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All Journal Management Studies and Entrepreneurship Journal (MSEJ) Jurnal Ilmu Agama dan Pendidikan Agama Buddha (JIAPAB) Jurnal Pengabdian kepada Masyarakat Indo-MathEdu Intellectuals Journal Makara Journal of Science Jurnal Indonesia Sosial Sains The Indonesian Journal of General Medicine
Grace, Grace
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Pentingnya Menghargai Perbedaan dalam Masyarakat Riau Suci, Suci; Liyana, Liyana; Grace, Grace; Perawati, Perawati
Indo-MathEdu Intellectuals Journal Vol. 5 No. 6 (2024): Indo-MathEdu Intellectuals Journal
Publisher : Lembaga Intelektual Muda (LIM) Maluku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54373/imeij.v5i6.2282

Abstract

The importance of respecting differences in Riau society is crucial to create harmony and peace. The people of Riau, which are rich in culture, ethnicity, and religion, need an attitude of mutual respect so that harmony is maintained. By respecting differences, we can build good communication between individuals, reduce conflicts, and increase cooperation in various aspects of life. In addition, appreciation for differences can also enrich our experiences and insights, thereby encouraging innovation and creativity in society. This study aims to explore in depth how respecting differences can affect social stability and harmony in society. The type of research used is a qualitative approach. The data collection technique in this study uses interviews, observations, and documentation studies. Data analysis was carried out through thematic analysis and narrative analysis. The results of the analysis show that through education and open dialogue, the people of Riau can better understand the values of tolerance and mutual respect, which in turn will strengthen the unity and unity of the nation. Thus, respecting differences is not only a choice, but also a necessity to create a harmonious and prosperous society
Development of Shigella flexneri Detection Method by Real-Time Polymerase Chain Reaction Targeting the sfmD Gene Nurjayadi, Muktiningsih; Azizah, Puan Aqila; Setiawan, Agus; Sihombing, Ananda Indah Putri; Fitriyanti, Anisa; Grace, Grace; Putri, Gusti Angieta; Angelina, Helzi; Musie, Royna Rahma; Declan, Jefferson Lynford; Putri, Gladys Indira; Juliansyah, Dandy Akbar; Fatimah, Siti; Fahriza, Tiara; Kartika, Irma Ratna; Kurniadewi, Fera; Novitasari, Novitasari; Abomoelak, Bassam; El Enshasy, Hesham Ali; Chen, Shyi-Tien
Makara Journal of Science Vol. 29, No. 4
Publisher : UI Scholars Hub

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Abstract

Shigella flexneri can cause shigellosis, which results in high fever, vomiting, diarrhea, or death. This study developed a detection method based on real-time polymerase chain reaction that targets the sfmD gene of S. flexneri given that the virulence factor encoded by this gene is thought to facilitate adhesion to a host surface. The proposed method involves primer design, DNA isolation, the optimization of primer annealing temperature, and confirmation, specificity, and sensitivity tests. A sfmD primer with an amplicon length of 155 bp was annealed to the target DNA at an optimized temperature of 60 ℃ (range 54 °C–62 °C). This primer pair amplified the target sequences at a cycle threshold (Ct) of 15.11 ± 0.38 and a melting temperature of 82.41 ± 0.01 ℃. The primer specificity test showed that the primer distinguished S. flexneri from nontarget bacteria. The findings also revealed that the primer detected S. flexneri down to a limit of 16 pg/μL at a Ct of 26.68, equivalent to 2.79×102 CFU. Overall, the sfmD primer can effectively amplify S. flexneri DNA. Future research can be directed toward the detection of S. flexneri in artificially contaminated food samples to validate the real-food applicability and strengthen its potential use in food safety monitoring and clinical diagnostics.
Co-Authors Aboemolak, Bassam Abomoelak, Bassam Angelina, Helzi Azizah, Puan Aqila Berkahingrum, Ayu Chen, Shyi-Tien El Enshasy, Hesham Ali Enshasy, Hesham Ali El Fahriza, Tiara Fitriyanti, Anisa Galag, Cindy Patricia Haudi, Haudi Herwed Nelson D Jefferson Lynford Declan Juliansyah, Dandy Akbar Kartika, Irma Ratna Keren, Keren Kurniadewi, Fera Liyana, Liyana Mila Mega Muktiningsih Nurjayadi Musie, Royna Rahma Novitasari Novitasari P, Abraham Pierre Wenang Perawati Perawati, Perawati Putri, Gladys Indira Putri, Gusti Angieta Reiyan Budi Kusuma Rudy Rudy Saamia, Vira Sari, Ike Rukmana Sherlyn, Sherlyn Sihombing, Ananda Indah Putri Sinaga, Hasoloan Siregar, Al Arif Nur Siti Fatimah Stevani, Stevani Suci Suci, Suci Suryanti Trito, Belthasar Siahaan Yuli Erlina