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Journal : Biomolecular and Health Science Journal

Salmonella sp. Detection in Elementary School Street Foods in Surabaya Adelia Anggasta Adzhani; Marijam Purwanta; Ivan Rahmatullah
Biomolecular and Health Science Journal Vol. 1 No. 2 (2018): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.203 KB) | DOI: 10.20473/bhsj.v1i2.9578

Abstract

Introduction: Street foods are food and drink which are processed on the selling spot and/or served as ready-to-consume foods by food vendors to sell to people. Foods and drinks which are rested for too long in the environment will make it possible to get contaminated by pathogen microbes and then results in disease. One of the microbes that is considerably contaminating street foods is Salmonella. We aim to identify Salmonella on drink which are offered in the canteens of Public Elementary School.Methods: This research is design as a descriptive observational, including incubation in Selenite broth, streak in MacConkey agar, and species identification using biochemical tests.Results: There were no Salmonella sp. in drinks sample of street foods which are offered both inside and outside the canteens of Public Elementary School 1 Surabaya, but other gram-negative bacteria contaminated foods.Conclusion: The street foods in elementary school, both the main ingredients and tools used during food processing and serving, still have contamination from pathogen, especially gram-negative bacteria. The vendors and sellers need to be taught how to process and serve foods in better hygiene.
Antibacterial Effects of Fluoride in Streptococcus mutans Growth in Vitro Yura Pradiptama; Marijam Purwanta; Harianto Notopuro
Biomolecular and Health Science Journal Vol. 2 No. 1 (2019): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.927 KB) | DOI: 10.20473/bhsj.v2i1.13232

Abstract

Introduction: Streptococcus mutans is a gram-positive coccus commonly found in the human oral cavity and is a pathogen of dental caries. S. mutans known to form biofilm in infective endocarditis heart-valve. Fluoride usage known to reduce the risk of dental caries. This study aims to analyze how usage of fluoride in inhibiting S. mutans growth.Methods: This study was an experimental study. Antibacterial activity test was performed to evaluate the minimum inhibitory concentration (MIC) using dilution method of sodium fluoride. The minimum bactericidal concentration (MBC) was determined by culturing from the previous dilution test into Chocolate Agar Plate.Results: MIC for sodium fluoride is 4,8 mg/ml and the MBC for sodium fluoride to S. mutans is 4,8 mg/ml. We found S. mutans growth in higher concentration than 19,2 mg/ml. Conclusion: S. mutans was inhibited in dilution test. Growth of the bacteria in higher concentration of sodium fluoride is explained with quasi-irreversible inhibtior effects of fluorida.