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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI)
. Giyanto
Department Of Plant Protection, Faculty Of Agriculture, IPB University Jln. Kamper, Kampus IPB Dramaga, Bogor, West Java 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : Jurnal Perlindungan Tanaman Indonesia

 1 
Morphological and Molecular Characteristics of Pratylenchus coffeae from the Origin of Robusta Coffee Plantation in Malang, East Java Aris Budiman; Supramana Supramana; Giyanto Giyanto
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.42481

Abstract

Pratylenchus coffeae is the most important plant-parasitic nematode in Robusta coffee plantations. Information regarding morphology, morphometric and molecular characters of P. coffeae has not been reported in Indonesia. This study is aimed to describe those characters of P. coffeae that attack Robusta coffee. Root samples were taken from Robusta coffee plantation in Malang, East Java. Nematode extractions was conducted using a mist chamber method. Morphology and morphometric characters were observed from the permanent nematode slides. Single nematode DNA extract was amplified at the D2D3 segment of 28S rRNA and ITS1-5.8S-ITS2 rRNA with universal primers. Amplicon was sequenced and analysed for phylogenetic tree relationships. Female morphological key character of P. coffeae observed are: lip with two annulations, four lateral lines, esophageal overlap with intestine ventrally, monodelphic, and truncated tail shape. Male spicules curved ventrally. Female morphometrics are: n=26, L = 556.4 μm, DGO = 2.4 μm, anterior gonad = 174.8 μm, a = 28.5, b = 6.1, b’ = 4.1, c = 20.1, c’ = 2.3, V = 81.7. A Male is smaller than a female with n=24, L = 505.9 μm,  a = 32.3, b = 5.5, b '= 3.9, c = 15.3, c' = 2.8 and T = 40.6. The molecular characters of P. coffeae were investigated for two isolates, namely SA1 and SA2.  Based on the D2D3 and ITS1-5.8S-ITS2 regions, isolate SA1 has similarity level of 99% and 97% to the P. coffeae  from NCBI. Similar result was shown by Isolate SA2 with similarity of 100% and 100% respectively. Phylogenetic tree analysis using Maximum Likelihood at the D2D3 segment of 28S rRNA and ITS1-5.8S-ITS2 regions showed that P. coffeae in this study was included in one clade with P. coffeae from several countries.
Phytonematodes Community and Polyphasic Character of Aphelenchoides varicaudatus on Garlic Plants in Tegal Regency, Central Java Mokhammad Danang Kusuma; Supramana Supramana; Giyanto Giyanto
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.49779

Abstract

The interception of Ditylenchus dipsaci, D. destructor, and Aphelenchoides fragariae, which are classified as quarantine pests, has been reported to occur on imported garlic bulbs used for consumption at several traditional markets in Bogor. This finding should increase awareness of the chance of garlic contamination in Indonesia’s garlic fields. This study aimed to evaluate the nematode community and determine polyphasic characters of Aphelenchoides species from garlic plantations. The study was conducted by sampling soil and plants from garlic plantations in Tuwel and Rembul Villages, Bojong District, Tegal Regency, Central Java in December 2018. Nematode extraction was done using a mist chamber for plant materials and sugar flotation – centrifugation methods for soil samples. Parameters measured included nematode species identity, absolute population and prominence indexes. Nematode identification was conducted based on their morphological characters. Further identification based on morphometric and molecular characters (polyphasic) conducted for nematodes species suspected as quarantine pests. Six generas of nematodes identified were Helicotylenchus, Aphelenchoides, Rotylenchulus, Aphelenchus, Criconemoides, and Tylenchus. Aphelenchoides sp. had the highest prominence value from plant tissue with a value of 6.32 and is categorized as a quarantine pest genus (A. fragariae) in garlic and was further identified to the species level. Further identification based on polyphasic characters showed the Aphelenchoides sp. found was A. varicaudatus.
Co-Authors Abdjad Asih Nawangsih Agus Eko Prasetyo Aisyah Imas ALI NURMANSYAH Andi Takdir Anwar, Aswaldi Aprizal Zainal Aris Budiman Awaludin Hipi Bambang Nuryanto Dadang Dadang DAMAYANTI BUCHORI Dori Kusuma Jaya DWI ANDREAS SANTOSA Efi Toding Tondok Elvina Efendi Eny Widajati Erina Sulistiani Gustan Pari Gustan Pari Haliatur Rahma, Haliatur Herliyana Indahwardani Idham Sakti Harahap Imas Aisyah Indahwardani, Herliyana Kikin H Mutaqin Kikin H. Mutaqien Kresnamurti Kurniasih Leiwakabessy, Christoffol Mahardika Gama Pradana Meity S Sinaga, Meity S Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Memen Suharman, Memen Memen Surahman Mokhammad Danang Kusuma Mubin, Nadzirum Muhammad Evan Nurrahmawan Muhammad Yasin Sonhaji Novik Nurhidayat RAHAYU WIDYASTUTI Ratna Sari Dewi Sanjaya, Wilhelmus Terang Arga Sarah Desmia Muchtar Sarah Desmia Muchtar Sarijan, Abdullah - Sarjiya Antonius Satoshi Nakamura SATRIYAS ILYAS Seiichi Furukawa Setiawan, Asep S Sudarsono Supramana Syaiful Khoiri TRI ASMIRA DAMAYANTI Trikoesoemaningtyas Tuminem ., Tuminem