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All Journal Jurnal Akuakultur Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Veteriner Teknotan: Jurnal Industri Teknologi Pertanian BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIVITA, Journal of Agricultural Science Jurnal Riset Kimia agriTECH PROSIDING SEMINAR KIMIA Jurnal Ilmiah Manuntung JURNAL ATOMIK Jurnal Jamu Indonesia PERENNIAL ULIN: Jurnal Hutan Tropis Jurnal Riset Teknologi Industri Journal of Tropical Pharmacy and Chemistry Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan Indonesian Journal of Jamu

Irawan Wijaya Kusuma, Irawan Wijaya

Fakultas Kehutanan Universitas Mulawarman

Author-ID : 2473377

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Nursing Public Health Veterinary Other

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UJI FITOKIMIA DAN ANTIOKSIDAN EKSTRAK ETANOL PROPOLIS LEBAH KELULUT (TETRAGONULA IRIDIPENNIS) DARI SAMARINDA KALIMANTAN TIMUR Khairunnisa, Binti; Rosamah, Enih; Kuspradini, Harlinda; Kusuma, Irawan Wijaya; Sukemi, Sukemi; Tandirogang, Nataniel; Arung, Enos Tangke
Jurnal Ilmiah Manuntung Vol 6 No 1 (2020): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung akademi farmasi samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.456 KB)

The study aims to identify the secondary metabolite compounds contained in the ethanol extract Propolis Tetragonula Iridipennis and determine the antioxidant activity. Propolis is extracted from the beehive of Kelulut (Tetragonula Iridipennis) with a method of maceration of ethanol 96% produce a yield of 66.73%. Propolis extract contains alkaloid compounds, flavonoids, triterpenoids, tannins and carbohydrates. Propolis extract tested its antioxidant activity using the DPPH method (1.1-diphenyl-2-picrylhydrazyl) with a wavelength of 514 nm. Results showed a percentage of propolis extract free radicals 59% in concentrations of 100 ppm with IC50 33.74 ppm so that propolis extract is potentially as an antioxidant.  

AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI BAGIAN RANTING DAN BATANG TUMBUHAN KARAMUNTING (Melastoma malabathricum): Antioxidant and Antibacterial Activity of The Twigs and Stem Bark of Karamunting Plants (Melastoma malabathricum) Nur Maulida Sari; Irawan Wijaya Kusuma; Rudianto Amirta; Nur Indriana Fitriah
PERENNIAL Vol. 17 No. 2 (2021): Vol. 17 No. 2, Oktober 2021
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v17i2.14547

East Kalimantan is one of the areas that have the potential for various traditional medicinal plants. Melastoma malabathricum (known locally as karamunting, senduduk) is an invasive plant in the family of Melastomataceae with a potential medicinal plant. This study explored the potential of twigs and stem bark of Karamunting (Melastoma malabathricum) for its antioxidant and antibacterial activity. The twigs and stem bark of Karamunting were macerated to yield ethanolic extract. The phytochemical screening was evaluated by Harborne and Kokate methods. Antioxidant activity was evaluated by DPPH radical scavenging assay. Antibacterial activity was examined using agar well diffusion method against Propionibacterium acnes. The results showed that the ethanol extracts of twigs and stem bark samples display an ability to inhibit DPPH free radical by 82% and 88% at 50 ppm concentration. Antibacterial activity of the twigs and stem bark samples showed potent activity to inhibit the P. acnes growth with 11.3 mm and 11 mm inhibition activity. Based on the results, the twigs and stem bark of M. malabathricum plants display potential as a natural antioxidant and antibacterial agent.

Skrining Fitokimia dan Toksisitas dari Ekstrak Daun Pecut Kuda (Stachytarpheta jamaicenesis (L.) Vahl) Irawan Wijaya Kusuma; Erwin Erwin; Muhammad Wildan Rizqi Miftahussanadi
ULIN: Jurnal Hutan Tropis Vol 5, No 1 (2021)
Publisher : Fakultas Kehutanan Universitas Mulawarman

The Pecut kuda plant is one of the plants of the genus Stachytarpheta which is found in tropical regions, including Indonesia. In Indonesia this plant is found in East Kalimantan which grows wild. This study aims to determine the type of secondary metabolites and the level of toxicity of the leaves of horse whip (Stachytarpheta jamaicenesis (L.) Vahl). The research stages were sample drying, sample maceration, filtered, concentrated with a rotary evaporator, phytochemical and toxicity tests using linear regression values to determine the LC50 value with SAS probit analysis. Phytochemical test results showed that the pecut horse leaf extract in the n-hexane fraction contained flavonoids, quinones and steroids, the ethyl acetate fraction contained alkaloids, steroids, saponins, flavonoids and quinones and the methanol fraction contained phenolic flavonoids, quinones and saponins. The results of the toxicity test on Artemia salina L. shrimp larvae in the fraction of n-hexane, ethyl acetate and methanol obtained LC50 values of 0.036 ppm, 5.24 ppm and 29.51 ppm, respectively. Based on the LC50 value, it can be concluded that the three fractions are very toxic, but the most toxic is the n-hexane fraction (LC50 = 0.036 ppm).

POTENSI JAMUR TIRAM PUTIH (Pleurotus ostreatus) TERHADAP PENGHAMBATAN Candida albicans DAN Propionibacterium acnes Saat Egra; Irawan Wijaya Kusuma; Enos Tangke Arung
ULIN: Jurnal Hutan Tropis Vol 2, No 1 (2018)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Oyster mushroom (Pleurotus ostreatus) is not a new food for the community. Long time ago oyster mushrooms is food but not so well known. Now oyster mushrooms have been accepted by the community as a healthy food. This is the background of this research to produce mushrooms not only as nutritious food but also natural medicine. The purpose of this study was to examine the antimicrobial potential of oyster mushrooms by determination of clear zone against Candida albicans and Propionibacterium acnes bacterias. This research was conducted at Forest Products Chemistry Laboratory, Faculty of Forestry, Mulawarman University. The resources of oyster mushroom we used in this study comes from the cultivation of the entrepreanure forestry student group in Mulawarman University. This study used successful extraction with hexane solvent, ethyl acetate, ethanol, water, and crude ethanol. The results obtained that the highest antimicrobial inhibition against Candida albicans bacteria with 47.60% with a concentration of 100 ppm but, on the antimicrobial against Propionibacterium acnes bacteria did not show any significant inhibition.

Aktivitas Antioksidan, Kandungan Total Fenolik dan Kandungan Total Flavonoid Pada Beberapa Tumbuhan Berkhasiat Obat Di Kalimantan Timur, Indonesia Nur Maulida Sari; Irawan Wijaya Kusuma; Nur Indriana Fitriah
ULIN: Jurnal Hutan Tropis Vol 5, No 2 (2021)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32522/ujht.v5i2.6224

Antioxidant activity, total phenolic and total flavonoid contents of some traditional medicinal plants grown in East Kalimantan, Indonesia, were investigated. Plant sample are Macaranga gigantea leaf, Ceiba pentandra leaf, Ceiba pentandra bark, Blumea balsamifera leaf and Artocarpus altilis leaf. Natural antioxidants from plant species are considered safe. Therefore, people are now more interest in finding out the treatment through natural remedies. The plant's total phenolic content was determined by the Folin-Ciocalteu method, while total flavonoid content was determined by the Colorimetric assay method. Antioxidant activity was evaluated by DPPH radical scavenging assay. The results showed that plant samples tested contained 22.02 to 214.88 µg of gallic acid equivalents (GAE)/mg extract represented the total phenolic content. The flavonoid content of the samples was in range from 50.00 to 896.67 µg of catechin equivalents (CE)/mg extract. The plant samples displayed ability to inhibit DPPH free radical formation by 59-81% at 50 ppm concentration. These finding suggest that these plants could possess natural antioxidants and give a scientific basis to the traditional uses of the investigated plants.

KANDUNGAN ANTIOKSIDAN PADA JAMUR TIRAM PUTIH (Pleurotus ostreatus) Saat Egra; Irawan Wijaya Kusuma; Enos Tangke Arung
ULIN: Jurnal Hutan Tropis Vol 2, No 2 (2018)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Indonesia merupakan negara hutan tropis yang luas, maka tak heran negara ini menjadi negara megabiodiversitas. Jamur tiram merupakan salah satu hasil kekayaan alam Indonesia yang memiliki potensi sebagai tanaman kesehatan dan obat. penelitian ini bertujuan untuk memunculkan potensi aktivitas antioksidan pada jamur tiram yang banyak dibudidaya di Kalimantan Timur khususnya Samarinda. Sampel kami ambil dari rumah jamur borneo dan penelitian ini dilaksanakan di Laboratorium Kimia Hasil Hutan, Fakultas Kehutanan, Universitas Mulawarman. Metode ekstraksi bertingkat menggunakan n-heksan, etil asetat, dan etanol. Metode antioksidan yang digunakan adalah penangkal radikal bebas (DPPH) dengan masing-masing tiga kali pengulangan. Hasil penelitian ini menunjukkan bahwa aktivitas antioksidan tertinggi pada ekstrak air dengan nilai penghambatan 25% pada kosentrasi 100 ppm. Pada ekstrak heksan merupakan hambatan terkecil dengan nilai penghambatan 2,41% pada kosentrasi 25ppm.

The Toxicity and Antibacterial Properties of Calamus ornatus Bl. Rattan Fruit Heriad Daud Salusu; Ernita Obeth; Abdul Rasyid Zarta; Eva Nurmarini; Hamka Nurkaya; Irawan Wijaya Kusuma; Enos Tangke Arung
agriTECH Vol 39, No 4 (2019)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Calamus ornatus Bl., locally known as rotan mantang or rotan seuti is one of the commercial rattan species growing in the forests of East Borneo. Its fruit is edible, like the fruits of other species of rattan, and it has been used by the local people as traditional medicine to treat stomachache or toothache. Until recently, there has not been any study investigating the benefits of the fruit for medicinal or other purposes. This research aimed to discover the bio-activity potentials of Calamus ornatus Bl. fruits by identifying its toxicity level and antibacterial potentials. The parts of the fruit (pericarp, flesh, and seed) were extracted using ethanol as solvent to extract samples. The toxicity was analyzed by using the Brine Shrimp Lethality Test (BSLT) method while the antibacterial activity was tested using a well diffusion method. Low toxicity was found in the flesh, whereas the pericarp and the seed were non-toxic. Antibacterial activity against Escherichia coli was found in the pericarp and the seed, while antibacterial activity against Streptococcus mutans was found in the pericarp and the flesh.

UJI IN VITRO GABUNGAN EKSTRAK TANAMAN DARI KALIMANTAN, INDONESIA TERHADAP BAKTERI PATOGEN PADA IKAN NILA Esti Handayani Hardi; Gina Saptiani; Nurkadina Nurkadina; Irawan Wijaya Kusuma; Wiwin Suwinarti
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of this study was to evaluate the antibacterial activity of ethanol concoction (mixed extract) of three herbs plants, such as: Boesenbergia pandurata, Solanum ferox and Zingimber zerumbet against Aeromonas hydrophila, Pseudomonas sp. and both bacteria. The Plant extracts were obtained by using ethanol and the concentration of the extract was 600 mgL-1 of B. pandurata, 900 mgL-1 of S. ferox and 200 mgL-1 of Z. zerumbet. The inhibition zone was measured after 6, 12, 18, 24 h incubation at 30o C. The ratios of concoction S. ferox and B. pandurata (for 100 mL solution) were 90:10; 80:20; 70:30; 60:40; 50:50; 40:60; 30:70; 20:80; 10:90. The same ratios were made on the concoction of S. ferox and Z. zerumbet. The concoctions of S. ferox and B. pandurata in the ratio of 50:50 and 60:40, and the concoction of S. ferox and Z. Zerumbet with ratio 60:40 had higher antibacterial activity against A. hydrophila single isolate compared to the other concoctions. Furthermore, the concoction extract of S. ferox and B. pandurate 50:50 and the combined of S. ferox and Z. zerumbet ratio 50:50 and 90:10 were the best combination to inhibit the growth of a single bacterium Pseudomonas sp. The combined S. ferox and B. pandurata ratio 50:50; 10:90 and S. ferox mixed with Z. zerumbet ratio 50:50 and 40:60 were the best combination against the combined bacteria between A. hydrophila and Pseudomonas sp. The conclusion of this research was the combined extract of S. ferox and B. pandurata and S. ferox with Z. zerumbet are effective to suppress the growth of single or combination of A. hydropila and Pseudomonas sp.

SKRINING FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL KAYU BATANG MENTAWA (Artocarpus anisophyllus Miq.) Octariana Toding Pabita; Erwin Erwin; Irawan Wijaya Kusuma
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2019
Publisher : PROSIDING SEMINAR KIMIA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Penelitian mengenai uji fitokimia dan aktivitas antioksidan pada ekstrak batang Mentawa (A. anisophyllus Miq.) telah dilakukan. Penelitian ini bertujuan untuk mengetahui kandungan senyawa metabolit sekunder dan nilai aktivitas antioksidan terhadap radikal bebas DPPH (2,2-diphenyl-1-picrylhidrazil) pada ekstrak metanol dari batang mentawa. Hasil uji fitokimia menunjukkan bahwa ekstrak metanol pada batang mentawa (A. anisophyllus Miq.) mengandung golongan senyawa flavonoid, fenolik dan triterpenoid dan hasil uji aktivitas antioksidan menunjukkan nilai IC50 ekstrak metanol yaitu 34.58 ppm. Hasil pengujian dengan menggunakan metode DPPH menunjukkan bahwa ekstrak kasar dikategorikan sebagai antioksidan sangat kuat. Kata Kunci : Mentawa, fitokimia, antioksidan, dan DPPH

PREVENTION METHODS AGAINST Aeromonas hydrophila and Pseudomonas fluorescens INFECTION IN TILAPIA Esti Handayani Hardi; Rudi Agung Nugroho; Irawan Wijaya Kusuma; Wiwin Suwinarti; Ryan Anjani; Aziza Aziza
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

This research evaluated a method involving provision of a concoction of Boesenbergia pandurata, Solanum ferox dan Zingimber zerumbet extracts for pathogen prevention in tilapia. The concentration of each extract was 600 ppm of Boesenbergia pandurata/BP, 900 ppm of Solanum ferox/SF and 200 ppm of Zingimber zerumbet/ZZ. The examination was performed by issuing two combinations of extracts (SF:BP, SF:ZZ) against Aeromonas hydrophila and Pseudomonas fluorescens (105 CFUmL-1). Preventive trials were carried out by providing a concoction of extracts through intraperitoneal injection (0.1 mL/fish) in tilapia (15Â±2 g) and the immersion method was performed by bathing the fish in the extracts for 20 minutes, with pathogen challenging during the following 24 h being carried out. The composition of the used extract was by SF60:ZZ40; SF50:ZZ50; BP90:SF10; BP50:SF50; and fish without being given the extract. Haematology and immunology parameters were observed at the 4th week after challanges with pathogenic bacteria. The number of white blood cells (WBCs) increased significantly (P <0.05) compared to controls without extract, with a similar increase observed for red blood cell (RBCs), but heamatocrit (Ht) and hemoglobin (Hb) values did not significantly increase compared to control. Phagocytic index, respiratory burst and lysozyme activities also experienced a significant increase in fish fed with combined extracts compared to controls. The numbers of pathogenic bacteria in the body of the fish given extract were also lower than the control and significantly different at the 4th week. The results of this study indicate that giving combined extracts of SF50:ZZ50 and BP90:SF10 provides the best protection (RPS) against infection of A. hydrophila and P. fluorescent by injection of 100%. This study indicates that providing combined extracts by injection and immersion in the ratio of SF50:ZZ50 has a positive effect in increasing the non-specific immune system of tilapia and increasing protection against bacterial infections.

Co-Authors Abdul Rasyid Zarta Abdul Rasyid Zarta Agriandini, Maulina Agus Sulistyo Budi Arief Heru Prianto Assegraff , Andi Syahid Khalid Aziza Aziza Azizatul Karimah Bayu Susetya Ananda Binti Khairunnisa Dyah Ayu Dewi Listyaningrum Edy Budiarso Edy Budiarso Enih Rosamah Enos Tangke Arung, Enos Tangke Ernita Obeth Erwin Erwin Erwin Erwin Erwin Erwin Esti Handayani Hardi Eva Nurmarini Fahriya Puspita Sari Faizatul Falah Farida Ariani Farida Aryani Farida Fitriani Purba Gina Saptiani Hamka Nurkaya Harlinda Kuspradini Heriad Daud Salusu Heriad Daud Salusu Heriad Daud Salusu Hetty Manurung Isna Yuniar Wardhani Khairunnisa, Binti Komsanah Sukarti Marjenah Marjenah Mark Rayment Muhammad Adly Rahandi Lubis Muhammad Akmal Rizqullah Muhammad Wildan Rizqi Miftahussanadi Nataniel Tandirogang Nissa Nurfajrin Solihat Nugroho, Rudi Agung Nur Indriana Fitriah Nur Indriana Fitriah Nurkadina Nurkadina Octariana Toding Pabita Rico Ramadhan, Rico Rikna Naila Salsabila Rita Rusman Rosamah, Enih Rudi Agung Nugroho Rudianto Amirta Ryan Anjani Saat Egra Sarah Azzara Dikarulin Sari, Nur Maulida Sukemi Sukemi Suroto HS Suwinarti, Wiwin Tasya Ananda Putri Wawan Kustiawan Whicliffe Fiernaleonardo Pasedan Widya Fatriasari Wita Pradiani Yuliansyah Yuliansyah Yundari, Yundari Zuliani, Nur Endah

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