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Journal : Science and Technology Indonesia

Bioremediation of Spent Bleaching Earth (SBE) Wastes using Lipolitic Bacteria (Bacillus cereus) with Variation of Inoculum Volume Maria Lusia; Nuni Gofar; Hary Widjajanti
Science and Technology Indonesia Vol. 3 No. 1 (2018): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.782 KB) | DOI: 10.26554/sti.2018.3.1.35-40

Abstract

Spent bleaching earth (SBE) is a solid waste was generated from the CPO refining step into cooking oil. SBE that was discharged directly into the environment has the potential to pollute the environment, because in the SBE waste contained oil and acid residues, which are easily to be oxidized and flammable. Therefore, SBE must be processed first before being discharged into the environment. One way to manage SBE is by bioremediation. Bioremediation is a method on cleaning the environment from contaminants by using biological agents, such as bacteria, fungi etc. The bacterial isolates used in this study were Bacillus cereus. This study aims to obtain the best inoculum and to know the ability of Bacillus cereus bacteria in degrading the oil content in SBE waste. This study used Completely Randomized Design with the volume of Bacillus cereus bacteria inoculum as a treatment, consisting of 6 treatment levels of 0 mL kg-1, 25 mL kg-1, 50 mL kg-1, 75 mL kg-1, 100 mL kg-1, 125 mL kg-1. Each treatment level was repeated 3 times, so taht 18 experimental units were obtained. Observation was done once a week, in a month. Parameters observed were bacterial population, percentage of oil degradation, and oil content degradation. The best treatment result for the bacterial population was obtained at the treatment of 100 mL kg-1, at week 4 which was 7,4 x 108 cfu g-1, and for the oil degradation was obtained at 50 mL kg-1 on the treatment at week 4 as big as 90,43%.
Potential of Anabaena Azollae Extract as Antimicrobial Agent For Paddy Crop Disease Nuni Gofar; Andi Diana; Eka Setianingsih
Science and Technology Indonesia Vol. 3 No. 4 (2018): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (745.042 KB) | DOI: 10.26554/sti.2018.3.4.183-188

Abstract

The research objective was to test antimicrobial activity from compound produced by A. azollae which symbiosis with Azolla sp. toward microbial test of X. oryzae and Pyricularia oryzae as cause of disease on paddy crop. Sampling of Azolla which symbiosis with A. azollae was done in Azolla cultivation pond at Faculty of Agriculture, Sriwijaya University. The available Azolla was consisted of two types, i.e. Azolla pinnata and Azolla mycrophiylla. The extraction of A. azollae from Azolla leaves was done by method of Ultrasound Assisted Extraction (UAE) using ethyl ester solvent and maseration method using methanol solvent. Test media for bacteria and fungus respectively are Mueller Hilton Agar (MHA) and Sabouraud Dextrose Agar (SDA). The diameter of produced clear zone is an indication of extract’s inhibitory power toward bacteria or fungus. The different of inhibitory zone diameter is compared by using t-test. Analysis of active compounds on Anabaena azollae extract was done by using HPLC. Ethyl acetate or methanol extract of A. azollae which symbiosis with A. pinnata or A. microphylla was capable to inhibit the growth of X. oryzae bacterium and P. oryzae fungus. The dominant compounds containing within extract with probability more than 60% and area of more than 10% are consisted of phytol, hexadecanoate acid and 2-(tert-butyl)-4,6-dimethyl phenol.
Co-Authors Abdul Madjid Abdull Madjid Rohim Abdullah Halim Perdana Kusuma Subakti Ace Baehaki Adi Supriyadi Adipati Napoleon Agus Hermawan Albertus Fajar Irawan Albertus Fajar Irawan Ali Amran Amartiya Sari, Shabilla Andi Diana Andika, Gusti Aditya ANDY MULYANA Angga Prasetya Mulya Anita Desiani Anjastari, Devi Annisa Nabila, Annisa Asmak Asmak Ayuputri, Niken Bambang Suprihatin Budi Untari Buyana, Nova Tri Chairu Nisa Apriyani Dade Jubaedah Dade Jubaedah Dedik Budianta Devi Anjastari Diana Sinurat Diana Utama Diana Utama Diana Utama DWI ANDREAS SANTOSA Dwi Paserena, Karinda Eka Setianingsih Eli Sahara Ermatita - Erra Kartika Erra Kartika, Erra Erwin Saputra Fadjar Sidiq Hidayahtullah fitra yosi, fitra Fitralia Elyza Fitri Siti Nurul Aidil Geovani, Dite Ghea Salsabila, Nadhira Gusti Aditya Andika Hary Widjajanti Irmeilyana Kerenila Agustin Kurnia, M Kahfi Aldi Lestari, Primastya Ayu Lestari, Primastya Ayu M. Umar Harun M. Umar Harun Maria Lusia Marini Wijayanti Marsi Meilasari, Nabilla Meisji Liana Sari Miksusanti Miksusanti Muhammad Wahyu Ilahi Muhammat Rio Halim Munandar Munandar Munawar Munawar Muzayyadah, Fathona Nur Nasrul Harahap Neni Marlina Ni Luh Putu SR Novi Wulandari Mustika Nyayu Nurul Husna Primastya Ayu Lestari Putri Nur, Tri Redina An Fadhila Chaniago Renih Hayati Rodiana Nopianti Rohim, A. Madjid Rosmiah Rosmiah, Rosmiah Satria Jaya Priatna, Satria Jaya Siti Komariah Hildayanti Siti Komariah Hildayanti, Siti Komariah Siti Nurul Aidil Fitri Siti Nurul Aidil Fitri Siti Nurul Aidil, Fitri Sofia Sandi Sriati Sriati Sriati Sriati, Sriati Sugandi Yahdin Susilawati Syarifuddin, Fauzi Yusuf Tanbiyaskur Tanbiyaskur Teguh Randi Pradana Tri Putri Nur Utama, Diana Utama, Diana wahyu tri patria Warsito Warsito Widya Irmawati Wuriesyliane Wuriesyliane Yopie Moelyohadi Yopie Moelyohadi Yuli Andriani Yuli Andriani