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All Journal Biology, Medicine, & Natural Product Chemistry Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ilmiah Wahana Pendidikan Jurnal Pendidikan, Sains dan Teknologi Biocaster : Jurnal Kajian Biologi
HASAN, ANI M.
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Actinomycetes from Plant Rhizosphere in Gorontalo Karst Area as Plant Growth Promoting Rhizobacteria Matalauni, Citra Leonita; Retnowati, Yuliana; Katili, Abubakar Sidik; Kandowangko, Novri Youla; Hasan, Ani M.
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1047-1053

Abstract

This study aimed to describe Actinomycetes from the rhizosphere of plants in the Gorontalo karst area as Plant Growth Promoting Rhizobacteria (PGPR). The research method is based on a quantitative descriptive method. Soil samples from the rhizosphere were collected using a purposive sampling technique from 8 plant species at two locations in the Gorontalo karst region, namely the Tanjung Kramat Hills. The characteristics of PGPR in this study focused on phosphate solubilization ability, Indole Acetic Acid (IAA) production, antagonism against the fungus Fusarium oxysporum, and tolerance to fungicides. Potential PGPR isolates were analyzed for phylogenetic relationships based on 16S rRNA gene sequences. The results showed that there were 6 actinomycetes isolates (RzHKC-01, RzKO-02, RzO-03, RzAK-04, RzPK-05, and RzOC-06) from 8 plant species in the Gorontalo karst region. One actinomycetes isolate, RzPK-05, showed potential as a PGPR with the ability to solubilize phosphate and produce IAA at 0.165 mg/L and 0.133 mg/L, respectively. Isolate RzPK-05 exhibited antagonistic properties against Fusarium oxysporum with an inhibition rate of 82.24% and was tolerant to fungicides such as Captive, Benlate, and Thiramo up to a concentration of 100 mg/L. Molecular analysis based on the 16S rRNA gene and phylogenetic tree reconstruction indicated that the RzPK-05 isolate is closely related to the genus Streptomyces with 100% similarity.
Isolasi, Identifikasi, dan Uji Efektivitas Metabolit Sekunder Bakteri Endofit Sembung (Blumea balsamifera) Sebagai Biopestisida Terhadap Kutu Kebul (Bemisia tabaci) Timumun, Radina I.; Agustina, Harista; Hasan, Ani M.; Uno, Wirnangsi D.; Retnowati, Yuliana
Jurnal Pendidikan, Sains Dan Teknologi Vol. 5 No. 1 (2026): Januari - Maret
Publisher : CV. ITTC INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47233/jpst.v5i1.4223

Abstract

Blumea balsamifera contains bioactive metabolites that are presumed to support the presence of endophytic bacteria capable of producing secondary metabolites with pesticidal potential, making this plant a promising source of eco-friendly biopesticides. This study aimed to isolate and identify endophytic bacteria associated with B. balsamifera and to evaluate the insecticidal activity of their secondary metabolites against Bemisia tabaci. Endophytic bacteria were isolated using the Direct Planting and Grinding methods, followed by purification, Gram staining, and metabolite production through liquid culture in Luria-Bertani (LB) medium. The isolated strain was identified as a Gram-negative, short rod-shaped bacterium producing a yellowish supernatant, which was successfully extracted using ethyl acetate to obtain an extracellular metabolite fraction. Toxicity bioassays revealed a clear dose–response pattern, with 24-hour mortality of B. tabaci increasing from 55.00% at 35% concentration to 80.00% at 75% concentration. Probit analysis yielded an LC₅₀ value of 1500.95, while LT₅₀ decreased significantly from 42.26 hours to 15.87 hours as metabolite concentration increased. These findings confirm that endophytic bacteria isolated from B. balsamifera are capable of producing secondary metabolites effective in suppressing B. tabaci populations, highlighting their strong potential for development as environmentally friendly plant-based biopesticides.
Analisis Faktor Internal Penyebab Kesulitan Belajar Ditinjau dari Motivasi Belajar dan Minat Belajar Latief, Sulastri; Hasan, Ani M.; Mustaqimah, Nur
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 1 (2026): January
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i1.954

Abstract

This study aims to analyze internal factors causing students' learning difficulties in biology, specifically from the perspective of motivation and interest. The study was conducted at SMA Negeri 2 Gorontalo, with 29 students in grades XI-3 in the 2025/2026 academic year as subjects. The method used was descriptive quantitative, with data collection techniques using questionnaires, observations, and interviews. The results showed that students' learning motivation was high, with an average percentage of 78%, while their learning interest was also high, with an average percentage of 79%. However, learning difficulties were still found in some students, influenced by low self-discipline and a lack of independent study habits outside of school hours. These findings indicate that high motivation and interest in learning are not fully accompanied by effective and consistent learning behaviors. Therefore, learning efforts are needed that focus not only on increasing motivation and interest but also on strengthening students' learning independence and discipline to minimize learning difficulties in biology.
Co-Authors Abubakar Sidik Katili Agustina, Harista Elya Nusantari Frida Maryati Yusuf Hamidun, Marini S. Herinda Mardin Ismail, Lilis U. Jusna Ahmad Kartika, Dika Lamangida, Novi Safitri Latief, Sulastri Lilan Dama Mabuia, Siti Ainun Mamu, Hartono Margaretha Solang Masra Latjompoh Matalauni, Citra Leonita Moha, Siti Nurliana Muh. Nur Akbar Mursali, Putri Regina Mustamin Ibrahim Mustaqimah, Nur Novri Youla Kandowangko Nurain, Saleh Pobela, Juliani Renowati, Yuliana Rinaldi Rinaldi Syam S. Kumaji Timumun, Radina I. Uno, Wirnangsi D. Uno, Wirnangsih Din Wirnangsih D. Uno Yuliana Retnowati Yusuf, Septia