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Indikator Kesehatan Ikan Kerapu Cantik (Epinephelus sp.) Yang Terdapat Pada Budidaya Keramba Pantai Timur Pangandaran Kartiawati Alipin; Tresna Aulia Sari
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p18

Abstract

Physiological changes caused by environmental stress and associated health status can be detected by haematological levels. This study aims to determine the health status of groupers (Epinephelus sp.) in the East Coast cage Pangandaran. The method used is observation method which includes the observation of location, sampling of fish, and interview with keramba owner and direct observation in the laboratory which includes identification of fish species, visual observation of fish, blood sampling, calculation of erythrocyte count, leucocytes, and measurement of hemoglobin . The data obtained were analyzed descriptively qualitatively presented in the form of tables and drawings. Observations showed that beautiful groupers (Epinephelus sp.) were the most cultivated fish and one of the most popular fish. This grouper has an erythrocyte count of 2.31 x 106 ± 1.79 cells / mm3, a leukocyte count of 226,325 x 103 ± 0.23 cells / mm3, and hemoglobin levels of 9.8 ± 2.8 gram%. Based on these data, it can be concluded that there is the beautiful grouper fish as unhealthy which cultivated in East Coast cages Pangandaran because the number of leukocytes that exceed the normal range and low hemoglobin levels. Keywords: Hematology, Grouper, East Coast Pangandaran
Suplementasi Probiotik dan Temulawak pada Ayam Pedaging terhadap Populasi Salmonella sp dan kolesterol darah (PROBIOTICS AND TEMULAWAK SUPPLEMENTATION ON BROILER CHICKENS AGAINST SALMONELLA SP POPULATION AND BLOOD CHOLESTEROL LEVEL) Kartiawati Alipin; Ratu Safitri; Ruhyat Kartasudjana
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (89.496 KB)

Abstract

Probiotics is a live microbial feed supplements that improve the microbial balance in the digestivetract of cattle host. Temulawak (Curcuma xanthorrhiza Roxb) is one of native Indonesian spices that areuseful for prevention and treatment of human disease but also can be used for broiler chickens supplement.Bioactive contents known acting as an anti-bacterial, anti-inflammatory, and anti-fungal. The study wasconducted to determine the effects of probiotic and temulawak supplementation on population of Salmonellasp and broiler chickens blood cholesterol. This study used 300 DOC final stock. Probiotic concentration 109cell/mL administered through drinking water every day while of temulawak extract dose 0.25 g/L 0.50 g/Lonce a week, for five weeks. The variables measured were Salmonella sp populations and blood cholesterollevels. The results showed that combination administered of probiotics with temulawak dose 0.50 g/Ldecreased of population of Salmonella amount to 87.04% and decreased of blood cholesterol amount21,48% at administered of probiotics with temulawak dose 0,25 g/L. The conclusions of this study showedthat the percentage decrease of Salmonella sp population and blood cholesterol levels in combinationprobiotic and temulawak supplementation so that broiler produced is safe for consumption.
Ekstrak Etanol Kulit Buah Jengkol Menurunkan Kadar Glukosa dan Meningkatkan Hormon Insulin Tikus Diabetes Yang Diinduksi Streptozotocin Desak Made Malini; Madihah Madihah; Dita Aprila Khoirunnisa; Indriani Sasmita; Nining Ratningsih; Kartiawati Alipin; Wawan Hermawan
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.755 KB) | DOI: 10.19087/jveteriner.2019.20.1.65

Abstract

Diabetes Mellitus (DM) is a disease with metabolism disorder signed by increasing of glucose blood (hyperglicemia), caused by disorder of insulin secretion and or increasing of insulin resistance. Therapy of jengkol’s fruit peel is an alternative treatment for DM. This study aims to determine the effect of ethanol extract of jengkol’s fruit peel to decrease glucose and increase insulin level of Wistar female rats which induced by streptozotocin (STZ). This research used the experimental method of Completely Randomized Design (RAL). Rat were first induced by single intravenous injection of STZ except Negative Control. Furthermore, rats were divided into six groups, namely Negative Control (NC), Positive Control (PC), Reference Group (RG) glibenclamide 10 mg/kg BW, and ethanol extract of jengkol’s fruit peel (EEOJFP) treatment at dose of 385 (P1), 770 (P2), and 1540 (P3) mg/kg BW with four replications each. Treatment was administered orally for 14 days. Data were analyzed by analysis of variance (ANAVA) with 95% confidence level and Duncan Multiple Range Test. The result showed that treatment of EEOJFP with dose of 385, 770 and 1540 mg/ kgBW decreased blood glucose 28.6%;65.4% and 59.1% successively, increased insulin as 54%; 230% and 206%. There was a protective effect of EEOJEF to level of blood glucose and insulin secretion of rat-STZ induced. The conclusion from this research is Ethanol extract of Jengkol’s fruit peel at dose 770 mg/kg BW is an effective dose in decreasing the level of glucose and increasing the level of insulin hormone in STZinduced diabetic rats.
Effect of periodic feed retrictions and refeeding on compensatory growth and blood physiology of of red tilapia (Oreochromis spp.) Adam Robisalmi; Kartiawati Alipin; Bambang Gunadi
Jurnal Iktiologi Indonesia Vol 21 No 1 (2021): February 2021
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v21i1.549

Abstract

Red tilapia is a one of the economically important fish species. In order to increase growth and feed efficiency, feed restriction strategies can be carried out. This study aims to determine the effect of periodic (weekly) restriction of feed on compensatory growth and physiological responses of red tilapia during the enlargement phase. The activity was carried out at the Fish Breeding Research Center for 4 months. The research method used a completely randomized design (CRD) 4 treatments with 3 replications, namely control (fish were fed daily), 1 (1 fasting day a week), 2 (2 fasting days a week), and 3 (3 fasting days a week). Fish rearing was conducted on the concrete tub measuring 2 x 1 x 0.8 m3 with a stocking density of 10 fish m-2. During the maintenance period of 120 days the fish were fed satiation twice a day. The results showed significant differences (P <0.05) in the value of weight growth, specific growth rate, daily growth and feed conversion ratio between control and other treatments, but not significantly different from treatment S1. The highest survival value was indicated by S1 treatment. The blood faal values of fish that were given feed restrictions consisting of red blood cells, white blood cells, hematocrit and hemoglobin showed that there was not significantly different than control fish (P> 0.05), which was at normal values. Restriction of feed to 1 day a week showed partial compensatory growth with better feed efficiency than fish fed daily. Abstrak Ikan nila merah merupakan salah satu jenis ikan yang memiliki nilai ekonomis tinggi. Peningkatan pertumbuhan dan efisiensi pakan dapat dilakukan dengan strategi pembatasan pakan. Penelitian ini bertujuan untuk menentukan pengaruh pembatasan dan pemberian pakan kembali secara berkala (setiap minggu) terhadap pertumbuhan kompensatori dan kondisi fisiologis ikan nila merah selama fase pembesaran. Kegiatan dilakukan di Balai Riset Pemuliaan Ikan selama 4 bulan. Metode penelitian menggunakan Rancangan Acak Lengkap 4 perlakuan dengan 3 kali ulangan yaitu kontrol (ikan diberi pakan setiap hari), 1 (1 hari puasa dalam seminggu), 2 (2 hari puasa dalam seminggu), dan 3 (3 hari puasa dalam seminggu). Pemeliharaan dilakukan pada bak beton ukuran 2 x 1 x 0,8 m3 dengan padat tebar 10 ekor m-2. Selama masa pemeliharan 120 hari ikan diberi pakan secara satiasi dua kali sehari. Hasil penelitian menunjukkan perbedaan nyata (P<0,05) pada nilai pertumbuhan bobot, laju pertumbuhan spesifik, pertumbuhan harian dan nisbah konversi pakan antara kontrol dengan perlakuan lainnya, namun tidak berbeda nyata dengan perlakuan 1. Adapun nilai sintasan tertinggi ditunjukkan perlakuan 1. Nilai faal darah ikan yang diberi pembatasan pakan terdiri sel darah merah, sel darah putih, hematokrit dan hemoglobin menunjukkan hasil yang tidak berbeda nyata dibandingkan ikan kontrol (P>0,05) yaitu berada pada nilai normal. Pembatasan pakan 1 hari dalam seminggu menunjukkan pertumbuhan kompensatori parsial dengan efisiensi pakan yang lebih baik dibanding ikan yang diberi pakan setiap hari.
Development of Mice Embryo (Mus musculus L.) after Closed Pulled Straw Vitrification in CZB Medium Muhammad Gunawan; Ekayanti Mulyawati Kaiin; Raden Cindy Rusherdiannita; Kartiawati Alipin
ANNALES BOGORIENSES Vol 23, No 2 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (952.946 KB) | DOI: 10.14203/ann.bogor.2019.v23.n2.72-80

Abstract

The objective of our present experiment was to investigate the effects of closed pulled straw (CPS) vitrification on the viability and development of mouse embryo. The experiment was arranged according to completely randomized design (CRD) consisting of 4 treatments, namely not vitrification (NV) is control, CPS 1 (mCZB Hepes + 20% Bovine Serum Albumin (BSA) +0.5 M sucrose + 10% EG + 10% DMSO), CPS 2 (mCZB Hepes + 20% BSA + 0.5 M sucrose + 15% EG + 15% DMSO), and CPS 3 (mCZB Hepes + 20% BSA +0.5 M sucrose + 20% EG + 20% DMSO) with 6 replications. The viability of embryos (%) was determined after 24 - 72 h of the culture period, while we also observed the percentage of embryos reaching the blastocyst stage (early blastocyst, expanded, hatching, and hatched). As a result, the treatments did not give a significant difference in the viability of embryos (P<0,05) but showed significant effects on embryo development (P<0,05). Furthermore, this present work conclusively found that CPS vitrification in CZB medium with cryoprotectants ethylene glycol (EG) and dimethyl sulfoxide (DMSO) noticeably influenced the development of mice embryo to reach the blastocyst stage, but showed no remarkable difference in the viability of embryo after culture for 24 – 72 h.
Efek Kombinasi Ekstrak Temulawak dan Belimbing Wuluh terhadap Perbaikan Histologis Ginjal Tikus yang Terpapar Herbisida Paraquat Kartiawati Alipin; Nining Ratningsih; Salsabila Nurjihan
BIOTIKA Jurnal Ilmiah Biologi Vol 20, No 2 (2022): BIOTIKA DESEMBER 2022
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v20i2.41713

Abstract

Paraquat merupakan herbisida yang digunakan pada pertanian. Akumulasi paraquat pada tanaman sayur jika dikonsumsi dapat bersifat racun dan menyebabkan gangguan pada organ ginjal seperti kerusakan pada glomerulus berupa edema spatium Bowman, serta pada tubulus berupa infiltrasi sel radang dan pembengkakan sel epitel tubulus. Tujuan penelitian ini yaitu untuk mengetahui pengaruh pemberian kombinasi ekstrak temulawak dan belimbing wuluh (TBW) terhadap perbaikan histologis ginjal tikus yang terpapar paraquat. Metode penelitian secara eksperimental menggunakan Rancangan Acak Lengkap dengan lima perlakuan dan lima ulangan. Induksi paraquat dilakukan pada seluruh perlakuan kecuali KN. Susunan perlakuan yang terdiri dari KN (tween 80 1%), KP (paraquat 25 mg/KgBB), P1 (kombinasi TBW 770 mg/KgBB), P2 (kombinasi TBW 385 mg/KgBB), P3 (kombinasi TBW 192,5 mg/KgBB). Parameter yang diamati yaitu histologis ginjal meliputi diameter glomerulus, lebar ruang Bowman, dan persentase nekrosis pada tubulus proksimal. Data dianalisis secara statistik menggunakan uji ANOVA pada taraf kepercayaan 95% kemudian dilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan terdapat peningkatan diameter glomerulus, penurunan lebar ruang Bowman serta penurunan persentase nekrosis sel tubulus proksimal pada perlakuan P1-P3 dengan dosis optimum yang dapat memperbaiki struktur histologis ginjal yaitu 385 mg/KgBB. Kesimpulan penelitian ini yaitu kombinasi ekstrak temulawak dan belimbing wuluh berpengaruh memperbaiki struktur histologis ginjal tikus yang terpapar paraquat.
EDUKASI PENGETAHUAN TENTANG PEMANFAATAN TUMBUHAN OBAT PADA MASYARAKAT DI BERBAGAI LOKASI KEDIAMAN MAHASISWA KKN INTEGRATIF UNPAD Kartiawati Alipin
Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Vol 11, No 4 (2022): Desember, 2022
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/dharmakarya.v11i4.38539

Abstract

Kekayaan Indonesia akan tumbuhan obat sangat besar. Pemanfaatannya sudah dilakukan sejak berabad yang lalu oleh nenek moyang sebagai ramuan tradisional dalam menyembuhkan berbagai penyakit maupun menjaga kesehatan. Namun seiring dengan perkembangan zaman pengetahuan tentang tumbuhan obat maupun  pemanfaatannya semakin menurun. Tujuan penelitian pada kegiatan PPM KKN Integratif Unpad periode Januari-Februari 2022 ini untuk memberikan edukasi pengetahuan tentang manfaat tumbuhan obat yang ada disekitar rumah. Metode yang digunakan yaitu melakukan wawancara terhadap responden mengenai pengetahuan tumbuhan obat, manfaatnya serta keterlibatannya dalam memanfaatkan TOGA, serta kegiatan penyuluhan daring melalui Webinar yang melibatkan mahasiswa KKN integrative Unpad periode Januari-Februari 2022 dan masyarakat di lokasi kediamannya. Hasil penelitian menunjukkan berdasarkan wawancara dalam satu RT dari 20 rumah diperkirakan hanya 20% yang menanam tumbuhan obat, hal ini menunjukkan terjadinya penurunan pengetahuan masyarakat akan manfaat dari tumbuhan obat. Setelah dilakukan edukasi melalui webinar maka didapat hasil pre-test dan post-test pada saat webinar terjadi peningkatan pengetahuan akan manfaat tumbuhan obat dari 75% menjadi 92%.Indonesia's wealth of medicinal plants is very large. Its use has been carried out since centuries ago by the ancestors as a traditional herb in curing various diseases and maintaining health. But along with the times, knowledge about medicinal plants and their use is decreasing. The purpose of the research in the Unpad Integrative KKN PPM activity for the January-February 2022 period is to provide education and knowledge about the benefits of medicinal plants around the house. The method used is conducting interviews with respondents regarding knowledge of medicinal plants, their benefits and involvement in utilizing TOGA, as well as online counseling activities through webinars involving Unpad integrative KKN students for the January-February 2022 period and the community in their residential locations. The results showed that based on interviews in one RT out of 20 houses, it was estimated that only 20% grew medicinal plants, this indicates a decline in public knowledge about the benefits of medicinal plants. After education through the webinar, the results of the pre-test and post-test during the webinar increased knowledge of the benefits of medicinal plants from 75% to 92%. 
Preferensi dan Variasi Pakan Echinodermata (Ophiocoma dentata dan Holothuria atra) terhadap Lamun dan Makroalga Tri Dewi Kusumaningrum Pribadi; Vessa Ramadhani; Kartiawati Alipin
BIOTIKA Jurnal Ilmiah Biologi Vol 20, No 2 (2022): BIOTIKA DESEMBER 2022
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v20i2.44974

Abstract

Echinodermata memiliki peran penting dalam ekosistem pesisir, khususnya ekosistem padang lamun karena membantu proses penguraian serasah pada kolom sedimen. Studi ini bertujuan untuk mendeskripsikan preferensi dan variasi pakan dari Echinodermata terhadap lamun dan makroalga, dengan menggunakan hewan uji dari spesies Ophiocoma dentata dan Holothuria atra. Bahan pakan yang disediakan adalah lamun (Thalassia hemprichii) dan makroalga (Padina australis, Sargassum sp., dan Ulva lactuca). Studi ini bersifat eksperimental berdurasi 72 jam, yang dilakukan pada suhu ruang dengan siklus pencahayaan alami. Pengamatan berat basah pakan sebelum dan sesudah eksperimen dilakukan dengan menimbang berat awal dan akhir pakan yang diberikan kepada hewan uji. Analisis isi perut dilakukan untuk melihat variasi pakan hewan uji dengan melakukan pembedahan organ pencernaan hewan uji. Nilai SDR menunjukkan bahwa pakan yang dominan dimakan oleh O. dentata dan H. atra adalah lamun (T. hemprichii). Studi mengenai preferensi dan variasi pakan memberikan informasi penting dalam menjaga siklus makan-memakan di ekosistem padang lamun sebagai salah satu upaya konservasi.
Sosialisasi Pemanfaatan Tanaman Herbal sebagai Obat Akternatif Penyakit Radang Sendi Desak Made Malini; Tia Setiawati; Kartiawati Alipin
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 6, No 4 (2023): Volume 6 No 4 April 2023
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v6i4.9682

Abstract

ABSTRAK Radang sendi atau Osteoartritis (OA) merupakan salah satu penyakit kronis yang paling sering terjadi pada orang dewasa. Penyakit ini termasuk penyakit degeneratif yang dapat menyebabkan nyeri, kekakuan dan kehilangan mobilitas pada sendi.  Pengobatan konvensional meliputi penggunaan obat antiinflamasi nonsteroid (NSAID) diketahui memiliki efek samping yang tidak diinginkan dan dapat mempengaruhi kualitas hidup pasien. Pengobatan dengan tanaman herbal telah menjadi alternatif yang menarik untuk pengobatan konvensional. Tujuan dilakukan kegiatan Pengabdian Pada Masyarakat (PPM) adalah meningkatkan pengetahuan masyarakat mengenai pemanfaatan tanaman herbal sebagai obat alterhatif OA sehingga dapat meningkatkan kualitas kesehatan masyarakat.  Kegiatan pengabdian yang dilakukan berupa penyuluhan dengan pre test dan post test, penyebaran kuisioner dan skriining kesehatan pada 40 orang wanita yang berumur 40-70 tahun. Berdasarkan hasil evaluasi pretest dan post test dari kegiatan ini terdapat peningkatan terkait pengetahuan responden tentang pemanfaatan tanaman herbal (100%), minat menggunakan tanaman herbal (97%) dan budidaya tanaman herbal (100%) obat alternative OA.  Sedangkan hasil skriining kesehatan menunjukkan bahwa nilai IMT (71%), tekanan darah (71%), kadar glukosa(55%), kolesterol (61%) dan Asam urat (40%) menunjukkan nilai rata-rata diatas normal. Dapat disimpulkan bahwa kegiatan PPM efektif untuk meningkatkan pengetahuan masyarakat  tentang pemanfaatan tanaman herbal sebagai obat OA dan masyarakat  juga menjadi lebih waspada akan kesehatannya. Kata Kunci: Sosialisasi, Radang Sendi, Tanaman Herbal  ABSTRACT Arthritis or Osteoarthritis (OA) is one of the most common chronic diseases in adults. This disease is a degenerative condition that can cause pain, stiffness, and loss of mobility in the  joints. Conventional treatment involves the use of nonsteroidal anti-inflammatory drugs (NSAIDs) which are known to have unwanted side effects and can affect the patient's quality of life. Treatment with herbal plants has become an attractive alternative to conventional treatment. The purpose of this Community Service activity is to increase public knowledge about the use of herbal plants as an alternative medicine for OA, thus improving the quality of public health. The community service activities carried out were in the form of counseling with pre-test and post-test, distribution of questionnaires, and health screening on 40 women aged 40-70 years. Based on the pre-test and post-test evaluation results of this activity, there was an increase in respondents' knowledge about the use of herbal plants (100%), interest in using herbal plants (97%), and herbal plant cultivation (100%) as an alternative medicine for OA. Meanwhile, the health screening results showed that the BMI (71%), blood pressure (71%), glucose levels (55%), cholesterol (61%), and uric acid (40%) values were above the normal average. It can be concluded that the community service activity was effective in increasing public knowledge about the use of herbal plants as an alternative medicine for OA, and the public also became more aware of their health. Keywords: Socialization, Arthritis, Herbs   
Effect of Different Feed Restrictions on Growth, Biometric, and Hematological Response of Juvenile Red Tilapia (Oreochromis spp) Adam Robisalmi; Kartiawati Alipin; Bambang Gunadi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 13 No. 2 (2021): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v13i2.26302

Abstract

Highlight ResearchThere has been a significant decrease in growth, condition factors, hepatosomatic index along viscerosomatic index with the length of time for feed restriction, although fasting for up to 28 days did not cause fish mortalityHematological parameters such as RBCs, WBCs, hematocrit, and hemoglobin decreased significantly, but after re-feeding increased significantly in consecutive timesThe blood glucose levels decreased during feed restriction and gradually increased after re-feedingFeed restriction fish gained weight continuously during re-feeding, but no displaying compensatory growthThe decrease in hematological parameters did not indicate stress levels in fish, but reflected a lack of nutrition condition AbstractThe management of red tilapia culture can be improved through restriction of feed. This study aimed to determine the impact of feed restriction time and re-feeding on the growth and hematological performance of juvenile red tilapia. This study used an experimental method with a completely randomized design consisting of five treatments with four replications, namely the fish were given food for 28 days (A), 7 days of feed restriction followed by 21 days of refeeding (B), 14 days of feed restriction followed by 14 days of refeeding (C), 21 days of feed restriction followed by 7 days of refeeding (D) and 28 days of feed restriction (E). The parameters observed were growth, biometry, and hematological values. The results showed that during the feed restriction period there was a significant decrease in growth, condition factors, hepatosomatic index along with viscerosomatic index with the length of time for feed restriction, although fasting for up to 28 days did not cause fish mortality. Hematological parameters such as RBCs, WBCs, hematocrit, and hemoglobin decreased significantly, but after re-feeding, they increased significantly in consecutive times. As for the blood glucose levels decreased during feed restriction and gradually increased after re-feeding. This study stated that feed restriction fish gained weight continuously during re-feeding, but no displaying compensatory growth. The results suggested that the decrease in hematological parameters did not indicate stress levels in fish, but reflected a lack of nutrition condition.