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All Journal Jurnal Kedokteran Syiah Kuala Folia Medica Indonesiana Jurnal Biosains Pascasarjana Jurnal Teknologi Laboratorium Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Majalah Biomorfologi (Biomorphology Journal) Indian Journal of Forensic Medicine & Toxicology Cerdika: Jurnal Ilmiah Indonesia
Purwanta, Marijam
Department Of Medical Microbiology, Faculty Of Medicine, Universitas Airlangga, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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IDENTIFICATION OF BACTERIAL CONTAMINANTS ON GLASSES USED BY STUDENTS OF FACULTY OF MEDICINE, UNIVERSITAS AIRLANGGA, SURABAYA, INDONESIA CLASS OF 2016 Hana Letitia Anjani; Marijam Purwanta; Maftuchah Rochmanti
Majalah Biomorfologi Vol. 31 No. 1 (2021): MAJALAH BIOMORFOLOGI
Publisher : Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mbiom.v31i1.2021.18-23

Abstract

Background: People nowadays suffer from refractive disorder that declines the vision. The most common therapy to overcome this problem is to use glasses. The continuous use of glasses and the difficulties on disinfecting the entire surface can cause bacterial contamination. Those bacteria can cause various eye diseases. This fact pushed the researcher to find out if there were bacterial contaminants on glasses used by students of Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia, class of 2016. Objective: To prove the species of bacterial contaminants and pathogenic bacteria on glasses used by students of Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia class of 2016. Materials and Methods: Cotton swab moistened in sterile distilled water was swabbed to 30 glasses on its inside lens and the part touching the nose. The sample was then inoculated on blood agar and Mac Conkey, and incubated for 24 hours in 37oC. Then, the isolates were identified macroscopically and microscopically. Results: The whole sample that had been examined was contaminated with pathogenic bacteria with risk of causing eye diseases. Those bacteria were identified as Bacillus sp. (50%), Pseudomonas sp. (46%), Staphylococcus aureus (20%), and Staphylococcus epidermidis (16%). Conclusion: The result of this research showed that there was contamination by bacteria on glasses used by students of Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia class of 2016. All bacteria found were pathogenic to the eye. This showed that glasses could become a reservoir of the pathogenic bacteria that could potentially cause recurring eye infection.
Aktivitas Antibakteri Ekstrak Etanol Daun Lamtoro (Leucaena Leucocephala) Terhadap Staphylococcus Aureus dengan Metode Dilusi Muhammad Khafidin Al Alim; Marijam Purwanta; Yuani Setiawati
Cerdika: Jurnal Ilmiah Indonesia Vol. 2 No. 2 (2022): Cerdika: Jurnal Ilmiah Indonesia
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.947 KB) | DOI: 10.59141/cerdika.v2i2.345

Abstract

Latar Belakang: Lamtoro (Leucaena leucocephala) merupakan tumbuhan yang umum di indonesia. Tanaman ini selain bisa dimanfaatkan sebagai bahan pangan, daunnya juga sering dipakai masyarakat indonesia sebagai obat luka atau memar. Salah satu bakteri yang umumnya terdapat dalam luka adalah Staphylococcus aureus. Oleh karena itu, peneliti menduga bahwa ekstrak daun lamtoro memiliki aktivitas antibakteri terhadap Staphylococcus aureus. Tujuan: Penelitian ini dilakukan untuk menguji aktivitas antibakteri daun lamtoro terhadap Staphylococcus aureus dengan metode dilusi. Metode: Ekstrak kental lamtoro dibuatkan seri pengenceran pada medium cair. Bakteri uji kemudian ditambahkan dengan proporsi yang sama pada setiap tabung. Tabung dengan konsentrasi ekstrak terendah yang masih terlihat jernih ditetapkan sebagai KHM. Selanjutnya dilakukan streaking pada media biakan. Setelah inkubasi, media dengan konsentrasi terendah yang tidak didapati pertumbuhan koloni ditetapkan sebagai KBM. Hasil: Kekeruhan yang disebabkan ekstrak tidak dapat dibedakan dengan kekeruhan yang disebabkan bakteri, sehingga KHM tidak dapat ditentukan. Dari hasil kultur, didapatkan pertumbuhan koloni pada seluruh media biakan, sehingga KBM juga tidak dapat ditentukan. Hasil hitung koloni menghasilkan bahwa ekstrak tidak membunuh bakteri namun masih dapat menghambat pertumbuhan bakteri tersebut paling optimal pada konsentrasi 125 mg/mL dan 62,5 mg/mL. Kesimpulan: KHM dan KBM ekstrak daun lamtoro terhadap Staphylococcus aureus pada penelitian ini tidak dapat ditentukan. Ekstrak lamtoro tidak membunuh Staphylococcus aureus, namun masih dapat menghambat pertumbuhan bakteri tersebut, paling optimal pada konsentrasi 125 mg/mL dan 62,5 mg/mL.
Antibiotic-Producing Streptomyces sp. Isolated from the Soil of a Mangrove Ecosystem Wiwin Retnowati; Ni Made Mertaniasih; Marijam Purwanta; Nurul Wiqoyah; Atika; Sekar Maharani; Wilda Mahdani
Folia Medica Indonesiana Vol. 59 No. 3 (2023): September
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v59i3.45806

Abstract

Highlights: 1. The unexplored soil of mangrove ecosystems in Surabaya, Indonesia, has the potential to be home to biodiversity,including Streptomyces sp. that can produce antibiotics.2. Streptomyces sp. has antibacterial properties against Gram-positive and Gram-negative bacteria, and the duration ofincubation plays a critical role in regulating the antibacterial activity.   Abstract A mangrove ecosystem in Surabaya, Indonesia, has a high salinity, pH, potassium, phosphorus, and nitrate contents. This ecosystem comprises a mixture of sand, dust, mud, and clay, which has the potential to be a conducive environment for the isolation of Streptomyces. The importance of Streptomyces in biotechnology lies in its ability to produce bioactive secondary metabolites, which represent a valuable reservoir of antibiotics. This research aimed to assess the antibiotic activity exhibited by Streptomyces sp. isolated from the soil of a mangrove ecosystem in Wonorejo, Surabaya, Indonesia. The analysis focused on the potential of Streptomyces sp. to produce antibiotics that work against Gram-positive bacteria (i.e., Staphylococcus aureus ATCC 25923 and Bacillus subtilis) as well as Gram-negative bacteria (i.e., Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Salmonella Typhimurium). The antibacterial activity test was conducted using the modified agar diffusion method. Observations were performed to identify any clear zone formation around the Streptomyces sp. agar colonies with a diameter of 0.8 cm and a height of 3 mm. The clear zone diameter was measured every 24 hours during the 10-day incubation period to assess the diversity of antibacterial activity. The antibacterial profile of Streptomyces sp. exhibited varying levels of activity against different bacterial strains in the tests conducted. The inhibition zone diameters demonstrated the highest levels of activity in Bacillus subtilis (15.9 mm) on day 7, Staphylococcus aureus (27.6 mm) on day 2, Pseudomonas aeruginosa (24.3 mm) on day 7, Escherichia coli (29.2 mm) on day 5, and Salmonella Typhimurium (27.5 mm) on day 7. The results indicated that Streptomyces sp. had inhibitory effects against Gram-positive bacteria as well as Gram-negative bacteria. In conclusion, Streptomyces sp. is a source of biodiversity found in the soil of mangrove ecosystems and has the ability to produce antibiotics.
In vitro Antimicrobial Activity Test of Zingiber officinale var. rubrum Rhizome Extract against Methicillin-Sensitive Staphylococcus aureus (MSSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) Jieputra, Alden; Purwanta, Marijam; Mustika, Arifa; Retnowati, Wiwin
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 15 No. 1 (2024): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V15I12024.57-63

Abstract

Highlights: Staphylococcus aureus, particularly MRSA, had developed rapid resistance against antibiotics like beta-lactams. Red ginger is believed to be antibacterial against MSSA and MRSA in vitro. Red ginger rhizome extracts displayed weak activity against MSSA and MRSA.   Abstract Introduction: Red ginger (Zingiber officinale var. rubrum) is a traditional herb that is believed to possess antibacterial properties. Throughout the years, Staphylococcus aureus has developed resistance to a broad range of antibiotics, including beta-lactams, particularly in the form of Methicillin-Resistant Staphylococcus aureus (MRSA). As treatment options dwindle, it is urgent to formulate novel antibiotics. This study aimed to examine the antibacterial activity of the ginger rhizome ethanol extract against Methicillin-Sensitive Staphylococcus aureus (MSSA) and MRSA. Methods: This study was performed according to the post-test-only control group design. Through a good diffusion assay, the anti-MSSA and anti-MRSA activity of the red ginger extract concentrations (100%, 50%, 25%, 12.5%, and 6.25%) was observed by measuring the diameter of the clear inhibition zones. Dimethyl sulfoxide (DMSO) and an antibiotic disc were added as control groups. Results: The red ginger extracts produced inhibition zones on both MSSA and MRSA. However, the antibacterial activity was considered weak (<12 mm). The concentration of the extract appeared to linearly affect its antibacterial activity against MSSA and MRSA. On MSSA, the 12.5% extract results differed significantly from those of the 100% and 50% extracts. Meanwhile, on MRSA, the extracts seemed to yield significantly different outcomes when compared to each other, except for the comparisons between 50%-25% and 12.5%-6.25%. Conclusion:Zingiber officinale var. rubrum rhizome extracts showed weak antibacterial activity against MSSA and MRSA.
Co-Authors Adelia Anggasta Adzhani Anastassya Yuniar Setyo Putri Anna Roosdiana Ariandi Setiawan Arifa Mustika Arthur Pohan Kawilarang Atika Dwiyanti Puspitasari, Dwiyanti Emeralda Brilian Agnia Felicia Jiwantono Hana Letitia Anjani Harianto Notopuro Ilham Ilham Indiastuti, Danti Nur Inna Fairuuza Firdaus Jieputra, Alden Jusak Nugraha Lilia Putri Pratama Lilik Djuari Muhammad Khafidin Al Alim Ni Made Mertaniasih Nurul Wiqoyah, Nurul Pratama, Dyah Ayu Oktaviani A Qurnianingsih, Ema Rahmatullah, Ivan Rendy, Achmad Nur Ribka Brillyand Angeli Mboe Rifa’I, Muh Husni Rochmanti, Maftuchah Rochmanti Sekar Maharani Wilda Mahdani Winthoko, Eka Nora Vitaloka Aprilia Putri Wiwin Retnowati Yuani Setiawati Yura Pradiptama