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Aktivitas Antibakteri Amoksisilin Terhadap Bakteri Gram Positif Dan Bakteri Gram Negatif Surah Maida; Kinanti Ayu Puji Lestari
Jurnal Pijar Mipa Vol. 14 No. 3 (2019): September
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Amoksisilin merupakan antibiotik yang sering digunakan dalam berbagai pengobatan dan efektif membunuh bakteri Gram positif dan Gram negatif. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri amoksisilin terhadap bakteri Gram positif (Bacillus subtilis, Staphylococcus aureus) dan bakteri Gram negatif (Escherichia coli, Pseudomonas aeruginosa). Metode penelitian ini meliputi pembuatan konsentrasi antibiotik (10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm); pengujian antibakteri dengan metode kertas cakram serta pengkuran zona hambat yang terbentuk. Didapatkan data bahwa rata-rata zona hambat yang terbentuk dari keempat bakteri tersebut sebesar 0,03 Ã¢â¬â 0,44 mm. Pada konsentrasi 30 ppm, menunjukkan bahwa bakteri Staphylococcus aureus dan Escherichia coli menghasilkan zona hambat yang terluas yaitu sebesar 0,38 mm dan 0,44 mm. Disimpulkan bahwa sensitivitas keempat bakteri uji bersifat intermediet terhadap antibiotik amoksisilin.ÃÂ ÃÂ

Antibacterial Activity of Beluntas (Pluchea indica L.) Leaves Extract using Different Extraction Methods Kinanti Ayu Puji Lestari; Putri Priliawati Pranoto; Sofiyah Sofiyah; Majidah Musyirah; Faizatin Isnaini Pratiwi
Jurnal Riset Biologi dan Aplikasinya Vol. 2 No. 2 (2020)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v2n2.p49-54

Escherichia coli and Bacillus subtilis are bacteria that cause disease in the digestive tract. Pluchea indica L. had the pharmacological activity of antiseptic power against bacteria that cause digestive tract infections because of contained antibacterial compounds. The study on the comparison of extraction methods will enable the public to choose a better extraction method to use as an antibacterial agent. This study aimed to identify the content of antibacterial compounds in Pluche indica L. and to determine the antibacterial ability of Pluche indica L. extract using maceration, percolation, and soxhletation methods. The results showed that Pluchea indica L. contains flavonoids, alkaloids, saponins, and tannins. The highest diameter inhibition of Escherichia coli and Bacillus subtilis was obtained from Pluchea indica L. leaves extract extracted by using the Soxhletation method.

EDUKASI PERTOLONGAN PERTAMA GAWAT DARURAT (PPGD) SEBAGAI PENGUAT KETERAMPILAN PADA SISWA SMK Silfiana Nisa Permatasari; Kinanti Ayu Puji Lestari
Jurnal Bakti untuk Negeri Vol 2 No 2 (2022): JBN
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.196 KB)

An emergency is an event that occurs suddenly, resulting in a person requiring immediate and appropriate treatment and assistance. Everyone needs first aid knowledge and skills. Emergency First Aid (PPGD) requires assistance and initial treatment that can be carried out by ordinary people, including students of SMK Pharmacy Kapasari Surabaya. The target of this activity is class XII SMK students consisting of 29 students. The purpose of organizing this educational activity is to strengthen skills so that participants receive provisions that can be applied to the community. This counseling will be held on May 18, 2022, located at Graha Ilmu, Jl. Intermediate Potato No. 81. Service activities use simulation and role play methods as well as discussions to increase knowledge about the material. Community service activities produce changes that are in accordance with the output targets, namely increasing students' knowledge, attitudes, and skills about First Aid with CPR (Cardiopulmonary Resuscitation) or Basic Life Support, fractures, burns, choking and electric shock.

Antibacterial Assay Of Cyperus rotundus Rhizomes Against Gram Positive And Gram Negative Bacteria: Uji Potensial Antibakteri Umbi Rumput Teki (Cyperus rotundus) terhadap bakteri Gram positif dan bakteri Gram negatif Diana Yuni Anjarsari; Kinanti Ayu Puji Lestari
Journal Pharmasci (Journal of Pharmacy and Science) Vol. 7 No. 2 (2022): Journal Pharmasci (Journal of Pharmacy and Science)
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v7i2.294

Studies on antibacterial tests derived from natural ingredients were more intensively carried out. This was to reduce the side effects of using antibiotics related to the incidence of pathogenic bacteria. Nut grass tubers were one of the natural ingredients that are widely known for their benefits for human health. Nut grass tubers were also known to contain antibacterial compounds such as flavonoids, tannins, saponins and so on. This study aims to determine the potential of nut grass tuber extract as antibacterial of Gram positive and Gram negative. This study used a completely randomized design with the treatment of teki grass tuber extract with concentrations of 25%, 50% and 100%. Based on the research, it was known that the nut grass tubers were able to inhibit the growth of Gram positive and Gram negative bacteria in the category of weak to moderate ability. This study used the treatment of teki grass tuber extract with concentrations of 25%, 50% and 100%. Based on the research, it was found that the tuber of teki grass was able to inhibit the growth of Gram positive bacteria with the highest inhibition at 100% concentration of 5.53 mm and Gram negative with the highest inhibition at 100% concentration of 6.57 mm.

EDUKASI PERTOLONGAN PERTAMA GAWAT DARURAT (PPGD) SEBAGAI PENGUAT KETERAMPILAN PADA SISWA SMK Silfiana Nisa Permatasari; Kinanti Ayu Puji Lestari
Jurnal Bakti untuk Negeri Vol 2 No 2 (2022): JBN
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jbn.v2i2.964

An emergency is an event that occurs suddenly, resulting in a person requiring immediate and appropriate treatment and assistance. Everyone needs first aid knowledge and skills. Emergency First Aid (PPGD) requires assistance and initial treatment that can be carried out by ordinary people, including students of SMK Pharmacy Kapasari Surabaya. The target of this activity is class XII SMK students consisting of 29 students. The purpose of organizing this educational activity is to strengthen skills so that participants receive provisions that can be applied to the community. This counseling will be held on May 18, 2022, located at Graha Ilmu, Jl. Intermediate Potato No. 81. Service activities use simulation and role play methods as well as discussions to increase knowledge about the material. Community service activities produce changes that are in accordance with the output targets, namely increasing students' knowledge, attitudes, and skills about First Aid with CPR (Cardiopulmonary Resuscitation) or Basic Life Support, fractures, burns, choking and electric shock.

Physical and Chemical Study of Manalagi Apple Peel (Malus sylvestris) Kombucha on 7 Days Fermentation Time: Studi Fisik dan Kimia Kombucha Kulit Apel Manalagi (Malus sylvestris) dengan Waktu Fermentasi 7 hari Kinanti Ayu Puji Lestari; Floreta Fiska Yuliarni; Widya Dara Anindya
Journal Pharmasci (Journal of Pharmacy and Science) Vol. 8 No. 2 (2023): Journal Pharmasci (Journal of Pharmacy and Science)
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v8i2.358

KKombucha was one of the millennial beverage with a lot of benefits. Kombucha was a subject of research that was always interesting to discuss because of the various indicators of its subject. One of them was the Manalagi apple peel kombucha which was very rare on reviewed. This research focuses on the physical and chemical aspects of Manalagi apple peel kombucha. Manalagi apple peel kombucha is made in three different formulations namely FKA, FKB and FKC and fermented for 7 days. Each kombucha then went through a physical test which included thickness, wet weight, dry weight, moisture content and chemical evaluation as seen from changes in the pH of the kombucha using universal pH paper. The results showed that the physical evaluation of Manalagi apple peel kombucha with the FKC formulation had the highest thickness, wet weight, dry weight and yield, while the FKB formulation had the highest water content. While the pH of each formulation of kombucha apple skin Manalagi decreased and there were differences in values on the surface, middle and bottom of each formulation from pH 5 to pH 4 and 3.

Antibacterial Potention and pH Analysis of Kombucha with Anna Apple (Malus domestica) Peel as Its Substrate Lestari, Kinanti Ayu Puji; Wulansari, Silvi Ayu
Biota Vol 15 No 1 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i1.363

This study aimed to determine the antibacterial abilities of Kombucha with Anna apple peel as the substrate. This study was conducted on the antibacterial assay of Kombucha with the formulation of 10g (formulation A), 15g (formulation B), and 20g (formulation C) apple peels. Each formulation then made three different test concentrations of an antibacterial test by the Kirby Bauer method. There was a decrease in the pH value measured before and after fermentation of kombucha apple peel due to the production of acetic acid formed from metabolic activity between bacteria and yeast as a culture of kombucha beverage. The results of the antibacterial test showed that all of the test concentrations could inhibit the growth of the test bacteria until they were in the category of medium and high inhibition. The largest diameter of the inhibition zone was shown by formula C with a concentration of 100% (17 mm). The diameter of the inhibition zone is directly proportional to the amount of apple peel contained in the formula and the concentration of the sample being tested.

Uji Daya Hambat Ekstrak Metanol Auricularia nigricans terhadap Candida spp.: Inhibitory Test of Methanol Extract of Auricularia nigricans Against Candida spp. Indrawati, Afina; Pertiwi, Amelya Dyah; Ayuningtyias, Audry Ridho; Subroto, Hillda Widianingtyas; Azizah, Mufidhatul Nur; Handayani, Tutik; Surahmaida, Surahmaida; Lestari, Kinanti Ayu Puji; Yuliarni, Floreta Fiska
Biospecies Vol. 16 No. 2 (2023): Juli 2023
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v16i2.27451

Candida was a fungus that can cause candidiasis infection. Candidiasis could occur of the skin, nails, mucous membranes and the gastrointestinal. The study aims to examine whether Auricularia nigricans methanol extract has the ability to inhibit the growth of Candida albicans, C. glabrata, and C. parapsilosis. Soxhletation was used as the extraction method, and the inhibitory test method involved diffusion paper disk and wells methods. The concentration variations used were 0.2 g/ml, 0.25 g/ml and 0.3 g/ml with 10% DMSO as a negative control. The research results obtained were that no inhibition zones were formed at concentrations of 0.2 g/ml, 0.25 g/ml and 0.3 g/ml so that they were included in the inactive category. Keywords: Auricularia nigricans, Candida, soxhlet method, methanol extract, paper disc and wells method. Â Abstrak Candida merupakan jamur golongan khamir yang dapat menyebabkan infeksi kandidiasis. Kandidiasis tersebut dapat terjadi pada kulit, kuku, membran mukosa dan saluran cerna. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak metanol Auricularia nigricans dalam menghambat pertumbuhan C. albicans, C. glabrata dan C. parapsilosis. Metode ekstraksi yang digunakan adalah sokletasi selama 10 jam dan metode uji daya hambat antifunginya secara difusi cakram dan sumuran. Variasi konsentrasi yang digunakan adalah 0,2 g/ml, 0,25 g/ml dan 0,3 g/ml dengan DMSO 10% sebagai kontrol negatif. Hasil penelitian yang diperoleh adalah tidak terbentuknya zona hambat pada konsentrasi 0,2 g/ml, 0,25 g/ml dan 0,3 g/ml sehingga termasuk dalam kategori tidak aktif. Kata kunci: Auricularia nigricans, Candida, metode sokletasi, ekstrak metanol, metode difusi cakram dan sumuran.

UJI ANGKA LEMPENG TOTAL DAN ANGKA KAPANG KHAMIR PADA Pleurotus ostreatus DAN Ganoderma lucidum Putri, Amanda Nabila; Setiawati, Asti Anwar; Masruuroh, Dina; Absani, Afriyanti Rifqi; Surahmaida, Surahmaida; Lestari, Kinanti Ayu Puji; Yuliarni, Floreta Fiska
Biospecies Vol. 18 No. 1 (2025): Januari 2025
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v18i1.36424

Mushrooms are a source of antioxidants. Mushrooms can be processed into various products. Both raw materials and finished products from mushrooms must comply with established standards. The aim of this research was to determine the total plate count and total fungal count in Ganoderma lucidum and Pleurotus ostreatus samples. The method used is the Total Plate Count (TPC) and Total Fungal Count (TFC). The TPC value in G. lucidum was 6.4 x 106 CFU/g and TFC was 3.9 x 105 CFU/g. This value exceeds the BPOM maximum standard for tea products or herbal brews. The TPC value in P. ostreatus was 1.2 x 105 CFU/g and TFC was 4.9 x 105 CFU/g. The TPC value for P. ostreatus is less than the maximum standard set by BPOM, while the TFC value exceeds the maximum standard for powder or mixture of soup and broth. The conclusion of this research is that the TPC and TFC in the G. lucidum and P. ostreatus samples exceed the standards set by BPOM No. 13 of 2019 except for the TPC in the P. ostreatus sample.

Peningkatan Keterampilan Siswa Vokasi melalui Pelatihan Pengujian Antibakteri Minuman Herbal Fermentatif dan Non Fermentatif pada Siswa SMK di Akademi Farmasi Surabaya Lestari, Kinanti Ayu Puji; Permatasari, Silfiana Nisa; Lubada, Eziah Ika; Fandinata, Selly Septy
Bangun Desa: Jurnal Pengabdian Masyarakat Vol. 4 No. 2 (2025)
Publisher : Universitas Alma Ata

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21927/jbd.2025.4(2).69-77

Indonesia dikenal kaya akan tanaman herbal yang memiliki potensi antibakteri tinggi. Untuk meningkatkan literasi sains terapan dan keterampilan vokasional siswa, telah dilakukan kegiatan pengabdian masyarakat berupa pelatihan uji aktivitas antibakteri terhadap minuman herbal fermentatif dan non-fermentatif di SMK Nur Medika Surabaya pada Mei 2025. Kegiatan ini menggunakan pendekatan praktikum laboratorium yang diawali dengan pemberian materi teori, demonstrasi, hingga praktik uji antibakteri menggunakan metode difusi cakram. Siswa dilibatkan aktif dalam setiap tahapan, mulai dari persiapan, inokulasi bakteri, hingga interpretasi zona hambat. Hasil pre-test dan post-test menunjukkan peningkatan nilai yang signifikan, dari rata-rata 40 menjadi 90. Survei kepuasan peserta juga menunjukkan respon sangat positif. Pelatihan ini terbukti efektif dalam meningkatkan pemahaman konseptual, keterampilan teknis, serta minat siswa terhadap pengembangan produk herbal sebagai alternatif antibakteri. Program ini mendukung pencapaian kompetensi vokasional dan profil pelajar Pancasila.

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