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All Journal Jurnal Akuakultur Indonesia Jurnal Veteriner Teknotan: Jurnal Industri Teknologi Pertanian BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIVITA, Journal of Agricultural Science Jurnal Riset Kimia agriTECH PROSIDING SEMINAR KIMIA JURNAL ATOMIK Gorontalo Journal of Forestry Research Jurnal Jamu Indonesia PERENNIAL ULIN: Jurnal Hutan Tropis Jurnal Riset Teknologi Industri Journal Of Medicine And Health Sciences (Medisci) Journal of Tropical Pharmacy and Chemistry Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan Indonesian Journal of Jamu
Irawan Wijaya Kusuma, Irawan Wijaya
Fakultas Kehutanan Universitas Mulawarman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Nursing Public Health Veterinary Other

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Aktivitas Antioksidan dan Antibakteri Ekstrak Daun Pometia pinnata Kuspradini, Harlinda; Pasedan, Whicliffe Fiernaleonardo; Kusuma, Irawan Wijaya
Jurnal Jamu Indonesia Vol. 1 No. 1 (2016): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v1i1.5

Abstract

Penelitian ini bertujuan menentukan aktivitas antioksidan dan antibakteri berbagai jenis ekstrak daun Pometia pinnata. Kegiatan yang dilakukan meliputi proses ekstraksi daun Pometia pinnata dari Kalimantan Timur dengan teknik maserasi bertingkat menggunakan 3 pelarut: n-heksana, etil asetat, dan etanol. Ekstrak tersebut kemudian dievaluasi aktivitas antioksidan dan antimikrobanya.Dari hasil penelitian menunjukkan bahwa ekstrak daun P. pinnata mengandung alkaloid, tannin, dan kumarin. Hasil pengujian antioksidan dengan metode peredaman radikal bebas DPPH, pada konsentrasi 100 ppm menunjukkan aktivitas antioksidan yang tinggi dengan nilai 89,23%, 89,23% dan 90,38% pada masing-masing ekstrak n-heksana, etil asetat dan etanol. Nilai antioksidan menunjukkan nilai yang hampir sama dengan antioksidan vitamin C sebesar 96 pada konsentrasi 25. Hasil pengujian antimikroba dengan metode pewarnaan 2, 3, 5-triphenyl tetrazolium chloride (TTC) menunjukkan bahwa ekstrak daun P. pinnata memiliki kemampuan yang cukup kuat dalam menghambat pertumbuhan bakteri Streptococcus mutans, Streptococcus sobrinus, Escherichia coli kecuali terhadap Propionibacterium acne.
PREVENTION METHODS AGAINST Aeromonas hydrophila AND Pseudomonas fluorescens INFECTION IN TILAPIA Hardi, Esti Handayani; Nugroho, Rudi Agung; Kusuma, Irawan Wijaya; Suwinarti, Wiwin; Anjani, Ryan; Aziza, Aziza
BIOTROPIA Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1425.045 KB) | DOI: 10.11598/btb.0.0.0.1131

Abstract

This research evaluated a method involving provision of a concoction of Boesenbergia pandurata, Solanum ferox dan Zingimber zerumbet extracts for pathogen prevention in tilapia. The concentration of each extract was 600 ppm of Boesenbergia pandurata/BP, 900 ppm of Solanum ferox/SF and 200 ppm of Zingimber zerumbet/ZZ. The examination was performed by issuing two combinations of extracts (SF:BP, SF:ZZ) against Aeromonas hydrophila and Pseudomonas fluorescens (105 CFUmL-1). Preventive trials were carried out by providing a concoction of extracts through intraperitoneal injection (0.1 mL/fish) in tilapia (15±2 g) and the immersion method was performed by bathing the fish in the extracts for 20 minutes, with pathogen challenging during the following 24 h being carried out. The composition of the used extract was by SF60:ZZ40; SF50:ZZ50; BP90:SF10; BP50:SF50; and fish without being given the extract. Haematology and immunology parameters were observed at the 4th week after challanges with pathogenic bacteria. The number of white blood cells (WBCs) increased significantly (P <0.05) compared to controls without extract, with a similar increase observed for red blood cell (RBCs), but heamatocrit (Ht) and hemoglobin (Hb) values did not significantly increase compared to control. Phagocytic index, respiratory burst and lysozyme activities also experienced a significant increase in fish fed with combined extracts compared to controls. The numbers of pathogenic bacteria in the body of the fish given extract were also lower than the control and significantly different at the 4th week. The results of this study indicate that giving combined extracts of SF50:ZZ50 and BP90:SF10 provides the best protection (RPS) against infection of A. hydrophila and P. fluorescent by injection of 100%. This study indicates that providing combined extracts by injection and immersion in the ratio of SF50:ZZ50 has a positive effect in increasing the non-specific immune system of tilapia and increasing protection against bacterial infections.
AKTIVITAS ANTIOKSIDAN DAN ANTIMIKROBA SARANG BURUNG WALET (Collocalia Fuciphaga) DARI BERBAGAI JENIS KELAS SARANG DAN JENIS BANGUNAN Andi Syahid Khalid Assegraff; Wiwin Suwinarti; Isna Yuniar Wardhani; Agus Sulistyo Budi; Irawan Wijaya Kusuma; Enos Tangke Arung
Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan Vol. 10 No. 2 (2024): Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v10i2.804

Abstract

The community has traditionally utilized the swallow’s nest (Collocalia fuciphaga), an edible bird’s net (EBN), as a component in medicines and cosmetics. Within the cave’s walls lies the environment for the swallow’s nest. The nest’s quality and form are taken into account when determining the nest wallet’s selling price. This study intends to ascertain the possible suppression of the bacteria Staphylococcus sp. and Escherichia sp. activity as well as the antioxidant activity of the swallow’s nest from the nest class formed from wood and concrete construction types. The study was conducted directly on dry, hair- and dirt-free, finely powdered, and dissolved in distilled water swallow nests (Collocalia fuciphaga). Testing for antibacterial activity was conducted. Agar well testing for antibacterial activity was carried out using Mueller Hinton Agar (MHA) and chloramphenicol media as positive controls. Ascorbic acid was used as a positive control while 1,1-diphenyl-2-picrylhydrazyl (DPPH) was tested for its ability to scavenge free radicals. The test findings demonstrated that the swallow (Collocalia fuciphaga) had substantial antioxidant activity against DPPH and that the swallow’s nest (Collocalia fuciphaga) had antibacterial activity against Staphylococcus sp. and Escherichia sp. germs. The swallow’s nest’s (Collocalia fuciphaga) antioxidant and antibacterial properties are specific to different nest classes and building kinds, so they may be utilized as a foundation for thought about raising the selling price of bowls, powders, and crumbles in each form of structure.
Co-Authors Abdul Rasyid Zarta Abdul Rasyid Zarta Agriandini, Maulina Agus Sulistyo Budi Amirta, Rudianto Ananda, Bayu Susetya Andi Syahid Khalid Assegraff Anjani, Ryan Arief Heru Prianto Aziza, Aziza Azizatul Karimah Binti Khairunnisa Dewi Listyaningrum, Dyah Ayu Dikarulin, Sarah Azzara Dungani, Rudi Edy Budiarso Edy Budiarso Enih Rosamah Enos Tangke Arung Enos Tangke Arung, Enos Tangke Ernita Obeth Erwin Erwin Erwin Erwin Erwin Erwin Esti Handayani Hardi Eva Nurmarini Fahriya Puspita Sari Faizatul Falah Farida Ariani Farida Aryani Farida Fitriani Purba Gina Saptiani Hamka Nurkaya Harlinda Kuspradini Heriad Daud Salusu Heriad Daud Salusu Heriad Daud Salusu Isna Yuniar Wardhani Khairunnisa, Binti Komsanah Sukarti Mark Rayment Muhammad Adly Rahandi Lubis Muhammad Akmal Rizqullah Muhammad Wildan Rizqi Miftahussanadi Nataniel Tandirogang Nissa Nurfajrin Solihat Nugroho, Rudi Agung Nur Indriana Fitriah Nur Indriana Fitriah Nurkadina Nurkadina Octariana Toding Pabita Pasedan, Whicliffe Fiernaleonardo Putri, Tasya Ananda Rico Ramadhan, Rico Rikna Naila Salsabila Rita Rusman Rosamah, Enih Rosamah, Enih Rudianto Amirta Saat Egra Sari, Ayu Mitha Sari, Nur Maulida Sukemi Sukemi Suroto HS Suwinarti, Wiwin Whicliffe Fiernaleonardo Pasedan Wicaksono, Dian Anggoro Widya Fatriasari Wita Pradiani Yani, Sinar Yuliansyah Yuliansyah Yundari, Yundari Zuliani, Nur Endah