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All Journal Jurnal Biologi Tropis INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Indonesian Journal of Pharmacy and Natural Product Academic Hospital Journal Jurnal Pengabdian Masyarakat dan Riset Pendidikan
Fuzianingsih, Eka Noviya
STIKes Karya Putra Bangsa
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Potensi Antioksidan Biji Pepaya terhadap Parameter Oksidatif Jantung pada Tikus Jantan Model Diet Tinggi Lemak: Antioxidant Potential of Papaya Seeds on Cardiac Oxidative Parameters in Male Rats with High Fat Diet Model Arif Santoso; Choirul Huda; Sulastri; Pera Amelia; Rissa Laila Vifta; Melati Aprilliana Ramadhani; Eka Noviya Fuzianingsih
Indonesian Journal of Pharmacy and Natural Product Vol. 8 No. 01 (2025): Indonesian Journal of Pharmacy and Natural Product
Publisher : Universitas Ngudi Waluyo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35473/ijpnp.v8i01.3836

Abstract

An imbalance between the body's antioxidant system capacity and free radical production can lead to oxidative stress, which ultimately causes the lipid peroxidation process. This phenomenon is commonly identified by increased superoxide dismutase (SOD) activity and reduced malondialdehyde (MDA) concentrations. The objective of this study was to assess the efficacy of ethanolic papaya seed (Carica papaya L.) extract in modulating SOD and MDA levels in the heart tissue of male Sprague-Dawley rats subjected to a high-fat diet over 17 days. A total of 30 rats were used and allocated into six groups: a positive control (K+), a negative control (K−), a standard control (KN), and three treatment arms receiving oral doses of 150 mg/kgBW (P1), 250 mg/kgBW (P2), and 350 mg/kgBW (P3). The extraction process employed 70% ethanol through maceration. The lowest MDA average was recorded in the P3 group (3.50±1.65), whereas the K-group exhibited the highest level (24.45±4.71). In terms of SOD, the K+ group had the highest mean (65.46±5.93), and the K− group the lowest (36.34±7.57). Statistical analysis using Tukey’s test showed that administration of 350 mg/kg body weight extract yielded results closely resembling those of the positive control. However, the observed differences did not reach statistical significance (p > 0.05). The conclusion is that administration of papaya seed extract at a dose of 350 mg/kgBW has the potential to reduce MDA levels and increase SOD levels, with effects similar to positive controls.   ABSTRAK Tidak seimbangnya antara kapasitas sistem antioksidan tubuh dan produksi radikal bebas dapat mengakibatkan kearah stres oksidatif, yang pada akhirnya menyebabkan proses peroksidasi lipid. Keadaan ini ditandai oleh aktivitas enzim superoxide dismutase (SOD) yang meningkat dan konsentrasi malondialdehida (MDA) yang menurun. Penelitian ini bertujuan mengevaluasi potensi ekstrak etanol biji pepaya (Carica papaya L.) dalam memodulasi kadar SOD dan MDA pada jaringan jantung tikus jantan galur Sprague Dawley yang diberi diet tinggi lemak pada rentang waktu 17 hari. Penelitian ini memakai desain eksperimental dengan melibatkan 30 ekor tikus, yang dikelompokkan menjadi enam: kelompok kontrol positif (K+), kontrol negatif (K−), kontrol normal (KN), serta tiga kelompok perlakuan dengan dosis bertingkat yaitu 150 mg/kgBB (P1), 250 mg/kgBB (P2), dan 350 mg/kgBB (P3). Ekstrak diperoleh melalui metode maserasi menggunakan pelarut etanol 70%. Hasil analisis menyatakan bahwa kadar MDA terendah ditemukan pada kelompok P3 (3,50±1,65), sedangkan kadar tertinggi terdapat pada kelompok K− (24,45±4,71). Sebaliknya, kadar SOD tertinggi dicatat pada kelompok K+ (65,46±5,93) dan terendah pada kelompok K− (36,34±7,57). Berdasarkan uji lanjut Tukey, pemberian ekstrak biji pepaya dosis 350 mg/kgBB (P3) memberikan hasil yang secara statistik mendekati kelompok kontrol positif, meskipun belum menyatakan perbedaan bermakna (p > 0,05). Kesimpulannya adalah pemberian ekstrak biji pepaya dosis 350 mg/kgBB memiliki potensi pada penurunan kadar MDA dan meningkatkan kadar SOD, dengan efek yang menyerupai kontrol positif.
The Role of TNF-α (Tumor Necrosis Factor-alpha) in Pulmonary Tuberculosis Sulistyasmi, Wiwit; Fuzianingsih, Eka Noviya; Kuncara, Rachmad Bayu
Academic Hospital Journal Vol 7, No 2 (2025)
Publisher : Rumah Sakit Akademik Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ahj.v7i2.106190

Abstract

Background: Tuberculosis (TB) remains a primary global health concern, with Indonesia accounting for approximately 10% of international cases. Tumor necrosis factor-alpha (TNF-α) plays a crucial role in the immune response against Mycobacterium tuberculosis, particularly in pulmonary tuberculosis (TB).Method: This article is a narrative literature review that examines the role of tumor necrosis factor-alpha (TNF-α) in pulmonary tuberculosis.Result:  This pro-inflammatory cytokine is essential for activating macrophages, promoting phagolysosome fusion, and supporting the formation and maintenance of granulomas—immune structures critical for containing infection. However, dysregulated TNF-α expression can be detrimental: excessive levels contribute to inflammation and lung tissue damage, while insufficient production—such as that induced by TNF-α inhibitor therapy—can lead to reactivation of latent TB. This narrative review examines the biosynthesis, immunological functions, and regulatory mechanisms of TNF-α in the context of pulmonary tuberculosis, highlighting its dual role in protective immunity and immunopathology.Conclusion: A deeper understanding of TNF-α modulation may inform the development of targeted, host-directed therapies that improve treatment outcomes while minimizing tissue damage.
Antibacterial Activity of Red Ginger (Zingiber officinale) Extract Against Diarrhea Causing Bacteria Using the Well Diffusion Method Fuzianingsih, Eka Noviya; Rahmawati, Ayu Sintya Citra; Safitri, Yunita Diyah
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10911

Abstract

Acute diarrhea resulting from bacterial infections remains a significant concern in public health. The improper or excessive use of antibiotics contributes to the emergence of antibiotic resistance, creating a need for safer therapeutic alternatives derived from natural sources, such as red ginger (Zingiber officinale). This study aimed to evaluate the antibacterial potential of red ginger extract against Escherichia coli, one of the common bacterial agents causing diarrheal disease. The assessment was carried out using the well-diffusion technique by observing and measuring the diameter of the inhibition zones produced by the extract. The extract was tested at concentrations of 40%, 60%, and 80%, accompanied by a positive control (ciprofloxacin 1 mg/mL) and a negative control (distilled water). The findings demonstrated that the positive control produced a mean inhibition zone of 44.375 mm, whereas the negative control showed no inhibitory activity. The 40% extract yielded an average inhibition zone of 17.125 mm, the 60% concentration produced 19.625 mm, and the 80% concentration generated an average diameter of 22 mm. Statistical evaluation using one-way ANOVA indicated a significance level of ≤ 0.05, and the Duncan post hoc analysis confirmed significant differences between all extract concentrations compared to the antibiotic control. Overall, the study demonstrated that red ginger extract possesses substantial antibacterial activity and effectively suppresses the growth of diarrhea-causing E. coli through the well-diffusion approach.
Optimalisasi Pemanfaatan Tanaman Bunga Telang dan Madu Sebagai Produk Minuman Kaya Antioksidan di Desa Joho: Pengabdian Sulastri; Arif Santoso; Nurul Chamidah Kumalasari; Eka Noviya Fuzianingsih; Rissa Laila Vifta; Melati Aprilliana Ramadhani
Jurnal Pengabdian Masyarakat dan Riset Pendidikan Vol. 4 No. 4 (2026): Jurnal Pengabdian Masyarakat dan Riset Pendidikan Volume 4 Nomor 4 April - Juni
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jerkin.v4i4.6141

Abstract

Bunga telang (Clitoria ternatea) merupakan tanaman bunga yang banyak tumbuh di pekarangan warga desa Joho, Tulungagung. Tanaman ini memiliki banyak sekali manfaat untuk kesehatan tubuh salah satunya yaitu sebagai sumber antioksidan. Bunga telang dan madu dicampur dan diolah menjadi minuman kaya antioksidan yang memiliki banyak manfaat. Kegiatan pengabdian dilaksanakan untuk memberikan pedampingan dalam usaha pemanfaatan bunga telang dan memberikan edukasi kepada warga sekitar agar dapat mengolah dan memanfaatkan bunga telang secara optimal. Kegiatan yang dilakukan meliputi pemberian sosialisasi kepada warga mengenai manfaat bunga telang sampai menjadi teh bunga telang dan memberikan edukasi cara pembuatan minuman dari teh bunga telang dari penyeduhan hingga minuman jadi. Pelaksanaan kegiatan pengabdian menghasilkan adanya peningkatan yang terukur dalam pemahaman pengetahuan warga mengenai khasiat dan manfaat bunga telang sebagai minuman kaya antioksidan dari cara penanaman hingga pengolahan menjadi teh bunga telang, menghasilkan minat dalam upaya pembudidayaan bunga telang dan munculnya inisiatif untuk dapat mempromosikan hasil produk sediaan teh bunga telang sehingga dapat memberikan manfaat dari segi kesehatan dan sosial-ekonomi.
Diagnostic Accuracy of ELISA Compared to Rapid Diagnostic Tests for Toxoplasmosis: A Systematic Review Eka Noviya Fuzianingsih; Wiwit Sulistyasmi; Rachmad Bayu Kuncara; Sulastri Sulastri
Academic Hospital Journal Vol 8, No 1 (2026)
Publisher : Rumah Sakit Akademik Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ahj.v8i1.112818

Abstract

Background: Toxoplasma gondii is an obligate intracellular protozoan responsible for toxoplasmosis, a globally prevalent parasitic infection affecting more than one-third of the world’s population. Accurate and timely diagnosis is crucial, particularly for pregnant women and immunocompromised individuals, to prevent severe complications such as congenital toxoplasmosis and encephalitis. The enzyme-linked immunosorbent assay (ELISA) is widely regarded as a reference serological method due to its high sensitivity and specificity. In contrast, rapid diagnostic tests (RDTs) offer practical advantages but show variable diagnostic performance.Method: This systematic review evaluated and compared the diagnostic accuracy of ELISA and RDTs for toxoplasmosis. Literature searches were conducted in PubMed and Google Scholar for studies published between 2015 and 2025. Eligible studies assessed the diagnostic performance of ELISA and RDTs for detecting T. gondii infection in human populations, including sensitivity, specificity, and overall accuracy.Result: Five studies met the inclusion criteria. Across diverse populations, ELISA consistently demonstrated high diagnostic accuracy, with sensitivity and specificity exceeding 90% in most studies. In contrast, RDTs showed lower performance and greater heterogeneity, particularly in individuals with low antibody titers and in immunocompromised individuals. Nevertheless, RDTs provided operational advantages for rapid screening in low-resource settings.Conclusion: ELISA remains the most reliable method for serological diagnosis of toxoplasmosis, while RDTs may serve as complementary tools for initial screening. The integration of both approaches, along with the adoption of advanced ELISA formats such as recombinant antigen-based or Nano-Gold ELISA, may improve early detection and enhance diagnostic capacity across different healthcare settings.
Effects of goat bile treatment on parasitemia, survival, and parasite-specific IgG in Plasmodium berghei-infected BALB/c mice Heny Arwati; Cyuzuzo Callixte; Eka Noviya Fuzianingsih; Marselaonety La’lang
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 8 No 2 (2026): Diagnostic Development Must Encompass All Stages of Testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v8i2.8033

Abstract

Malaria remains a major global health problem. Immunoglobulin G (IgG) plays a key role in naturally acquired immunity and is associated with disease progression. Goat bile (GB) has traditionally been used as an antimalarial remedy; however, its effects on immunity and the balance between its efficacy and toxicity remain unclear. This study evaluated the effect of GB on anti-Plasmodium berghei IgG levels and their association with parasitemia and survival in infected mice. Fifty male mice were divided into five groups (n = 10 per group) and infected with P. berghei. Three groups received GB at 25% (GB25), 50% (GB50), and 100% (GB100). The positive control group (POS) received dihydroartemisinin–piperaquine (DHP: 187.2 mg/kg body weight), and the negative control group (NEG) received sterile water. Parasitemia was monitored daily, and IgG levels were measured by indirect ELISA (OD450) after 4 days of treatment. GB100 produced the lowest parasitemia (16.76% ± 1.41) but reduced survival (60%). GB25 and GB50 showed moderate parasitemia (35.80% ± 4.03 and 31.21% ± 4.31, respectively) with 100% survival. IgG levels were highest in the untreated mice (NEG) group (3.70 ± 0.21) and significantly lower in all treated groups (p < 0.01). GB demonstrated anti-plasmodial activity, with greater parasite suppression at higher concentrations. However, GB100 was associated with reduced survival and diarrhea, indicating treatment-related toxicity. Lower parasite burden was accompanied by lower anti-P. berghei-specific IgG levels. These findings suggest that parasite suppression, survival, and parasite-specific IgG responses should be considered together when evaluating the therapeutic potential of goat bile.
Co-Authors Arif Santoso Arif Santoso Choirul Huda Cyuzuzo Callixte Heny Arwati Kuncara, Rachmad Bayu Marselaonety La’lang Melati Aprilliana Ramadhani Melati Aprilliana Ramadhani, Melati Aprilliana Nurul Chamidah Kumalasari Pera Amelia Rachmad Bayu Kuncara Rahmawati, Ayu Sintya Citra Rissa Laila Vifta Safitri, Yunita Diyah SULASTRI Sulastri Sulastri Sulastri vifta, rissa laila Wiwit Sulistyasmi Wiwit Sulistyasmi