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All Journal Jurnal Gizi dan Pangan JURNAL GIZI INDONESIA Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung Journal of Biomedicine and Translational Research Medica Hospitalia
Kis Djamiatun
Magister Ilmu Biomedik Fakultas Kedokteran Universitas Diponegoro
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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Tepung ulat sagu (Rhyinchophorus ferrugineus) imunomodulator Nitric Oxide (NO) sirkulasi mencit terapi antimalaria standar Ariani, Ariani; Anjani, Gemala; Sofro, Muchlis Achsan Udji; Djamiatun, Kis
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 6, No 2 (2018)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.96 KB) | DOI: 10.14710/jgi.6.2.131-138

Abstract

Background : Sago worm flour (Rhyinchophorus ferrugineus)is a coconut beetle larvae that is processed into flour.This flour contains antioxidants as well as arginine, both of which play a role in modulating oxidative stress including NO involved in immunopathology of cerebral malaria.Objectives : To prove the immunomodulator role of sago worm flour in decreasing circulation  NO level at mice  who received standard antimalarial therapy  Dihydroartemisinin Piperaquine (DHP)Methods : This study was randomized control group post test only design by using 23 Swiss mice which divided into 5 group consist of : K(-) normal mice; K(+) inoculated woth Plasmodium berghei ANKA (PbA); X1 received DHP; X2 received sago worm flour; X3 received both of sago worm flour and DHP. All treatment groups X1,X2 and X3 were inoculated by PbA before treatmen were given. Serum circulation NO level was assessed by ELISA. statistical analysis used was One Way ANOVA followed by Bonferroni post hoc test.Results : The mean  NO level in K(-), K(+), X1, X2, and X3 were 1.008 μmol/mL, K(+) 1.338 μmol/mL; while at treatment X1 1.143 μmol/mL, X2 1.410 μmol/mL, dan X3 0.886 μmol/mL. One way ANOVA showed that they were significantly different (p=0.001). Bonferroni post hoc test of X2 was  proportional to K(+) (p=1.000), whole had NO level lower than either K(+)(p=0.009) or X2 (p=0.002). Conclusion : The immunomodulatory effects of sago worm flour (Rhyinchophorus ferrugineus) reduced circulating NO levels in standard antimalarial therapy recipients.
Pengaruh Ekstrak Daun Kersen (Muntingia calabura L) terhadap Kolesterol Darah, Soluble ICAM-1 dan Pembentukan Sel Busa pada Tikus dengan Diet Tinggi Lemak dan Kolesterol Layli, Alvia Nur; Djamiatun, Kis; Kartasurya, Martha Irene
Jurnal Kedokteran Brawijaya Vol 29, No. 3 (2017)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2017.029.03.4

Abstract

Ekstrak daun kersen (EDK) terbukti mempunyai aktivitas antioksidan dan anti-inflamasi yang signifikan namun belum pernah dilakukan penelitian EDK terhadap kadar kolesterol dan inflamasi vaskuler. Penelitian ini bertujuan untuk membuktikan pengaruh EDK terhadap kadar kolesterol darah, kadar soluble ICAM-1 dan pembentukan sel busa aorta tikus wistar yang diberi diet tinggi lemak dan kolesterol (DTL-TK). Penelitian dilakukan dengan Post Test-Only Controlled Group Design. Tikus Wistar jantan sebanyak 24 ekor dirandom menjadi 4 kelompok diantaranya kontrol negatif (KN), kontrol positif (KP), EDK dosis 1 (D1), EDK dosis 2 (D2). KN diberi diet normal, KP diberi DTL-TK saja, D1 diberi DTL-TK dan EDK 250mg/kg BB, D2 diberi DTL-TK dan EDK 500mg/kg BB. Pakan dan EDK diberikan 1 kali dalam sehari. EDK diberikan menggunakan sonde, bersamaan dengan pemberian pakan selama 8 minggu. Hasil penelitian menunjukkan bahwa terdapat perbedaan bermakna (p<0,05) antara KN dan KP pada kadar kolesterol HDL. Terdapat perbedaan bermakna (p<0,05) antara KN dan D1 pada kadar kolesterol total. Terdapat perbedaan bermakna (p<0,05) antara KP dan D1 pada kadar soluble ICAM-1. Terdapat perbedaan bermakna (p<0,05) antara KP dan D2 pada kadar kolesterol total, kolesterol HDL, dan kadar soluble ICAM-1. Tidak terdapat perbedaan bermakna (p>0,05) antara D1 dan D2 pada semua parameter. Hasil pengamatan histopatologi aorta abdominal belum menunjukkan pembentukan sel busa diantara 4 kelompok. EDK dosis 500mg/kg BB dapat menurunkan kadar kolesterol total tikus yang diberi DTL-TK, EDK dosis 250mg/kg BB dan 500mg/kg BB dapat menurunkan kadar soluble ICAM-1 tikus yang diberi DTL-TK. Kata Kunci: Daun kersen, kolesterol, soluble ICAM-1, sel busa
Efek Moringa oleifera terhadap Gula Darah dan Kolagen Matrik Ekstraseluler Sel β Pankreas Diabetes Eksperimental Ambarwati, Ambarwati; Sarjadi, Sarjadi; Johan, Andrew; Djamiatun, Kis
Jurnal Kedokteran Brawijaya Vol 28, No 2 (2014)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2014.028.02.3

Abstract

Daun Moringa oleifera mengandung zat-zat bioaktif yang memperbaiki dan merangsang sel β pankreas untuk mensekresi insulin yang mengatur kadar gula darah. Kelangsungan hidup dan fungsi sel β pankreas memerlukan matriks ekstraseluler (ECM) yaitu fibronektin, laminin dan kolagen. Apakah kadar gula dan kolagen ECM yang mungkin terpengaruh streptozotocin yang merusak sel β pankreas diteliti pada diabetes eksperimental yang diberi perlakukan  M.oleifera. Studi ini menggunakan 24 tikus. Kadar gula darah diukur sebelum dan sesudah induksi streptozotocin 2 hari yang berdampak hiperglikemi, sesudah itu tikus dibagi menjadi 4 kelompok yang terdiri dua grup kontrol yang diterminasi pada hari 0 dan 21 hiperglikemi, dan dua kelompok diperlakukan dengan ekstrak daun M.oleifera dosis 250 dan 500mg/kg BB/hari selama 21 hari. Data dianalisis dengan statistik parametrik dan non-parametrik. Taraf kemaknaan p<0,05. Kadar gula menurun pada masing-masing kelompok perlakukan dosis 250 dan 500mg/BB/kg BB/hari (p=0,000). Kadar gula menjadi normal pada kelompok perlakuan dosis 500mg/kg BB/hari. Ketebalan kolagen ECM pulau Langerhans pankreas tetap normal di semua kelompok penelitian. Ekstrak daun M.oleifera dosis 500 mg/kg BB/hari adalah dosis efektif untuk menormalkan  kadar gula darah pada diabetes eksperimental.Kata Kunci: Diabetes melitus, kadar gula darah, ketebalan kolagen ECM, Moringa oleifera, Streptozotocin,  sel β pankreas
PENGARUH EKSTRAK ETANOL DAUN KELOR (MORINGA OLEIFERA) PADA EKSPRESI INSULIN DAN INSULITIS TIKUS DIABETES MELITUS Sulistyorini, Ratna; Sarjadi, -; Johan, Andrew; Djamiatun, Kis
Majalah Kedokteran Bandung Vol 47, No 2 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.161 KB)

Abstract

Kandungan quercetin pada Moringa oleifera secara ilmiah memiliki potensi sebagai antioksidan dan anti-inflamasi dengan menghambat aktivitas NF-?B, serta triterpenoid menstimulasi regenerasi sel ? pankreas dan meningkatkan sekresi insulin. Penelitian ini bertujuan melihat pengaruh pemberian ekstrak etanol daun Moringa oleifera terhadap insulitis dan ekspresi insulin pankreas tikus Sprague-Dawley jantan. Penelitian dilakukan di laboratorium Unit Pangan dan Gizi, Pusat Antar Universitas Universitas Gadjah Mada serta Laboratorium Histologi dan Biologi Sel Fakultas Kedokteran Universitas Gadjah Mada, periode Mei?Juli 2013. Penelitian ini menggunakan desain randomized post test only controlled group. Hewan coba sebanyak 24 ekor tikus, diperiksa kadar gula darahnya pada hari ketiga setelah induksi streptozotosin, untuk melihat tikus sudah dalam keadaan hiperglikemik. Tikus kemudian  dibagi dalam tiga kelompok: satu kelompok kontrol dan  dua kelompok perlakuan yang  diberi ekstrak etanol daun Moringa oleifera dosis 250 dan 500 mg/kg/hari selama 21 hari. Gambaran histopatologik dinilai  derajat insulitis pada pulau Langerhans dan dinilai ekspresi insulin dengan skor Allred. Uji statistik yang digunakan adalah Kruskal Wallis dilanjutkan Mann-Whitney dengan taraf kemaknaan p<0,05 karena data tidak berdistribusi normal. Derajat insulitis dan ekspresi insulin pankreas tikus pada kedua kelompok perlakuan menunjukkan perbedaan bermakna dibanding dengan kelompok kontrol (p<0,005). Pada penelitian ini dapat disimpulkan bahwa ekstrak etanol daun Moringa oleifera dosis 250 dan 500 mg/kg menyebabkan ekspresi insulin lebih tinggi dan derajat insulitis lebih rendah dibanding dengan kelompok kontrol.[MKB. 2015;47(2):69?76]Kata kunci: Ekspresi insulin, insulitis, Moringa oleifera, streptozotosinEffect of Ethanol Extract of Moringa oleifera Leaves on Insulin Expression and Insulitis in Diabetes Mellitus RatsAbstractQuercetin content in Moringa oleifera has scientific potential antioxidant and anti-inflammation effects by inhibiting the activity of NF-?B. In addition, triterpenoids stimulate pancreatic ? cell regeneration and increases insulin secretion. This study aimed to discover the effect of  Moringa oleifera extract on insulitis and insulin expression in pancreas of  Sprague-Dawley male rats. The study was performed at the laboratory of Food and Nutrition Unit of The Inter University Center of Gadjah Mada University and the laboratory of Histology and Cell Biology Faculty of Medicine  Gadjah Mada University during May?July 2013. This was a randomized post test only controlled group study. Twenty four rats were included. Blood glucose test was performed on the third day after streptozotocin induction to ensure that all rats were in hyperglycemic condition. The rats were then divided into three groups: one control group and two treatment groups receiving Moringa oleifera ethanolic leaves extract for 21 days in doses of 250 and 500 mg/kg/day, respectively. Histopathological analysis of pancreas were performed by evaluating insulitis  based on the infiltration of mononuclear inflammatory cells to the Langerhans islets. Insulin expression was evaluated using Allred score. Statistical analysis used was Kruskal-Wallis test, followed by  Mann-Whitney  with a level of significance of p<0.05. The insulitis degree and insulin expression of the two treatment groups were significantly  different compared to the control group (p<0.005). It can be concluded that Moringa oleifera ethanolic leaf extract doses of 250 and 500 mg/kg/day cause higher insulin expression and lower  insulitis degree than in the control group. [MKB. 2015;47(2):69?76]Key words: Insulin expression, insulitis, Moringa oleifera, streptozotocin DOI: 10.15395/mkb.v47n2.456
Sorghum Tempeh on Cholesterol Levels and Histopathology of Aorta in High-Fat Diet-Induced Rat Model Bintang, Francisca Natalia; Lestari, Endang Sri; Afifah, Diana Nur; Muniroh, Muflihatul; Anjani, Gemala; Syauqy, Ahmad; Djamiatun, Kis
Journal of Biomedicine and Translational Research Vol 10, No 2 (2024): August 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i2.22689

Abstract

Background: Elevated cholesterol levels are associated with hypercholesterolemia, the primary cause of death and lost productivity, and a significant risk factor for the onset of cardiovascular disease. Sorghum is known for its high bioactive components and phenolic compounds, flavonoids, β-glucans, and dietary fiber, which act as anti-cholesterol properties.Objective: This study aimed to analyze the impact of sorghum tempeh on cholesterol levels and histopathology of aorta in rats fed a high-fat diet.Methods: A total of 24 male 8-weeks-old Sprague Dawley rats were randomly assigned to four groups: standard diet group (SD), high-fat diet control group (FD), rats fed high-fat diet + low dose of sorghum tempeh (T1), and high-fat diet + high dose of sorghum tempeh (T2). Measurements of cholesterol levels were determined using the total cholesterol ELISA method. Histopathology of aorta analysis was carried out after four weeks of intervention of the four treatment groups using Hematoxylin-Eosin staining.Results: The average total cholesterol levels post-intervention in the SD, FD, T1, and T2 were 89.986±2.089, 220.365±3.847, 121.161±4.111, and 97.836±2.504 mg/dL, respectively. The results showed that the total cholesterol level significantly decreased (p<0.05) after giving a formula of sorghum tempeh with doses of 0.75 g and 1.50 g per 200 g body weight of rats for four continuous weeks. Histopathology of the aorta in the FD and T2 groups showed a significant difference compared to the SD group. The result which was closest to the SD group was the T1 group.Conclusion: Sorghum tempeh is a high-fiber and antioxidant source that can control hypercholesterolemia by lowering serum total cholesterol. It is also possible to improve histopathology but not yet able to approach normal conditions. The administration of sorghum tempeh with a low dose is sufficient, and further research is still required to determine the effect of sorghum tempeh on aorta histopathology.
The Increased Superoxide Dismuatase (SOD) In Mice Infected by Plasmodium Berghei ANKA Treated with Nanoparticle Extract of Beetroot (Beta Vulgaris L) Hardimarta, Fransisca Pramesshinta; Suromo, Lisyani Budipradigda; Djamiatun, Kis
Medica Hospitalia : Journal of Clinical Medicine Vol. 11 No. 2 (2024): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36408/mhjcm.v11i2.1119

Abstract

BACKGROUND: Malaria infection causes increased free radicals which leads to severity and decreases antioxidant activity, thus increasing the risk of severe malaria complications. Beetroot extract has active compounds that function as anti-inflammatory and antioxidants. Nanoparticles are a technology that can be used to improve drug delivery efficiency in smaller doses. AIMS:  to prove the effectiveness of beetroot extract nanoparticles on SOD levels in mice infected with malaria and treated with artemisinin METHOD: An experimental study using a post-test-only randomized control group design. The research sample used 30 male Balb/c mice divided into 6 groups. Group 1 was the healthy group, group 2 was the infected group without treatment, group 3 was the infected group with artemisinin treatment, group 4 was the infected group with artemisinin treatment and 50 mg/kgBW/day beetroot extract nanoparticles, group 5 was the infected group with artemisinin treatment and 100 mg/kg BW/day beetroot extract nanoparticles, and group 6 was the infected group with artemisinin treatment and 200 mg/kg BW/day beetroot extract nanoparticles. Beetroot extract and artemisinin supplementation were given after parasitemia index > 1% and given for 4 days. On the 5th day after therapy, serum SOD levels were measured using ELISA. RESULT: The measurement of SOD levels in the artemisinin group supplemented with nanoparticle extracts of beetroot at doses 100-200 mg/KgBW were 21,48-21,59 ng/ml. Kruskal Wallis and Mann Whitney test showed that they are significantly higher serum SOD levels compared to the infected mice group (p<0.05). CONCLUSION: Supplementation of beetroot extract nanoparticles has an antioxidant effect by increasing SOD levels in mice infected with malaria and receiving artemisinin therapy.
Golden Berry (Physalis peruviana) Juice for Reduction of Blood Glucose and Amelioration of Insulin Resistance in Diabetic Rats Kinasih, Larasati Sekar; Djamiatun, Kis; Al-Baarri, Ahmad Ni’matullah
Jurnal Gizi dan Pangan Vol. 15 No. 1 (2020)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.108 KB) | DOI: 10.25182/jgp.2020.15.1.37-44

Abstract

The study aimed to gather evidence on the potential of golden berry (GB) juice in improving blood glucose level, insulin level, and insulin resistance in type-2 diabetes mellitus (T2DM) in comparison to quercetin supplement in animal model. This study used true experimental pre-post-test study with control group design. Twenty five Wistar male rats were divided into five groups: healthy group (K-), T2DM positive control group (K+), T2DM group with 1 ml/200 g BW/day of GB juice (X1), T2DM group with 5 ml/200 g BW/day of GB juice (X2), and T2DM group with 6 mg/200 g BW/day of quercetin (X3). The T2DM rasts were obtained from healthy rats induced by high-fat feed and Streptozotocin (STZ). The result showed that various dosages of GB juice (X1 and X2) were able to lower blood glucose level (-79.15; -110.44; -108.20) and HOMA-IR (-2.40; -2.92; -3.02). In addition, it was also able to increase insulin level (0.26; 1.99; 1.42) compared to (K+) group (p<0.05). In conclusion, GB juice was able to lower blood glucose level, insulin resistance, and increase insulin level in T2DM rats. The GB juice dosage of 1 ml/200 g BW/day and 5 ml/200 g BW/day were better in lowering the blood glucose level and improving insulin resistance compared to quercetin supplement.
Co-Authors - Sarjadi, - A.A. Ketut Agung Cahyawan W Ahmad Syauqy Al-Baarri, Ahmad Ni’matullah Andrew Johan Anjani, Gemala Ariani Ariani Bintang, Francisca Natalia Diana Nur Afifah, Diana Nur Endang Sri Lestari Fiqhi Cahyaningrum Rahmawati Hardimarta, Fransisca Pramesshinta Larasati Sekar Kinasih Layli, Alvia Nur Martha Irene Kartasurya Muchlis Achsan Udji Sofro Muniroh, Muflihatul Ratna Sulistyorini, Ratna Sarjadi Sarjadi Suci, Nyoman Suromo, Lisyani Budipradigda Zana Fitriana Octavia