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All Journal Jurnal Akuakultur Indonesia Jurnal Veteriner Teknotan: Jurnal Industri Teknologi Pertanian BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIVITA, Journal of Agricultural Science Jurnal Riset Kimia agriTECH PROSIDING SEMINAR KIMIA JURNAL ATOMIK Gorontalo Journal of Forestry Research Jurnal Jamu Indonesia PERENNIAL ULIN: Jurnal Hutan Tropis Jurnal Riset Teknologi Industri Journal Of Medicine And Health Sciences (Medisci) Journal of Tropical Pharmacy and Chemistry Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan Indonesian Journal of Jamu

Irawan Wijaya Kusuma, Irawan Wijaya

Fakultas Kehutanan Universitas Mulawarman

Author-ID : 2473377

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Nursing Public Health Veterinary Other

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Aktivitas Anti Mikroba Dari Ekstrak Bawang Tiwai Suroto HS; Irawan Wijaya Kusuma
Jurnal Riset Teknologi Industri Vol 3 No 5 Juni 2009
Publisher : Balai Riset dan Standardisasi Industri Samarinda

Bawang tiwai (Eleutherine americana L. Merr) has chemical compound such as carbohydrate, protein, fat, alkaloid, flavonoid, carbocylate acid, aldehyde keton, thanin and phenol. Bawang tiwai is used by the ethnic of Dayak in Eas Kalimantan for spicy and medicine. Information about this chemical compound should be studied further and one of the study is to know the characteristic and activity of bawang tiwai as antimicrobial activity. The extract of bawang tiwai using methanol solvent has characteristic of brown concentrated solid (gummy), non crystalin, non aromatic and the extract of bawang tiwai using the solvent of n-tiexene. dietil ether and elit acetate has a characteristic of yellow brownies solid, bit oil, and both extractives are dissolved in acetone and methanol. Repeated seperetion with hexen, etil acetate, benzene-acetone using a coloum chromatography resulted in old yellow to brown fraction and aromatic. The fraclions resulted by step one and two separation are able to resist the growth of salmonella thypi and Bacillus cereus.

Pengaruh Lama Penyimpanan dan Pengenceran Lindi Hitam Terhadap Karakteristik Lignin Kraft Acacia mangium Faizatul Falah; Rikna Naila Salsabila; Wita Pradiani; Azizatul Karimah; Muhammad Adly Rahandi Lubis; Arief Heru Prianto; Nissa Nurfajrin Solihat; Fahriya Puspita Sari; Rita Rusman; Irawan Kusuma Wijaya; Widya Fatriasari
Jurnal Riset Kimia Vol. 13 No. 2 (2022): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v13i2.506

This study aimed to investigate the effects of storage time and dilution of black liquor (BL) from Acacia mangium kraft pulping on the characteristics of isolated Lignin. Lignin isolation was carried out by 1 and 2 steps of isolation using HCL 1M to precipitate Lignin, diluted before isolation. Isolated Lignin was analyzed for its acid-soluble Lignin (ASL), insoluble acid lignin (AIL), functional groups by FTIR, solubility in dioxane and NaOH and thermal properties. The effect of BL storage time was also evaluated on the characteristics of the Lignin produced. The results suggest that the longer BL is stored, the higher the lignin yield. When compared to the isolation approach without dilution, the dilution process produced a higher yield and ash content up to 84% and 21%, respectively. Without dilution, the AIL isolated was lower than the dilution during BL storage. The longer the storage duration, the higher the lignin purity. Compared to two-step lignin isolation, dilution treatment in single-step isolation improves yield and purity. The thermal stability of lignin isolation without dilution (184 ⁰C and 167 ⁰C for 1 and 2 steps, respectively) was higher than that of isolated Lignin with dilution (154.8 ⁰C and 160.9 ⁰C for 1 and 2 steps, respectively), according to thermal study. Both lignin isolates with and without dilution have comparable functional groups, as shown by FTIR spectra. Due to the high yield and purity of isolated Lignin, BL dilution could be a viable alternative in lignin isolation from BL. Moreover, the properties of isolated Lignin are also influenced by BL storage.

Karakteristik dan Aktivitas Antimikroba Minyak Atsiri Daun Actinodaphne borneensis Terhadap Mikroorganise Penyebab Karies Gigi Muhammad Akmal Rizqullah; Farida Fitriani Purba; Irawan Wijaya Kusuma; Harlinda Kuspradini
Teknotan: Jurnal Industri Teknologi Pertanian Vol 17, No 2 (2023): TEKNOTAN, Agustus 2023
Publisher : Fakultas Teknologi Industri Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jtvol17n2.6

Pencarian bahan alami sebagai alternatif pengobatan terhadap infeksi penyebab karies gigi terus dilakukan, salah satunya yaitu menggunakan minyak atsiri. Actinodaphne borneensis merupakan spesies tumbuhan hutan penghasil minyak atsiri dari famili Lauraceae yang tersebar luas di hutan Borneo khususnya Kalimantan Timur. Penelitian ini bertujuan untuk menganalisis karakteristik minyak atsiri daun A. borneensis yang dihasilkan dan mengetahui potensi aktivitas antimikroba terhadap bakteri dan jamur penyebab karies gigi. Minyak atsiri daun A. borneensis diisolasi dengan distilasi water and steam distillation. Minyak atsiri yang diperoleh diuji sifat fisik dan dilakukan identifikasi senyawa penyusunnya menggunakan GC-MS. Aktivitas antimikroba diuji menggunakan metode difusi agar dengan konsentrasi uji 100%, 10% dan 1%. Empat mikroorganisme uji yang digunakan antara lain bakteri Staphylococcus aureus, Streptococcus mutans, Streptococcus sobrinus dan jamur Candida albicans. Hasil karakteristik minyak atsiri yang dihasilkan menunjukkan rendemen sebesar 0,1507%, berwarna kuning, nilai indeks bias 1,441 dan larut dalam alkohol 1:2,4 bagian. Berdasarkan hasil analisis GC-MS menunjukkan komponen kimia penyusun minyak atsiri yang mendominasi diantaranya spathulenol, β-ocimene, (+)-aromadendren, D-limonene dan epiglobulol. Aktivitas antimikroba tumbuhan A. borneensis berpotensi dapat menghambat pertumbuhan S. aureus, S. mutans, S. sobrinus dan C. albicans, dengan zona hambat masing-masing 15,11, 19,78, 20,56, dan 16,77 mm.

Antioxidant Assay of the Ethanolic Extract of Three Species of Rattan Fruits using DPPH Method Heriad Daud Salusu; Farida Aryani; Edy Budiarso; Irawan Wijaya Kusuma; Enos Tangke Arung; Abdul Rasyid Zarta
Journal of Tropical Pharmacy and Chemistry Vol. 4 No. 4 (2018): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v4i4.170

The bioactivity of some species of rattan fruit has been previously studied and it was found that it has potential antioxidant agents. This study was conducted to determine the antioxidant content in three species of edible rattan fruit, namely Calamus manan Miq., Calamus ornatus Bl. and Calamus caesius Bl by using the DPPH method, whereby the test was carried out on the pericarp, flesh, and seeds of each fruit. The research findings revealed that phytochemical compounds, i.e. flavonoids, tannins, and triterpenoids are found almost in all parts of the fruit, while steroid is not found in any of the three species of the fruit. The antioxidant activity found in the fruit of C. caesius Bl is strong, meanwhile the antioxidant activity in the fruit of C. manan Miq only strong in its pericarp and seeds, but it is moderate in its flesh. The antioxidant activity found in the fruit of C. ornatus Bl. is strong in its pericarp and seeds, but it is moderate in its flesh. Therefore, these three species of rattan fruit are potential as antioxidant agents.

UJI FITOKIMIA DAN ANTIOKSIDAN EKSTRAK ETANOL PROPOLIS LEBAH KELULUT (Tetragonula iridipennis) DARI SAMARINDA KALIMANTAN TIMUR Binti Khairunnisa; Enih Rosamah; Harlinda Kuspradini; Irawan Wijaya Kusuma; Sukemi Sukemi; Nataniel Tandirogang; Enos Tangke Arung
Jurnal Ilmiah Manuntung Vol 6 No 1 (2020): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v6i1.309

The study aims to identify the secondary metabolite compounds contained in the ethanol extract Propolis Tetragonula Iridipennis and determine the antioxidant activity. Propolis is extracted from the beehive of Kelulut (Tetragonula Iridipennis) with a method of maceration of ethanol 96% produce a yield of 66.73%. Propolis extract contains alkaloid compounds, flavonoids, triterpenoids, tannins and carbohydrates. Propolis extract tested its antioxidant activity using the DPPH method (1.1-diphenyl-2-picrylhydrazyl) with a wavelength of 514 nm. Results showed a percentage of propolis extract free radicals 59% in concentrations of 100 ppm with IC50 33.74 ppm so that propolis extract is potentially as an antioxidant.

The comparative studies of Borneo plant extracts to increases vaccine efficacy in tilapia, Oreochromis niloticus Hardi, Esti Handayani; Sukarti, Komsanah; Agriandini, Maulina; Kusuma, Irawan Wijaya; Nugroho, Rudi Agung
Jurnal Akuakultur Indonesia Vol. 17 No. 2 (2018): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study was investigated the adjuvant effect of Boesenbergia pandurata (BP), Zingiber zerumbet (ZZ), Solanum ferox (SF) on protection of tilapia with injection Pseudomonas sp. (Pseumulvacc) vaccination. The extract concentrations of BP (600 mg/L), ZZ (200 mg/L), and SF (900 mg/L) were combined with the vaccine, ratio between vaccine and extract was 1:1. Tilapia fish (weight 15 g) were intraperitoneally injected with vaccine mix the extract and challenged at days 7 (d7), 14 (d14), and 21 (d21) post vaccination through intramuscular injection with Aeromonas hydrophila and Pseudomonas fluorescens (105 CFU/mL each pathogen bacteria). The results shown that the fish with BP+V were found in fin rot at d14 days challenge. The same symptoms was found in ZZ+V at d14 challenge as much 11.11% and 42.86%. while, in the vaccine groups (V), after the challenge, tilapia were found fin rot and darkness color until the last experiment. The BP+V and SF+ZZ+V groups shown reducing the number of bacteria in the fish body after challenge test on d7, d14, and d 21. The efficacy of Pseumulvacc vaccine has increased after its administration with BP (BP+V) on day 7 and day 14 after challenge (90%) and 100% at the time of challenge test d21. The conclusion is B. pandurata extract might be a promising adjuvant candidate for fish vaccination, and B. pandurata extract is the best plants as an adjuvant that mixed with the vaccine to against A. hydrophila and P. fluorescens infection. Keywords: Adjuvant, plant extract, vaccine, fish pathogen bacteria ABSTRAK Penelitian ini bertujuan untuk mengevaluasi efek adjuvan dari ekstrak tanaman temu kunci (Boesenbergia pandurata/BP), lempuyang (Zingiber zerumbet/ZZ), dan terung asam (Solanum ferox/SF) pada ikan nila yang diberikan bersama dengan vaksin bakteri Pseudomonas sp. (Pseumulvacc) melalui injeksi. Dosis yang digunakan yaitu ekstrak BP 600 mg/L, ZZ 200 mg/L, dan SF 900 mg/L, dengan rasio antara vaksin dan ekstrak adalah 1:1. Pengujian diawali dengan menginjeksi ikan nila (bobot tubuh 15 g) melalui intraperitoneal dengan campuran vaksin dan ekstrak tanaman, dilanjutkan dengan uji tantang pada hari 7 (d7), 14 (d14) dan 21 (d21) pascavaksinasi dengan bakteri gabungan Aeromonas hydrophila dan Pseudomonas fluorescens (kepadatan bakteri masing-masing 105 CFU/mL) melalui intramuskular. Hasil pengujian menunjukkan bahwa ikan yang diberi vaksin dengan penambahan ekstrak BP (BP+V) masih ditemukan mengalami sirip gripis pada waktu uji tantang hari ke 14, gejala serupa juga ditemukan pada pemberian vaksin yang dicampur dengan ZZ (ZZ+V) pada waktu uji tantang d14, sebesar 11.11 % dan 42.86%. Sedangkan ikan yang diberi vaksin tanpa campuran ekstrak (V) masih ditemukan ikan mengalami sirip gripis dan warna menghitam pada waktu uji tantang d14. Pada perlakuan BP+V dan SF+ZZ+V mampu mengurangi jumlah bakteri di dalam tubuh ikan nila pasca uji tantang d7, d14 dan d21, dan jumlahnya lebih rendah dibandingkan dengan perlakuan lainnya. Efikasi vaksin Pseumulvacc mengalami peningkatan pada BP+V pada hari 7 dan hari 14 pasca ujitantang mencapai 90%, dan 100% pada waktu uji tantang d21. Semakin lama waktu uji tantang (d21), berdampak pada tingkat perlindungan vaksin plus ekstrak yang makin tinggi dibandingkan dengan waktu uji tantang pada hari d7 dan d14. Ekstrak B. pandurata adalah tanaman terbaik sebagai adjuvan yang penggunaannya dicampur dengan vaksin untuk penanggulangan infeksi bakteri A.hydrophila dan P. fluorescens. Kata kunci: Adjuvan, ekstrak tanaman, vaksin, patogen pada ikan

TEST OF ANTIOXIDANT ACTIVITY (DPPH METHOD) OF METHANOL EXTRACT AND ITS FRACTIONS FROM LEAVES OF KNOBWEED (Hyptis capitata Jacq.) Zuliani, Nur Endah; Erwin, Erwin; Kusuma, Irawan Wijaya
JURNAL ATOMIK Vol 4 No 1 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.99 KB)

Test of antioxidant activity of methanol extract and its fractions from leaves of Knobweed (Hyptis capitata Jacq.) were carried out. This research was conducted to determine antioxidant activity in the fractions resulting from column chromatography separation. Antioxidant activity test was carried out by DPPH method while phytochemical was carried out by test colour of TLC method. The results of the first column chromatographic separation produced 18 fractions. The results of the first column antioxidant activity test showed that the value of antioxidant activity ranged from 0.923-81.849% at a sample concentration of 100 ppm. The second column chromatographic separation was carried out in fraction 16. The results of the second column antioxidant activity test produced 18 fractions. The results of the second column antioxidant activity test showed that the value of antioxidant activity ranged from 10.384-59.769% at a sample concentration of 50 ppm. The second column antioxidant activity test produced fraction 15 and fraction 16 showed IC50 values ??of 40.321 and 56.812 ppm respectively. Phytochemical test results showed that fraction 15 and fraction 16 contained alkaloid compounds, flavonoids, polyphenols and steroids/terpenoids. Keywords: Antioxidant, knobweed (Hyptis capitata Jacq), column chromatography, antioxidants, DPPH, secondary metabolites

Chemical Compound Of Ethanol Extract From Seluang Malik (Leea Amabilis Veitch Ex Mast) Potential As An Anti-Diabetes Rosamah, Enih; Kusuma, Irawan Wijaya; Arung, Enos Tangke; Wicaksono, Dian Anggoro; Dungani, Rudi
Jurnal Medisci Vol 3 No 1 (2025): Vol 3 No 1 August 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/medisci.v3i1.941

Background. The Bahau ethnic group in East Kalimantan has employed the roots and leaves of the L. amabilis plant as an anti-diabetic remedy. Thus, scientific verification of this herb is required. Aims. The goal of this study is to examine the bioactive compounds, the anti-diabetic potential, and the chemical composition of L. amabilis ethanolic extracts. Methods. Phytochemical analyses, -amylase enzyme activity assays, DPPH free radical scavenging activity tests, and GC/MS chemical compound analyses were used as experimental methodologies. Result. The findings demonstrated the presence of flavonoids, tannins, steroids, carbohydrates, and carotenoids in the leaf extracts. Alkaloid, tannin, triterpene, and carbohydrates were present in the root extracts. Alkaloid, tannin, triterpene, and carbohydrates were present in the root extracts. The antioxidant and anti-diabetic activity of the L. amabilis leaves extract is higher than that of the root. A significant chemical in the leaves and roots of L. amabilis has been identified as endo-Borneol. Conclusion. A substantial chemical in the leaves and roots of L. amabilis has been identified as endo-Borneol. Endo-Borneol from leaves of L. amabilis showed a chromatographic peak at R. Time of 7.327, area of 2483598, area% of 43.64%, height of 956167, and A/H of 2.60. Nevertheless, the Endo-Borneol chromatographic peak from the root of L. amabilis was found at R. Time of 7.605, area of 889232, area% of 100%, height of 49189, and A/H of 18.08. Implementation. The findings of the study highlight the need for further processing of natural antioxidant and anti-diabetic compounds made from L. amabilis leaves.

Uji aktivitas antibakteri ekstrak propolis lebah Heterotrigona itama dari beberapa lokasi budidaya di Kalimantan Timur terhadap bakteri Propionibacterium acnes Arung, Enos Tangke; Dikarulin, Sarah Azzara; Dewi Listyaningrum, Dyah Ayu; Ananda, Bayu Susetya; Putri, Tasya Ananda; Amirta, Rudianto; Yuliansyah, Yuliansyah; Kusuma, Irawan Wijaya; Ramadhan, Rico; Rosamah, Enih; Suwinarti, Wiwin
ULIN: Jurnal Hutan Tropis Vol 6, No 2 (2022)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32522/ujht.v6i2.7824

Propolis merupakan produk penting bagi lebah yang banyak digunakan sebagai salah satu alternatif pengobatan alami karena mengandung senyawa bioaktif yang dapat memberikan efek positif pada tubuh. Sebagai produk yang dihasilkan lebah untuk menutupi sarangnya dan mencegah predator maupun infeksi bakteri atau virus dari luar, propolis memiliki salah satu manfaat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak propolis lebah Heterotrigona itama yang berasal dari 4 lokasi budidaya (perkebunan, persawahan, hutan sekunder, dan perkotaan) di Kalimantan Timur terhadap bakteri Propionibacterium acnes atau P.acnes dengan metode difusi lubang/sumuran pada media nutrient broth (NB) dengan kloramfenikol sebagai kontrol positif dan aseton sebagai kontrol negatif. Propolis diekstrak menggunakan metode maserasi dengan larutan etanol 96% yang kemudian dibuat dalam 3 (tiga) konsentrasi yaitu 500ppm, 250 ppm, dan 125 ppm. Hasil penelitian menunjukanfitokimia dari propolis di 4 lokasi budidaya dominan mengandung alkaloid, flavonoid dan kumarin. Uji bakteri pada konsentrasi 500 ppm,propolis H.itama dari lokasi perkebunan dan persawahan tergolong dalam kategori kuat dalam menghambat pertumbuhan bakteri, dari lokasi hutan sekunder tergolong sedang, dan dari lokasi perkotaan tergolong rendah.

ANTIBACTERIAL ACTIVITY Homotrigona apicalis PROPOLIS Sari, Ayu Mitha; Kusuma, Irawan Wijaya; Yani, Sinar; Arung, Enos Tangke
Gorontalo Journal of Forestry Research VOLUME 7 NOMOR 2 TAHUN 2024 GORONTALO JOURNAL OF FORESTRY RESEARCH
Publisher : Universitas Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32662/gjfr.v7i2.3765

Propolis is a resinous material that bees gather from specific plant exudate streams and utilize to build hives, caulk or seal crevices, create funnels for bees to enter, store honey and pollen, maintain a warm environment, and function as a natural antibacterial. One type of propolis from the Meliponini Tribe, Propolis Homotrigona Apicalis, is made from resin of the damar tree. This research aims to present scientific data on the effects of propolis as an antibacterial in the human gastrointestinal system. The parameters examined included qualitative phytochemical activity, Porphyromonas sp, Steptococcus sp, and Eschericia sp activity as measured by the minimum inhibitory concentration (MIC) using the agar well method on Mueller Hiton Agar (MHA) media, as well as testing for antibacterial potency and comparing its activity to that of a positive chloramphenicol control. Using the cold maceration technique, 96% EtOH solvent was used to extract propolis over the course of three 24-hour periods. Alkaloids, flavonoids, triterpenoids, and carbohydrates were all active, according to the findings of phytochemical tests. According to the findings of the activity test, Porphyromonas sp, Steptococcus sp, and Eschericia sp all exhibited antibacterial activity. Propolis Homotrigona Apicalis has the potential to be utilized as a treatment that treats diarhea, gum inflammation, and bad breath since it has antibacterial action in these areas as well as a healthy digestive system.

Co-Authors Abdul Rasyid Zarta Abdul Rasyid Zarta Agriandini, Maulina Agus Sulistyo Budi Amirta, Rudianto Ananda, Bayu Susetya Andi Syahid Khalid Assegraff Anjani, Ryan Arief Heru Prianto Aziza, Aziza Azizatul Karimah Binti Khairunnisa Dewi Listyaningrum, Dyah Ayu Dikarulin, Sarah Azzara Dungani, Rudi Edy Budiarso Edy Budiarso Enih Rosamah Enos Tangke Arung Enos Tangke Arung, Enos Tangke Ernita Obeth Erwin Erwin Erwin Erwin Erwin Erwin Esti Handayani Hardi Eva Nurmarini Fahriya Puspita Sari Faizatul Falah Farida Ariani Farida Aryani Farida Fitriani Purba Gina Saptiani Hamka Nurkaya Harlinda Kuspradini Heriad Daud Salusu Heriad Daud Salusu Heriad Daud Salusu Isna Yuniar Wardhani Khairunnisa, Binti Komsanah Sukarti Mark Rayment Muhammad Adly Rahandi Lubis Muhammad Akmal Rizqullah Muhammad Wildan Rizqi Miftahussanadi Nataniel Tandirogang Nissa Nurfajrin Solihat Nugroho, Rudi Agung Nur Indriana Fitriah Nur Indriana Fitriah Nurkadina Nurkadina Octariana Toding Pabita Pasedan, Whicliffe Fiernaleonardo Putri, Tasya Ananda Rico Ramadhan, Rico Rikna Naila Salsabila Rita Rusman Rosamah, Enih Rosamah, Enih Rudianto Amirta Saat Egra Sari, Ayu Mitha Sari, Nur Maulida Sukemi Sukemi Suroto HS Suwinarti, Wiwin Whicliffe Fiernaleonardo Pasedan Wicaksono, Dian Anggoro Widya Fatriasari Wita Pradiani Yani, Sinar Yuliansyah Yuliansyah Yundari, Yundari Zuliani, Nur Endah

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