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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK Innofarm : Jurnal Inovasi Pertanian Agrointek Indonesian Journal of Cancer Chemoprevention Jurnal Riset Kimia KOVALEN: Jurnal Riset Kimia Al-Kimia Indonesian Journal of Pure and Applied Chemistry CHEESA: Chemical Engineering Research Articles Jurnal Teknologi Kimia Unimal Journal of Fisheries & Marine al Kimiya : Jurnal Ilmu Kimia dan Terapan
Puji Ardiningsih
Department of Chemistry, Mathematics and Natural Sciences Faculty, Tanjungpura University, Jl. A. Yani, Pontianak, Kalimantan Barat, Indonesia.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Earth & Planetary Sciences Education Engineering Environmental Science Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Physics

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Journal : Al-Kimia

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Secondary Metabolites and Antioxidant Activity of Methanol Extract and Fractions from the Root of Gitaan (Willughbeia coreacea Wall) Rudiyansyah; Edwindar, Felgi; Ardiningsih, Puji
Al-Kimia Vol 11 No 2 (2023): DESEMBER
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

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Abstract

Gitaan (Willughbeia coreaceae) is one of Indonesian endemic plants and belongs to Apocynaceae family that is potential for antioxidant source. This family contains triterpenoids, phenolics, alkaloids, and cardenolides, unfortunately information about secondary metabolites together with its biological activities from W. coreaceae is scarce. This study was conducted to determine secondary metabolites and antioxidant activity from the methanol extract and fractions of gitaan root. The root powder was macerated, its secondary metabolites were analyzed by phytochemical test and the antioxidant activity was evaluated by the qualitative and quantitative DPPH (1,1-diphenyl-2-picrylhydrazil) methods with the ascorbic acid was used as a positive control. Results showed the methanol extract and methanol fraction have alkaloids, polyphenols, and terpenoids, and ethyl acetate and n-hexane fractions contained alkaloids and polyphenols. By DPPH qualitative, all samples except for n-hexane fraction showed antioxidant properties which were indicated by yellow stains on the thin layer chromatography (TLC) plate after sprayed with 50 ppm of DPPH. By quantitative, methanol extract and fractions of methanol, ethyl acetate, n-hexane, and the ascorbic acid showed antioxidant activity with IC50 values of 44.1181 ppm, 45.4060 ppm, 57.2484 ppm, 983.9576 ppm, and 0.0445 ppm, respectively. Subsequently, methanol extract has better antioxidant activity then other fractions.
Co-Authors Adhitiyawarman, Adhitiyawarman Agus Wibowo, Muhammad Ajuk Sapar Alatin, Isam Andi Hairil Alimuddin Aprenesya, Jamarlia Ari Widiyantoro Aritonang, Anthoni Batahan Ashari, Asri Mulya Edwindar, Felgi Firdaus, Giska Ristianty Hasanah, Kutsiatul Hendra Perdana Imelda Hotmarisi Silalahi Jayuska, Afghani Juniarti, Leni Mega Sari Juane Sofiana Millenia, Millenia NOPIANTI, NOPIANTI Nurul Huda Pitri, Sela Rahma Rani, Diah Puspa Rendi, R. Rio Kurniadi Risa Nofiani Roeswandi, Irine Fajrin Rudiyansyah Rudiyansyah Rudiyansyah Sadenta, Deby Pascalia Sari, Heni Puspita Siti Khotimah Sri Wahyuni Sulistyo, Luthfi Imam Suparnawati, Suparnawati Triswanto, Bambang Warsida, W. Warsidah, Warsidah Wibowo, Muhamad Agus Widodo, Muhammad Agus Yuniar, Risa