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All Journal ODONTO Dental Journal Jurnal Kesehatan Gigi Padjadjaran Journal of Dentistry Journal of Indonesian Dental Association Jurnal Kreativitas PKM JITEKGI Jurnal Ilmiah dan Teknologi Kedokteran Gigi Interdental Jurnal Kedokteran Gigi (IJKG) Denta: Jurnal Kedokteran Gigi Jurnal Kedokteran Gigi Terpadu Jurnal Abdimas Kesehatan Terpadu Odonto dental journal
Elline, Elline
Departemen Konservasi Gigi, Fakultas Kedokteran Gigi, Universitas Trisakti, Jakarta
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Synthesis and Physical Characterization of Nano-Hydroxyapatite-Collagen-Epigallocatechin-3-Gallate Hydrogel Composite Amanda, Hiroko Gabriela; Elline, Elline; Fibryanto, Eko
Journal of Indonesian Dental Association Vol 5 No 1 (2022): April
Publisher : Indonesian Dental Association

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Abstract

Introduction: Most commonly used vital pulp therapy material is calcium hydroxide (Ca(OH)2) but it has several disadvantages. Previous studies found that nano-hydroxyapatite might induce reparative dentin with no tunnel defect and adding collagen can improve hydroxyapatite mechanical properties. The collagen can also increase pulp cell proliferation and differentiation. The addition of Epigallocatechin-3-Gallate (EGCG) to collagen gel can be beneficial in reducing pulp inflammation. Objective: The purpose of this study is to synthesize and analyze the physical characteristics of the nano-Hydroxyapatite-collagen-Epigallocatechin-3-Gallate hydrogel composite. Methods: Nano-hydroxyapatite from chicken egg shells, 0,2 g/mL collagen type I, and 10 mmol/L EGCG each dissolved in 2 mL deionized water with various ratio of nano-hydroxyapatite:collagen, 40:60 (group 1), 50:50 (group 2), and 60:40 (group 3). Each solution was mixed together then 2% HPMC was added into the mixture. In present study, we observed hydrogel composite pH value, gelation time, and injectability analysis. Results: The pH value in 1 hour stirring for each group was 8,05 ± 0,05, 8,43 ± 0,02, and 8,71 ± 0,04. All samples had 1 hour gelation time. Injectability for each group was 90,67%, 91,93%, and 95,03%. Conclusions: The nanohydroxyapatite, collagen and EGCG hydrogel composite has a potential physical characterization to be used as a vital pulp therapy material based on its pH value and injectable ability but further study should be considered in determining ideal gelation time. ABSTRAK Pendahuluan: Bahan perawatan pulpa vital yang paling umum digunakan adalah kalsium hidroksida (Ca(OH)2), tetapi bahan tersebut memiliki beberapa kekurangan. Penelitian sebelumnya menunjukkan nano-hidroksiapatit mampu merangsang pembentukan dentin reparatif tanpa tunnel defect dan penambahan kolagen mampu meningkatkan sifat mekanik hidroksiapatit. Penambahan Epigallocatechin-3-Gallate (ECGC) pada gel kolagen bermanfaat dalam mengurangi respon inflamasi pulpa. Tujuan: Penelitian ini dilakukan untuk mensintesis dan melakukan uji karakteristik fisik komposit hidrogel nano-hidroksiapatit-kolagen-Epigallocatechin-3-Gallate. Metode: Nano-hidroksiapatit dari cangkang telur ayam dilarutkan dengan 0,2 g/mL kolagen tipe I, dan 10 mmol/L EGCG masing-masing ke dalam air deionisasi dengan perbandingan rasio nano-hidroksiapatit dan kolagen, 40:60 (kelompok 1), 50:50 (kelompok 2), dan 60:40 (kelompok 3). Seluruh larutan dicampurkan dan ditambahkan HPMC 2%, lalu dilakukan uji karakteristik gel berupa uji pH, waktu gelasi, dan uji injektabilitas. Hasil: Nilai pH 1 jam setelah pengadukan secara berurutan adalah 8,05 ± 0,05, 8,43 ± 0,02, dan 8,71 ± 0,04 dengan waktu gelasi selama 1 jam. Injektabilitas secara berurutan adalah 90,67%, 91,93%, dan 95,03%. Kesimpulan: Bahan komposit hidrogel nano-hidroksiapatit, kolagen, dan EGCG memiliki karakteristik fisik yang berpotensi untuk digunakan sebagai bahan terapi pulpa vital jika dilihat dari nilai pH dan kemampuan injektabilitas yang diperoleh.
Fracture Toughness of Monolithic Zirconia and Lithium Disilicate CAD/CAM Endocrown Suci, Aldrina Wulan Eka; Ratnasari, Dina; Dwisaptarini, Ade Prijanti; Elline, Elline
Journal of Indonesian Dental Association Vol 6 No 2 (2023): October
Publisher : Indonesian Dental Association

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Abstract

Introduction: Endocrown is an indirect restoration following the endodontic treatment made of ceramic material as a substitute for post core crown. The endocrown is cemented to the inner wall of the pulp chamber and to the cavity margins to increase the macromechanical retention. Material commonly used are monolithic zirconia and lithium disilicate; both can be obtained using the CAD/CAM technique. Good endocrown restoration must be able to handle the chewing workload especially on the posterior tooth. Objective: To determine the difference in fracture toughness of monolithic zirconia and lithium disilicate CAD/CAM endocrown. Methods: This study used 6 endocrown CAD/CAM samples, attached to typodont prototype in 3D resin printing model produced by the printing machine. Samples were divided into 2 groups. Group A were monolithic zirconia endocrowns and group B were lithium disilicate endocrowns. The fracture toughness was calculated using the Universal Testing Machine (UTM). Results: Fracture toughness of the monolithic zirconia endocrown were 2.747 N and lithium disilicate were 769 N. The unpaired t test showed that there was significant difference in fracture toughness between groups (p < 0,05). Conclusion: Monolithic zirconia endocrown has a higher fracture toughness compared to the lithium disilicate endocrown, but lithium disilicate is more recommended as an endocrown restoration material because its fracture toughness is closer to masticatory load of posterior teeth.
Diametral Tensile Strength of Microhybrid and Nanohybrid Composite Resins Sundiharja Putri, Jesslyn Nathania; Elline, Elline
Journal of Indonesian Dental Association Vol 4 No 1 (2021): April
Publisher : Indonesian Dental Association

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Abstract

Introduction: Microhybrid and nanohybrid composite resins are commonly used due to their high diametral tensile strength, which indicates the resistance of a material to chewing in posterior tooth restoration. Both composite resins have been widely produced via various modifications of their composition. Objectives: To evaluate the diametral tensile strength of composite resins with microhybrid and nanohybrid fillers. Methods: In this experimental laboratory study, microhybrid (DenFilTM) and nanohybrid (DenFilTM N) composite resins were shaped into 10 specimens each in cylindrical molds (6 mm diameter × 3 mm height) by the bulk-fill technique, and the upper layer was flattened using mylar strips and then polymerized using a light-curing unit for 20 s. Then, composite resin samples were immersed in cell culture plates filled with 2.5 mL of artificial saliva in a 37°C incubator for 24 h. Dimensions of the soaked specimens were examined using a digital caliper and tested using a universal testing machine. Results: The diametral tensile strength values for microhybrid and nanohybrid composite resins were 41.67 MPa and 45.42 MPa, respectively. Conclusion: There was no significant difference in the diametral tensile strength of microhybrid and nanohybrid.
Antibacterial and antibiofilm effect of moringa oleifera leaves on bacteria associated with endodontic-periodontal lesions: an experimental study Darma, Joshua; Amin, Meiny Faudah; Elline, Elline; Komariah, Komariah; Kurnia, Dikdik
Padjadjaran Journal of Dentistry Vol 37, No 3 (2025): November 2025
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol37no3.59353

Abstract

Introduction: Sodium hypochlorite (NaOCl) is the gold standard irrigation solution in root canal treatment; however, its clinical use is limited by its potential toxicity to periodontal tissue. Moringa oleifera is a natural plant known for its antibacterial properties. The study aims to analyze the potential antibacterial and antibiofilm effects of M. oleifera leaves on bacteria associated with endodontic-periodontal lesions. Methods: An experimental study was conducted using samples of P. gingivalis ATCC 33277 and S. sanguinis ATCC 10556 cultured on BHI agar before exposure to M. oleifera leaf extract at concentrations of 80, 60, 40, and 20%. A 5.25% NaOCl solution served as the positive control, while distilled water was used as the negative control. The minimum inhibitory concentration (MIC) was determined using the tube dilution method with BHI medium and defined as the lowest concentration that inhibited bacterial growth. The minimum bacterial concentration (MBC) was determined by subculturing inoculum from tubes that showed no visible growth in the MIC test. A standard plate count method was used to measure the growth density of P. gingivalis and S. sanguinis. The antibiofilm assay evaluated biofilm attachment using crystal violet staining. Biofil density was quantified using spectrophotometry at 570nmto measure the amount of transmitted light. A One-way ANOVA Test was applied to the normally distributed data, while the Kruskal-Wallis Test was used for non-normally distributed data. Post-hoc tests were performed to determine significant differences between groups, with a significance level set at p < 0.05. Result: The antibacterial test showed a significant effect of M. oleifera leaf extracts on the elimination of P. gingivalis (p = 0.002) and S. sanguinis (p = 0.001). The antibiofilm tests also showed a significant difference between among M. oleifera extract groups. Conclusion: M. oleifera leaf extract at concentrations of of 60 and 80 % exhibits antibacterial and antibiofil effects against P. gingivalis and S. sanguinis.
The Effect of Adding Patchouli Essential Oil (Pogostemon Cablin Benth.) on Setting Time Of GIC Prawira, William; Pratiwi, Deviyanti; Puspitawati, Ria; Elline, Elline; Binartha, Cipthadi Tri Oka
Jurnal Kesehatan Gigi Vol. 12 No. 1 (2025): December 2025
Publisher : Jurusan Kesehatan Gigi, Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jkg.v12i1.12391

Abstract

Essential oils are widely incorporated into dental materials to enhance their antibacterial properties, including patchouli essential oil (PEO) derived from Pogostemon cablin Benth. PEO contains bioactive compounds such as Patchouli Alcohol (PA), phenols, steroids, and terpenoids, which contribute to its antimicrobial activity. This study aimed to evaluate the effect of PEO addition on the setting time of glass ionomer cement (GIC). Twenty cylindrical GIC specimens (2 mm × 10 mm) were tested using Vicat’s needle and divided into four groups based on the powder:PEO:liquid ratios: Group A (control), Group B (2:1:1), Group C (3:1:2), and Group D (3:2:1). Data were analysed using One-Way ANOVA and Tukey’s test (p < 0.05). The addition of PEO to GIC’s liquid is significant (p < 0,05)  decreased the setting time of group B, C, and D. Modifying GIC with PEO showed a significant decrease in setting time in group B with a comparison ratio (2 : 1 : 1). All results remained within ISO 9917-1:2007 limits. Further studies are needed to assess long-term stability and mechanical performance.
Co-Authors Amanda, Hiroko Gabriela Anastasia Elsa Prahasti Anastasia Elsa Prahasti Andrian Nova Fitri Aryadi Aryadi Binartha, Cipthadi Tri Oka Clara Pricilla Suarthana Darma, Joshua Deviyanti Pratiwi Diana Puspa Indah Dikdik Kurnia Dwisaptarini, Ade Prijanti Ferry Sandra Fibryanto, Eko Harryanto Wijaya James Hartono Santoso Janti Sudiono Joko Kusnoto Juliawati, Mita Komariah Komariah Lambertus, Reynaldo Meiny Faudah Amin Prawira, William Ratnasari, Dina Ria Puspitawati Suci, Aldrina Wulan Eka Sundiharja Putri, Jesslyn Nathania Suwartini, Tien Teguh, Sharren Yohana Yusra