. Giyanto
Department Of Plant Protection, Faculty Of Agriculture, IPB University Jln. Kamper, Kampus IPB Dramaga, Bogor, West Java 16680

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Journal : Jurnal Ilmu Pertanian Indonesia

Kajian pemanfaatan limbah organik cair untuk pembiakan masal agens antagonis pseudomonas flourescens serta uji potensinya sebagai bio-pestisida . Giyanto; Efi Toding Tondok
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 2 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Pseudomonas fluorescens has been well known as biological control agent for plant diseases control. The ;:>roblem to apply the agents widely in the field or in the level of farmer is limited technology of mass production Nith low cost, due to the simple technology of propagation has not been yet available. The objective of this research is to study the potency of liquid organic wastes as media for mass production of P. f/uorescens and to formulate them as bio-pesticide. The results showed that modification of coconut water to pH of 7.0 could be used as media for growing P. flourescens. The P. fluorescens also could grow well in livestock liquid waste by adding 10% meat extract. On the other hand, the liquid tofu waste and liquid compost waste became good media for growing of P. fluorescens by addition of 10°/o meat extract and 1.2S0/o sugar. Tetes tebu will be very good media for P. f/uorescens at S% final concentration and by adding of 10°/o meat extract and 2.S0/o of sugar. The P. fluorescens showed high antagonistic effect to Ralstonia solanacearum and Sclerotium rolfsii in all of modified liquid organic wastes media. Survival and antagonisctic activity of P. fluorescens in modified organic liquid wastes stored at S°C or room temperature were 12 weeks. In vivo antagonistic and plant growth promoting activity showed that P. fluorescens grown in liquid organic waste suppressed the incidence of stem rot diseases caused by Sclerotium rolfsii and increased the vigor of plant growth on watermelon. Formulation of the P. Fluorescens grown in modified coconut water gave the best performance of P. fluorescens in supppressing of plant diseases and inducing plant growth. The product of BeMOR(e) (beneficial microorganism) from the result of this research will be proposed to be patented.
EVALUASI KEMURNIAN GENETIK DENGAN MARKA MIKROSATELIT DAN APLIKASI RIZOBAKTERIA UNTUK MENINGKATKAN PRODUKSI DAN MUTU BENIH JAGUNG HIBRIDA Memen Surahman; . Giyanto; Andi Takdir; Awaludin Hipi
Jurnal Ilmu Pertanian Indonesia Vol. 17 No. 1 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

One effort to improve of high-quality of maize seed were the development and application of methods for genetic quality testing, such as SSR marker. Another effort was used of rhizobacteria for increased the availability of nutrients, especially P in the soil. The aim of this study were: 1) Microsatellite markers specific to male and female parents of maize hybrid; 2) Seed genetic purity by using molecular marker; 3) Rhizobacteria that could increase the physiological quality of maize hybrid seed; and 4) Rhizobacteria that could increase the growth of plant and efficiency of fertilizer P. The result showed that: 1) From five markers tested, three markers namely phi96100, phi328175 and phi072 produced polymorphic bands and capable to distinguish parental lines of two maize hybrids. Microsatellite marker phi96100 was specific used for testing genetic purity of cv.Bima-4 and phi072 for cv.Bima-3. While phi328175 was specific markers to both hybrids maize. The test of cv. Bima-3 and Bima-4 indicated that genetic purity of both varieties were 97.5% and 80% respectivelly; 2) Isolates B28 and B46 could increase IV 19% and 22% respectively, and had a high speed of growth compared controls; 3). Isolates B46, B42, B13, P14, P31, AB2, AB3, AB11, ATS4, and ATS5 could increased of germination compared to control; 4) Treatment of rhizobacteri significantly affects on plant height 2, 4 and 6 week after planting (WAP); 5) P fertilizer dosage were not significantly influenced on the number of leaves at 2 and 4 WAP, but significant at 6 WAP. Isolate of B28, B42 and ATS4 were potential for increased of plant growth. 
Pengaturan Arsitektur Tanaman untuk Menyeimbangkan Sink dan Source serta Meningkatkan Pertumbuhan dan Hasil Kacang Koro Pedang Abdullah Sarijan; Memen Surahman; Asep Setiawan; Giyanto Giyanto
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 1 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (580.181 KB) | DOI: 10.18343/jipi.25.1.43

Abstract

The research to improve the growth, production, and seed quality of Jack Bean (Canavalia ensiformis L.) through pruning, which was carried out from May to October 2016 in Purwasari Village, Dramaga, Bogor Regency and continued by seed testing at the Seed Testing Laboratory, Bogor Agricultural University in December 2016. The research was designed in a randomized block design (RBD) with eight treatment of stem and branch pruning and one treatment without pruning as a control, i.e. without pruning of branches and stems (P1), pruning on the 10th stem nodes (P2 ), pruning on the 11th stem nodes (P3), pruning on the 5th branch nodes (P4), pruning on the five branch nodes and the 10th stem nodes (P5), pruning on the 5th branch nodes and stem nodes to -11 (P6), pruning on the 6th branch nodes (P7), pruning on the 6th branch nodes and 10th stem nodes (P8), and pruning on the 6th branch nodes and 11th stem nodes (P9). The results showed that pruning affected some of the results of the study, but was not able to increase the production of Jack Bean. Pruning treatment on the 5th branch nodes and 10th stem nodes (P5) produced the highest seed production (3.4 tons ha-1), the physical quality of the seeds produced was classified as moderate with a weight of 1000 seeds between 1273.3-1291.7 g. The physiological quality of the seed is moderate with maximum growth potential 84.0-90.7%, germination percentage 73.3-82.7%, germination speed 9.1-116.1% / etmal, germination uniformity 40.0-49 , 3% and vigor index of 14.0-20.0%. Keywords: germination rate, inflorescence, oas bakol, pod
Bacillus endophyticus: Symbiotic Bacterium in Subterranean Termites Intestine (Blattodea: Termitoidae) from Bogor, Indonesia Nadzirum Mubin; Giyanto Giyanto; Idham Sakti Harahap
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 2 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.2.191

Abstract

Rayap merupakan serangga sosial yang berperan penting dalam perputaran siklus nutrisi. Di dalam sistem pencernaan rayap, terdapat simbion yang membantu proses degradasi selulosa. Penelitian ini bertujuan mengisolasi bakteri simbion yang terdapat di dalam saluran cerna rayap tanah. Penelitian diawali dengan koleksi rayap tanah di Kampus IPB University, diikuti isolasi bakteri simbion dari saluran cerna belakang (proktodeum) yang kemudian diidentifikasi berdasarkan morfologi, fisiologi, dan molekuler menggunakan gen 16S rRNA. Enam rayap tanah yang diperoleh adalah Macrotermes gilvus, Odontotermes javanicus, Microtermes insperatus, dan Capritermes mohri (Famili Termitidae); Schedorhinotermes javanicus dan Coptotermes curvignathus (Famili Rhinotermitidae). Dari enam rayap diperoleh 43 isolat dan satu isolat yang memiliki karakter umum. Isolat bakteri kode 8A_27F berwarna putih, elevasi seperti tombol dengan tepian licin. Dari uji fisiologis, isolat ini termasuk ke dalam bakteri Gram positif, berspora, dan bersifat aerob. Identifikasi dengan gen 16S rRNA menunjukkan bahwa isolat bakteri tersebut memiliki tingkat kemiripan sebesar 98% dengan spesies Bacillus endophyticus. Kata kunci: bakteri, rayap, 16S rRNA, simbion, proktodeum