ CFU/mL. The lactic acid content ranged from 0.45% to 2.07%, with formulations containing 2% and 3% skim milk meeting the Indonesian National Standard (BSN) requirements (0.5–2.0%). The average vitamin C content was 0,056%, which was considerably higher than the standard range specified by SNI (0.01–0.1%). It can be concluded that the combination of mango and pineapple juices with skim milk has the potential to be formulated as a probiotic beverage that meets quality standards. Formulations containing 2% and 3% skim milk exhibited the best physicochemical characteristics, while the 1% skim milk formulation resulted in the highest LAB count. Keywords: probiotic beverage; mango juice; pineapple; skim milk; vitamin C; lactic acid bacteria.
Co-Authors Ade, Amuji Akhiar, Akhiar Ayubi, Fathurahman Al Azzuhra, Nurul Bantasyam, Saifuddin Bestari, Putri Cut Zamharira Fadhil, Amrul Fadliah, Lia Fathurrahman, Reza Fredinan Yulianda Kasrawati, Kasrawati M. Shabri Abd. Majid Maisura, Mira Maqfirah, Syarifah Rizqa Maqfrah, Tia Muhammad Sabaruddin, Muhammad Muslim Zainuddin Nashriyah Nashriyah Nawar, Makhisan Nur Aidar Putri Bintusy Syathi, Putri Bintusy Putri Marzaniar Putri Ramadhani, Putri Rahmah, Rauzatul Rahmatillah, Putri Rizki Ratnawati Ratnawati Riska Vatica, Jeny Saiful Akmal Sumardi Efendi Ulfa, Widya Winny Dian Safitri Wulandari, Dilya - Zahlul Pasha Zakki Fuad Khalil