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All Journal LINGUA LITERA : journal of english linguistics and literature Jurnal Basicedu Jurnal Kreativitas PKM Jurnal Analis Medika Biosains (JAMBS) Narra J KESANS : International Journal of Health and Science Jurnal Basicedu JSN : Jurnal Sains Natural Bomba: Jurnal Pembangunan Daerah
Puspawati, Puspawati
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Journal : Jurnal Analis Medika Biosains (JAMBS)

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Malaria Density in Peripheral Blood Smear on Positive Cases of Malaria on Residences of Jaro South Kalimantan Nurmansyah, Dian; Khasanah, Maulisa; Wahyuni, Rizka Ayu; Puspawati, Puspawati
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.375

Abstract

Malaria, a severe infectious disease caused by Plasmodium parasites, remains a significant health challenge in endemic regions of Indonesia, particularly in areas with favorable environmental conditions for Anopheles mosquitoes. The liver is a critical organ involved in the early stages of malaria infection. This study investigates malaria density in confirmed malaria cases in Jaro District, South Kalimantan. The aims of this study was to determine the parasitemia density in individuals diagnosed with malaria in the Jaro District, contributing to the understanding of malaria severity in this region. A descriptive survey approach was employed to measure malaria density in 15 respondents with confirmed Plasmodium falciparum infections. Total sampling was used, and blood specimens were collected and analyzed in the clinical pathology laboratory of RS Badaruddin Kasim. Blood smears were stained using 3% Giemsa stain, and parasitemia density was determined microscopically following the Ministry of Health's 2012 guidelines. The study identified varying levels of parasitemia density among the respondents: 33.3% had a low parasitemia density (+), 20% had moderate densities (++ and +++), and 26.7% exhibited high density (++++). The highest proportion of cases showed a low parasitemia density. The analysis of malaria density in the Jaro District reveals significant variability in parasitemia levels among patients. This variability highlights the importance of continuous monitoring and tailored interventions to manage malaria effectively in endemic regions. Understanding parasitemia density is crucial for assessing disease severity and implementing effective treatment
Lymphocyte Cell Count Profile based on Widal Titer On Pediatric Typhoid Fever Nurmansyah, Dian; Fayumi, Syihab; Nisa, Shalehatun; Puspawati, Puspawati; Sasmitha, Maya; Mudzakkir, Musyirrah; Arsyad, Muhammad
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.480

Abstract

Typhoid fever remains a significant public health concern in Indonesia, which has the third-highest global case rate, with 350–810 cases per 100,000 people and a fatality rate of 3.1–10.4%, primarily due to delayed diagnosis and treatment. Children aged 5–14 years are most affected, with transmission occurring via contaminated food, water, or poor hygiene. Salmonella typhi invades intestinal immune tissues (Peyer’s patches), triggering responses from T and B lymphocytes, though bacterial endotoxins can suppress bone marrow function, altering leukocyte levels. This study analyzed the relationship between Widal titer and lymphocyte counts in pediatric typhoid patients using a cross-sectional design. Data from 526 patients at Graha Medika Martapura Clinic and Ratu Zalecha Hospital (January 2023–February 2025) were analyzed statistically, with inclusion criteria of Widal titer ≥1/320 and complete blood count results. Results showed 18.8% of patients had elevated lymphocytes, 79.3% had normal levels, and 1.9% exhibited lymphocytopenia. Higher Widal titers (1/640) correlated with lower lymphocyte counts (15.8% elevated vs. 23.0% at 1/320), suggesting immune suppression mediated by IL-6 through specific signaling pathways. Paradoxically, some cases showed lymphocytosis (18.8%), likely due to cytokine-induced immune modulation. Statistical analysis revealed a minimal correlation (R²=0.006), indicating Widal titer explains only 0.6% of lymphocyte variation. These findings demonstrate that while Widal titer has limited predictive value for lymphocyte changes, immune response mechanisms, particularly involving IL-6, play a critical role in typhoid fever's hematological manifestations.
Co-Authors Alpian, Yayan Anwar, Aang Solahudin Becker, Sören L. Buehler, Nina Dewi, Rooswidiawati Fayumi, Syihab Junus Widjaja Khasanah, Maulisa Kristi, Priska PP. Kusumasari, Peni Kusumasari, Rizqiani A. Lystirini, Eka Mudzakkir, Musyirah Mudzakkir, Musyirrah Muhammad Arsyad Murhandarwati, Elsa H. Nelfira Nisa, Shalehatun Norhayati Norhayati, Norhayati Nurul Amalia Putri Ayanti, Bio Rahmi Muthia Rakhmatullah, Aditya Noviadi Rizki P, Hayati Sasmitha, Maya Sasmitha, Maya Herliana Saufi, Muhammad Schneitler, Sophie Sholikhah, Eti N. Sy, Issa Syairaji, Muhammad Tanzil, Yurnia Tanzil, Yurniah Valentine Gunasari, Lala Foresta Wahyuni, Rizka Ayu Yessy Prima Putri