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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Veteriner Amerta Nutrition The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy
Sri Estuningsih
Departemen Klinik Reproduksi Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Kampus Darmaga IPB, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health Veterinary Other

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Journal : Jurnal Veteriner

 1 
Stabilitas Mikrob Usus, Histologi Hati dan Ginjal Mencit Setelah Pemberian Ekstrak Pliek u Bumbu Masak Tradisional Aceh (STABILITY OF GUT MICROBIAL AND HISTOLOGY OF LIVER AND KIDNEY OF MICE AFTER ADMINISTRATION OF PLIEK-U EXTRACT Nurliana .; Sri Estuningsih; Sugito .; Dian Masyitha
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.215 KB)

Abstract

Pliek u is one of the traditional spices of Aceh prepared by fermentation of coconut meat which is usedas chicken feed additive too. The stability of gut micro flora and histopathological changes of liver andkidney of mice were detected after three days oral administration of acute single dose of ethanol extract ofpliek u. Nine animals were used and grouped into three; in which group I and II (treatment groups) wereadministered 370 and 733 mg/kg body weight of pliek u, respectively, and group III referred as the control.At the fourth day of experiment, all animals were sacrificed, and their livers, kidneys and intestinal gutwere excised. The gut microbial was measured by Total Plate Count (TPC). Livers and kidneys wereprocessed for paraffin procedure and hematoxyllin-eosin staining. The results showed that the ethanolextract of pliek u has no significant effect (P>0.05) on the TPC of the gut microbial and the structure of theliver and kidney of mice. It can be concluded that administration of the two doses (370 and 730 mg/kg bw)of pliek u did not lowering the numbers of gut microbial and were not toxic to the livers and kidneys of themice.
Gambaran Histopatologi Kauda Epididimis Domba yang Disimpan pada Suhu 4ºC dalam Dulbecco’s Modified Eagle Medium Faisal Amri Satrio; Sri Estuningsih; Ni Wayan Kurniani Karja
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.674 KB) | DOI: 10.19087/jveteriner.2021.22.2.175

Abstract

Penelitian ini bertujuan untuk menganalisis kerusakan jaringan kauda epididimis domba selama empat hari penyimpanan dengan atau tanpa DMEM pada suhu 4ºC. Sebanyak 15 pasang kauda epididimis dikoleksi dari tempat pemotongan hewan dan disimpan dengan cara salah satu dari setiap pasang kauda epididimis dimasukan ke dalam DMEM dan bagian lainnya disimpan tanpa menggunakan DMEM. Preparat histopatologi jaringan kauda epidimis dilakukan setelah penyimpanan pada suhu 4ºC (H-0), lalu dilanjutkan setelah penyimpanan pada suhu 4ºC selama 24 jam (H-1), 48 jam (H-2), 72 jam (H-3), dan 96 jam (H-4). Hasil penelitian menunjukkan bahwa kapsula kauda epididimis secara nyata mengalami penebalan pada H-2 untuk penyimpanan tanpa DMEM dan H-4 untuk penyimpanan menggunakan DMEM (P<0,05). Kerusakan epitel kauda epididimis mengalami peningkatan mulai H-1, namun jumlah kerusakan di H-4 pada penyimpanan menggunakan DMEM lebih sedikit dibandingkan tanpa menggunakan DMEM (P<0,05). Penelitian ini menyimpulkan bahwa penyimpanan kauda epididimis pada suhu 4ºC menggunakan DMEM dapat mengurangi tingkat kerusakan epitel kauda epididimis hingga jam ke-96 dan memperlambat kerusakan kapsula kauda epididimis hingga jam ke-48.
Co-Authors A.S. Satyaningtijas Ahmad Sulaeman Anja Meryandini Arlita Sariningrum Bambang P. Priosoeryanto Bambang Pontjo Priosoeryanto Budi Setiawan Chudahman Manan Chudahman Manan Daldiyono Daldiyono Daldiyono Daldiyono Deni Noviana Dewi R. Agungpriyono Dewi Ratih Agungpriyono Dian Masyitha Eddy Widodo Edward, Syafri Faisal Amri Satrio Hendriana, Okta Hernomoadi Huminto Jeallyza Muthia Azra Maggy T. Suhartono Mokhamad Fakhrul Ulum, Mokhamad Ni Wayan Kurniani Karja Nugrahaeni, Mutiara Nurliana . Rahayu Wulan Rahminiwati, Min Ratih Dewanti -Hariyadi Rika Indri Astuti Robert Utji Satrija, Esdinawan Carakantara Siti Nurjanah sri murtini . Sugito Sugito Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Yaya Rukayadi Yenny Rakhmawati Zuraidah Nasution