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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Majalah Ilmiah Peternakan Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Swabumi (Suara Wawasan Sukabumi) : Ilmu Komputer, Manajemen, dan Sosial Tropical Animal Science Journal Abditeknika - Jurnal Pengabdian Kepada Masyarakat
A. GUNAWAN
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Energy Social Sciences Veterinary

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Journal : Tropical Animal Science Journal

 1 
Proteomic Analysis of Pesisir Bull Sperm in Different Age Groups for the Identification of Reproductive Function Proteins Anwar, P.; Maulana, T.; Sumantri, C.; Arifiantini, I.; Gunawan, A.
Tropical Animal Science Journal Vol. 48 No. 5 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.5.393

Abstract

Pesisir cattle are native to Indonesia and originate from West Sumatra. It is known for its high environmental adaptability and can be further developed using a proteomic approach. The proteomic approach for testing the fertility of Pesisir bulls is an important factor in determining a potentially superior breed. Proteomic studies of reproduction in Pesisir bulls have not been widely conducted. This study aimed to identify and characterize functional sperm proteins in Pesisir bulls that are associated with reproductive processes. Semen samples were collected from 12 pesisir bulls aged 2–4 years and grouped into three age categories. Protein profile analysis was performed using polyacrylamide gel electrophoresis with 1D-SDS-PAGE, followed by proteolytic digestion of the proteins in the gel and protein identification using LC-MS/MS analysis. Protein functions were predicted based on analysis of biological annotations generated using UniProt, Venny, PANTHER, and STRING. Based on the evaluation of fresh semen, sperm motility in Pesisir bulls was found to increase with age. Proteomic analysis successfully identified 334 proteins in the sperm of Pesisir bulls. Among these, two proteins, ZPBP and SPACA3, were identified as involved in reproductive and fertilisation processes. Functionally, both proteins play crucial roles in acrosomal events during fertilisation. Gene Ontology analysis showed that most proteins in sperm are involved in various biological processes, including cellular activities, metabolic processes, and molecular functions related to catalytic activities. In conclusion, SPACA3 and ZPBP proteins were identified as potentially involved in reproductive processes and may serve as fertility markers in Pesisir bulls.
The Association of IGFBP7 Gene Polymorphism on Lamb Meat Quality in Javanese Thin-Tailed Sheep Komarudin; Listyarini, K.; Budiman, C.; Sumantri, C.; Gunawan, A.
Tropical Animal Science Journal Vol. 47 No. 4 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.4.448

Abstract

The insulin-like growth factor binding-protein 7 (IGFBP7) gene is one of the potential genes related to meat quality. The objective of the current study was to evaluate polymorphism of the IGFBP7 gene (g.72351183A>C) and its association with meat quality traits in the Javanese thin-tailed (JTT) sheep. A comprehensive analysis was conducted on 88 JTT male sheep to examine their fatty acid composition, carcass characteristics, carcass retail cuts, and the physical properties of lamb meat. The polymorphism was detected using the PCR-RFLP technique. The association between the IGFBP7 gene polymorphism and the observed variables of meat quality was evaluated using one-way analysis of variance (ANOVA). The study results indicated that the IGFBP7 gene was polymorphic in JTT sheep population, with the allele distribution conforming to Hardy-Weinberg equilibrium. The AA genotype was found to be predominant. The IGFBP7 gene variants were associated (p<0.05) with erucic acid, linoleic acid, eicosapentaenoic acid, and total polyunsaturated fatty acid (PUFA). Sheep possesing the CC genotype exhibited the highest levels of linoleic acid, eicosapentaenoic acid, and total PUFA in comparison to those with AA and AC genotypes. However, the IGFBP7 gene polymorphism was not associated with carcass characteristics, carcass retail cuts, and physical properties of meat. These findings suggest that the IGFBP7 gene is a promising candidate marker for improving fatty acid composition in JTT sheep.
Co-Authors . Aryogi . Hartati A. Anggraeni A. Irianto Amin, M. F. Andika Purnama Anuraga Jayanegara Anwar, P. Arief, I. I. Azizah, D. Budiman, C. C. SUMANTRI C. Sumantri Chalid Talib Desi Susilawati, Desi E. Gustia F. Amri Fastawa, R. Furqon, A. Galib, I. Gunawan Gunawan Gunawan Gunawan Harahap, R. S. Hikmawaty, Hikmawaty I. Arifiantini Istiqlal, R. F. Jakaria Jakaria K. JAMAL Komarudin Kosim, A. Listyarini, K. M. F. Al-Habib Mulyono, R. H. N. Azmi, N. N. Ulupi Pati, N.K. Pribadi, Denny Putera, B. W. R. Priyanto R. R. Noor, R. R. R.I. Arifiantini Ramdhan Saepul Rohman, Ramdhan Saepul Roosita, K. S. Darwati S. Murtini Sri Mulatsih Suhita, R. L. T. Maulana, T. T. Suryati Ulupi, N. Widiono, Widiono Y. Parwoto Yanthi, N. D. z. Abidin