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All Journal Jurnal Biologi dan Pembelajarannya (JBP) Jurnal Biologi Tropis BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Best Journal (Biology Education, Sains and Technology) Jurnal Biogenerasi BioEksakta : Jurnal Ilmiah Biologi Unsoed Journal La Lifesci El-Mujtama: Jurnal Pengabdian Masyarakat Jurnal Pijar MIPA BIO-CONS: Jurnal Biologi dan Konservasi Jurnal Biologi
Nasution, Rizki Amelia
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Physics Public Health Social Sciences Veterinary Other

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Formulasi Sediaan Sheet Mask Ekstrak Daun Murbei (Morus alba L.) sebagai Anti Acne Terhadap Bakteri Propionibacterium acnes Harahap, Nurul Aina; Mayasari, Ulfayani; Nasution, Rizki Amelia
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 7 No 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i2.13000

Abstract

This study aimed to determine the antibacterial activity of mulberry leaf extract and formulate Sheet Masks containing the extract at different concentrations of 4%, 6%, 8%, and 10%. The method involved antibacterial activity testing using the disk diffusion method with positive control (clindamycin and Wardah Sheet Mask), negative control (DMSO), and three replications. Organoleptic and physical evaluations of the Sheet Mask formulation were also conducted. The results showed that mulberry leaf extract inhibited the growth of Propionibacterium acnes, with inhibition zones of 6.0 mm at 4%, 9.45 mm at 6%, 12.57 mm at 8%, and 13.95 mm at 10%. For the Sheet Mask formulations, the inhibition zones were 10.69 mm for the positive control (clindamycin), 0.1 mm for F0, 9.78 mm for F1 (8%), and 10.29 mm for F2 (10%). Organoleptic evaluation revealed that the most preferred formulations in terms of color, aroma, and texture were F0, F2, and F1, respectively. Physical evaluation confirmed that the Sheet Mask formulations met quality standards. In conclusion, mulberry leaf extract demonstrated antibacterial activity against Propionibacterium acnes, with the most effective concentrations being 8% and 10%. Keywords: antibacterial, acne, Sheet Mask, mulberry, Propionibacterium acnes
Potensi Formulasi Sediaan Hair Tonic Herbal Ekstrak Daun Sirih Hijau (Piper betle L.) Sebagai Antifungi Pityrosporum ovale dan Microsporum canis Secara In Vitro sundari, wiwik; Mayasari, Ulfayani; Nasution, Rizki Amelia
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 2 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.2.14986

Abstract

Pityriasis capitis (ketombe) adalah kelainan kulit kepala yang ditandai dengan sisik putih terasa gatal yang disebabkan oleh sejumlah jamur. Hair tonic yang beredar banyak berasal dari bahan sintetis seperti minoxidil, metil paraben dan lain sebagainya yang dapat menyebabkan iritasi kulit. Daun sirih (Piper betle L.) memiliki senyawa aktif seperti flavonoid, alkaloid, tanin, saponin, terpenoid dan steroid yang berperan sebagai antifungal yang dimodifikasi menjadi produk sediaan herbal hair tonic. Penelitian ini bertujuan untuk mengetahui kemampuan zona hambat ekstrak daun sirih (Piper betle L.) terhadap pertumbuhan Pityrosporum ovale dan Microsporum canis serta mengetahui kemampuan zona hambat sediaan hair tonic herbal ekstrak daun sirih (Piper betle L.) dalam pertumbuhan jamur Pityrosporum ovale dan Microsporum canis. Dalam penelitian ini digunakan 4 perlakuan konsentrasi ekstrak daun sirih (Piper batle L.) dan 3 ulangan menggunakan metode difusi cakram. Hasil yang diperoleh ekstrak daun sirih (Piper betle L.) memiliki efektivitas dalam menghambat pertumbuhan jamur Pityrosporum ovale dengan konsentrasi 15% (1.71 mm), 20% (8.88 mm), 25% (10.03 mm) dan 30% (11.28 mm). Pada jamur Microsporum canis ekstrak daun sirih (Piper betle L.) tidak memiliki efektivitas dalam menghambat pertumbuhan jamur. Sediaan hair tonic herbal ekstrak daun sirih (Piper betle L.) memiliki efektivitas dalam menghambat pertumbuhan jamur Pityrosporum ovale dengan konsentrasi 20% (1,15%), 25% (5,61) dan 30% (10,01). Kesimpulan penelitian ini menunjukkan sediaan herbal hair tonic ekstrak daun sirih (Piper betle L) dapat menghambat jamur Pityrosporum ovale Kata kunci : Microsporum canis, Pityrosporum ovale, Hair tonic ekstrak daun sirih
Kombinasi Ekstrak Daun Kemangi (Ocimum basilicum L.) dan Cangkang Telur sebagai Masker Gel Peel Off Anti Acne Lestari, Ayuni; Nasution, Rizki Amelia; Selvia, Irda Nila
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 2 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.2.15654

Abstract

Daun kemangi (Ocimum basilicum L.) telah lama dikenal sebagai tanaman herbal dengan aktivitas antibakteri dan antiinflamasi, sedangkan cangkang telur mengandung mineral dan protein yang dapat memperbaiki kesehatan kulit. Kombinasi kedua bahan alami ini berpotensi dikembangkan menjadi produk perawatan kulit, khususnya masker anti jerawat. Penelitian ini bertujuan untuk mengembangkan masker gel peel-off anti jerawat berbahan kombinasi ekstrak daun kemangi dan cangkang telur. Daun kemangi dipilih karena kandungan flavonoid, alkaloid, dan tanin yang bersifat antibakteri, sedangkan cangkang telur kaya akan kalsium dan protein yang bermanfaat untuk kesehatan kulit. Metode penelitian meliputi ekstraksi maserasi, uji fitokimia, dan uji aktivitas antibakteri terhadap Propionibacterium acnes menggunakan metode difusi cakram. Hasil uji fitokimia menunjukkan adanya senyawa aktif seperti flavonoid, alkaloid, dan tanin. Uji antibakteri menunjukkan bahwa formula dengan konsentrasi 3% ekstrak daun kemangi dan cangkang telur menghasilkan zona hambat sebesar 9,36 mm, sedangkan kontrol positif (klindamisin) menghasilkan 14,1 mm. Evaluasi karakteristik fisik masker gel (pH, daya lekat, daya sebar, waktu kering) memenuhi standar SNI. Kesimpulannya, kombinasi ekstrak daun kemangi dan cangkang telur berpotensi sebagai bahan aktif dalam masker gel peel-off anti jerawat, meskipun efektivitasnya masih di bawah antibiotik standar. Kata kunci: Ekstrak daun kemangi, cangkang telur, masker gel peel off, Propionibacterium acnes
Aktivitas Bakteri Asam Laktat (BAL) dan Kadar Antioksidan pada Yoghurt Ampas Tahu dengan Penambahan Sari Bunga Telang (Clitoria ternatea) Alfira, Della; Rasyidah, Rasyidah; Nasution, Rizki Amelia
BIO-CONS : Jurnal Biologi dan Konservasi Vol. 6 No. 2 (2024): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v6i2.2095

Abstract

  Yogurt dengan penambahan sari bunga telang digunakan sebagai pewarna dan dapat meningkatkan jumlah antioksidan dalam yogurt. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan sari bunga telang (Clitoria ternatea) terhadap kadar antioksidan dan aktivitas bakteri asam laktat yang terdapat pada yoghurt ampas tahu. Penelitian ini menggunakan metode eksperimental dengan menggunakan 4 perlakuan, yaitu P0 : tanpa penambahan sari bunga telang, P1: penambahan sari bunga telang sebanyak 6%, P2: penambahan sari bunga telang sebanyak 8%, P3: penambahan sari bunga telang sebanyak 10 %. Analisis data yang dilakukan yakni secara deskriptif. Hasil penelitian menunjukkan bahwa yoghurt dengan penambahan sari bunga telang pada kosentrasi 6% (P1), 8% (P2), 10% (P3) tidak berpengaruh terhadap aktivitas BAL. Pada antioksidan penambahan sari bunga telang terbukti meningkatkan aktivitas antioksidan dalam yoghurt, yaitu pada konsentrasi 10% (P3) dengan nilai  aktivitas antioksidan 100,67 ppm.
Isolasi dan Identifikasi Mikroba Penyebab Kontaminasi dari Udara di Laboratorium Kultur Jaringan Tumbuhan UIN-SU Medan Sucahyo, Ahmad Imam; Manalu, Kartika; Nasution, Rizki Amelia
Jurnal Biologi Vol. 1 No. 1 (2023): November
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/biology.v1i1.1931

Abstract

Plant tissue culture laboratory is a technique for growing cells, tissues or slices of plant organs which are often called explants in the laboratory in an artificial media containing aseptic (sterile) nutrients to become a whole plant. This research aims to know the isolation and identification of contaminating microbes in the plant tissue culture laboratory and to know the types of contaminating microbes in the plant tissue culture laboratory. This research was conducted from december 2022 to january 2023 at the Wahdatul Ulum plant tissue culture laboratory, uinsu medan with 5 main sampling points.the sampling point consists of an incubation room, planting room and practicum space which is divided into 3 collection points. Staining tests and biochemical tests are conducted at the USU faculty of mathematics and natural sciences microbiology laboratory. The results of the study found 23 bacterial isolates that contamined the wahdatul ulum plant tissue culture laboratory at UINSU Medan consisting of the genera bacillus, lactobacillus, streptobasil, aeromonas, pseudomonas, serratia, bacteroides and acinetobacter while the contaminating fungi were Mucor sp. Contamination occurs due to mobility and activity in the laboratory room.
Screening of Antimicrobial Producing Bacteria from Berawe Beach Sand on Kampai Pangkalan Susu Island against Pathogenic Bacteria Annisa, Rafika; Manalu, Kartika; Nasution, Rizki Amelia
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6334

Abstract

Sand is naturally a place to live, grow and develop some marine animals and microorganisms, usually bacteria originating from the sea area that have a large number of bioactive compounds that can produce various kinds of secondary metabolites for further production of antimicrobials. The purpose of this study was to obtain bacteria that have the potential to produce antimicrobials and to characterize bacterial isolates on the sand of Berawe Beach, Kampai Island, Pangkalan Susu. In this study several stages were carried out, namely sampling, isolation, purification, morphological characterization, antimicrobial activity test, gram staining, biochemical test and determining the bacterial genus. The results of this study obtained 9 isolates (SP14A. SP15A, SP16A, SP24A, SP24B, SP35A, SP44A, SP44B and SP45A) which have the potential as antimicrobial producers. Characterization of antimicrobial bacterial isolates from the sand of Berawe Beach, Kampai Island, Pangkalan Susu, namely isolate SP14A, which is a gram- positive bacterium in the form of streptobacilli. SP15A, SP24B and SP44B isolates were coccus-shaped gram-positive bacteria. SP16A, SP35A, SP44A and SP45A isolates were gram-positive bacteria in the form of bacilli. And isolate SP24A is a gram-negative bacterium in the form of streptobacilli. All isolates produce catalase enzymes but do not use carbon and energy. Isolates that are motile (SP14A, SP15A, SP16A, SP24A, SP35A, SP44A and SP45A) can ferment glucose while non-motile (SP24B and SP44B) cannot ferment glucose. These bacteria come from the genera Bacillus, Micrococcus, LactoBacillus and Alcaligenes. Species of Bacillus pumilus, Bacillus subtilis, Bacillus firmus, LactoBacillus bulgaricus, Micrococcus luteus and Alcaligenes eutrophus.
Ultilazation of Bakteria Pseudomonas aeruginosa and Bacillus Subtilos as Bioremediation of Metal Pb in Textile Waste Hasibuan, Ira Aqrabi Br; Manalu, Kartika; Nasution, Rizki Amelia
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6820

Abstract

The Indonesian textile industry is developing to meet people's needs for clothing. 3916 industries in Indonesia exist in 2021, but the number of these industries is not balanced with liquid waste processing. One alternative way to reduce heavy metal levels in waste is bioremediation. The aim of this research is to determine how incubation time and the number of Pseudomonas aeruginosa and Bacillus subtlis bacteria influence the reduction of Pb metal levels in textile waste. Several steps include the bioremediation process, colony counting, measuring Pb metal levels, measuring temperature levels, measuring pH, and data analysis. Bioremediation results: Pseudomonas aeruginosa and Bacillus subtilis bacteria succeeded in reducing Pb metal levels in textile waste with an incubation time of 14 days. The results show that this bacterium is able to reduce Pb metal levels in textile waste with the addition of a 1:1 concentration of 56%, a 1:2 concentration of 86%, and a 2:1 concentration of 92. These results show that this bacterium is successful as a bioremediation agent in reducing Pb metal levels in waste.
Population and Characterization of Rhyzospheric Bacteria of Pineapple Plant (Ananas comosus L. Merr) on The Highland Land of Lumban Sihite Village, Regency Dairi Ginting, Monika; Manalu, Kartika; Nasution, Rizki Amelia
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6822

Abstract

The rhizosphere is the part of the soil around the roots of plants which is known to have a greater number of rhizosphere bacteria compared to the soil far from the roots. This research aims to determine the population and characteristics of rhizosphere bacteria from pineapple plants (Ananas comosus L. Merr) on the highland land of Lumban Sihite village, Dairi district. The stages used in this research were taking samples at three different points, then carrying out multilevel dilutions, inoculating them into petri dishes filled with PCA (Plate Count Agar) media, then counting the growing colonies using the TPC (total plate count) method, purification, morphological characterization, gram staining and biochemical tests. The research results obtained at point 3 had the largest population of 463.96 x 10⁴ CFU/g, while point 1 had the lowest population, namely 20.07 x 10⁴ CFU/g. Based on the results of morphological, gram staining and biochemical characterization of bacterial isolates, the isolates were identified as belonging to the genus Azospirillum, Bacillus, Pseudomonas and Azotobacter.
Esting of Fungal Antagonists from The Rhyzosphere of Mother-in-Law's Tongue (Sansevieria trifasciata Prain) as a Fungal Control Agent Fusarium sp. Julianty, Dwita; Manalu, Kartika; Nasution, Rizki Amelia
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7522

Abstract

Fusarium sp. is a serious problem for various plant commodities because of its ability to act as a pathogen and cause damage to plant anatomy and morphology. The rhizosphere fungus of the mother-in-law's tongue plant has more abundant inhibitory mechanisms and is able to directly inhibit hyphae growth and suppress the growth of pathogenic fungi, so it was chosen as a biocontrol agent in this research. The aim of this research was to determine the antagonistic properties and ability of the rhizosphere fungus from the mother-in-law's tongue plant (Sansevieria trifasciata Prain) against the fungus Fusarium sp. This research was carried out with data analysis starting with sterilization and making media, then taking soil samples from the rhizosphere of the mother-in-law's tongue using the purposive random sampling to continue with the isolation and purification stage of the rhizosphere fungus of the mother-in-law's tongue plant. Antagonist test of fungi from the rhizosphere of snake plant against the fungus Fusarium sp. carried out using a double culture method (dual culture). The data obtained were then analyzed using Microsoft Excel 2013 to obtain an average inhibition which shows the potential of fungi from the rhizosphere of the plants as controlling agents for the fungus Fusarium sp. The results of isolation and antagonistic tests, the five isolates of snake plant rhizosphere fungus (Sansevieria trifasciata Prain) have varying inhibitions ranging from very weak to moderate. The highest inhibition against Fusarium sp. was shown by isolate JDT12, while the lowest inhibition was shown by isolate JDT11. The antagonism mechanism found by isolate JDT12 is the antibiosis mechanism.
Co-Authors Ainun, Nabiilah Hurul Alfira, Della Andini, Pinkhan Aliffia Sekar Aulia, Rahma Azhari, Raisa Diantiwi, Dinda Fatimah Azzahra Ginting, Monika Harahap, Nurul Aina Hasibuan, Ira Aqrabi Br Irda Nila Selvia Julianty, Dwita Khadijah, Nur Khairunnisa, Eliza Lestari, Ayuni Maisyaroh, Dinda Manalu, Kartika Mawaddah, Fitri Asyiatun Mayasari, Ulfayani Nabila, Vara Nurcahyani, Nadya Pasaribu, Nurul Alda Rafika Annisa, Rafika Raselia, Citra Rasyidah Rasyidah Sejati, Renny Novita Asri Simarmata, Faried Raja Siregar, Amira Falah Siregar, Khoirul Bahria Sucahyo, Ahmad Imam Syahputri, Sufi Amir Utami, Mutiara Alya Wiwik Sundari Zaini, Nurlayla