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GONO SEMIADI
Pusat Penelitian Biologi LIPI, Jl. Raya Jakarta-Bogor, Cibinong 16911
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Veterinary Other

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Journal : Jurnal Veteriner

 1 
Sinkronisasi Estrus dan Inseminasi Buatan pada Rusa Timor Wilmientje Marlene Mesang Nalley; Ristika Handarini; Raden Iis Arifiantini; Tuty Laswardi Yusuf; Bambang Purwantara; Gono Semiadi
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this experiment was to examine the fertility of timor deer (Rusa timorensis) frozensemen by artificial insemination (AI) with intracervical technique after estrus synchronization withControlled Internal Drug Release, for Goat (CIDR-G®). Six adult, healthy, and cycling hinds aged 3 to 4years were used in this experiment. The percentage of estrus hind, onset and duration of estrus wereobserved. Pregnancy diagnosis was conducted using ultrasound scanner (USG) at day 120 afterinsemination. The response of estrus was 82.22 %, the onset of estrus was 25.33 hours after CIDR®withdrawal with the length of estrus was 28 hours. The pregnancy rate at first AI trial under sedation was16.7% (1/6), at second and third without sedation was 60% (3/5) and 100% (2/2) respectively. The durationof pregnancy was 248 to 285 days; with the average calving rate was 50%. The result concludes thatinsemination in hind without sedation was better than with sedation.
KARAKTERISTIK MORFOMETRIK RUSA SAMBAR (RUSA UNICOLOR) SEBAGAI DASAR KRITERIA SELEKSI SIFAT PERTUMBUHAN Wirdateti -; Bram Brahmantiyo; Andi Reksodiharjo; Gono Semiadi; Hadi Dahruddin
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Study on the morphometric characteristic of sambar deer (Rusa unicolor) which will be used as a baseline of growth trait selection had been conducted. The aim of this study is to set up criteria for better selection of sambar deer progeny. Morphometric characteristic observed in this study including : body weight, body length, chest width, chest girth, head length, head width, ear width, and ear length respectively. Result indicated that chest girth correlates significantly with the body weight (y=-108.004+1.875x). In conclusion chest girth can be used as a criteria for selection of growth trait of sambar deer.
Identifikasi Trenggiling (Manis javanica) Menggunakan Penanda Cytochrome B Mitokondria DNA (IDENTIFICATION OF PANGOLIN (MANIS JAVANICA DESMAREST, 1822) USING CYTOCHROME B mtDNA MARKER) Wirdateti .; Gono Semiadi; Yulianto .
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to identify the gentic profile of Malay pangolin (Manis javanica) and originpatterns of confiscated specimens.  Tissue samples of Malay pangolin were collected from several confiscatedmaterials in Tangerang, Medan, and Lampung. Wild collections tissue were also conducted in Lampungand Sukabumi. The study was conducted using  conserved Cytochrome b (Cyt. B) DNA mitochondria(mtDNA). The results showed that based on nucleotide base lentgh of 420 nt, confiscated pangolin wasdistributed in three clades and two groups. Haplotype variations was high, consisted of 19 haplotypes in19 individuals (TR1-TR19). On fisrt clade (TR4,7,16,9,19) high substitution occured in adenin base, cladetwo (TR14,17,1,2,15,3,8,13) high substitution occured in guanin base and clade three  (TR5,6,10,11,12) incytosin. It was concluded that haplotipe variation of each populations  was high and for genetic distancebetween individuals was low. Mutation rates was dominated by transition  from guanine to adenine
Variasi Genetik Trenggiling Sitaan di Sumatra, Jawa, dan Kalimantan Berdasarkan Control Region DNA Mitokndria (GENETIC VARIATION ON CONFISCATED PANGOLIN OF SUMATRA, JAWA, AND KALIMANTAN BASED ON CONTROL REGION MITOCHONDRIAL DNA) Wirdateti Wirdateti; Gono Semiadi
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.693 KB) | DOI: 10.19087/jveteriner.2017.18.2.181

Abstract

High levels of illegal trading on Java pangolin (Manis javanica, Desmarest. 1822) for the basic ingredient of Traditional Chinese Medicine have caused sharp decline in its wild population. The purposes of this study were to assess the level of quality and genetic diversity, and to identify the origin of the confiscated individuals by molecular analysis. The original species used as a control were obtained from known areas in Java, Kalimantan, and Sumatera. Molecular analysis was carried out using non-coding region control region (D-loop) of mitochondrial DNA (mtDNA). The results of phylogenic tree analysis showed that 44 confiscated pangolins were from Kalimantan (24 individuals), from Sumatra (seven individuals), and from Java (13 individuals). As many as 19 haplotypes were found on the basis of their base substitutions consisting of nine from Kalimantan, seven from Java and three from Sumatra. Average genetic distance (d) between those from Kalimantan-Java was d = 0.0121 ± 0.0031; those from Borneo-Sumatra was d =0.0123 ± 0.0038 and those from Sumatra-Java was d = 0.0075 ± 0.038, respectively. Overall genetic distance between populations was d = 0.0148 ± 0.0035, with the nucleotide diversity (ð) of 0.0146. These results indicate that over 50% of pangolins seized came from Kaimantan, and Kalimantan populations show a separate group with Java and Sumatra with boostrap 98%. ABSTRAK Tingginya tingkat perburuan trenggiling (Manis javanica; Desmarest 1822) Indonesia untuk diperdagangkan secara illegal sebagai bahan dasar obat terutama di China, menyebabkan terjadinya penurunan populasi di alam. Tujuan penelitian ini adalah untuk melihat tingkat kualitas dan keragaman genetik trenggiling serta mengetahui asal usul satwa sitaan berdasarkan analisis molekuler. Sebagai kontrol asal usul trenggiling sitaan digunakan sampel alam berdasarkan sebaran populasi yang diketahui pasti yang berasal dari Jawa, Kalimantan, dan Sumatera. Analisis molekuler menggunakan daerah non coding control region (D-loop) mitokondrial DNA (mtDNA). Hasil analisis dari pohon filogeni menunjukkan bahwa dari 44 sampel trenggiling sitaan terindikasi berasal dari Kalimantan sebanyak 24 individu, asal Sumatera tujuh individu, dan dari populasi Jawa 13, sementara rataan jarak genetik (d) antara Kalimantan-Jawa d= 0,0121±0,0031; Kalimantan-Sumatera d= 0,0123±0,0038; dan Sumatera-Jawa d=0,0075±0,038. Jarak genetik secara keseluruhan di antara populasi adalah d= 0,0148±0,0035, dengankeragaman nukleotida (ð) 0,0146. Hasil ini menunjukkan bahwa lebih 50% trenggiling sitaan berasal dari Kalimantan, dan populasi Kalimantan menunjukkan kelompok terpisah dengan Jawa dan Sumatera dengan boostrap 98%.
Suplementasi Ranggah Muda Rusa Sambar Memperbaiki Pertumbuhan Tulang Femur, Bobot Otot, dan Ketahanan Fisik Tikus Putih (THE EFFECT OF SAMBAR VELVET ANTLER SUPLEMENT ON FEMUR BONE, BODY GROWTH, AND PHYSICAL ENDURANCE IN RAT) Gono Semiadi; Raden Taufiq Purna Nugraha; Yuliasri Jamal
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Antlers are deer’s bony organ that follows a cycle of growing, hardening, casting and regroupingwithin a certain period. The effect of consuming velvet antler from temperate origin has beenknown scientifically to have positive effect for rheumatism and metabolic disorder sickness therapy.However, the role of velvet antler originated from tropical deer has not yet been explored. Thisstudy aimed to assess the potential of the velvet antler of sambar deer (Rusa unicolor) which wasexperimentally fed to laboratory rats. The assessment was made based on the animals growth rate(i.e. femur length, weight of testicle, body eight) and physical endurance (i.e. swimming test).Laboratory rats at 21 days old were allocated into four different groups and each group consisted offive rats were fed with powder of soft and hard parts of velvet antler at dose of 0, 1, 2, and 3 g/kgbody weight, respectively. Animals were examined for eight weeks the body weight was examinedweekly and the dose at velvet antler supplement was adjusted accordingly. At the end of the studythe rat were put on endurance swimming test and then euthanized, for measurement of femurbone length and weight of testis. The results showed that there were no differences in the bodyweight. However at dose of 2 g soft part/kg BW indicating a consistently higher live weight gainsacross the observation time. Testis weight showed no significant differences between the treatments,but the length of femur bone showed a significant effect (p <0.05) with the doses level, with thehighest score being at 3 g hard part / kg BW. Physical endurance showed a significant effect (p<0.05) with the doses level, with the level of 1 g soft part/kg BW gave the best performance.
Co-Authors Agustinus Suyanto, Agustinus AMIR HAMIDY Andi Reksodihardjo Andi Reksodiharjo Andy Lukman Affandy Anita S. Tjakradidjaja Artiningsih, Typuk Ba'alwy, Mukhlis Bambang Purwantara Bram Brahmantiyo Bram Brahmantyo Bram Brahmantyo Bram Brahmantyo, Bram Deden Sumirat Hidayat Dedy Darnaedi Dedy Darnaedi Dedy Darnaedi Didid Diapari Eko B. Waluyo Eko Baroto Waluyo EKO BAROTO WALUYO, EKO BAROTO Farida, W Rosa Geoff Asher Hadi Dahruddin Harry Wiriadinata Harry Wiriadinata Harry Wiriadinata, Harry Hellen Kurniati Hellen Kurniati, Hellen Heni Pujiastuti I Ketut Sutama Iman Supriatna Irvan Sidik Irvan Sidik, Irvan Koestoto Subekti, Koestoto MUHAMMAD AGIL Ni Luh Putu Rischa Padmacanthy Ni Luh Putu Rischa Phadmacanty Ni Luh Putu Rischa Phadmacanty Nurhidayat Paul David Muir Prawira, Andhika Yudha R. Iis Arifiantini R. Taufiq Purna Nugraha Raden Taufiq Purna Nugraha Raden Taufiq Purna Nugraha Reksodihardjo, Andi Ristika Handarini Sidik , Irvan Sitanggang, Imas S. Srihadi Agungpriyono Thomas Neville Barry Trilaksono, Wahyu Tuty Laswardi Yusuf Typuk Artiningsih W Rosa Farida Wilmientje Marlene Mesang Nalley Wirdatcti Wirdateti Wirdateti - Wirdateti . Wirdateti Wirdateti Wirdateti Wirdateti Wirdateti Wirdateti Wirdateti Wirdateti, Wirdateti Wirdateti, Wirdatcti Yulianto . YULIASRI JAMAL Yuliasri Jamal Zulkurnia Irsaf