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All Journal International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Althea Medical Journal Indonesian Journal of Biotechnology Global Medical and Health Communication The Indonesian Biomedical Journal Jurnal Sistem Kesehatan Universa Medicina Paediatrica Indonesiana GIZI INDONESIA Chimica et Natura Acta Indonesian Journal of Chemistry Journal of Epidemiology and Public Health The Indonesian Journal of Infectious Diseases FLUIDA Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Tunas Medika Jurnal Kedokteran & Kesehatan The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Jurnal Penyakit Dalam Indonesia
Bachti Alisjahbana
Infectious Diseases And Tropical Diseases Division, Department Of Internal Medicine, Faculty Of Medicine Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : Indonesian Journal of Biotechnology

 1 
Early development of self‐administered COVID‐19 rapid test based on nucleocapsid detection in saliva sample Siti Soidah; Toto Subroto; Sari Syahruni; Fauzian Giansyah; Henry Chandra; Dhiya Salsabila; Bachti Alisjahbana; Nisa Fauziah; Hesti Lina Wiraswati; Leonardus Wiydatmoko; Basti Andriyoko; Anita Yuwita; Muhammad Yusuf
Indonesian Journal of Biotechnology Vol 27, No 3 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.72269

Abstract

More than 6,000,000 people have died due to the coronavirus (COVID‐19) pandemic. This disease spread quickly due to its highly contagious nature. The SARS‐CoV‐2 virus that causes the disease can be transmitted through saliva droplets secreted by infected people at a distance of less than 1 m. As a result, saliva has been accepted as an alternative specimen for COVID‐19 detection by the Centers for Disease Control and Prevention (CDC). Furthermore, WHO recommended the use of rapid antigen tests based on lateral flow immunoassay when reverse transcription‐polymerase chain reaction (RT‐PCR) is not available. We developed a saliva‐based rapid antigen test by optimizing the antibody concentration and optimum pH for the conjugation of antibody and gold nanoparticles. We found that the best running buffer formulation consisted of 75 mM sodium phosphate buffer, 1% NaCl, 1% Triton X‐100, 0.5% N‐acetyl‐L‐cysteine, and 0.02% sodium azide. The addition of a mucolytic agent in the buffer can reduce the viscosity of saliva, thus improving sensitivity. The rapid test developed detected the lowest concentration of nucleocapsid protein at 0.1 μg/mL. Our study revealed 100% specificity against negative COVID‐19 saliva and no cross‐reaction with avian influenza virus hemagglutinin.
Co-Authors Afandi, Ressa Novita Agnes Rengga Indrati Ahmad Sulaeman Amaylia Oehadian Andre van der Venn Angèle JGMGerver-Jansen Angky Tririni Anita Yuwita Anna Alisjahbana Annisa Ikhsanawati, Annisa Ardini S. Raksanagara Ardini S. Raksanagara, Ardini S. Baskoro, Toni Basti Andriyoko Bestari, Muhamad Begawan Bevi Lidya Bevi Lidya Budi Santoso Budiman , Budiman Clara M Kusharto Depamede, Brian Umbu Rezi Dewi Kartika Turbawaty Dhiya Salsabila Dick van Soolingen Dida Ahmad Gurnida Dwi Agustian Dwihardiani, Bintari Edhyana Sahiratmadja Endang Sutedja Eva Mardiana Hidayat Evan Susandi Fauzian Giansyah Febriani, Ulfah Dwi Felisia . Finny Fitry Yani Gumilang, Manik Intan Guntur Darmawan Hadyana Sukandar Hafidhoh, Sanny Hendra Subroto, Hendra Henriette A Delemarre–van de Waal Henry Chandra Herry Garna Hesti Lina Wiraswati Hidayat, Eva Mardiana Hikmat Permana Hikmat Permana Hinta Meijerink Hofiyah Djauhari Ida Parwati Ida Parwati Iman Rahayu Irvan Afriandi Jeffery Malachi Candra Jose RL Batubara Juhariah Juhariah Julia Hartati Koesoemadinata, Raspati C. Korry Novitriani Krishna Yana Krisnian, Tharani Kumia Wahyudi Kusumawardhani, R.N. Yasmin Lelly Yuniarti Leonardus Wiydatmoko Loe, Luse marni banowati Menik Herdwiyanti Muhamad Zulchoir Rasyid Muhammad Yusuf Muhammad Yusuf Muhammad Yusuf Nanan Sekarwana Nanny Natalia Mulyani Soetedjo Nanny Natalia Mulyani Soetedjo Ni Sayu Dewi B. Nina Dwi Putri Nisa Fauziah Novi Jayanti Nuni Sulastri Nurizzatun Nafsi Nurul Hidayah Chairunnisa Nururrohmah, Siti Nury Fitria Dewi obin sarwita Panji Fortuna Hadisoemarto Parse, Rocci Jack Prayudi Santoso Putri Vidyaniati Ratna Yulistya Ningrum Reinout van Crevel Reinout van Crevel Ressa Novita Afandi Resvi Livia Rina Triasih Rocci Jack Parse Rovina Ruslami Rovina Ruslami, Rovina Santoso, Ida Parwati Sari Syahruni Setyaningrum, Sinta Siti Soidah Sofia Imaculata Stephen M. Graham, Stephen M. Sulaiman Ngongu Depamede Suparman Hardinsyah, Suparman Susan Margaret McAllister Susandi, Evan Susantina Prodjosoewojo Syndi Nurmawati Teow Sheng Hao Tharani Krisnian Tjahjani M. Sudiro Tony Sadjimin Toto Subroto Toto Subroto Toto Subroto Trinugroho Heri Fadjari, Trinugroho Heri Trisasi Lestari, Trisasi Tryawan, Ilham Ngongu Tya Listiaty Ulfah Dwi Febriani Umi Baroroh Uun Sumardi, Uun Willem J Gerver Wirawan, Chevie Woro Indri Padmosiwi Yana, Krishna Yani Triyani Yani Triyani Yanti Mulyana Yeva Rosana Yovita Hartantri Yulniar Tasli