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All Journal Journal of Food and Pharmaceutical Science Jurnal Ilmiah Farmasi Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Prosiding Seminar Biologi Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Pharmaciana: Jurnal Kefarmasian STOMATOGNATIC- Jurnal Kedokteran Gigi Indonesian Journal of Biotechnology INDONESIAN JOURNAL OF PHARMACY JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Syntax Literate: Jurnal Ilmiah Indonesia
Sismindari .
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Social Sciences Other

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Morphological And Biochemical Responses Of Saccharum Spontaneum L. Accessions To Drought Stress Munawarti, Aminatun; Taryono, Taryono; Semiarti, Endang; Sismindari, Sismindari
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Glagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germplasm has not been extensively used in breeding programs, primarily in relation to improve drought tolerance. The objectives of this experiment were to evaluate the effect of drought stress initiated at vegetative growth stage on growth, leaf proline content and protein pattern of seven glagah accessions (BOT-53, BOT-54, BOT-60, BOT-77, BOT-78, BOT-84, and BOT-88). The plants were propagated from single node stalk segments in polybag in the field under non-stress condition for two months. The two month-old plants were then subjected to drought stress by withholding watering for eight weeks. Untreated control plants were watered every two days. Results indicated that drought stress reduced plant height, stalk diameter and green leaf number. On the other hand, there was a little difference between drought-stressed and control plants in terms of proline content. The protein pattern showed that drought stress caused a change in gene expression in the form of induction or repression of protein expression. A specific protein with a low range of molecular weight (Rf value about 0.647) showed constitutively expressed in accession BOT-53 but drought-inducible expressed in BOT-54. Keywords: Drought stress, glagah, proline, protein pattern, Saccharum spontaneum
Co-Authors A. Suratman Achmad Fudholi Aditya K. Karim Aditya Krishar Karim Aminatun Munawarti, Aminatun Arifin, Muhammad Fajar Asih Triastuti Edy Meiyanto Endang Astuti Endang Semiarti Fortunella Tjondro Indah Purwantini Istriyati ., Istriyati J. O. Ughude Lina Winarti Mariyani Mulia, Muhammad Adi Ni Putu Linda Laksmiani Nining Sugihartini Puji Astuti Purwanto Purwanto Putu Riana Suastari Rahayu, Putu Riana Suastari Rasyidah, Muthi'ah Retno Sunarminingsih Sudibyo Ritmaleni, Ritmaleni Ronny Martien Rumiyati, Rumiyati Shanti Listyawati Sofia Mubarika Haryana Sudjadi ., Sudjadi Suwidjiyo Pramono Taryono, Taryono Tri Ratnaningsih Umar Anggara Jenie Widya Asmara Yosi B. Murti Zulies Ikawati, Zulies