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All Journal ACTA VETERINARIA INDONESIANA Buletin Peternakan Jurnal Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Majalah Obat Tradisional Jurnal Kedokteran Hewan
Husnurrizal .
Laboratorium Reproduksi, Fakultas Kedokteran Hewan, Universitas Syiah Kuala, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Pengaruh Pemberian PGF2α terhadap Peningkatan Kualitas Spermatozoa Kambing Boerka Amalia Sutriana; Fuza Khoiriah; Husnurrizal .; Tongku Nizwan Siregar; Rasmaidar .; Herrialfian .
Acta VETERINARIA Indonesiana Vol. 8 No. 3 (2020): November 2020
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.8.3.15-21

Abstract

Usaha untuk meningkatkan produktivitas ternak kambing Boerka di antaranya dengan melakukan inseminasi buatan (IB). Untuk meningkatkan kualitas spermatozoa yang akan digunakan untuk IB maka diberikan hormon prostaglandin F2 alfa (PGF2α). Penelitian ini bertujuan mengetahui pengaruh pemberian PGF2α terhadap peningkatan motilitas spermatozoa kambing Boerka. Dalam penelitian digunakan 3 ekor kambing Boerka yang berumur ±2-3 tahun. Pelaksanaan perlakuan dirancang menggunakan pola latin square 3 x 3 sehingga hewan akan menerima suntikan P1 (1,5 ml NaCl fisiologis), P2 (37,5 μg PGF2α), dan P3 (75 μg PGF2α) dengan interval waktu perlakuan adalah 30 menit sebelum koleksi semen. Sampel semen dikoleksi dengan menggunakan vagina buatan dan diamati warna, konsistensi, volume, konsentrasi, motilitas, viabilitas, dan motilitas spermatozoa. Motilitas spermatozoa diamati setelah 4 jam di dalam refrigerator. Data warna dan konsistensi semen dilaporkan secara deskriptif, sedangkan volume, motilitas semen segar, dan motilitas spermatozoa setelah 4 jam di dalam refrigerator dianalisis dengan analisis varian pola bujur sangkar latin (RSBL) yang dilanjutkan dengan uji Duncan. Hasil pengamatan menunjukkan bahwa warna dan konsistensi semen yang dikoleksi pada semua kelompok perlakuan adalah krem dengan konsistensi kental. Rataan (±SD) volume semen; konsentrasi spermatozoa (106/ml); motilitas semen segar (%); dan motilitas semen setelah penyimpanan pada P1 vs P2 vs P3 masing-masing adalah 0,90±0,4 vs 0,70±0,3 vs 0,90±0,3 ml (P>0,05); 2303,33±327,15 vs 2336,67±332,91 vs 2576,67±261,02 (P>0,05); 84,00±5,1 vs 73,33±11,54 vs 80,00±0,0% (P>0,05); 63,67±4,5 vs 53,33±4,7 vs 66,67±2,2% (P<0,05). Disimpulkan bahwa pemberian 75 μg PGF2α dapat meningkatkan motilitas spermatozoa kambing Boerka setelah penyimpanan dalam regrigerator selama 4 jam.
The Effect of PGF2α Injection on Post-Thaw Motility in Sperm of Nubian Goats Aswadi Aswadi; Husnurrizal Husnurrizal; Mulyadi Adam; Tongku Nizwan Siregar
Buletin Peternakan Vol 45, No 1 (2021): BULETIN PETERNAKAN VOL. 45 (1) FEBRUARY 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i1.57335

Abstract

This study aims to determine the effect of PGF2α injection on the post-thaw motility (PTM) in sperm of Nubian goats. Three male Nubian goats (3-4 years) with good reproductive ability were used. This study used a 3 x 3 Latin square design. The experimental animals received a physiological NaCl injection as a control (P1); 37.5 mg of PGF2α (P2), and 75 mg of PGF2α (P3). Semen was collected using an artificial vagina with one-week storage intervals between treatments. The collected semen was then diluted and frozen using a simple freezing method. Observation of semen quality before freezing included macroscopic and microscopic examinations. Macroscopic examination consisted of volume, pH, color, odor, and consistency, while microscopic examination consisted of motility, concentration, viability, and abnormality. PTM examination was done by mixing a drop of sperm suspension and one drop of physiological NaCl on an object glass and covered prior to observation under microscope.. The results were analyzed using a Latin square pattern variant analysis, followed by Duncan's test. The PTM values of sperm (%) of Nubian goats in P1, P2, and P3 respectively are 28.71±10.24, 50.03±13.70, and 54.84±12.04 (P<0.05). Injection of PGF2α to Nubian goats by injection increased the PTM. 
Profil Biokimia Darah Sapi Aceh yang Mengalami Kawin Berulang Cut Nila Thasmi; Husnurrizal Husnurrizal; Muslim Akmal; Sri Wahyuni; Tongku Nizwan Siregar
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.013 KB) | DOI: 10.19087/jveteriner.2021.22.1.26

Abstract

Tujuan penelitian ini adalah mengetahui profil biokimia darah sapi aceh yang mengalami kawin berulang/repeat breeding (RB). Dalam penelitian ini digunakan 16 ekor sapi aceh yang terdiri atas tujuh ekor sapi aceh fertil dan sembilan ekor sapi aceh RB, yang berumur 3-8 tahun dengan skor kondisi tubuh (SKT) 3-4. Koleksi serum dilakukan untuk pemeriksaan profil biokimia darah meliputi kadar kolesterol, protein, dan glukosa. Kadar kolesterol; protein; dan glukosa sapi aceh fertil vs RB masing-masing adalah 185,86±45,34 vs 144,00±40,69 mg/dL; 6,57±1,55 vs 6,96±2,05 g/dL; dan 67,43±13,72 vs 73,78±15,83 mg/dL. Disimpulkan bahwa kadar glukosa sapi aceh RB lebih tinggi dibandingkan sapi fertile, sedangkan kadar kolesterol dan protein relatif sama.
The Effect of Red Betel Leaf (Piper crocatum) and Moringa Leaf Extracts on Endometritis Levels in Aceh Cows Rosmaidar Rosmaidar; Meutia Handayani; Fadillah Fadillah; Teuku Armansyah; Tongku Nizwan Siregar; Hafizuddin Hafizuddin; Husnurrizal Husnurrizal
Majalah Obat Tradisional Vol 26, No 3 (2021)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.64626

Abstract

This study aims to determine the ability of red betel leaf (Piper crocatum) or Moringa leaf extracts to reduce the endometritis level in Aceh cattle. In this study, six Aceh cows aged 3-5 years, weighing 150-250 kg from the Experimental Animal Technical Implementation Unit of Faculty of Veterinary Medicine of Syiah Kuala University were used. The cows were divided into two treatment groups, namely cows with endometritis that were given red betel leaf extract (T1) and cows with endometritis that were given Moringa leaf extract (T2). Examination of the endometritis levels was carried out before and after treatment using the White Side Test (WST) method. The collection of estrus cervical mucus was needed for the WST examination, and heat induction was performed with prostaglandin F2 alpha (PGF2α) at a dose of 25 μg. Collection of cervical mucus was performed 8-12 hours after the initiation of heat. All cows with endometritis were given intrauterine extracts of red betel leaves or Moringa leaves at a concentration of 20% every 24 hours for a week at a solution volume of 20 ml. The data were then analyzed using a paired t test. The mean endometritis levels before and after treatment on K1 vs. K2 were 3.0 and 1.7 vs. 2.7 and 2.7, respectively (P<0.05). It was concluded that red betel leaf extract at a concentration of 20% was more effective in reducing the endometritis level of Aceh cows than Moringa leaf extract.
Accuracy of early pregnancy diagnosis using interferon-tau (IFN-τ) in Aceh cows Fisma Amri; Tongku Nizwan Siregar; Gholib Gholib; Husnurrizal Husnurrizal; Juli Melia; Budianto Panjaitan; Teuku Armansyah
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 31, No 3 (2021): December 2021
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2021.031.03.06

Abstract

Interferon-tau (IFN-τ) is a pregnancy signal produced by embryonic trophoblast cells at the time of implantation in a mammal's endometrial wall, which is useful for conveying a message that the mammal can accept the presence of the foreign object (embryo). This study aims to determine the accuracy of early pregnancy diagnosis in Aceh cows by measuring the concentration of IFN-τ. This study involved eight cows aged 3-5 years, weighing 150-250 kg, clinically healthy, and having normal reproduction (have had calves and at least two regular cycles). All cows were estrous-synchronized twice using PGF2ɑ at a dose of 5 ml with 11 days intervals before artificial insemination (AI). Serum collection was performed on days 14, 15, 16, 17, 18 after AI. Based on ultrasound examination, out of eight cows, four (50%) cows were diagnosed as pregnant, and four (50%) cows were diagnosed as not pregnant. Mean (± SD) IFN-τ of pregnant vs. non-pregnant cows on day 14, 15, 16, 17, and 18 were 14.96±8.65 pg/ml vs. 6.14±5.54 pg/mL; 16.74±5.28 pg/mL vs. 4.44±3.51 pg/mL; 14.33±5.9 pg/mL vs. 5.78±5.20 pg/mL; 13.87±5.42 pg/mL vs. 4.38±3.76 pg/mL; and 13.93±6, 16 pg/mL vs. 3.24±2.63 pg/mL, respectively. The lowest IFN-τ concentration in cows that were successfully pregnant was 7.88±1.84 pg/mL. The best timing of IFN-τ for pregnancy diagnosis was on day 15 after AI, with an accuracy of 87.5%, specificity of 100%, and sensitivity of 80%.
DETECTION OF SERUM MACRO MINERALS CONCENTRATION IN REPEAT BREEDING ACEH CATTLE Cut Nila Thasmi; Husnurrizal Husnurrizal; Muslim Akmal; Sri Wahyuni; Tongku Nizwan Siregar
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.373 KB) | DOI: 10.21157/j.ked.hewan.v14i2.16543

Abstract

The purpose of this study was to determine the macro minerals concentration in serum of repeat breeding (RB) Aceh cattle. In this study 16 Aceh cattle were examined; they consisted of 7 fertile Aceh cattle (P1) and 9 Aceh cattle with RB (P2), all of which were 3-8 years old with abody condition score (BCS) of 3-4. Serum collection was carried out for examining the level of serum minerals, including magnesium, phosphorus, sodium, potassium, chloride, and calcium. Data was analysed using T-test. The levels of magnesium, phosphorus, sodium,potassium, chloride, and calcium in P1 vs P2 were 2.18±0.60 vs. 2.20±0.34 mg/dL; 6.18±1.34 vs. 6.48±0.74 mg/dL; 142.71±5.09 vs.142.44±2.29 mmol/L; 4.81±0.76 vs. 4.76±0.51 mmol/L; 104.57±4.35 vs. 107.67±7.36 mmol/L; and 9.07±0.45 vs. 9.90 ± 0.60 mg/dL (P0.05), respectively. It was concluded that the concentration of serum macro minerals do not affect the incidence of RB in Aceh cattle.
Co-Authors Amalia Sutriana Aswadi Aswadi Budianto Panjaitan Cut Nila Thasmi Fadillah Fadillah Fisma Amri Fuza Khoiriah Gholib Gholib Hafizuddin Hafizuddin Herrialfian . Juli Melia Juli Melia Meutia Handayani Mulyadi Adam Muslim Akmal Muslim Akmal Muslim Akmal Rasmaidar . Rosmaidar Rosmaidar Sri Wahyuni T. Armansyah Teuku Armansyah Teuku Armansyah Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Wahyuni, Sri