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EFEKTIVITAS WADAH BUNDAR BERARUS TERHADAP PENINGKATAN RESPON IMUN BENIH IKAN MAS (Cyprinus carpio) YANG DIINFEKSI OLEH BAKTERI Aeromonas hydrophila Ibnu Bangkit Bioshina Suryadi; Zaqi Rachman Sidqi; Roffi Grandiosa; Walim Lili
Jurnal Akuakultur Rawa Indonesia Vol 10, No 1 (2022): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jari.v10i1.16850

This research focused on determining the best survival performance of carp (C. carpio) fingerlings in circular containers combined with venturi aerators to generate water current, then challenged by A. hydrophila. The research used an experimental method of Completely Randomized Design (CRD) with four treatments and four replications. The treatment used was the use of venturi aerators on circular containers: without water current as a control (A), with a water current of 0.06 ms?¹ (B), 0.1 ms?¹ (C), and 0.14 ms?¹ (D). The study was initiated by rearing 5-7 cm fish fingerlings for 40 days and followed by a challenge test for 14 days. Carp fingerlings were challenged by A. hydrophila intraperitoneally at a density of 10? CFU ml?¹. Each tank consists of 15 fingerlings. Parameters observed included survival rate, the number of red blood cells and white blood cells, macroscopic symptoms, and water quality. The results showed survival rate in treatment (D) was the best (water current of 0.14 ms?¹), 98% before the challenge period and 95% after the challenge period. In addition, it can increase the hematological parameters as seen from the increase in the number of red and white blood cells after the challenge test, from 1.043 x 106 cells mm³?¹ to 2.36 x 106 cells mm³?¹ and 2.7 x 104 cells mm³?¹ to 9.3 x 104 cells mm³?¹ respectively. And it shows an increased immune response as seen from the recovery acceleration in macroscopic symptoms on the seventh day after A. hydrophila injection. While water quality did not differ between control and treatments.Keywords : current circular generating tank, carp fingerling, Aeromonas hydrophila, immune response

Literature review on fermentation factors of restaurant organic waste affecting feed quality Yuli Andriani; Anggi Adrian Hutapea; Irfan Zidni; Walim Lili; Muhamad Fatah Wiyatna
Depik Vol 10, No 3 (2021): December 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

The high level of public consumption, balanced with the increasingly dense population globally, makes food waste more and more. Food waste is the second-largest contributor to waste in the world. Moreover, the lack of food waste management makes this condition even worse. This food waste can be found, one of which is in restaurants, school canteens, food courts, and others. Fermentation is the process of breaking down complex substances into simpler ones. A fermentation process can help process food waste into various products such as liquid organic fertilizer, compost, biogas, and raw material for fish feed. Through the fermentation process, food waste can be used as an alternative fish feed with high nutritional content to benefit fish cultivators. Fish need many nutrients for their bodies, with food waste that has been processed will meet the dietary needs of fish. The cultivators will benefit more from the cost of feed and the sale of fish that can meet consumer needs.Moreover, the demand for the fish market is increasing every year. Several factors play a role in the success of the food waste fermentation process and the quality of the resulting product to be converted into fish feed raw materials. These factors include incubation time, pH acidity, and microbial factors that can accelerate the spoilage of fish feed raw materials.Keywords:Fermentation,Restaurant wasteFish feedFactorsQuality of feed IngredientsLiterature review on fermentation factors of restaurant organic waste affecting feed quality

Literature review on fermentation factors of restaurant organic waste affecting feed quality Yuli Andriani; Anggi Adrian Hutapea; Irfan Zidni; Walim Lili; Muhamad Fatah Wiyatna
Depik Vol 10, No 3 (2021): December 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.3.23163

The high level of public consumption, balanced with the increasingly dense population globally, makes food waste more and more. Food waste is the second-largest contributor to waste in the world. Moreover, the lack of food waste management makes this condition even worse. This food waste can be found, one of which is in restaurants, school canteens, food courts, and others. Fermentation is the process of breaking down complex substances into simpler ones. A fermentation process can help process food waste into various products such as liquid organic fertilizer, compost, biogas, and raw material for fish feed. Through the fermentation process, food waste can be used as an alternative fish feed with high nutritional content to benefit fish cultivators. Fish need many nutrients for their bodies, with food waste that has been processed will meet the dietary needs of fish. The cultivators will benefit more from the cost of feed and the sale of fish that can meet consumer needs.Moreover, the demand for the fish market is increasing every year. Several factors play a role in the success of the food waste fermentation process and the quality of the resulting product to be converted into fish feed raw materials. These factors include incubation time, pH acidity, and microbial factors that can accelerate the spoilage of fish feed raw materials.Keywords:Fermentation,Restaurant wasteFish feedFactorsQuality of feed IngredientsLiterature review on fermentation factors of restaurant organic waste affecting feed quality

Literature review on fermentation factors of restaurant organic waste affecting feed quality Yuli Andriani; Anggi Adrian Hutapea; Irfan Zidni; Walim Lili; Muhamad Fatah Wiyatna
Depik Vol 10, No 3 (2021): December 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.3.23163

The high level of public consumption, balanced with the increasingly dense population globally, makes food waste more and more. Food waste is the second-largest contributor to waste in the world. Moreover, the lack of food waste management makes this condition even worse. This food waste can be found, one of which is in restaurants, school canteens, food courts, and others. Fermentation is the process of breaking down complex substances into simpler ones. A fermentation process can help process food waste into various products such as liquid organic fertilizer, compost, biogas, and raw material for fish feed. Through the fermentation process, food waste can be used as an alternative fish feed with high nutritional content to benefit fish cultivators. Fish need many nutrients for their bodies, with food waste that has been processed will meet the dietary needs of fish. The cultivators will benefit more from the cost of feed and the sale of fish that can meet consumer needs.Moreover, the demand for the fish market is increasing every year. Several factors play a role in the success of the food waste fermentation process and the quality of the resulting product to be converted into fish feed raw materials. These factors include incubation time, pH acidity, and microbial factors that can accelerate the spoilage of fish feed raw materials.Keywords:Fermentation,Restaurant wasteFish feedFactorsQuality of feed IngredientsLiterature review on fermentation factors of restaurant organic waste affecting feed quality

The effectivity of pandanus leaf extract for the treatment of sangkuriang catfish juvenile Clarias gariepinus infected by Aeromonas hydrophila Sopiah, Siti; Rosidah, Rosidah; Lili, Walim; Iskandar, Iskandar; Suryadi, Ibnu Bangkit
Jurnal Akuakultur Indonesia Vol. 17 No. 2 (2018): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTThe aim of this study was to determine the effective concentration of pandanus Pandanus amaryllifolius leaf extract for the treatment of sangkuriang catfish juvenile infected by Aeromonas hydrophila. The method used in this study was an experimental method with completely randomized design consisted of five treatments and three replications. The treatments were A (control), B (400 mg/L), C (600 mg/L), D (800 mg/L), and E (1000 mg/L) trough immersion for 24 hours through immersion for 24 hours. The fish has infected with A. hydrophila bacteria as much as 20 mL of NaCl/L in 10 L of water medium with a density of 108 CFU/mL through immersion. The observed parameters were clinical symptoms, recovery process, survival rate, and water quality. The results showed that pandanus leaf extract at a concentration of 800 mg/L for 24 hours was effective for treating sangkuriang catfish juvenile that infected by A. hydrophila with a survival rate of 86.67%. Based on the regression analysis, it discovered that the optimum concentration of pandanus leaf extract through immersion for 24 hours was 774.39 mg/L and the predicted survival was 92.16%. Keywords: Aeromonas hydrophila, catfish fingerlings, effective concentration, infection, pandanus leaf extract ABSTRAKPenelitian ini bertujuan untuk menentukan konsentrasi yang efektif dari ekstrak daun pandan wangi Pandanus amaryllifolius untuk pengobatan benih lele sangkuriang Clarias gariepinus yang terinfeksi bakteri A. hydrophila. Metode yang digunakan dalam penelitian ini adalah metode eksperimental rancangan acak lengkap dengan lima perlakuan dan tiga ulangan. Perlakuan penelitian adalah A (kontrol), B (400 mg/L), C (600 mg/L), D (800 mg/L), dan E (1000 mg/L). Ikan uji diinfeksi bakteri A. hydrophila sebanyak 20 mL NaCl/L dalam 10 L media air dengan kepadatan 108 CFU/mL melalui perendaman selama 24 jam. Parameter yang diamati meliputi gejala klinis, waktu pulih, kelangsungan hidup, dan kualitas air. Hasil penelitian menunjukkan bahwa penggunaan ekstrak daun pandan wangi pada konsentrasi 800 mg/L selama 24 jam efektif untuk pengobatan benih lele sangkuriang yang terinfeksi bakteri A. hydrophila dengan tingkat kelangsungan hidup sebesar 86,67%. Berdasarkan analisis regresi diketahui bahwa konsentrasi optimum penggunaan ekstrak daun pandan wangi melalui perendaman selama 24 jam yaitu 774,39 mg/L dan prediksi kelangsungan hidup ikan adalah 92,16%. Kata kunci : Aeromonas hydrophila, benih lele sangkuriang, ekstrak daun pandan, infeksi, efektifitas konsentrasi

The Effectiveness of Chitosan to Increase Body Resistance of Sangkuriang Catfish (Clarias gariepinus) Against Aeromonas hydrophila Attack Mahatma Kresnha Radityo; Rosidah; Walim Lili; Roffi Grandiosa Herman
Jurnal Akuakultur Indonesia Vol. 21 No. 2 (2022): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.21.2.93-108

This research aims to determine the optimal dose of chitosan that is appropriate to be given to commercial feed in increasing the immune system of sangkuriang catfish fry. The research was carried out in April – June 2021 at the Aquaculture Laboratory and Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University. This research used an experimental method of Completely Randomized Design (CRD) with five treatments and three replications. The treatment used was the addition of chitosan to feed with doses of 0 g (A), 2.5 g (B), 5 g (C), 7.5 g (D), and 10 g (E). Maintenance of test fish for 14 days. Experimental fish were infected by Aeromonas hydrophila bacteria with a density of 108 CFU/ml with 10 ml immersion method. Parameters observed included white blood cells, leukocyte differential, clinical symptoms, survival and water quality. Data on white blood cell count, and survival were analyzed by ANOVA, if there was a significant difference, Duncan's test was performed. Leukocyte differential data, clinical symptoms and water quality were analyzed descriptively. The results showed that the addition of chitosan at a dose of 10 g was effective for improving the immune system performance of catfish fry. During 14 days of chitosan administration, the white blood cell count increased by 41.60% from the value of 13.96 × 104 cells/mm3 to 23.90 × 104 cells/mm3. The 10 g chitosan treatment was an effective dose to stimulate differential leukocytes in catfish to improve the immune system, it was also marked by the fastest clinical symptom recovery period and the highest survival rate after the challenge test, which was 96.6%.

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