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All Journal Jurnal Kedokteran Brawijaya Prosiding UNEJ e-Proceeding BIOTROPIC The Journal of Tropical Biology Acta Biochimica Indonesiana Molekul: Jurnal Ilmiah Kimia Scripta Biologica Jurnal Manusia dan Lingkungan
Saefuddin Aziz
Fakultas Biologi, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Medicine & Pharmacology Social Sciences

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Journal : UNEJ e-Proceeding

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HIGHLY SPESIFIC BACILLUS CEREUS-PHAGES ISOLATED FROM HOSPITAL WASTEWATER IN BANYUMAS REGENCY Anwar Rovik; Saefuddin ‘Aziz; Hendro Pramono
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Bacillus cereus is pathogenic bacteria that frequently contaminate food by producing entero and emetic toxins. B. cereus had shown resistance to various antibiotics. An alternative to control B. cereus is the use of bacteriophages. This study aims to determine whether bacteriophages isolated from hospital wastewater in Banyumas Regency is highly specific to B. cereus. The research was carried out descriptively through isolation, purification, enumeration, specificity, effectivity, and host-lysing rate test. B. cereus phages were isolated from hospital wastewater in Banyumas Regency with various titer, i.e. 108-109 PFU.ml-1. The PKRW-1, PKCL-1, PKSR-2, RSBMS-2, RSBMT-1, RSAJP-1, RSAJP-2, RSAJK-2, RSAJE-1, and RSAJE-3 phages have a great specificity and effectivity of infection to B. cereus. Total of 7 isolated phages have a fast host-lysing rate by the 2nd hour of incubation.
Co-Authors . HERNAYANTI Adi Amurwanto Afifah Mariana Agung Saprasetya Dwi Laksana, Agung Saprasetya Dwi AGUS HERY SUSANTO Agus Sabdono Alfi Muntafiah Alice Yuniaty Anwar Rovik Budi Rustomo Daniel Joko Wahyono Dini Ryandini Dwi Priyanto Dwiana Muflihah Yulianti Dyah Fitri Kusharyati Fitranto Arjadi Hendro Pramono Hendro Pramono Hendro Pramono Hernayanti Hernayanti Meyta Pratiwi Nurtjahjo Dwi Sasongko Ocky Karna Radjasa Oedjijono Ratna Stia Dewi Riyanti Rizal Khoirun Alfisah Sari, Regita Cahaya Shoffiana Noor Sri Martina Wiraswati Taruna Dwi Satwika Tri Ramadhani Yuriza Eshananda