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INHIBITORY EFFECT OF Sansevieria trifasciata L ON AERIAL PATHOGENIC MICROFUNGI IN TUTORIAL ROOMS Andayani, Sharah Julia; Amanah, Amanah; Permatasari, Tissa Octavira
Proceedings of the International Conference on Applied Science and Health No 3 (2018)
Publisher : Proceedings of the International Conference on Applied Science and Health

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Abstract

Background: Air pollutants in a room can be caused by several things, such as microorganisms in the form of fungi. Fungi that dispersed in air with concentration>700 CFU/m3 can be categorized as air pollution which could lead to many symptoms of various human diseases An effort that may improve indoor air pollution is using anti pollutant plant such as Sansevieria trifasciata L. This study aims to determine the effectiveness of Sansevieria trifasciata L on the concentration of aerial pathogenic microfungi in the tutorial room in Faculty of Medicine Swadaya Gunung Jati University and identify the aerial pathogenic microfungi species in the tutorial room. Methods: This study was a quasi experimental research with pre and post-test group design. Eight tutorial rooms with 4 repetitions were tested for species microfungi growth using Saburoud Dextrose Agar (SDA) media in 32 petri discs. After 7 days of incubation, microfungi were identified and the colony form unit (CFU) number was counted. The data was analyzed using paired T test. Results: Ten aerial pathogenic microfungi growth were significantly (p = 0.000) inhibited by Sansevieria trifasciata L demonstrated by CFU number reduction from 54.18 – 204.94 CFU/m3 to 16.48 – 44.75 CFU/m3. Conclusions: Sansevieria trifasciata L effectively inhibited aerial pathogenic microfungi growth in tutorial rooms. 
COMPARISON OF EFFECTIVENESS BETWEEN CELERY JUICE (Apium graveolens L.) AND 2% MICONAZOLE TOWARDS THE GROWTH OF Malassezia furfur Hammada, Ronaa; Pratiwi, Witri; Fauzah, Shofa Nur; Nauphar, Donny; Amanah, Amanah
Proceedings of the International Conference on Applied Science and Health No 3 (2018)
Publisher : Proceedings of the International Conference on Applied Science and Health

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Abstract

Introduction: Pityriasis versicolor is caused by the fungi Malassezia furfur with a worldwide prevalence of 50%, including tropical countries, second only to dermatitis in Indonesia. Pityriasis versicolor is difficult to treat and requires long-term treatment. The disease has high recurrence risk and may cause drug resistance. 2% Miconazole is known to have long-term side effects; therefore, alternative treatment is needed. Several studies suggested that celery (Apium graveolens L.) contains active substances with anti-fungal properties. This paper aims to investigate the comparison of effectiveness between celery juice and 2% Miconazole towards the growth of Malassezia furfur. Methods: This is an in-vitro experimental study with post-test only control group design. The subjects were split into 5 groups which were given celery juice in 10% DMSO with the concentration of 10%, 20%, 30%, 40%, and 50%. A negative control group was given only 10% DMSO and the positive control group was given 2% Miconazole. The data were then analyzed using Kruskall-Wallis followed by Mann-Whitney test. Results: The Kruskall-Wallis test showed all concentration of celery juice had antifungal effect with p=0.000 (p<0.05) and were effective in inhibiting the growth of Malassezia furfur. The Mann-Whitney test showed that the 50% celery concentration was as effective as 2% Miconazole in inhibiting the growth of Malassezia furfur (p=0.495). Conclusion: Celery juice (Apium graveolens L.) was effective in inhibiting the growth of Malassezia furfur with 50% concentration as the most effective concentration. 
POTENTIAL ACCELERATING EFFECT OF Ageratum conyzoides L. LEAVES EXTRACT ON FIBROBLASTS DENSITY OF INCISION WOUND OF MALE WHITE MICE (Mus musculus) Lestari, Mega Ayu; Sari, Ariestya Indah Permata; Amanah, Amanah
Proceedings of the International Conference on Applied Science and Health No 3 (2018)
Publisher : Proceedings of the International Conference on Applied Science and Health

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Abstract

Background: Wound treatment using traditional medicine has been known widely in various countries in the world. Ageratum conyzoidesL. is commonly known by the ancient people to treat wound due to its potential anti-inflammatory effect. This study aims determine the effect of Ageratum conyzoidesL. leaves extract on fibroblast density of incision wound of male white mice (Mus musculus). Methods: This post-test only control group design experimental study used 35 male white mice which were randomly divided into five groups, i.e. negative control group K(-), positive control group K(+) (10% povidone iodine), and treatment group P1, P2, and P3 that were each given billy-goat weed leaf with increasing dose (15%, 30%, and 45% respectively). On each day, the length of the incision was measured by a ruler. After 7 days, the mice were terminated to obtain wound tissue which were used to prepare H&E stained histopathological sections to observe fibroblast density. Non-parametric analyses using Kruskall-Wallis and Mann Whitney test were used to compare the wound length and fibroblasts density. Results: Lengths of incision wound between all pairs of groups at the 7th day are significantly different (p< 0.05) with group P3 showed the shortest one. Significant differences were also observed in fibroblasts density between group K(-) and K(+), K(-) and P1, K(-) and P2, K(-) and P3, K(+) and P3, P1 and P3, P2 and P3 (p< 0.05) with group P3 showed the highest density among all groups. Conclusions: Ageratum conyzoidesL. leaves extract 45% has more potential effect than povidone iodine 10% in accelerating healing process by enhancing fibroblasts density. 
APLIKASI BIJI KEFIR AIR SEBAGAI BAHAN PENGEMBANG ROTI KUKUS Pertiwi, Sri Rejeki Retna; Novidahlia, Noli; Amanah, Amanah
JURNAL PERTANIAN Vol 8, No 2 (2017): OCTOBER
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.068 KB) | DOI: 10.30997/jp.v8i2.1053

Abstract

Biji kefir air berupa matrik gel polisakarida, bening seperti kristal, didalamnya terdapat simbiosis kompleks antara bakteri asam laktat dan khamir.  Penelitian ini bertujuan mendapatkan konsentrasi optimal biji kefir air sebagai pengembang roti kukus.   Roti kukus dibuat dengan tiga konsentrasi biji kefir air: 75, 100, dan 125% berdasarkan 100% tepung, kemudian dianalisis sifat mutu fisik dan sensorinya.  Roti kukus dengan pengembang biji kefir air terbaik dibandingkan sifat fisik dan kadar seratnya dengan yang diolah menggunakan khamir (standar).  Hasil penelitian menunjukkan bahwa roti kukus yang dibuat dengan biji kefir air 100% memiliki mutu fisik (volume spesifik, struktur remah) lebih baik daripada yang diproses dengan biji kefir air 75% dan 125%.  Mutu sensori roti kukus yang dibuat dengan 100% dan 125% biji kefir air (kenampakan, kehalusan permukaan, pori, tekstur, kelengketan, aroma) lebih tinggi dibandingkan yang diolah dengan biji kefir air 75%.  Penggunaan biji kefir air 100% ditentukan sebagai konsentrasi bahan pengembang optimal pada pembuatan roti kukus.  Roti kukus yang dibuat dengan biji kefir air 100% memiliki volume spesifik dan struktur remah tidak beda nyata dengan roti kukus standar, sedangkan kandungan serat pangannya lebih tinggi.  Kandungan serat pangan berturut-turut roti kukus dengan biji kefir 100% adalah  18.5% (bk) dan roti kukus standar 12.5% (bk).  Dapat disimpulkan bahwa konsentrasi biji kefir air optimal sebagai bahan pengembang roti kukus adalah 100% berdasarkan 100% tepung.Kata kunci: bakteri asam laktat (BAL), biji kefir air, khamir, roti kukus. APLICATION OF WATER KEFIR GRAINS AS LEAVENING AGENT OF STEAMED BREAD ABSTRACTWater kefir grain is a complex symbiosis between lactic acid bacteria and yeast in transparent gel matrix crystal-like.  The objective of this study was to get optimum concentration of water kefir grains as leavening agent of steamed bread.  Steamed bread was prepared with three concentrations of water kefir grains: 75, 100, and 125% based on 100% flour, then their physical and sensory properties were analyzed.  The chosen steamed bread using water kefir grains was then compared its physical properties and fiber content to the standard steamed bread using yeast.  Results showed that steamed bread made with 100% of water kefir grains had better physical properties (specific volume, crumb structure) than that of 75% and 125%.  The sensory properties (appearance, surface smoothness, crumb structure, tenderness, adhesiveness, aroma) of steamed bread made with 100% and 125% of water kefir grains were superior than that of 75%. The use of 100% water kefir was chosen as the optimum concentration for leavening agent of steamed bread.  Steamed bread using 100% water kefir had no differences on specific volume and crumb structure compared to those of standard steamed bread, whereas its fiber content was significantly higher.  Fiber content of steamed bread using 100% water kefir grains and steamed bread using yeast was 18.51% (db) and 12.51% (db), respectively.  It can be concluded that optimum concentration of water kefir grains used as leavening agent for steamed bread making was 100% based on 100% of flour.
Obama Ciput (Community Based Management of Krucil’s Puppets Entrepreneur) Strategi Melestarikan Kesenian Wayang Krucil melalui Program One Village One Product di Kabupaten Blora Setyowati, Fitri Andriani; Amanah, Amanah; Warmih, Warmih; Oktarina, Nina
Jurnal Abdimas Vol 23, No 1 (2019): June
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LP2M), Universitas Negeri Semarang

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Abstract

Indonesia memiliki potensi kesenian yang luar biasa salah satunya kesenian wayang krucil. wayang krucil merupakan jenis wayang yang terbuat dari kombinasi antara kayu dan kulit sapi yang berasal dari Kabupaten Blora, memiliki ukuran lebih kecil dari wayang golek dan wayang kulit. Namun sampai saat ini wayang krucil tidak terkenal sebagaimana wayang golek dan wayang kulit Cirebon serta keberadaan wayang krucil berada didalam 75 jenis wayang yang hampir punah di Indonesia. Hal ini disebabkan karena jumlah pembeli wayang krucil semakin menurun sehingga banyak pengusaha wayang krucil tidak aktif lagi menjalankan usahanya. Selain itu disebabkan karena generasi muda telah mengenal kesenian dan budaya dari luar dan mulai meninggalkan kesenian tradisional, hal ini membuktikan bahwa perlu adanya tindakan serius dari pemuda khususnya dari pemerintah melakukan upaya untuk tetap melestarikan kesenian yang membuat Kabupaten Blora  tetap terkenal sebagai kabupaten yang memiliki wayang uniknya. Oleh karena itu penulis mengajukan gagasan “OBAMA CIPUT” (Community Based Management of Krucil’s Puppets Entrepreneur): Strategi Melestarikan Kesenian Wayang Krucil Melalui Program One Village One Product di Kabupaten Blora. Community Based Management of Krucil’s Puppets Entrepreneur merupakan pengelolaan berbasis masyarakat yang dilakukan oleh para pengusaha wayang krucil dimana masyarakat lokal terlibat secara aktif dalam proses pelestarian dan pengelolaan kesenian wyang krucil yang bertempat di Desa Klopodhuwur. Dengan adanya Community Based Management of Krucil’s Puppets Entrepreneur diharapkan kesenian wayang krucil akan tetap terjaga karena setiap minggunya masyarakat Desa Klopodhuwur akan dilatih untuk membuat wayang krucil dengan bentuk dan corak yang beranekaragam, dalam hal ini nilai guna wayang krucil tidak hanya sebagai media hiburan tetapi juga dapat digunakan sebagai inverior rumah tangga serta media pembelajaran yang menarik. Desa Klopodhuwur merupakan sebuah desa yang memiliki kelompok Suku Samin, keberadaan Suku Samin membuat daya tarik wisatawan untuk datang dan melihat kehidupan Suku Samin. Adanya wisatawan yang berkunjung di Desa Klopodhuwur sebagai salah satu strategi pemasaran wayang krucil karena para wisatawan yang ingin membeli oleh-oleh wayang krucil dapat langsung membeli di pusat penjualan wayang krucil di Desa Klopodhuwur. Hal ini akan menarik minat wisatawan untuk berkunjung lebih lama di Kabupaten Blora. Dengan strategi tersebut, akan berdampak kepada tempat-tempat wisata lain yang ada di Blora untuk dikunjungi para wisatawan sehingga akan meningkatkan perekonomian Kabupaten Blora dan akhirnya akan terwujud Indonesia berdaulat
Increasing Legal Capacity for Communities in the Context of Realizing a Village of Law Awareness and Child Friendly Prayogo, Bagus Edi; Amanah, Amanah; Pradana, Tirta Mulya Wira; Rodiyah, Rodiyah
Indonesian Journal of Advocacy and Legal Services Vol 1 No 1 (2019): Strengthening Community and Legal Sector in Indonesia
Publisher : Faculty of Law, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/ijals.v1i1.33776

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The purposes of community services program and research are to support, analyze, and create an accountable, transparent, independent, legally-aware and child-friendly village in Kedungkelor village, Warureja sub-district, Tegal regency. The paper analyzed two major parts are taken in the title of the service as well as aware of the law and child worth. Legal awareness and worthy of children are considered important because the child's world is very determining how the child will grow up later so that the village apparatus and the community need to increase of awareness related to the law and in the policies formulated by the village government or the community itself must be child-oriented. The program implemented a to realize a village that is aware of the law and suitable for children in our service is providing free legal assistance, door-to-door programs for families aware of the law and the environment, leadership training, healthy snacks and hand hygiene programs, and anti-bullying programs. The paper concluded that there are some forms of activity that will initiate the formation of a village that is aware of the law and is suitable for children.
CALCULATING COLONY OF Candida spp. AMONG CHILDREN WITH INTELLECTUAL DISABILITY IN CIREBON Oktaviani, Dhini; Amanah, Amanah; Suhaeni, Eni; Primanagara, Risnandya
Proceedings of the International Conference on Applied Science and Health No 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Background: Children and adults with Intellectual Disability and Autism Spectrum Disorders (ASD) have decreased intestinal permeability and enzyme activity, causing gastrointestinal (GI) problems that are more frequent and more severe than in children from the general population. The immune system which is part of the intestinal barrier in children with Intellectual Disability (ID) experiences various disorders and becomes more susceptible to fungal invasion, such as Candida spp. This present study aims to calculate the colony of Candida spp. in the digestive tract of Children with Intellectual Disability (ID) in the city of Cirebon.Method: This is an observational study with cross-sectional design using purposive sampling. The study was conducted in November 2018-February 2019. The sample (ID) group consists of 31 individuals from Special Needs School and 60 individuals from National elementary school (non-ID) in the city of Cirebon. The inclusion criteria were children ages 6-12 years and the exclusion criteria were children under antifungal treatment and children under cytotoxic or immunosuppressive drugs.  The fecal samples from the ID group and non-ID were examined at the Laboratory of the Faculty of Medicine, Universitas Swadaya Gunung Jati Cirebon, Indonesia using Digital Colony counter. Collected data were stored and analyzed using computer aided Statistical program. Mann-Whitney analysis of variance techniques were used to test the hypotheses. Results: The results showed that there are significant differences in the colonies of Candida spp. between the ID group and non-ID group. Median colony of Candida spp. in the ID group is 39.00 with mean colony of 126.90 while the median colony of non-ID group is 3.00 with mean colony of 40.93 (p<0.001).Conclusion: There are significant differences between the Candida spp. colonies in the ID group with the non-ID.
INHIBITORY TEST OF DAYAK ONION (ELEUTHERINE BULBOSA MILL.) ESSENTIAL OIL TO THE GROWTH OF MALASSEZIA FURFUR Hayati, Syahidatun; Amanah, Amanah; Indriyati, Rose
Proceedings of the International Conference on Applied Science and Health No 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Abstract

Background: Certain fungi are normal flora on human mucosa and skin, and they require lipids for growth. One of them is Malassezia furfur, which can develop into an opportunistic infection and will take a long time to treat. The fungus may cause pityriasis versicolor, which cause scaly and discoloration of the skin. It is estimated that 40-50% from population in the tropical country is infected. Dayak onion (Eleutherine bulbosa Mill.) essential oil have the potential to treat fungal infections. This research aims to see the inhibitory potential of dayak onion essential oil (Eleutherine bulbosa Mill.) in inhibiting the growth of Malassezia furfur.Methods: This is an experimental study with a post-test control group design. The samples grouped into 7 groups. The first five treatment (P1 to P5) was given dayak onion (Eleutherine bulbosa Mill.) essential oil with a concentration of 3.125%,6.25%, 12.5%, 25%, and 50%, P6 (positive control) was given ketoconazole, and P7 (negative control) was given 10% DMSO. The inhibitory effect is measured from the diameter of the inhibitory zone, then analyzed using Kruskal-Wallis and Mann-Whitney tests.Result: The result showed that average diameter on 50% concentration is 9.25mm, 25% concentration is 7.5mm, 12.5% concentration have diameter of 4.5mm, 6.25% concentration have diameter of 3mm, 3.125% concentration with diameter of 1.75mm, positive control group with diameter of 20mm, and negative control group with diameter 0mm.Conclusions: Essential oil of dayak onion (Eleutherine bulbosa Mill.) shows inhibition of the growth of Malassezia furfur. The smallest inhibition zone diameter was 1.75 mm with concentration of 3.125%. The largest inhibition zone at a concentration of 50% with diameter of 9.25 mm. This finding showed potential effects towards antifungi treatment.
COMPARISON OF SOIL-TRANSMITTED HELMINTH EGG INFESTATION IN FECES OF INTELLECTUAL DISABILITY (ID) CHILDREN WITH NON-ID CHILDREN Sujana, Mellyna Iriyanti; Amanah, Amanah; Handoyo, Moch Yusuf
Proceedings of the International Conference on Applied Science and Health No 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Abstract

Background: The worm infestation is considered as one of the causes of cognitive function disruption. It also can aggravate the condition in children with intellectual disability (ID). This greatly affects health conditions, such as abdominal pain, bloating, diarrhoea, constipation and other digestive disorders. Indigestion may increase aggressive behaviour, mood change and malnutrition so that it leads to chronic malnutrition which is the cause of major morbidity and premature death in the ID population. This study aimed to compare soil-transmitted helminth egg infestation in stool samples between ID children and non-ID children.Methodology: This present study was a descriptive study with a cross sectional design. Stool samples was collected from 30 students from Special Need School  and 60 students from Elementary School in the Cirebon City. The flotation method was used to identify the egg of soil-transmitted helminth. The stool samples were examined in the Laboratory of the Faculty of Medicine, Universitas Swadaya Gunung Jati, Cirebon, IndonesiaResults: The laboratory test found positively Soil-transmitted Helminth eggs in 9 of 30 stool sample of  ID students. Among them, it was found 5 stool samples with Ascaris lumbricoides, 2 stool samples of Trichuris trichiura, and 2 stool samples of Necator americanus. Regarding their levels, 4 students (44.4%) had mild intellectual disability and 5 students (55.6%) had moderate intellectual disability. However, there was not found any Soil-transmitted Helminth eggs in stool samples of non-ID children. Conclusion: Soil-transmitted helminth eggs was found only in stool samples of ID children. The hygiene and sanitation in the school should be guaranteed to prevent the transmission of Soil-transmitted Helminth.
In Vitro Inhibitory Activity of Dayak Onion (Eleutherine bulbosa Mill.) Essential Oil Against Klebsiella pneumoniae Hermawan, Marwan; Amanah, Amanah; Nurbaiti, Nurbaiti
GHMJ (Global Health Management Journal) Vol 3, No 3 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.204 KB) | DOI: 10.35898/ghmj-33458

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Background: The cases of Klebsiella pneumoniae infec on in West Java province is s ll high. Klebsiella pneu- moniae bacterium can produce the Extended Spectrum Beta Lactamase enzyme leading to an bio cs resistant, so it is important to look for natural and inexpensive an bio c alterna ves; one of which is Dayak onion plant (Eleutherine bulbosa Mill). The content found in Dayak onion is believed to inhibit the growth of Klebsiella pneu- moniae bacteria. This study aims to determine the effec veness of Dayak onion essen al oil on the growth of Klebsiella pneumoniae bacteria. Methods: This study was an experimental study with a post-test control group design using the culture of Kleb- siella pneumoniae on the media Muller Hinton Agar in 7 groups. Groups 1 to 5 (K1 to K5) were given sequen ally Dayak Onion essen al oil solu on in 10% DMSO solvent with concentra ons of 50%, 25%, 12.5%, 6.25%, and 3.125%. Group 6 (K6) was posi ve control (+) with 5 ?g Levofloxacin, and Group 7 (K7) was Nega ve (-) with 10% DMSO. The treatment results were measured by the diameter of the host zone and the collected data were analyzed using One-way ANOVA with appropriate Post hoc analysis. Results: The results showed that that there are differences in each group to inhibit the growth of bacteria (p <0.005). K1 are higher than K2, K3, K4, K5, and K7 with increasing concentra ons showed greater inhibi on. Levofloxacin (K6) showed the highest inhibitory power. Conclusion: The results showed that that there are differences in each group to inhibit the growth of bacteria (p <0.005). K1 are higher than K2, K3, K4, K5, and K7 with increasing concentra ons showed greater inhibi on. Levofloxacin (K6) showed the highest inhibitory power.