Hamdani Budiman
Laboratorium Patologi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh

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Histopathological Changes of Erythrocyte and Quantity of immature Erythrocytes in Tegal Duckling (Anas Javanica) Induced by Lead (Pb) Budiman, Hamdani; Aliza, Dwinna
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i1.9792

Abstract

The aim of this research is to find out the histopathological changes of erythrocyte and the quantity of immature erythrocyte of tegal duckling treated with lead (Pb). Twenty five young female ducks with the age of 7 days and 60.55.07 g body weight alloted into 4 treatment groups and 1 control group (Ko) with 5 replication each. The treatment group fed with K1 (5.7), K2 (11.4), K3 (17.1), and K4 (22.8) mg Pb/kg body weight for 10 days, respectively. Young ducks showed clinical symptoms such as the color of feces were brown to black, green color of diarrhea, anemic and decreasing body weight. Histopathologically, erythrocyte showed that immature and abnormal in shape (like teardrops, dumb bell, and bottles). The result of variant analysis followed by linier regression shows that the higher the dosage of Pb in diet the higher the quantity of immature erythrocyte andabnormal erythrocyte shape found (P0.01).
Isolation and Characterization of Avian Influenza Virus Based on Macroscopic Examination of Native Chicken Embryo Fakhrurrazi, Fakhrurrazi; Budiman, Hamdani
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i2.9788

Abstract

The aim of this research was to isolate and characterize the virus based on macroscopic examination of native chickens embryo infected with the AI virus. This research used 10 infected native chickens from Ateuk Pahlawan and Doi villages. All chickens were examined to identify the present of AI using rapid test and in ovo virus propagation. Rapid test showed 7 chickens from Ateuk Pahlawan villageand 5 chickens from Doi village were AI positive. Propagation of the viruses in 11 days old chicken embryo in ovo showed that embryo died 5 days post inoculated whereas macroscopic examination showed dwarfed embryo, hemorrhagic, and loss of feather.
Histopathological Change of White Mouse Ovary as a Result of Ethylene Glycol Intoxication Budiman, Hamdani; Akmal, Muslim; N. Siregar, Tongku
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i2.9782

Abstract

This research was aimed to know histopathology change of white mouse ovary as result of EG intoxication. Eight white mice randomly divided into three treatment groups and one control group. Treatment of P1 group was given 10 ml EG/L drinking water, P2 was given 20 ml EG/L drinking water, and P3 was given 30 ml EG/L drinking water for two weeks. Control group was given aquadest. Then, white mice were euthanized and ovaries sample were taken for histology observation. The result for treatment groups (P1 and P2) had shown ovary hyperemic (congest). But, the change is worst in P2 rather than P1. Changes in P3 treatment group had shown ovary hyperemic (congest) and leukocyte infiltration in follicular antrum and follicular necrosis causing lost of ovum cell (cariolysis). Poisoned of EG in white mouse can cause inflammation and necrosis of ovary follicle thus the formed of ovum cell was failed (ovogenesis).
PROFILE OF ALLERGY HYPERPLASMA PATHOLOGIC ANTIBODY AND IMMUNOGENIC CHARACTERISTIC Nazaruddin, Nazaruddin; Budiman, Hamdani; Gani, Basri A.; Jakfar, Subhaini; Hasan, M.; Hanafiah, Muhammad
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i1.5299

Abstract

The objective of this research was to determine the allergy hyperplasma pathologic antibody and to evaluate the immunogenic characteristics. Blood and serum were collected from human suffering rhinitis, skin, eye and asthma allergies. To obtain a cloned allergen plasma protein (protein allergen that had been induced with the serum from human with allergic rhinitis, skin, eye and asthma), the goat was used as an intermediate animal. Hematological analysis showed that the leukocyte cell such as neutrophil, lymphocyte, monocyte, and eosinophil increase in allergic-suffered human. The blood smear test exhibited that the mastocyte cell was dominant which contributes to allergy activities in human body. The sodium dodecyl sulphate-polyacrylimide gel electrophoresis (SDS-PAGE) assay detected protein allergens with molecular weight of 188 kDa (IgE) and 60-62 kDa (mastocyte cell). The reactivity assay using enzyme linked immunosorbent-assay (ELISA) revealed that cloned-allergens (whole hyperplasma allergen from goat isolates) express the best reactivity at various concentrations of IgE than the leukocyte cells. This research concluded that the clones of protein allergen have better immunogenic characteristic and those proteins can be recommended as the candidate of allergen to induce the humoral immunity on host and deliver specific product of anti-allergy such as milk.
SUPLEMEN BUNGKIL INTI SAWIT TEPUNG DAUN KATUK BERPOTENSI MENINGKATKAN KUALITAS SPERMATOZOA PADA KAMBING PERANAKAN ETTAWA Akmal, Muslim; Reza Ferasyi, Teuku; Budiman, Hamdani; R, Razali; A, Azhari; A, Anwar; Aji Pamungkas, Fitra; Nasution, Saddat; Armansyah, T.; Hambal, Muhammad; S, Syafruddin; Sayuti, Arman
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2638

Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian suplemen bungkil inti sawit (BIS), tepung daun katuk (KAT), dan kombinasi bungkil inti sawit dan tepung daun katuk (BISKAT) terhadap peningkatan kualitas spermatozoa kambing jantan peranakan Ettawa (PE). Dalam penelitian ini digunakan 20 ekor kambing jantan PE, berumur 1,5 tahun dengan berat badan antara 15 -20 kg dan dibagi atas empat kelompok yakni P0, P1, P2, dan P3 yang masing-masing diberi akuades, BIS 100 g/hari/ekor, kombinasi BIS 100 g/hari/ekor dan KAT 15 g/hari/ekor, dan KAT 15 g/hari/ekor. Pemberian perlakuan dilakukan selama 35 hari. Pada hari ke-36 dilakukan kastrasi dan selanjutnya dilakukan pemeriksaan kualitas spermatozoa yang meliputi motilitas, viabilitas, integritas membran, dan abnormalitas. Hasil penelitian menunjukkan bahwa pemberian suplemen kombinasi BISKAT dapat meningkatkan motilitas, viabilitas, integritas membran, dan menurunkan abnormalitas spermatozoa dibanding kelompok kontrol. Disimpulkan bahwa pemberian suplemen kombinasi BISKAT berpotensi meningkatkan kualitas spermatozoa kambing PE.
Gambaran Histologis Tubulus Seminiferus Kambing Kacang Jantan Lokal Pascapemberian Suplemen Daun Katuk Ferasyi, Teuku Reza; Budiman, Hamdani; Akmal, Muslim; Melia, Juli; R, Razali; Novita, Andi; Barus, Rina Aulia; Suprayogi, Agik
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1261

Abstract

Penelitian ini mengetahui pengaruh pemberian tepung atau ekstrak alkohol daun katuk terhadap perubahan histologis testis kambing kacang lokal jantan. Dalam penelitian ini digunakan 15 ekor kambing dengan umur sekitar 1,5 tahun. Semua hewan penelitian terlebih dahulu diaklimatisasi selama 2 minggu terhadap lingkungan penelitian. Hewan-hewan tersebut dibagi menjadi 3 kelompok yang masing-masing terdiri atas 5 ekor kambing. Kelompok P0 (kontrol) telah hanya diberikan air distilasi. Pada kelompok P1, diberikan perlakuan berupa suplementasi tepung daun katuk dan untuk kelompok P2 diberikan ekstrak alkohol daun katuk. Semua perlakuan diberikan secara oral sebanyak dua kali per hari (pada pagi dan sore hari) selama 35 hari berturut-turut. Setiap kambing percobaan diberikan pakan yang terdiri atas daun-daunan dan rumput, serta disediakan akses untuk memperoleh air minum secara ad libitum. Pada akhir waktu penelitian, seluruh hewan dikastrasi dan bagian tubulus seminiferus dari testis diambil dan diproses lebih lanjut untuk evaluasi secara histologis. Hasil yang diperoleh menunjukkan bahwa terdapat indikasi peningkatan produksi spermatid dan spermatosit pada Kelompok P1 dan P2. Dengan demikian disimpulkan bahwa pemberian suplemen daun katuk dapat meningkatkan produksi spermatid dan spermatosit kambing kacang lokal jantan.