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All Journal Jurnal Teknologi Dan Industri Pangan Bionatura Dentika Dental Journal The Indonesian Journal of Dental Research Dental Journal (Majalah Kedokteran Gigi) Scientific Dental Journal Journal of Functional Food and Nutraceutical Jurnal of Syiah Kuala Dentistry Society
Boy M Bachtiar
Department Of Oral Biology, Faculty Of Dentistry, Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing

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The ability of IgY to recognize surface proteins of Streptococcus mutans Basri A. Gani; Santi Chismirina; Zinatul Hayati; Endang Winiati B; Boy M. Bachtiar; I. Wayan T. Wibawan
Dental Journal (Majalah Kedokteran Gigi) Vol. 42 No. 4 (2009): December 2009
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.77 KB) | DOI: 10.20473/j.djmkg.v42.i4.p189-193

Abstract

Background: Streptococcus mutans are gram positive bacteria classified into viridians group, and have a role in pathogenesis of dental caries. It’s adhesion to the tooth surface is mediated by cell surface proteins, which interact with specific receptor located in tooth pellicle. Glucan binding protein, Glukosyltransferase, and antigen I/II are basic proteins of S. mutans, which have a role in initiating the interaction. A previous study showed that chicken’s IgY can interfere the interaction. Purpose: The objective of this study was to assess the ability of IgY in recognizing the surface molecule of Streptococcus mutans expressed by various serotypes (c, d, e, f) and a strain derived from IPB, Bogor. Method: Western blot was used as a method to determine such capability. Result: The result showed that IgY has a potency to recognize antigen I/II, but not the other proteins on the cell surface of all bacteria tested. Conclusion: The ability of IgY to bind the surface protein, antigen I/II, indicates that this avian antibody could be used as a candidate for anti-adhesion in preventing dental caries.
Isolation and Identification of Indonesian Lactobacillus reuteri strain from Saliva of Young Adults Armelia Sari Widyarman; Stella Pranoto; Citra Fragrantia Theodorea; Endang Winiati Bachtiar; Boy Muchlis Bachtiar
Scientific Dental Journal Vol. 2 No. 2 (2018): May
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v2i2.2840

Abstract

Background: Biofilms are involved in a wide variety of microbial infections, including dental caries and periodontitis. The use of probiotics has been a promising prevention and treatment modality with which to combat biofilm-related diseases in the oral cavity. The probiotic Lactobacillus reuteri has been proven to reduce gingivitis and plaque index inside the oral cavity. These bacteria can be found in the digestive system and also in the human oral cavity. Objective: The aim of this study was to identify L. reuteri in the saliva of Indonesian young adults. Methods: Forty saliva samples were collected from 18–24 year-old Indonesian subjects. DNA extraction was performed, and then, the identification of L. reuteri was accomplished using PCR. Six subjects showed positive results. The positive samples were cultured in Rogosa Agar for 24 hours at 37°C anaerobically. Several single colonies were further cultured separately in broth medium before DNA extraction and PCR identification were performed. The four thickest bands were selected for DNA sequencing. Results: An analysis performed using BLAST showed that two of the L. reuteri strains obtained from the Indonesian saliva isolates had 96% (isolate 3.11) and 95% (isolate 5.14) values. This confirmed the presence of new strains based on average nucleotide identity (ANI). The isolate strains of 3.11 and 5.14 have been registered at DDBJ/EMBL/GenBank under the accession number LC382415 and LC382416, respectively. Conclusion: L. reuteri novel strain can be isolated from the saliva of Indonesian young adults.  Further studies involving biochemical tests and phenotypic analysis are needed to better understand these new L. reuteri strains.
POTENSI CIGARETTE SMOKE CONDENSATE TERHADAP PENINGKATAN PEMBENTUKAN BIOFILM CANDIDA albicans ISOLAT ATCC 10261 Basri A.Gani; Annisa Qashdina Alghassani; Zaki Mubarak; Endang Winiati Bachtiar; Boy M.Bachtiar
Journal of Syiah Kuala Dentistry Society Vol 2, No 1 (2017): JANUARY
Publisher : Dentistry Faculty

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Abstract

Candida albicans (C. albicans) is the main agent in pathogenesis of oral candidiasis, and also the cigarette smoke is one of predisposing factors of C. Albicans virulence, mainly in biofilm formation that it the role as the initiator of adhesiononoral mucosal. This research aimed to identify the effect of Cigarette smoke condensate (CSC) on biofilm formation of C. albicans. Candida albicans was sensitized with CSC kretek and non-kretek that shown the increase of biofilm formation (p0,05) compared control, specifically in 24 hour. Its related with massa of biofilm formation that seem by microscope (400x) and also CSC non kretek better than CSC kretek in biofilm formation of C. albicans, mainly in 24 and 48 hours, that provisional, in 12 hours were the most dominant biofilm figuration of C. albicans sensitized by CSC kretek, although it intensity is lower than 24 hours. The research was concluded that CSC can be trigger to enhancement of biofilm formation of C. albicans in 24 hours and also CSC non kretek better than CSC kretek.Keyword: Candida albicans, Cigarrete smoke condensate, Biofilm
Co-Authors A. Fieki Rachmatillah Afifah Bambang Sutjiatmo, Afifah Bambang Aimee Monica Aimee Monica Anissha D Putri Annisa Qashdina Alghassani Ariadna A Djais Basri A. Gani Basri A.Gani C Hanny Wijaya Dede R. Adawiyah Dewi Fatma Suniarti Elisabeth A Putri Endang W Bachtiar Endang W Bachtiar Endang W Bachtiar Endang Winiati B Endang Winiati Bachtiar Euis Reni Yuslianti Fathia Agzarine Deandra Heriandi Sutadi I. Wayan T. Wibawan Karina Dhaniarti Lukman Hakim Makmun Maryati Maryati Mindya Yuniastuty Mindya Yuniastuty Mochamad Fahlevi Rizal Natalina Nurtami Soedarsono Ria Puspitawati Robert Lesang Santi Chismirina Sari, Bernadeta RE Stella Pranoto Sutjiatmo, Afifah B Theodorea, Citra Fragrantia Widyarman, Armelia Sari Yulianti Kemal Yuslianti, Euis Reni Zaki Mubarak Zinatul Hayati