YS Darmanto
Program Studi Teknologi Hasil Perikanan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro Jl. Prof. Soedarto, SH, Tembalang, Semarang, Jawa Tengah 50275|Universitas Diponegoro

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Journal : JOURNAL OF COASTAL DEVELOPMENT

THE EFFECT OF DIFFERENT C:N AND C:P RATIO OF MEDIA ON THE CONTENT OF POLYHYDROXYBUTYRATE IN BIOFLOC INOCULATED WITH BACTERIUM Bacillus cereus Supono .; Johannes Hutabarat; Slamet Budi Prayitno; YS Darmanto
JOURNAL OF COASTAL DEVELOPMENT Vol 16, No 2 (2013): VOLUME 16, NUMBER 2, YEAR 2013
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Biofloc technology  has  added values in aquaculture management, both in water quality  management and feeding  management.  As an optional feed, biofloc is capable to enhance growth due to high protein content.  Bacteria, main component biofloc, can produce polyhydroxybutyrate (PHB) as reserve of energy and growth accelerator for fish. The aim of the research were to study the effect of the different C:N and C:P ratio of media on the content of polyhydroxybutyrate in biofloc and to determine optimum media to produce high polyhydroxybutyrate content in biofloc. The experiment was arranged in factorial  with completely randomized design  in  three replications.  Treatments were C:N ratio of 15, 20, 25 and C:P ratio of 75, 100, and 125. The result showed that C:N ratio and C:P ratio of media and their interaction  affect the content of polyhydroxybutyrate in biofloc.  C:N ratio of 20 and C:P ratio of 125 resulted in most polyhydroxybutyrate (29.25±7.376 mg g-1 biofloc dry weight). Ratio of  C:N  of media gave linier and quadratic responses and C:P ratio of media gave linier one. Optimum polyhydroxybutyrate production was obtained at C:N ratio of 20.9 and C:P ratio of  125 resulting in 29.66 mg  g-1 biofloc dry weight (2,97%)
CHARACTERIZATION and QUALITY of SEMI REFINED CARRAGEENAN (SCR) PRODUCTS FROM DIFFERENT COASTAL WATERS BASED ON FOURIER TRANSFORM INFRA RED TECHNIQUE Eko Nurcahya Dewi; YS. Darmanto; Ambariyanto .
JOURNAL OF COASTAL DEVELOPMENT Vol 16, No 1 (2012): Volume 16, Number 1, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Semi Refined Carrageenan (SRC) product is considerably cheaper and easy to produce as a natural polysaccharide hydrophilic in food and other product. The aim of this research was  to evaluate  the quality of two different SCR products come from different  coastal waters  of seaweed culture. The products  were then compared  to commercial SCR on the quality based on their  chemical  quality (Fourier Transform Infra Red, sulphate content and heavy metals) and physical quality  (gel strength and viscosity). The method of FTIR was useful as a quality screening for commercially seaweed culture at different geografic places based on their chemical structure. Raw material that have been used for SCR product  was  Eucheuma cottonii with k-carrageenan type. FTIR spectroscopy showed the molecular present in three different samples are quite similar, it can be found spectra band of 1257,59 cm-1 which referred to esther sulphate, 933,55 cm-1 for 3,6 anhydrogalactose and 848,68 cm-1 assigned to  galactosa-4- sulphate respectively. The SCR product from  different coastal waters were different on their quality.
EFFECT OF SHELLFISH PROTEIN HYDROLYSATE (SPH) OF PEARL OYSTER MEAT ON THE STATE OF WATER AND DENATURATION OF MYOFIBRILS DURING DEHYDRATION PROCESS YS. Darmanto
JOURNAL OF COASTAL DEVELOPMENT Vol 2, No 2 (1999): Volume 2, Number 2, Year 1999
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Pearl oyster meat, considered as the waste product of pearl industry, can be transformed to a useful product troght hydrolysis using protease enzyme. Protein hydrolysate of pearl oyster meat (SPH) was added to myofibrils (Mf) from lizard fish at certain ratios 2.5 – 12.5 g/100 gr Mf, and the changes in the state of water in myofibrils associated with dehydration were analyzed on the basis a water sorption isotherm curve and isosteric sorption heat. Involvement of these change denaturation of myofibrils was investigated with respect to Ca-ATPase activity. The addition of SPH resulted a decreased of water activity, an increase in the amount of monolayer or multilayer water and increase in the isosteric sorption heat, indicative the changes in the state of water in myofibrils. Furthermore, it wa found that SPH has suppressive effects on the denaturation of myofibrils during dehydration process.